Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,649,964 C→A 6.0% intergenic (‑199/‑75) EO946_RS13710 ← / → EO946_RS13715 hypothetical protein/alanine:cation symporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,649,9640CA 6.0% 618.9 / 2.5 225intergenic (‑199/‑75)EO946_RS13710/EO946_RS13715hypothetical protein/alanine:cation symporter family protein
Reads supporting (aligned to +/- strand):  ref base C (89/111);  new base A (22/2);  total (112/113)
Fisher's exact test for biased strand distribution p-value = 6.21e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 227 total aligned reads displayed.

TTTAAGGAAATGTAAAGATAGTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTACCCCCGTCATTTATATTTTA  >  NZ_CP034943/2649828‑2650101
                                                                                                                                        |                                                                                                                                         
tttAAGGAAATGTAAAGATAGTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGAt                                                                                                                                        <  2:1294990/140‑1 (MQ=255)
         aTGTAAAGATAGTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAAt                                                                                                                               >  2:554836/1‑140 (MQ=255)
                 aTAGTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTGTTATTGATAATTACCGGTCATATAATGTGACAAAGCGATTGGAGACATAGGTCGAt                                                                                                                       >  2:2188674/1‑140 (MQ=255)
                  tAGTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGAtt                                                                                                                      <  1:3004404/140‑1 (MQ=255)
                    gTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTACTTCCTGTTGTTATTTTTAATTAACGGTCATATAATTTTACAAATAGATTTTAGAAATAGTTAGAtttt                                                                                                                    >  1:631452/1‑138 (MQ=255)
                    gTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTGTTTTCTTTTTTCCTTGGGCTTCTTGTTTATAATTAACGGTCATATAATTTGACAAATAGAGTGTTGAAATATTTGGATTat                                                                                                                    >  1:3212025/1‑139 (MQ=255)
                    gTATAAACCTCATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTATTTTTTACATCCTCTTCGTTTTGATAATTAACCGTCATATAATTTGAAAAATAGATTGTAGAAATAGTTCGATTAg                                                                                                                    >  1:4248584/1‑140 (MQ=255)
                     tataAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGTTAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGc                                                                                                                   >  2:612120/1‑140 (MQ=255)
                     tataAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGAAAATTACCGGTCATATAATGTGAGAAATCGATTGTATAAAAAGTTCGATTAGc                                                                                                                   >  2:3025496/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTCCGTGCTCTTCTGAGTGTTAATTACCGGTCATATTATGTGTCAAATCGGTTGGAGAAATAGTGCGGTTAGCCCAAAATCCt                                                                                                         >  1:2021559/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTACTTCCTCTTCTTATTGATAATTAACGGTCATATAATGTGACAAATAGATTTTAGAAATAGTTCGATTATCCCAAAATACt                                                                                                         >  1:1649953/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTACTTCCTCTTCTTATTGATAATTAACGGTCATATAATGTGACAAAAAGATTGTAGAAATATTTCGATTATCCCaaaaaact                                                                                                         >  1:4104618/1‑136 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTACTTCCTCTTCTTATTGATAAATACCGGTCATATAATGTGACAAAACGATTTTAGAAATAGTTCGATTATCCCAAAATCCt                                                                                                         >  1:3947571/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTGTTATTGATAAATAACGGTCATATAATGTGAAAAATACATTTTATAAATAGTTAGATTAGCCCaaaaaaca                                                                                                         >  1:1893775/1‑136 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCCTATAATGTGACAAAACGAGTGTAGAAATAGTTCGATTAGCCCAAAATccg                                                                                                         >  1:1540594/1‑139 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCt                                                                                                         >  2:2461236/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCt                                                                                                         >  2:1650198/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCt                                                                                                         >  2:4067619/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCt                                                                                                         >  2:720188/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCt                                                                                                         >  2:739146/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCt                                                                                                         >  2:2135687/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAAGGTTACAAATCCATTGTAGAAATATTTCGATTATCCCAAAATGCt                                                                                                         >  1:4043579/1‑140 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTACTTCCTCTTGTTATTGATAATTAACGGTCATATAAATTGACAAATAGATTGTAGAAATAGTTCGATTATCCCAAAATccg                                                                                                         >  1:1850389/1‑139 (MQ=255)
                               atatGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTACTTCCTCTTCGTATTGATAAATAACGGTAATATAATGTTACAAATAGATTGTAGAAATATTTAGTTTATCCCAAAATCCt                                                                                                         >  1:2504645/1‑140 (MQ=255)
                                 aggtggtgAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCttt                                                                                                       <  1:1602288/138‑1 (MQ=255)
                                    tggtgAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTta                                                                                                    >  2:2278817/1‑140 (MQ=255)
                                      gtgAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTGCTTACTGTTCTTATTGATAATTACCGGTCATATAATGTTACAAAAAGATTTTAGAAATATTTCGATTAGCCCAAAATACTTTTtata                                                                                                  >  1:3889866/1‑140 (MQ=255)
                                      gtgAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTCCTTCCGCTTCTTATTTTTAATTAACGGTCAGATAATGTGACAAATCGATTGTATAAATATTTCGATTAGCCCAAAATACTTTTtata                                                                                                  >  1:3550635/1‑140 (MQ=255)
                                      gtgAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTGTTTTTTACTTGCGCTTGTTATTGTTAATTAACGGTCATATAATTTGACAAATAGATTTTAGAAATAGTTCGATTATCCCAAAATGCTTTTtata                                                                                                  >  1:3606585/1‑140 (MQ=255)
                                      gtgAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTACTCTTCTTATTGATAATTACCGGTCATAAAATGTGACAAATCGATTGTATAAATAGTTCGATTAGCCCAAAATACTTTTtata                                                                                                  >  2:1146369/1‑140 (MQ=255)
                                              tAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTACGCTTCTTATTGGTAATTTCCGGTCATATAATGTGTCAAATCGATTGTTGTAATAGTTCGATTTGCCCAAAATCCTTTTTATACATGTAtt                                                                                          >  1:2062840/1‑140 (MQ=255)
                                                   caacGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCGGGTATTATGGGTAAATAACGGTGCTATAATTTGTCAAAAACATTGTTTAAATAGTTCGATTAGCCCCAAAACCTATTTATACATGTATTCGaaa                                                                                     >  1:2744007/1‑140 (MQ=255)
                                                    aacGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAAt                                                                                    <  2:26910/140‑1 (MQ=255)
                                                    aacGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAAt                                                                                    <  2:145429/140‑1 (MQ=255)
                                                      cGCAGCGTAATCAATACGCGGCGTGTTTTGTTTTTTGCTTGCGCGTCTTATTGTTAATTACCGGTGATATAATGTGACAAATCGATTGTAGAAATAGTTGGATTATCCCAAAATCCTTTTTTTACATGTATTGGAAATGa                                                                                  >  1:709015/1‑140 (MQ=255)
                                                         aGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGt                                                                               <  1:952915/140‑1 (MQ=255)
                                                           cGTAATCAATACGCGGCGTTTTTTATTTTTTGCTTCCGGTTGGTTTTGATGAATACCGGGCATATAATGTGACAAAAAGATTGTATAAATAGTTCGCTTATCCCAAAATAGATTTTATACAGGTGTTCGACATGCAGTgt                                                                             >  1:3194221/1‑139 (MQ=255)
                                                               aTCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTTACAAAACGAGTGTAGAAATAGGGCGATTATCCCAAAATCCTTTTTATACATGTATTAGAAATGAAGTgaggcg                                                                         >  1:963838/1‑138 (MQ=255)
                                                                tCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATGGATTGTAGTAATAGTTCGATTAGCCCAAAATCGTTTTTATAGCTGTATTGGAAAGGAAGTGaggcgg                                                                        >  2:2810306/1‑140 (MQ=255)
                                                                 cAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAggagga                                                                       <  2:2306267/140‑1 (MQ=255)
                                                                 cAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAggagga                                                                       <  2:483052/140‑1 (MQ=255)
                                                                     aCGCGGCGTTTTTTCTTTTTTCCTTCCTCTTGTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCt                                                                   >  2:152773/1‑140 (MQ=255)
                                                                     aCGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGAAAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCt                                                                   >  2:1706501/1‑140 (MQ=255)
                                                                     aCGCGGCGTTTTTTCTTTTTTACTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAAAAGATTGTAGAAAAAGTTAGATTATACCAAAATCCTTTTTATACAAGTATTAGAAATGAAGTGAGGAGGAAGCt                                                                   >  2:1792540/1‑140 (MQ=255)
                                                                     aCGCGGCGTGTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATAAATGTATTCGAAATGAAGTGAGGAGGAAGCt                                                                   >  2:3720673/1‑140 (MQ=255)
                                                                                        ttccttccTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGc                                                <  1:557788/140‑1 (MQ=255)
                                                                                                 cttcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTaaa                                       <  1:385263/140‑1 (MQ=255)
                                                                                                   tcttATTTATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  2:659855/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  2:1987275/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  2:1893775/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  2:2842221/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  2:1761563/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  2:219665/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:739146/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:1105384/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:1134895/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:1572531/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:1710806/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:2135687/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:2185320/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:2419054/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:2461236/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:2816529/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:3530456/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:4067619/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:934439/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTAGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:519946/140‑1 (MQ=255)
                                                                                                   tcttATTGATAATTACCGGTCATATAATGTGACAAATCGAGTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:276481/140‑1 (MQ=255)
                                                                                                   tctgaatGATAATTACCGGTCATATAATGTGACAAATCGAGTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCGTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:3466720/134‑1 (MQ=255)
                                                                                                   tctgATTGATAATTACCGGTCAGATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:731229/140‑1 (MQ=255)
                                                                                                   tcgtATTGATAATGACCGGCCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     <  1:2259929/137‑1 (MQ=255)
                                                                                                   tattATTGATAATTACCGGTCATATAATTTGACAAATAGATTGTAGAAATAGTTCGATTATCCCTAAATCCTTTTTTTACATGTATTATAAATGTAGTTAGGAGGAAGGTTTATTTTAGATTTTGTTGATAGttttatag                                     <  2:882185/138‑6 (MQ=255)
                                                                                                    cttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgc                                    <  1:3346436/140‑1 (MQ=255)
                                                                                                    cttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgc                                    <  1:3477889/140‑1 (MQ=255)
                                                                                                    cttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgc                                    <  1:818198/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  1:4121605/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  2:4248584/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  2:4104618/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  1:2876622/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  2:2504645/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTCATATAATGTGACAAATCGATGGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  2:1864171/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTCAGATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  1:2222791/140‑1 (MQ=255)
                                                                                                     ttATTGATAATTACCGGTAATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  2:2903695/140‑1 (MQ=255)
                                                                                                     ctATTGATAATTACCGGTCATCTAATGTGACAACTCGATGGGAGAAATAGTTCGATTAGCCCACAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcgcg                                   <  1:3576126/139‑1 (MQ=255)
                                                                                                       aTTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGt                                 >  1:1007194/1‑140 (MQ=255)
                                                                                                       aTTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCGAGTTTACACGCGGt                                 >  2:3113887/1‑140 (MQ=255)
                                                                                                       aTTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATAAGCCCAAAATCCTTTTTATACATGTATTCTAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGt                                 >  2:3908029/1‑140 (MQ=255)
                                                                                                          gATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAACTAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCtt                              >  2:3152250/1‑140 (MQ=255)
                                                                                                           aTAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGc                                                >  2:2172265/1‑121 (MQ=255)
                                                                                                           aTAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGc                                                <  1:2172265/121‑1 (MQ=255)
                                                                                                                     gTCATATAATGTGACAACTCGATTGTAGAAATAGTTCGATTATCCCAAAATCCTATTTATACATGTATTCGAAAGGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACCCGGTCTAATGTCGTAccc                   >  1:3770850/1‑140 (MQ=255)
                                                                                                                     gTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTAccc                   >  2:3685964/1‑140 (MQ=255)
                                                                                                                     gTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTAccc                   >  2:3551372/1‑140 (MQ=255)
                                                                                                                       cATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTAccccc                 >  1:2335817/1‑140 (MQ=255)
                                                                                                                          ataATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTACCCCCGTc              >  2:441124/1‑140 (MQ=255)
                                                                                                                                tgACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAAcg                                     >  1:1767858/1‑111 (MQ=255)
                                                                                                                                tgACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTACCCCCGTCATTtat        >  2:422092/1‑140 (MQ=255)
                                                                                                                                tgACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTACCCCCGTCATTtat        >  2:601469/1‑140 (MQ=255)
                                                                                                                                tgACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTACCCCCGTCATTtat        >  2:3417105/1‑140 (MQ=255)
                                                                                                                                tgACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTACCCCCGTCATTtat        >  1:140228/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TTTAAGGAAATGTAAAGATAGTATAAACCTGATATGTGGTGAAATTTAGAACAACGCAGCGTAATCAATACGCGGCGTTTTTTCTTTTTTCCTTCCTCTTCTTATTGATAATTACCGGTCATATAATGTGACAAATCGATTGTAGAAATAGTTCGATTAGCCCAAAATCCTTTTTATACATGTATTCGAAATGAAGTGAGGAGGAAGCTTGATGGCAGATTTTGTTGCTAGTTTAAACGCGGTCTTATGGAGTACCCCCGTCATTTATATTTTA  >  NZ_CP034943/2649828‑2650101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: