Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,649,600 T→C 6.6% intergenic (+3/+43) EO946_RS13705 → / ← EO946_RS13710 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,649,6000TC 6.6% 402.6 / 13.3 124intergenic (+3/+43)EO946_RS13705/EO946_RS13710hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (58/57);  new base C (4/4);  total (63/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

Only 100 of 134 total aligned reads displayed.

GCTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCTTCTGTGCCT  >  NZ_CP034943/2649463‑2649733
                                                                                                                                         |                                                                                                                                      
gCTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCttaaattaa                                                                                                                                      >  1:3848967/1‑140 (MQ=255)
gCTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCttaaattaa                                                                                                                                      >  1:3687876/1‑140 (MQ=255)
gCTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCttaaattaa                                                                                                                                      >  1:765850/1‑140 (MQ=255)
 cTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCttaaattaaa                                                                                                                                     <  2:3555143/140‑1 (MQ=255)
 cTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCttaaattaaa                                                                                                                                     >  1:2298423/1‑140 (MQ=255)
 cTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCttaaattaaa                                                                                                                                     >  1:172620/1‑140 (MQ=255)
  ttAACACGCACTCTACAAAAAAACTATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCTTTTTTATATAAGAAAGATTGGTACCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTaaaa                                                                                                                                    <  1:620739/140‑1 (MQ=255)
      cACGCACACTACAGAAAACCGATAGTCCCTAACAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATACGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGc                                                                                                                                <  2:3337853/140‑1 (MQ=255)
           aCTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAATACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAATTAGGCAGcca                                                                                                                           >  2:3004347/1‑139 (MQ=255)
           aCTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAATCATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCg                                                                                                                           >  1:546135/1‑140 (MQ=255)
           aCTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCg                                                                                                                           >  1:149720/1‑140 (MQ=255)
           aCTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCg                                                                                                                           >  2:1404268/1‑140 (MQ=255)
           aCTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCg                                                                                                                           >  1:454160/1‑140 (MQ=255)
           aCTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTACCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAATTTAAAAAGTCAGCCg                                                                                                                           >  2:2424828/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGcaca                                                                                                                       >  2:1550936/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGcaca                                                                                                                       >  2:538058/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGcaca                                                                                                                       >  2:2676884/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGcaca                                                                                                                       >  2:3165905/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGcaca                                                                                                                       >  2:1746894/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCAGCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGcaca                                                                                                                       >  2:15696/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCCAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTTCAAAAGCATTTCGCACAGCGGAAAAGAAAATATTCTTAAATCAAAAAGGCAGCCGccca                                                                                                                       >  2:415813/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCCAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTTCAAAAGCATTTCGCACAGCGGAAAAGAAAATATTCTTAAATCAAAAAGGCAGCCGcaca                                                                                                                       >  2:2050188/1‑140 (MQ=255)
               tACAAAAAACCGATATTCCCCAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTTCAAAAGCATTTCGCACAGCGGAAAAGAAAATATTCTTAAATCAAAAAGGCAGCCGcaca                                                                                                                       >  2:416018/1‑140 (MQ=255)
                       aCCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTATAAAGGCAGCCGCACAAGCGGCTg                                                                                                               >  2:3169204/1‑140 (MQ=255)
                       aCCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAATGGGAGCGGCACATGCGGCTg                                                                                                               >  2:4049050/1‑140 (MQ=255)
                       aCCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTg                                                                                                               >  1:4241280/1‑140 (MQ=255)
                       aCCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTg                                                                                                               >  1:4057826/1‑140 (MQ=255)
                       aCCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTg                                                                                                               >  2:2066294/1‑140 (MQ=255)
                         cGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAATGGCAGCCGCACAAGCGGCTGcc                                                                                                             >  2:345680/1‑140 (MQ=255)
                         cGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGcc                                                                                                             >  1:1049096/1‑140 (MQ=255)
                         cGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGcc                                                                                                             >  1:1896782/1‑140 (MQ=255)
                           atatTCCCTAAAAATATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCtt                                                                                                           <  2:3097546/140‑1 (MQ=255)
                                cccTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTc                                                                                                      >  2:2848160/1‑140 (MQ=255)
                                 ccTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAATAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCa                                                                                                     <  1:637808/140‑1 (MQ=255)
                                   tAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCata                                                                                                   >  1:418274/1‑140 (MQ=255)
                                   tAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCata                                                                                                   >  2:1692361/1‑140 (MQ=255)
                                          aTTCCGGGTTTTTGGCTGCAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAAGATTTCGCACAGCGGAAAAGTAAATATTCTTAAATTAAAAATGCAGCCGCACAAGCGGCTGCCTGCGTTCATATAttttt                                                                                           >  2:72606/1‑140 (MQ=255)
                                           ttCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCAGAGCGGAAAAGAAAATATTCTTACATTAAAAAGGCAGCCGCACAAGAGGCTGCCTTCGGTCATATATTTTTa                                                                                          >  1:1903686/1‑140 (MQ=255)
                                            tCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATAtttttcc                                                                                         >  1:2272850/1‑138 (MQ=255)
                                                            gagaTCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGaaa                                                                         <  2:2195353/140‑1 (MQ=255)
                                                            gagaTCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGaaa                                                                         <  2:2150976/140‑1 (MQ=255)
                                                            gagaTCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGaaa                                                                         <  2:2070383/140‑1 (MQ=255)
                                                                      cATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCAt                                                               <  2:949482/140‑1 (MQ=255)
                                                                         ttttATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAACTCAGTTTCATTTg                                                            >  2:684638/1‑140 (MQ=255)
                                                                           ttATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGtt                                                          <  2:454160/140‑1 (MQ=255)
                                                                           ttATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGtt                                                          <  2:546135/140‑1 (MQ=255)
                                                                             atataAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAACTCAGTTTCATTTGTTCa                                                        >  1:4183377/1‑140 (MQ=255)
                                                                                  agaaagaTTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGa                                                   <  1:683469/140‑1 (MQ=255)
                                                                                  agaaagaTTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGa                                                   <  2:3848967/140‑1 (MQ=255)
                                                                                  agaaagaTTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGa                                                   <  2:3078055/140‑1 (MQ=255)
                                                                                  agaaagaTTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGa                                                   <  2:149720/140‑1 (MQ=255)
                                                                                        aTTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCtt                                             >  1:1055187/1‑140 (MQ=255)
                                                                                              tCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCg                                       >  2:2951250/1‑140 (MQ=255)
                                                                                               ccAAAAACATTTCGCACAGCGGAAAATAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGa                                      <  1:2468391/140‑1 (MQ=255)
                                                                                               ccAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGa                                      <  1:3423931/140‑1 (MQ=255)
                                                                                                 aaaaaCATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATa                                    >  2:907555/1‑140 (MQ=255)
                                                                                                  aaaaCATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAg                                   <  2:637674/140‑1 (MQ=255)
                                                                                                       aTTTGGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAATGCAGCCGCACAAGCGGATGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  1:2883085/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCg                                                                                                         >  2:3456442/1‑66 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCg                                                                                                         <  1:3456442/66‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCg                                                                                                         <  1:2094522/66‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  2:4057826/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  2:4241280/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  1:3165905/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  1:2676884/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  1:1550936/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  1:2848160/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGGAAAAGAAAATATTCGTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  1:2133758/140‑1 (MQ=255)
                                                                                                       aTTTCGCACAGCGCAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  1:3714832/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATCTTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  1:988796/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  2:4229404/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  1:1900141/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  1:15640/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  2:1199625/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  2:3376317/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  2:335174/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  2:3377673/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATCAAAAAGGCAGCCGCACAAGCGGCTGCCTTTATCATGTATTTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  1:416018/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATCAAAAAGGCAGCCGCACAAGCGGCTGCCTTTATCATGTATTTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  1:2050188/140‑1 (MQ=255)
                                                                                                         ttCGCACAGCGGAAAAGAAAATATTCTTAAATCAAAAAGGCAGCCGCACAAGCGGCTGCCTTTATCATGTATTTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTca                            <  1:3377449/140‑1 (MQ=255)
                                                                                                          tCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              >  1:61957/1‑137 (MQ=255)
                                                                                                          tCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCctct                              <  2:61957/137‑1 (MQ=255)
                                                                                                           cGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTcaca                          >  2:469733/1‑140 (MQ=255)
                                                                                                           cGCACAGCGGAAAAGAAAATAGTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCCGGCGACAGCTTAATTCGATAGCCTCTcaca                          <  1:1730024/140‑1 (MQ=255)
                                                                                                                 gCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTaaa                    >  1:482829/1‑140 (MQ=255)
                                                                                                                  cGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAAt                   >  2:951088/1‑140 (MQ=255)
                                                                                                                  cGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAAt                   >  2:4132123/1‑140 (MQ=255)
                                                                                                                  attaaaagaaaaTATTCTTAAATAAAAAAAAAAACCCCACAAGCTGCTGCGATCGTTCATATATTTTTACTTTCTGACTTAATAAAAAAGAATCATATATTCAGCCGACAGATTAATTCGATAGCCTCTCACAGAAAAAt                   >  2:1432101/4‑140 (MQ=255)
                                                                                                                      aaagaaaaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGAc               <  1:1628892/140‑1 (MQ=255)
                                                                                                                      aaagaaaaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGAc               <  1:3870253/140‑1 (MQ=255)
                                                                                                                      aaagaaaaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGAc               <  1:1329492/140‑1 (MQ=255)
                                                                                                                      aaagaaaaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGAc               <  1:3094731/140‑1 (MQ=255)
                                                                                                                           aaaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCtt          <  2:3687876/140‑1 (MQ=255)
                                                                                                                           aaaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCtt          <  2:816231/140‑1 (MQ=255)
                                                                                                                            aaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATCTATTTTTACTTTCTGACGGCAGTAATCAGTTTCATTTGTTAAGGCGACAGCTTAAGTCGATAGCCTAACACATTTAAATAGACCGCTTc         >  1:3947909/1‑140 (MQ=255)
                                                                                                                            aaTATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAAACAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCTTc         >  2:3626059/1‑140 (MQ=255)
                                                                                                                                ttCTTAAATTAAAAAGGCAGCCGCACAAGGGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCTTCtgtg     <  2:1080101/140‑1 (MQ=255)
                                                                                                                                 tCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCTTCTGTGc    >  2:2216983/1‑140 (MQ=255)
                                                                                                                                   ttaaattaaaAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCTTCTGTGCCt  >  1:3077383/1‑140 (MQ=255)
                                                                                                                                   ttaaattaaaAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCTTCTGTGCCt  >  2:1849442/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
GCTTATCACGCACTCTACAAAAAACCGATATTCCCTAAAAAGATTCCGGGTTTTTGGCTGGAGATCATCGCATTTTTATATAAGAAAGATTGGTTCCAAAAACATTTCGCACAGCGGAAAAGAAAATATTCTTAAATTAAAAAGGCAGCCGCACAAGCGGCTGCCTTCGTTCATATATTTTTACTTTCTGACGGCAGAAATCAGTTTCATTTGTTCAGGCGACAGCTTAATTCGATAGCCTCTCACAGTTAAATAGACCGCTTCTGTGCCT  >  NZ_CP034943/2649463‑2649733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: