Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,644,019 A→C 6.5% V322V (GTT→GTG ruvB ← Holliday junction branch migration DNA helicase RuvB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,644,0190AC 6.5% 541.2 / 2.2 209V322V (GTT→GTGruvBHolliday junction branch migration DNA helicase RuvB
Reads supporting (aligned to +/- strand):  ref base A (131/54);  new base C (2/22);  total (133/76)
Fisher's exact test for biased strand distribution p-value = 3.35e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

TTTCACAAAAATATCTCCCGGCATCTTGCCGACAAAATGCAATACTGCTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGTATCGAGACCCACCGGGC  >  NZ_CP034943/2643883‑2644155
                                                                                                                                        |                                                                                                                                        
tttCACAAAAATATCTCCCGGCATCTTGCCGACAAAATGCAATACTGCTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACg                                                                                                                                       >  1:2825202/1‑140 (MQ=255)
  taacaAAAATATCTCCCGTCATCTTGCCTACAAAATTCAATACTGCTCCGATCATAATAAAAACAGCTCCAATAATCATAATGATCTTAGGGAATTCTGTCATATCGTGTATCTTATATTTTTATATGATGATaatagtc                                                                                                                                     <  2:2305431/138‑6 (MQ=255)
  taaaaaaaaTATCTCCCGGCATCTTGCCGACAAAATGCAATAGTGCTCCGATGATAAGCAAAACAGCTCCAAGAATAATATTGATCTTAGGGAATTCAGTCATATGGGGGAGCCTCCATTTGGAAATGATGATAAACGGc                                                                                                                                     <  1:3391794/136‑1 (MQ=255)
  tCACAAAAATATCTCCCGGCATCTTGCCTACAAAATGCAATACTGCTCCGATGATAAGAAAAACAGCTCCAACAATAATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGTTGATAAACGGc                                                                                                                                     <  2:4021552/140‑1 (MQ=255)
  tCACAAAAATATCGCCGGGAATCGTGCCTAAAAAATGCAATACTGCTCCGATGATAAGAAAAACAGCTCCAATAATAATAATGATCTTAGGTAATTCAGTCATTTGGGGGAGCCTCAATTTGGAAATGATGATACACGGc                                                                                                                                     <  1:2626332/140‑1 (MQ=255)
             tctcCCGGCATCTTGCCGACAAAATGCAATACTGCTCCGATGATAACCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGa                                                                                                                          <  2:956288/140‑1 (MQ=255)
                         ttGCCGACAAAATGCAATACTGCTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGACTTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCcgcg                                                                                                              >  2:1463558/1‑140 (MQ=255)
                         ttGCCGACAAAATGCAATACTGCTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCcgcg                                                                                                              >  2:1976624/1‑140 (MQ=255)
                           gCCGACAAAATGCAATACTGCTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCgcggc                                                                                                            >  1:2911655/1‑140 (MQ=255)
                                aaaaaTGAAATAGTGCTCCGATTATAAGCAAAACAGCTCAAAGAATAATAATGATCTTAGGTAATTCAGTCATATCGGGGAGCCTCCATTTGTAAATTATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCt                                                                                                       <  1:3684447/139‑1 (MQ=255)
                                              gCTCCGATGATAAGCAAAACCGCTCCAAGACGCATAATGAACTTAGGGAAGTCAGTCATTTCGGGCACCCTCAAGTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGa                                                                                         <  2:623085/140‑1 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGTAATGATGATAAACGGCCGGTGTCACGACCCGTCCCCGCGGCGTGCTTTGAAGAACTCCGAt                                                                                        >  2:3220776/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGACAGGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGACTAAATCCGAt                                                                                        >  1:1085218/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCCCGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:3216945/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:3149300/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  2:1517264/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  2:3087335/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:1675643/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:3506700/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:4245192/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:242374/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  2:1286884/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:876779/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:978910/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  2:835381/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:2755351/1‑140 (MQ=255)
                                               cTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:25187/1‑140 (MQ=255)
                                               cTCCGATGATAACCAAAACAGCTCCAAGACTCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAACTGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGAt                                                                                        >  1:3642212/1‑140 (MQ=255)
                                                  cGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGAGACACGGCCGGTGTCACGATCCTTCCCCGCGGCGGTCTTTGAATACATCCGaggtg                                                                                     >  2:3942814/1‑136 (MQ=255)
                                                   gattatAAGCAAACCAGCTCCAATAATCATAATGATCTTAGGGAATTCAGTCATCTCGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGc                                                                                    <  1:1533515/140‑1 (MQ=255)
                                                   gattatAAGAAAAACACCTACAAGAATCATAATGATATTTGGGAATTCAGTCATATCGGGGAGCCTCAATTGGCAACTCATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGc                                                                                    <  1:522051/140‑1 (MQ=255)
                                                   gatgatAAGCAAAACAGCTCCAAGAATCATATTGTTCTTAGGTATTTCAGTCTTATGGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGc                                                                                    <  1:2664820/140‑1 (MQ=255)
                                                   gatgatAAGAAAAACCGCTCCAAGAATCATAATGATCTTAGGGAATTCCGTCATTTGGGGGAGCCTCCATTTGCAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGc                                                                                    <  1:3198551/140‑1 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGTATAAATCCGATTTGCa                                                                                   >  2:3054915/1‑140 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCa                                                                                   >  2:3356542/1‑140 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCa                                                                                   >  1:3918778/1‑140 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCa                                                                                   >  1:1765329/1‑140 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCa                                                                                   >  1:1206315/1‑140 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCa                                                                                   >  2:1814786/1‑140 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCa                                                                                   >  2:221730/1‑140 (MQ=255)
                                                    atgatAAGCAAAACAGCTCCAAGAATCATAATCATCTTAGGGAATTCAGTCATATCGGCGAGCCTCCATTTGGAAATGATGATAAACTGCCTGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCa                                                                                   >  1:2946909/1‑140 (MQ=255)
                                                     tgatAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCag                                                                                  <  1:804382/140‑1 (MQ=255)
                                                     tgatAAGCAAAACAGCACAAACAATCATAAGCATCTTCGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGAGAAACGGCCGGTGTCACGATCCGTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCag                                                                                  <  1:1187976/140‑1 (MQ=255)
                                                         aaGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCaggagg                                                                              >  2:2993820/1‑140 (MQ=255)
                                                         aaGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTGCTTTGAATAAATCCGATTTGCaggagg                                                                              >  1:4046886/1‑140 (MQ=255)
                                                          aGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGGGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGt                                                                             <  1:78242/140‑1 (MQ=255)
                                                          aGCAAAAAAGCTCCAATAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGTAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGt                                                                             <  1:2122043/140‑1 (MQ=255)
                                                           tcAAAACAGCTCCAAGAATCATTATGATCTTAGGGATTTCAGTAATTTCGGGGTGCCTCCATTTGGAATTGATTATAAACGGCCGGTGTCACGATCCTTCCACGCGGCGTTCTTTTAATAAATCCGATTTGCAGGAGGTa                                                                            <  2:798907/139‑1 (MQ=255)
                                                           gCAAAACAGCTCCAATAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTa                                                                            <  2:1395834/140‑1 (MQ=255)
                                                           gCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATCTCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTa                                                                            <  2:4244446/140‑1 (MQ=255)
                                                           ccAACACACCTCCACGAATCATAAGGATCTCAGGGCCTGCCGTCCTCGCGGGGAGCCGCCCTTTGGCCATGATGAGACACGGCCGGGGTCACGAGCCCGCCCCGCGGCCTTCTTTCAATAAATCCGATTTGCAGCAGGTa                                                                            <  2:414362/139‑1 (MQ=255)
                                                            cAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGTGCCTCCATTTGGAAATGATGATAAACGGCAGGTGTCACGATCCTTCCCCTCGGCGTTCTTTGACTAAATCCGATTTGCAGGAGGTAc                                                                           >  2:2424160/1‑140 (MQ=255)
                                                            cAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCAGGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTAc                                                                           >  2:2486900/1‑140 (MQ=255)
                                                             aaaaCCGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACg                                                                          <  2:1371347/140‑1 (MQ=255)
                                                             aaaaCCGCTCCAAGAATCATAATCATCTTAGGCAATTCCGTCATATCGGGGAGCCTCCATTTGTAAATTATGACACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACg                                                                          <  1:2308215/140‑1 (MQ=255)
                                                             aaaaCAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACg                                                                          <  1:1976624/140‑1 (MQ=255)
                                                                  cgcgccAAGAATCATCATGTTCTTAGGGATTTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCg                                                                     <  2:3478622/136‑1 (MQ=255)
                                                                    cTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTa                                                                   >  2:1177031/1‑140 (MQ=255)
                                                                     cccAAGAATCATAAGGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGGGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTac                                                                  <  2:2504997/139‑1 (MQ=255)
                                                                      ccAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTaca                                                                 <  1:4169531/140‑1 (MQ=255)
                                                                      ccAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTaca                                                                 <  1:4187796/140‑1 (MQ=255)
                                                                       caacactCATAATGATCTGAGGGAATCCAGTCATATCGGGGCGCCACAATTTGTCAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCATTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTacac                                                                <  1:382917/134‑1 (MQ=255)
                                                                       cAAGAATCATAATGATCTTAGGGAATTCAGTCTTATCGGGGCGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTacac                                                                <  1:1586651/140‑1 (MQ=255)
                                                                       cAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTacac                                                                <  2:3462952/140‑1 (MQ=255)
                                                                         aGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGt                                                              >  2:2935823/1‑140 (MQ=255)
                                                                            accATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGAGAAACGGCCGGGGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCtt                                                           <  2:2687233/138‑1 (MQ=255)
                                                                            aTCATAATGATCTTATGTAATTCAGTCATATGGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCtt                                                           <  2:1728122/140‑1 (MQ=255)
                                                                            aTCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCtt                                                           <  2:3161797/140‑1 (MQ=255)
                                                                               aTAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCaa                                                        <  1:2993820/140‑1 (MQ=255)
                                                                                     aTCTTAGGGACTTCAGTCATATCTGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCGCCGCGGCGTTCTTTGAATAAATCCGATTTGCTGGAGGTACGGTTCGTACACGTGTTCAATCGTAt                                                  >  2:3497526/1‑140 (MQ=255)
                                                                                     aTCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTAt                                                  >  2:3568500/1‑140 (MQ=255)
                                                                                      tCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATg                                                 <  2:499336/140‑1 (MQ=255)
                                                                                         tAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCgg                                              >  2:2238657/1‑140 (MQ=255)
                                                                                           ggtaaTTCCGTCATAGCGGGGAGCCTCCATTTGGAAATGATGATACACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGtt                                            <  1:3457679/137‑1 (MQ=255)
                                                                                           gggAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACg                                                                          <  2:3082846/110‑1 (MQ=255)
                                                                                                ttCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGttcttc                                        >  1:3411432/1‑139 (MQ=255)
                                                                                                ttCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGttcttc                                        <  2:3411432/139‑1 (MQ=255)
                                                                                                ttCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCg                                       >  2:2638791/1‑140 (MQ=255)
                                                                                                ttCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCg                                       >  2:869907/1‑140 (MQ=255)
                                                                                                ttCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCg                                       >  2:3126933/1‑140 (MQ=255)
                                                                                                ttCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCg                                       >  2:1267624/1‑140 (MQ=255)
                                                                                                ttCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCg                                       >  2:1029141/1‑140 (MQ=255)
                                                                                                        tatCGGGGAGCCTCCCTTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGt                               >  2:2758226/1‑140 (MQ=255)
                                                                                                        tatCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGt                               >  2:4188471/1‑140 (MQ=255)
                                                                                                          tCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTgg                             >  2:3885164/1‑140 (MQ=255)
                                                                                                           cGGGGAGACTCCTATTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGc                            <  2:380630/140‑1 (MQ=255)
                                                                                                               gAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACg                                                                          >  2:2959848/1‑90 (MQ=255)
                                                                                                               gAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGaa                        >  2:1087668/1‑140 (MQ=255)
                                                                                                                  ccTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATg                     <  2:3006707/140‑1 (MQ=255)
                                                                                                                  ccTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATg                     >  1:302181/1‑140 (MQ=255)
                                                                                                                  ccTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATg                     >  2:2052836/1‑140 (MQ=255)
                                                                                                                   cTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGc                            >  1:2219386/1‑132 (MQ=255)
                                                                                                                    tCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGt                   >  2:3443290/1‑140 (MQ=255)
                                                                                                                    tCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGt                   >  1:1360312/1‑140 (MQ=255)
                                                                                                                    tCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATAGTGGCTGAAATGGt                   >  2:3743882/1‑140 (MQ=255)
                                                                                                                           ggAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGTATCgaga            <  1:3087335/140‑1 (MQ=255)
                                                                                                                             aaaTGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGTATCGAGAcc          <  1:1002317/140‑1 (MQ=255)
                                                                                                                             aaaTGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGTATCGAGAcc          <  1:846709/140‑1 (MQ=255)
                                                                                                                                tgatgaTAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGTATCGAGACCCAc       >  2:1658034/1‑140 (MQ=255)
                                                                                                                                  atgatAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGTATCGAGACCCACCg     >  2:1581983/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
TTTCACAAAAATATCTCCCGGCATCTTGCCGACAAAATGCAATACTGCTCCGATGATAAGCAAAACAGCTCCAAGAATCATAATGATCTTAGGGAATTCAGTCATATCGGGGAGCCTCCATTTGGAAATGATGATAAACGGCCGGTGTCACGATCCTTCCCCGCGGCGTTCTTTGAATAAATCCGATTTGCAGGAGGTACGGTTCGTACACGTCTTCAATCGTATGCGGTTCTTCGCCAATCGTGGCTGAAATGGTATCGAGACCCACCGGGC  >  NZ_CP034943/2643883‑2644155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: