Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 135,117 G→T 11.0% intergenic (+164/‑74) EO946_RS00715 → / → rpsJ alpha/beta hydrolase/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943135,1170GT11.0% 646.7 / 13.1 309intergenic (+164/‑74)EO946_RS00715/rpsJalpha/beta hydrolase/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base G (151/111);  new base T (45/2);  total (196/113)
Fisher's exact test for biased strand distribution p-value = 4.22e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

ACCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCA  >  NZ_CP034943/134979‑135256
                                                                                                                                           |                                                                                                                                           
acCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            <  1:3544330/140‑1 (MQ=255)
  ctctataATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCa                                                                                                                                          <  1:4220967/137‑1 (MQ=255)
  cTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGGTGCTGACACACCCGGCCGCTTTGCCa                                                                                                                                          <  2:1692878/140‑1 (MQ=255)
    gTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTGTTGcctg                                                                                                                                         >  1:152596/1‑138 (MQ=255)
        aataaAAAAAGTGTGCAAACCATGCATATTTTAAATACGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGATGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCaa                                                                                                                                    <  1:4132916/140‑1 (MQ=255)
          taaAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAg                                                                                                                                  >  1:116408/1‑140 (MQ=255)
              aaaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGgtgt                                                                                                                              <  1:271608/140‑1 (MQ=255)
               aaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTTATACATACGTCTATTTTCTTTATAATGGTGTATGTTGTTCTTTGAATGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGtt                                                                                                                             <  1:36825/140‑1 (MQ=255)
               aaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGtt                                                                                                                             <  2:38303/140‑1 (MQ=255)
               aaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGATGACACACCCGGCCGCTTTTCCATGGGGAAGGGGtt                                                                                                                             >  1:430342/1‑140 (MQ=255)
                    gtgCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAATGTGTTGAGGt                                                                                                                        >  1:2571390/1‑140 (MQ=255)
                             aTGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCCTGGCAAAGGTGGTGAGGtttttataac                                                                                                               >  1:3313282/1‑135 (MQ=255)
                             aTGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAACGTGTTCAGGtttttgtaac                                                                                                               >  1:2420039/1‑135 (MQ=255)
                             aTGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGGGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCGATGGCAAAGGTGTTCTGGTTTTTCTCAc                                                                                                               >  1:3903453/1‑140 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:1284214/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  2:711502/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  2:3881352/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:3734254/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTAGTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  2:2615498/140‑1 (MQ=255)
                                     tttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCAAGGTGTTGAGGTTTTTATCACGGAGAACt                                                                                                       >  1:1458765/1‑140 (MQ=255)
                                             aGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACt                                                                                               <  1:2452119/140‑1 (MQ=255)
                                             aGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACt                                                                                               <  1:1791534/140‑1 (MQ=255)
                                              gTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACtt                                                                                              >  2:891763/1‑140 (MQ=255)
                                                         tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCAAAGTTGTTAATGTTTTTATAAAGGAGAACAGTCTAAAATTATGTAGGCGa                                                                                   >  1:1012341/1‑140 (MQ=255)
                                                               tATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            >  2:1087531/1‑77 (MQ=255)
                                                               tATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:2041004/112‑1 (MQ=255)
                                                               tATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         >  2:2041004/1‑112 (MQ=255)
                                                               tATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTCCATGGCAAAGGTGTTCAGGTTTTTATCAAGGAGAACAGTATAACTTGATGTAGGCGAAAacga                                                                             >  1:3073143/1‑140 (MQ=255)
                                                                 ttttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAAGGCAAATGTTTTGAGGTTTTTATCACCGAGAACTGTGAAAAAGGATGTAGGCGCAAaggagg                                                                           >  1:2253097/1‑140 (MQ=255)
                                                                 ttttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTCCATGGCAAAGGTGTTCAGGTTTTTATCACCGAGAACTGTCTAACTTGATGTAGTCGAAAaagagg                                                                           >  1:3629308/1‑140 (MQ=255)
                                                                      tggataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACAAACCCGGCCGCTTTTCCATGACAAAGGTGTTGAGGTTTTTCTAACGGAGAACTGTCTAAATTGATTTATGCGAAAAAGATGGaaaa                                                                      >  2:3550036/4‑140 (MQ=255)
                                                                      tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCAAGGCCAAGGTTTTCAGGTTTTTATAAAGGAGAACATTCTAACATTATGTTTGAGAAAAGGAGGGaaaa                                                                      >  1:3775342/1‑140 (MQ=255)
                                                                      tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTTGTAAGGGTTTTATAACCGATAACAGTCTAACTTGATGTAGGCGGAAAAGATGGaaaa                                                                      >  1:2135299/1‑140 (MQ=255)
                                                                      tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGTTGTAGGCGAAAATTAGGGaaaa                                                                      >  2:1966164/1‑140 (MQ=255)
                                                                      tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGaaaa                                                                      >  2:4002424/1‑140 (MQ=255)
                                                                      tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTAACGGAGAACTGTCTAACTTGATGTAGGCGACAAGGAGGGacca                                                                      >  2:3369576/1‑137 (MQ=255)
                                                                      tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTATCACCGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGaaaa                                                                      >  2:2491264/1‑140 (MQ=255)
                                                                             tGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAATGAGGGAAAATAATGGc                                                               >  2:2875724/1‑140 (MQ=255)
                                                                             tGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGc                                                               >  2:4058106/1‑140 (MQ=255)
                                                                                tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGTTTTTTCTAACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaa                                                            >  2:1015495/1‑140 (MQ=255)
                                                                                tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTATGCGAAAAGGAGGGAAAATAATTGcaaa                                                            >  2:465330/1‑140 (MQ=255)
                                                                                tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaa                                                            >  2:2066532/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCTCTTTGCCAAGGCAAAGGTGTTCAGGTTTTTATAACAGAGCAATCTATACATTGATGTAAGCGAAAAGCAGGGCAAATAAAGTcaaaaa                                                          >  1:1066704/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACTTGATGTATGCGAAAAGGAGGGAAAAAAAAGGcaaaaa                                                          >  1:3104115/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATTGCAAAGGGGTTAAGGTTTTTGTGAAGGAGAAATGTCTAACATTATGTAGGCGAAAATGAGGGAAAATAATTGgaaaaa                                                          >  1:1716808/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTGAGGTTTTTGTAAACGATAAATGTATAACTTTATGTAGGCGAAAAAGGGGGAAAAAAAAGGcaaaaa                                                          >  1:1812817/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTGAGGTTTTTCTGACGGAGAACTGTGTAACTTGATTTAGGCGAAAAGGGGGGAAAAAAATGGcaaaac                                                          >  1:1178588/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaaac                                                          >  2:5634/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTCATGTATGAGAAAAGGAGGGAAAATAATGGcaaaac                                                          >  2:3752008/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAGGGCAAAGGTGTTCATGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGcaaaac                                                          >  1:3239312/1‑140 (MQ=255)
                                                                                  gaTGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCGCTGGGAAACCTGTTCAGGTTTTTGTCACGGCGAACTGTCTAAAGTTCGGTAGGCGAAATGGTGGGTAAATAATGGcaaaag                                                          >  1:2270650/2‑139 (MQ=255)
                                                                                       tGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCAATGTGTTCAGGTTTTTATCACGGAGTACTGTCTAAAATGATGTAGGCGAAAAGGAGGGAAAAAAATGGCaaaaaaaaaa                                                     >  1:4103604/1‑140 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCCAAGGTGTTCCGGTTTTTTTCACGGCGTAATGTCTAAATTGATGTTGGCGACAAGGGGGGGAAATAATTGCAAAACaaaaaaa                                                   >  1:265426/1‑139 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATTGCAAAGGTGTTCAGGTTTTTGTCACGGAGAACAGTCTAAATTGAGTTAGGCGAAAAGGAGGGTAAAAAATGGCAaaaaaaaaaaa                                                   >  1:1934549/1‑139 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAat                                                   >  2:809896/1‑140 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAAAAAAAAat                                                   >  2:3639100/1‑140 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGGCATGGCAAAGGTGGTGAGGTTTTTGTCACCGAGGAAGGTCTAAAGTGATGTATGCGCAAAGGGGGGCAAAAAATGGCAAAACAAAAAat                                                   >  1:1424788/1‑140 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGCCATGGCAAAGGTGTTCAGGTTTTTGTCACGGAGAAAATTCTAACATGATGTAGGCGAAAATGAGGGAAAATAATTGCAAAAAAAAAAat                                                   >  1:3048379/1‑140 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGCCATGGCAAAAGTGTTTAGCTTTTTATCACGGACAACAGTCTAACATGCTGTAGGCGGAAAGGAGGGAAAAAAAAGGCAAAACAAAAAat                                                   >  1:4038361/1‑140 (MQ=255)
                                                                                            tttGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCCAAGGGGGTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGGCAATAATGGCAAAACAAAAAattcg                                                >  1:1561715/1‑140 (MQ=255)
                                                                                            tttGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  2:87383/73‑1 (MQ=255)
                                                                                            tttGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   >  1:3993488/1‑73 (MQ=255)
                                                                                              tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCGAAGGTGTTAAGGTTTTTATCACGGAGAACATTATAAATTTATTTAGGCGAAAAGGAGGGCAAATAATAGCAAAAAAAAAAataagta                                              >  1:567289/1‑135 (MQ=255)
                                                                                              tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCCGGGCAAAGGTGTTGAGGTTTTTGTGACGGAGAACAGTCTAAAATGTTGTAGGCGAAAAGGAGGGAAAAAAAAGGCAAAAAAAAAAataagta                                              >  1:1212874/1‑135 (MQ=255)
                                                                                              tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCAAAGGTGTTGAGGTTTTTATAAAGGGGAACAGTGTAACTTGATGTAGGCGAAAAGGAGGGAAAAAAAATGCAAAAAAAAAAaatagta                                              >  1:315821/1‑140 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGCAAAGGTGTTGAGGTTTTTGTGCCGGGGGACTTTGTAACTTGTTGTAGGGGGAAAGGAGGGAAAATAATTGCAAAAAAAAAAAATAGTATTGGtt                                        >  1:2826269/1‑140 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGCGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGtt                                        >  2:2910860/1‑140 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTTATTTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGtt                                        >  2:1156957/1‑140 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGtt                                        >  2:342556/1‑140 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCACGTTTTTCTCACGGAGAACTGTCTAACTTTATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattagtattcggt                                        >  2:630020/1‑138 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAAGGGTTCAGGTTTTTATGACGCAGAACAGTATAACGAGATTTGGGGGAAAAGGACGGAAAAAAAAGTCAAAAAAAAAAAATCTTATTCGtt                                        >  1:133026/1‑140 (MQ=255)
                                                                                                       tgtaGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGa                                     <  2:3307693/137‑1 (MQ=255)
                                                                                                       tGAAGGGAGAGGTTGAGGACAACCCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGCCAAAACAAAAAATTCGTATTCGTTTGa                                     <  2:1444787/140‑1 (MQ=255)
                                                                                                            tGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                <  1:4058106/140‑1 (MQ=255)
                                                                                                            tGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                <  1:795845/140‑1 (MQ=255)
                                                                                                            caggagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                <  2:2999575/137‑1 (MQ=255)
                                                                                                                 ggctGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAAGTCTCTCACTTGATGTAGGCGAAAAGGACGGAAAATAATGGCAAACCAAAAAATTCGTATTCGTTTGAAAGCATATGa                           <  1:809896/137‑1 (MQ=255)
                                                                                                                 ggTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGa                           <  2:922626/140‑1 (MQ=255)
                                                                                                                 ggTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGa                           <  2:3095136/140‑1 (MQ=255)
                                                                                                                     gCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTTACTTGATTTAGGCGAAAAGGAGGGACAATAATGGCAAAAAAAAAAAATAGTATTGGTTTGTAATCATATGATAAt                       >  1:2180647/1‑140 (MQ=255)
                                                                                                                     gCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                >  2:3452579/1‑131 (MQ=255)
                                                                                                                     gCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                <  1:3452579/131‑1 (MQ=255)
                                                                                                                     gCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACATGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTACTAGTCGTTTGAAACCAAATGATCaa                       >  2:1336372/1‑139 (MQ=255)
                                                                                                                      cTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATa                      <  2:1256499/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  2:315341/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  1:941443/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  2:2764726/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  2:2325155/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  1:184723/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTATTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAACGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  1:2334055/140‑1 (MQ=255)
                                                                                                                         acacacCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGaa                   <  2:1812817/140‑1 (MQ=255)
                                                                                                                          cacacCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACg                                                                                                              >  2:1495176/1‑48 (MQ=255)
                                                                                                                          cacacCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAAt                  >  2:4124318/1‑140 (MQ=255)
                                                                                                                            cacCCGGCCGCGTTGCCATGGCCAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGCAAAGGAGGGAAAATAATTGCAAAACAAAAAAATCGTATGCGTTTGAAAGCCTATGATCATAGAAtac                >  2:1742946/1‑138 (MQ=255)
                                                                                                                             acCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATc                 <  1:3370918/138‑1 (MQ=255)
                                                                                                                                cGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGa            <  1:918568/140‑1 (MQ=255)
                                                                                                                                cGGCCGCTGTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATCGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTGGa            <  2:418698/140‑1 (MQ=255)
                                                                                                                                   ccGCTTTGCCCTGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCGAACGTGATGTAGGCGCAAAGGAGGGAACATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCa         >  2:2226044/1‑140 (MQ=255)
                                                                                                                                     gCTTTGCCATGGCAAAGGTGTTCATGTTTTTCTCACGGAGAACTGTCTAACTTTATGTAGGCGAAAAGGAGTGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTTAAATTTTATGATCATAGAATCCTTTAtatat       <  2:230615/140‑5 (MQ=255)
                                                                                                                                           gCCATCGCAAAGGTGATCAGGATTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGCACAGGAGGGCAAAAAATGGCAAAACAAAACATTCGTAGTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCa  <  1:2944702/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCA  >  NZ_CP034943/134979‑135256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: