Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,680,491 A→C 8.4% F387V (TTC→GTC)  EO946_RS13875 ← LysM peptidoglycan‑binding domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,680,4910AC 8.4% 442.7 / 2.6 193F387V (TTC→GTC) EO946_RS13875LysM peptidoglycan‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (90/69);  new base C (8/17);  total (100/93)
Fisher's exact test for biased strand distribution p-value = 3.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

CTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTCCTC  >  NZ_CP034943/2680354‑2680629
                                                                                                                                         |                                                                                                                                          
cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCTGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTTCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGTTTGAGTATATTATTTTTCTTCTTGAAGGATTg                                                                                                                                          <  2:2958059/140‑1 (MQ=14)
cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                                                                                                          <  1:2591490/140‑1 (MQ=255)
cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                                                                                                          <  1:3552009/140‑1 (MQ=255)
cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                                                                                                          <  1:3649445/140‑1 (MQ=255)
cTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATg                                                                                                                                          <  1:4072778/140‑1 (MQ=255)
cTCCGGTTGACCCGCATCTTTCTCGTCCTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCTCTTCTTCTGCTTCTTGCCGGCGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGATTCTTCTGCTTCTTGACGGAATg                                                                                                                                          <  2:3886871/140‑1 (MQ=17)
    ggTTGAGCCGATTCTTTCTCTTCTTTACGGAGTGCGTGCTCAACGTGCTCCGTTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCAGGTTGAGCCGCTTCTTATGCTTCTGGACGGAATGCAgg                                                                                                                                      >  2:909304/1‑140 (MQ=17)
      ttGAGCCGCTTCTTTCGCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCt                                                                                                                                    <  2:1526741/140‑1 (MQ=255)
         aGCCGCTTCTTTGTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTGTGCTTCTTGACGGAGTGCCGGCGCAGCGTGCTGCGGTTGAGCCGCTGCGTGTGGTTCTTGCAGGTGTGCAGGCTCAg                                                                                                                                 >  2:591304/1‑140 (MQ=14)
         aGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAg                                                                                                                                 <  1:1271858/140‑1 (MQ=255)
           gcGCTTCTTTCTCTTCTTGACGGAGCGCCGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                                                                                                               <  1:1722490/139‑1 (MQ=255)
           ccGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                                                                                                               <  1:2876478/140‑1 (MQ=255)
           ccGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                                                                                                               >  1:2651297/1‑140 (MQ=255)
           ccGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCGTCTTCTGCTTGTTGACGGAATGCAGGCTCAGCg                                                                                                                               >  1:3891343/1‑140 (MQ=255)
           ccGCTTCTTGCTCTTCATGACGGAGTGCGGGCTCACCGTGCTCCGGTTGAGCCGCTTCTTCCGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCg                                                                                                                               <  1:1221733/140‑1 (MQ=255)
              gttctttctCTTCGTGAGGCAGTGCTGGCTCACCTTGCTCCGGGTGAGCCGCTTCTTCTGCTTCTTGACGGAGGGCGGGCTCTGCGTGCTCCGGTTGAGCCGCTTCTTCCGCTTATTGACGGAATGCAGGCTCAGCGTGc                                                                                                                            <  1:4042261/139‑1 (MQ=17)
              cttcttTCTCTTCTTGACGGAGTGCGGGCTCAACCTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCCGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCAGCTTCTTGACGGACTGCAGGCTCAGCGTGc                                                                                                                            >  2:545976/1‑140 (MQ=255)
               ttctttctCTTCTTGACGGAGTGCGGGCTCATCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCt                                                                                                                           <  1:1113360/140‑1 (MQ=255)
                   gtctcTTCCTGTCGGAGTGCGGGCCCCAGGTGGTCCGGGTGAGCCGCGTCGTCTGCGTCTGGTCGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCgg                                                                                                                       <  2:1235832/139‑1 (MQ=14)
                        ttcttGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAg                                                                                                                  >  1:1603498/1‑140 (MQ=255)
                        gtcttGCGGGAGTGCGGGCTCATCGTGCTCCGGTTGCGACGATTCTTCGTGTTCTTGACGCAGTGCGGTCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAg                                                                                                                  <  1:2024406/139‑1 (MQ=11)
                        gtcttGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCGGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAg                                                                                                                  <  1:3241589/139‑1 (MQ=255)
                           ttGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAgctg                                                                                                               >  2:1626321/1‑140 (MQ=255)
                                  aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt                                                                                                        >  1:549686/1‑140 (MQ=255)
                                  aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt                                                                                                        >  2:892419/1‑140 (MQ=255)
                                  aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttcttt                                                                                                        >  1:706738/1‑140 (MQ=255)
                                  aGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCATGACGGAGTGCCGGCTCAGCGTGCACCCGTTGAGCCGCTTCTTCTGCTTCTTGAGGGACTGCAACATCAGCGTCCTCCCGTTGAgctgctacgac                                                                                                        >  2:4087529/1‑135 (MQ=14)
                                    tGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttctttct                                                                                                      >  1:2445323/1‑140 (MQ=255)
                                    tGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCttctttct                                                                                                      >  1:840435/1‑140 (MQ=255)
                                    tGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCGTCGTGACGGAGTGCCGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTATGCTTCTTGACGGAATGCAGGCTCAGCGTGCGCCGGGTGCTGTGCttctgtct                                                                                                      >  1:2537366/1‑140 (MQ=14)
                                            cAACGTGCTCCGGTTGAGCCGCGTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGa                                                                                              >  1:4183435/1‑140 (MQ=255)
                                             aaCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGAc                                                                                             <  2:2668202/140‑1 (MQ=255)
                                              aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                            >  1:3943015/1‑140 (MQ=255)
                                              aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                            >  1:3063676/1‑140 (MQ=255)
                                              aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                            <  1:2903534/140‑1 (MQ=255)
                                              aCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTATGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                                                                            >  1:467691/1‑140 (MQ=255)
                                                   cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTGTGCTTCTTGACGGCATGCAGGCTCAGCGTGCTCCGGTGGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                                                                       >  1:3333603/1‑140 (MQ=255)
                                                   cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTTACGGAATg                                                                                       >  1:2553711/1‑140 (MQ=255)
                                                   cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                                                                       >  1:957464/1‑140 (MQ=255)
                                                   cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                                                                       >  2:4143050/1‑140 (MQ=255)
                                                       ggTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAgg                                                                                   >  2:627579/1‑140 (MQ=255)
                                                        gTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGc                                                                                  >  1:1379568/1‑140 (MQ=255)
                                                            aGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAg                                                                              <  2:3915589/140‑1 (MQ=255)
                                                            aGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAg                                                                              <  2:787404/140‑1 (MQ=255)
                                                              ccGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCg                                                                            >  1:2222277/1‑140 (MQ=255)
                                                              ccGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCAGCTTATTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGACTCAGCg                                                                            <  2:418342/140‑1 (MQ=255)
                                                                       ctGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGt                                                                   <  1:4143050/140‑1 (MQ=255)
                                                                           ttcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCGGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAg                                                               <  2:399063/140‑1 (MQ=255)
                                                                            tcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgc                                                              >  2:3837208/1‑140 (MQ=255)
                                                                            tcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgc                                                              >  2:3134342/1‑140 (MQ=255)
                                                                            tcttGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCAGGTTGAgc                                                              >  1:1854170/1‑140 (MQ=255)
                                                                            tcttGACGGAGTGAGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTATGCTTCTTGACGGAATGCATGCTCAGCGTGCTCCGGTTGAGCTGCTTGTTTCTCTTCTTTACGGAATGCAGGCTCAGCGTCTTCCGGTTGAga                                                              >  1:3253147/1‑139 (MQ=17)
                                                                              ttGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgctg                                                            >  2:1433433/1‑140 (MQ=255)
                                                                                gACGGAGCGCGGGCTCAGCGGGCTACGGGTGAGCCGCTTCTTCTGCTAATTGACTGACTGCAGGCTCAGAGTGCTCCTGTTGAGCTGCTAATTTCTCTTCGTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAgctgct                                                          <  1:1801960/140‑1 (MQ=17)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGATTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttctttct                                                   <  2:1194302/140‑1 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGc                                                                                                                                     >  1:3910472/1‑58 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCtcttct                                                                                                 <  1:2759105/94‑1 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCtcttct                                                                                                 >  2:2759105/1‑94 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCtcttct                                                                                                 <  1:3856198/94‑1 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttctttct                                                   <  2:2096778/140‑1 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttctttct                                                   <  2:2207708/140‑1 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttctttct                                                   >  1:2709293/1‑140 (MQ=255)
                                                                                       tGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCttctttct                                                   >  1:809141/1‑140 (MQ=255)
                                                                                               cAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGa                                           <  1:1314268/140‑1 (MQ=255)
                                                                                               cAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGa                                           >  2:644669/1‑140 (MQ=255)
                                                                                                aGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGAc                                          >  1:2385341/1‑140 (MQ=255)
                                                                                                aGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGAc                                          <  2:771944/140‑1 (MQ=255)
                                                                                                 gCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTGTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTGTTGACg                                         >  2:1819610/1‑140 (MQ=255)
                                                                                                 gCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACg                                         >  2:2396249/1‑140 (MQ=255)
                                                                                                  cttGCTCAGGTTGAGCCGCGTCTTTAGCTTCTTGACGTACTGCAGGCTCAGCGTGGTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACgg                                        <  2:2002269/138‑1 (MQ=14)
                                                                                                  cGTGCTCCGTTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACgg                                        <  2:2695254/140‑1 (MQ=255)
                                                                                                      cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTGTCTTCTTGACGGAATGCAGGCTCCGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGTATg                                    >  1:1592571/1‑140 (MQ=255)
                                                                                                      cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCt                                                                         <  1:10543/103‑1 (MQ=255)
                                                                                                      cTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATg                                    >  2:2255033/1‑140 (MQ=255)
                                                                                                      cTCCGGTTGAGCCGCTTCTTCTGCGTCTTGACGGAATGCAGGCTCAGCGTGCGCCGGTTGAGCGGCTTCGTTCTCTTCTTGACGGAATGCCGGCTCAGCGTCTTCCGGGTGAGCGGCTTCTTTCTCTTCTTGACGGAATg                                    >  1:1667888/1‑140 (MQ=14)
                                                                                                       tCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGACTGc                                   >  2:1688658/1‑140 (MQ=255)
                                                                                                           gTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGACTGCAGGc                               >  2:2452203/1‑140 (MQ=255)
                                                                                                           gTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGc                               >  2:2596385/1‑140 (MQ=255)
                                                                                                           gTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGc                               >  2:2291213/1‑140 (MQ=255)
                                                                                                            ttGCGCCGCTTCTTCTGCTTCCTGACGGACTGCCGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGTCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCt                              <  2:1463644/140‑1 (MQ=14)
                                                                                                            ttGAGCCGCTTCTTCTGCTTCTTGACGGACTGGCGGCTCAGCGTGCTCCGGTTGAGGTGCTTCTTGCGATTCTTGACGGAATGCAGGCTCAGCGTGTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCt                              <  2:3509918/140‑1 (MQ=11)
                                                                                                            ttGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCt                              >  2:523262/1‑140 (MQ=255)
                                                                                                            ttGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCt                              >  2:957380/1‑140 (MQ=255)
                                                                                                              gAGCCGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGAGGGAATGCAGGCTCa                            >  2:1938966/1‑140 (MQ=255)
                                                                                                               ctccGCTTCTTCTGCGACTTGACGGAATGCAGGCTCAGCGTGCTCCTGTTGTGGTTCTTCTTTCTCTTCTTGACGGACTGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAg                           <  2:2445323/138‑1 (MQ=17)
                                                                                                               aGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAg                           >  1:76434/1‑140 (MQ=255)
                                                                                                               aGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGGTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGTTTGAGCTGCTTCTTTCTCTTCTTGTCGGAATGCAGTCTCat                           >  2:3198157/1‑139 (MQ=255)
                                                                                                               aGCCGCTTCTTCGGCTTCTTGACGGCAGGCCGGCTCAGCGTGCTCCGGTTGAGCGGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAg                           <  2:2537366/140‑1 (MQ=14)
                                                                                                                 ccGCTTCTTCTGCTTCTTGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCg                         <  1:89909/140‑1 (MQ=255)
                                                                                                                    cgtcttctGCTTCATGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGc                      <  1:1483486/138‑1 (MQ=255)
                                                                                                                     ttcttgTGCTTCATGACTGATTGCAGGCTCAGCTTGCTCCGGTTGTGCTGCTTCTTTCTCTTCGTGACGGAATGCAGGCTCTGCGTGTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCt                     <  2:1079815/140‑1 (MQ=14)
                                                                                                                     ttcttcTGCTTCATGACGGACTGCCGGCTCAGCGTGCTCCGCTTGAGCTGCTTCTTTCTCTTCTGGACGGAATGCAGGCTCAGCGTCTTCCGCTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTggt                     <  2:1594389/140‑3 (MQ=14)
                                                                                                                             cttcttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGTATGCAGGCTCAGCGTGCTCCGGTGGa             >  2:3736122/1‑140 (MQ=255)
                                                                                                                             cttcttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGa             >  2:334846/1‑140 (MQ=255)
                                                                                                                             cttcttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTACTCTTCATGAGGGACTGCAAGCTCAGCGTCATCCGGTTGAGCTGCTTGTTTCTCTTCTTGAAGGACTGCACGCTCAGCGTGCTCCTGTTGa             >  2:3950478/1‑140 (MQ=17)
                                                                                                                              ttcttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCATTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAg            <  2:716822/140‑1 (MQ=255)
                                                                                                                                 ttGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCg         <  1:644669/140‑1 (MQ=255)
                                                                                                                                     cGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTGCTCTTCTTGACGGCATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCATGACGGACTGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTc     <  2:2826779/140‑1 (MQ=255)
                                                                                                                                      ggACTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTcc    <  1:2725576/140‑1 (MQ=255)
                                                                                                                                        aaTGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTCCTc  >  2:463910/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CTCCGGTTGAGCCGCTTCTTTCTCTTCTTGACGGAGTGCGGGCTCAACGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAGTGCGGGCTCAGCGTGCTCCGGTTGAGCCGCTTCTTCTGCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTCTTCCGGTTGAGCTGCTTCTTTCTCTTCTTGACGGAATGCAGGCTCAGCGTGCTCCGGTTGAGCCGCTTCCTC  >  NZ_CP034943/2680354‑2680629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: