Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,387,114 C→A 6.8% V338L (GTA→TTA)  EO946_RS12195 ← acetyl‑CoA C‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,387,1140CA 6.8% 513.0 / 2.2 193V338L (GTA→TTA) EO946_RS12195acetyl‑CoA C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (87/81);  new base A (20/2);  total (109/84)
Fisher's exact test for biased strand distribution p-value = 3.93e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

AGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAG  >  NZ_CP034943/2386977‑2387251
                                                                                                                                         |                                                                                                                                         
aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACtt                                                                                                                                         >  2:3846683/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTGATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCCCCGGTTACCTTTAAtttttaa                                                                                                                                    >  2:353889/1‑138 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGGGTATTCTCGCACCGCGTGCGCCGATCGGGGGGCCGCGGGCGATCGCACCACCGGTTTCATTTACTTTTTCa                                                                                                                                    >  2:656662/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCCCCCCTTGCGCCGCTCGGGTGGCCGCGGGCGATCGCACCACCCGTTTCCTTTAATTTTTCa                                                                                                                                    >  1:3066334/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCCGTTACATTTACtttttaa                                                                                                                                    >  2:1760978/1‑138 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATAGGGTGGCCGAGGGCGCTCGCACCACCGGTTACATTTACTTTTTCa                                                                                                                                    >  2:4001706/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTAAAGCACCGCTTTCGCCCAAAGGGTGGGCGAGGGCGATCGCACAACCCTTTAACTTTAAATTTTCa                                                                                                                                    >  2:131438/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAACTGTCATGAGTATTCGCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCCCCACCGGTTGCATTTACTTTTTCa                                                                                                                                    >  2:2860849/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAAAAAGTGTCATGCGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATGGCACCCCCGTTTACATTTACTTTTTCa                                                                                                                                    >  2:2982272/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGGAAACAAGTGTCAAGAGTATTGTCGCACCGCTGGCGCCGATAGGGGGGCCGAGGGCGATCGCACCACCGTTTACAATTAATTTTTCa                                                                                                                                    >  2:2082331/1‑140 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTTATTTGTAAACAAGTGTGATGAGTATTATGGCACCCCGTGCGCCGCTCGGGTGGGCGCGGGCGATCGCACCACCGGTTAAATTTTATTTTTTAAGATc                                                                                                                               >  1:3308035/1‑140 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAACAAATGTGATGTTTATTGTCGCACCGCGTTCGCCGATCGGGTGGCCGAGGGGGATCGCACCACCCGGTTCAGTTAATTTTTTAAGAtg                                                                                                                               >  1:758368/1‑139 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAAATCGTAAACAAGTGTCATGAGGATTCGCCCACCGCCTGCGCCGATCGGGGGGGCGAGGGCGATCGCACCACCGGGTCGATGTCCTTTTTGAAGATc                                                                                                                               >  2:794154/1‑140 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTAGTAAAAAACTGTGATTAGTAATGTCGCACCGCGTGCGGCGCTGGGGTGGGCGCGGGCGGTCGCACCACCGGTTACATTTAATTTTTGAAGAtg                                                                                                                               >  1:3353723/1‑139 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTATTTCGTTAACAAGTGTCAAGAGGATTCTCGCACCCCGTGCGCCGAGCGGGTTGGCGGGGGGGGTGGCCCCCCCGGGTGGAGTTACATTTTCAAGCTc                                                                                                                               >  1:1587761/1‑140 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAATGTCATGAGTATTCTCGCCCCCCGTGGGCCGATCGGGTGGCCGAGGGCGGTCGCACCACCGGTTAACTTTTCTATTTAAAGAta                                                                                                                               >  1:1468972/1‑139 (MQ=255)
             cAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTTATTCGTAAACAAGTGTCATGGTTATTCTGGCACCCCTGGGGCCGATCGGGTGGGCGAGGGCGATCGCACCACCGGGTTCATTTACTTTTTGAAGATAGaa                                                                                                                            >  1:4047359/1‑140 (MQ=255)
               aaTGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACCAGTGTCATGAGTATTGTCGCACCGCTTGCGCCGATCGGGGGGCCGAGGGCGCTCGCACCACCGGTTACCTTTAATTTTTCAAGATAGAAAc                                                                                                                          >  2:1787039/1‑140 (MQ=255)
                   gcggcAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACcgac                                                                                                                      <  1:3974501/140‑1 (MQ=255)
                     ggcAACACCCAGTCCCCCTCCCCGTCGCGTTTATTAGTAAACAAGTGTCATGGGTTTTGTCGCCCCGCGTGCGCCGCTCGGGTGGCCGCGGGGGATCGCACCACCGTTTACATTTAATTTTTGAAGATAGAAAAcgacga                                                                                                                    >  1:1218894/1‑140 (MQ=255)
                     ggcAACACCCAGTCCCCCTCCCCGTCGCGTTTATTAGTAAACAAATTTGATTAGTTTTATGGCACCCCGTGCGGCGCTCGGGGGGGCGGGGGGGAGCGCCCCCCCGGTTTCATTTTATTTTTTAATATCGAAACccacca                                                                                                                    >  1:3746105/1‑138 (MQ=255)
                             ccAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                                                                                                            <  1:3846683/140‑1 (MQ=255)
                                gTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACAt                                                                                                         <  2:1675951/140‑1 (MQ=255)
                                 tCCCCCTCCCCGTCGCTTTTATTCGTTAACAATTGTGCTGAGTATTATCGCCCCGCTTGCGCCGATGGGGGGGCCGAGGGCGATCGCACCACCGTTTGGATTTAATTTTTGAAGATCGAAACCGGCGATTTTTTTAAAtt                                                                                                        >  1:3946881/1‑139 (MQ=255)
                                     ccTCCCCGTCGCGTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACAATTACTTTTTAAAGATCGAAACCCACGATTTTTTTACATGTCaa                                                                                                    >  2:3318845/1‑140 (MQ=255)
                                      cTCCCCGTCGCTTTAATTCGTAAAAAAGTGTCATGAGTATTATCGCACCGCTTGCGCCGATAGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTAAAGATCGAAACCGACGATTTTTTTACATGTGaaa                                                                                                   >  2:700292/1‑140 (MQ=255)
                                        ccccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGGCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAATATCGAAACCGACCATTTTTTTACATGTCaaaaa                                                                                                 >  2:2809850/1‑139 (MQ=255)
                                         cccGTCGCGTTAATTCGTAAAAAACTGTCAAGAGTATTCTCGCACCGCGTGCGCCGATCGGGTGGGCGAGGGCGATCGCCCCACCGGTTACCTTTTATTTTTCAAGATCGAAACCGACGATTTTTTTTCATGGGAAaaca                                                                                                >  2:1496144/1‑140 (MQ=255)
                                          ccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa                                                                                               <  2:2094289/140‑1 (MQ=255)
                                          ccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa                                                                                               <  1:1831429/140‑1 (MQ=255)
                                           cGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaac                                                                                              <  1:2773440/140‑1 (MQ=255)
                                            gTCGCTTTAATTCTTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGGCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAacaaca                                                                                             <  2:3641254/140‑1 (MQ=255)
                                            gTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAacaaca                                                                                             <  2:1400566/140‑1 (MQ=255)
                                            gTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAacaaca                                                                                             <  2:3127148/140‑1 (MQ=255)
                                             tCGCTTTTATTCGTAAAAAAATGTGATGCGTATTCTCGCACCGCTTGCGCCGATAGGGTGGCCGTGGGCCGGCGCACCCCCGTTTACATTTACTTTTTAAAGATCGCAACCGCCGAATTTTTTTAATGTCAAAAAAAAAg                                                                                            >  2:1212996/1‑140 (MQ=255)
                                                  ttAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCa                                                                                       <  1:3214273/140‑1 (MQ=255)
                                                  ttAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCa                                                                                       >  2:2392859/1‑140 (MQ=255)
                                                  ttAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCa                                                                                       <  1:1400162/140‑1 (MQ=255)
                                                   tAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCaa                                                                                      <  1:2971444/140‑1 (MQ=255)
                                                    aaTTCGTAAACAAGTGTCATGAGTAGTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTACAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCaac                                                                                     <  1:3000417/140‑2 (MQ=255)
                                                        cgtcaccAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGAACGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCt                                                                                 <  1:939916/134‑1 (MQ=255)
                                                        cGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTTGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCt                                                                                 <  1:1043443/140‑1 (MQ=255)
                                                          tAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTc                                                                               >  2:3279733/1‑140 (MQ=255)
                                                            aaCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAt                                                                             <  1:675601/140‑1 (MQ=255)
                                                             aCAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAtt                                                                            <  1:656662/140‑1 (MQ=255)
                                                                    tCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAATCAAAAGCTTAATTTAATTCa                                                                     >  2:188435/1‑140 (MQ=255)
                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                   <  2:1456165/140‑1 (MQ=255)
                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                   <  1:2392859/140‑1 (MQ=255)
                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                   <  1:3405574/140‑1 (MQ=255)
                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACAGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                   <  1:1283655/140‑1 (MQ=255)
                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACAACCGTTTACTTTTACTTTTTCATGATCGAAACCGACGATTTTTTTACATGTCATAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                   <  1:478856/140‑1 (MQ=255)
                                                                       tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                  <  1:2831292/140‑1 (MQ=255)
                                                                         aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                                                                >  1:1056969/1‑140 (MQ=255)
                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                              >  2:3703457/1‑140 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                            <  2:3353723/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                            <  2:1468972/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                            <  1:1911393/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                            <  2:3752463/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                            <  2:4247823/140‑1 (MQ=255)
                                                                              tctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCaa                                                           >  2:2768150/1‑140 (MQ=255)
                                                                               ctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAat                                                          <  2:3746105/140‑1 (MQ=255)
                                                                                tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGTAGCAAACGCGTCATTTACTTCAAACAAGTCAata                                                         >  2:3151323/1‑140 (MQ=255)
                                                                                tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAata                                                         >  2:485567/1‑140 (MQ=255)
                                                                                tcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTGCATTTACTTCCAACAAGTCAata                                                         >  2:2171881/1‑140 (MQ=255)
                                                                                  gCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTCCATTTACTTTTTCAAGATCGAAACCGACGATTGTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAGCACGTCCATATc                                                       >  2:2662850/1‑140 (MQ=255)
                                                                                  gCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACAGACGATTTTTTTACATGTCAAAACAACAGAAGCACACGCTTCATTTACTTCAAACAAGTCAATATc                                                       >  1:1127515/1‑140 (MQ=255)
                                                                                   cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCt                                                      >  1:2532340/1‑140 (MQ=255)
                                                                                   cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCt                                                      >  2:4069397/1‑140 (MQ=255)
                                                                                   cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCt                                                      >  1:3874806/1‑140 (MQ=255)
                                                                                   cACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCt                                                      >  2:3207675/1‑140 (MQ=255)
                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACCAGTCAATATCTTg                                                    <  1:17829/140‑1 (MQ=255)
                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg                                                    <  2:604017/140‑1 (MQ=255)
                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACCTTTACTTTTTCAAGATCGACACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                 >  2:3217183/1‑140 (MQ=255)
                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                 >  1:221735/1‑140 (MQ=255)
                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                 >  2:752403/1‑140 (MQ=255)
                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                 >  2:302233/1‑140 (MQ=255)
                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                 >  2:474781/1‑140 (MQ=255)
                                                                                        cTTGCCCCGATCGGGTGTCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGTAc                                                 >  2:4207596/1‑140 (MQ=255)
                                                                                                gATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAAc                                         <  2:3183864/140‑1 (MQ=255)
                                                                                                  tCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAtt                                                                            <  2:915775/103‑1 (MQ=255)
                                                                                                  tCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAtt                                                                            <  2:3592244/103‑1 (MQ=255)
                                                                                                  tCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAtt                                                                            >  1:915775/1‑103 (MQ=255)
                                                                                                  tCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCAtt                                                                            >  1:3592244/1‑103 (MQ=255)
                                                                                                   cGGGTGGCCGAGGGCGATCGCAACACCTTTTACATTTAATTTTTCAAGATGGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGt                                      <  1:188435/140‑1 (MQ=255)
                                                                                                     ggTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTc                                    <  2:1127515/140‑1 (MQ=255)
                                                                                                      gTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                            <  1:2941803/115‑1 (MQ=255)
                                                                                                           cGAGGGCGATCGCACCACCGTTGACATTTACTTTTTCAAGATCGAAACCGACGATTTGTTTACATGTCACAACATCAGAAGCAACCGCTTCAGTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCttgtgc                              >  1:1020352/1‑136 (MQ=255)
                                                                                                            gAGGGCGATCTCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCa                             <  2:2963262/140‑1 (MQ=255)
                                                                                                              gggCGCTCGCACCACCGTTTACAATTACTTTTTACACCTCGAAACCGACCATTTTTTTACATGTCAAAACAACAGAATCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAgg                           <  1:3781714/140‑1 (MQ=255)
                                                                                                              gggCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAgg                           <  2:3877827/140‑1 (MQ=255)
                                                                                                                gCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGt                                      >  2:1371836/1‑127 (MQ=255)
                                                                                                                   aTCGCACCACCGTTGACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCtt                      >  1:1805907/1‑140 (MQ=255)
                                                                                                                    tCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTg                     >  1:3115021/1‑140 (MQ=255)
                                                                                                                    tCGAACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTCGGACGGTTAAACCGTTCTTTTTCAGGAGCTTg                     >  2:416332/1‑140 (MQ=255)
                                                                                                                             tgTTTTCATTTATTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACATCAGAAGCAAAAGCTTCATTTACTTCAAACAAGTCAATATATTGGTCGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCa            <  2:2863128/139‑1 (MQ=255)
                                                                                                                             cGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCa            <  1:981085/140‑1 (MQ=255)
                                                                                                                                    aTTTACTTTTTCAATATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTACATTTTCTTCAAACAAGTGAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGTGCTTGGTAATTTCAAACCCCg     >  1:170169/1‑140 (MQ=255)
                                                                                                                                      ttACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGa   >  1:1520194/1‑140 (MQ=255)
                                                                                                                                       tACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGCAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAg  <  2:2502708/140‑1 (MQ=255)
                                                                                                                                       tACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAg  <  2:3874806/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
AGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAG  >  NZ_CP034943/2386977‑2387251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: