Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,980,283 T→G 18.7% noncoding (518/2928 nt) EO946_RS15430 ← 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,980,2830TG18.7% 2.1 / 3.8 50noncoding (518/2928 nt)EO946_RS1543023S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (2/37);  new base G (2/9);  total (4/46)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTAC  >  NZ_CP034943/2980144‑2980419
                                                                                                                                           |                                                                                                                                        
cttcTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACCAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCtt                                                                                                                                          <  2:866169/137‑1 (MQ=1)
       acattGCACGGGATCGTAACTGGCCGGTTCATTGTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTAGTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCa                                                                                                                                   <  1:2901440/137‑1 (MQ=0)
       acattGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCAATACACTTCCGGGGTGCTTTTCACCTGTCCCTCa                                                                                                                                   <  2:838116/137‑1 (MQ=1)
           tgcaaaggATCGTAACTCGCCGGTTCATTCTCCAAAAGGCACGCCATCACCCGGGAACGGGCTCTGACTAGTTGTAGGCAGACGGTGTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTGTCCCTCTCGGt                                                                                                                               >  2:2073875/7‑140 (MQ=1)
             catgggATCGTAACTCGCCGGTGCATTCTACATAAGGCACGCCATCACCCGTTAACGGGCTATGAATACTTTTAGTCACACGGTTTCAGGATCTGTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAc                                                                                                                             <  2:3588353/137‑1 (MQ=1)
              acaggATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACt                                                                                                                            <  1:1789393/137‑1 (MQ=1)
              acaggATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACt                                                                                                                            <  1:364228/137‑1 (MQ=1)
                ggtatagTAACTCGCGGGTTCATTCTACAAAAGGCACGCCATCACGCGTTAAGGGGCTCTGACTACGTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCAAGGTACTgg                                                                                                                          <  2:3926588/134‑1 (MQ=1)
                  gagcGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGtt                                                                                                                        <  1:4230576/137‑1 (MQ=1)
                              cctgTTCATTCTACAAAAGGCACGCCATCACCAGTTAACGGGCTCTGAATACTTTTAGGCACACGGTTTCAGGATCTATTTCAATCACATTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCa                                                                                                            <  2:3072988/137‑1 (MQ=1)
                                gggtCATTCTACAAAAGTCAAGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTACACTTCCGGGGTGCTTTTCACCTTTCCCTCAAGGTACTGTTTCAATATCGGTCACt                                                                                                          <  2:3199828/137‑1 (MQ=1)
                                gggtCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACt                                                                                                          <  2:1750334/137‑1 (MQ=1)
                                gggtCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTACGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACt                                                                                                          <  2:1207414/137‑1 (MQ=1)
                                   tccttCTACAACAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAgg                                                                                                       <  1:3259300/137‑1 (MQ=1)
                                    cagtcgacaacaCGCACGCCCTCCCCCGTTACCGGGCTCTCACGACGTGGAGGCACCCGGTGTCAGGATCTCTTGCACTCCCCTGCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAggg                                                                                                      <  2:2575866/134‑1 (MQ=1)
                                    cagtCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAggg                                                                                                      <  2:3484585/137‑1 (MQ=1)
                                    cagtCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAggg                                                                                                      <  2:1212375/137‑1 (MQ=1)
                                           cacaacgCACCCCCTCACCCGCTAACGGGCTCTGACTACGTGTAGGCACCCGGTTGCCGGATCGCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCCCGGTACTGGTTCACTATCGGTCACTAGGGAGTAttt                                                                                               <  2:2417341/134‑1 (MQ=1)
                                           cacaaGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTAttt                                                                                               <  2:3188411/137‑1 (MQ=1)
                                           cacaaGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGGAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTGTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTAttt                                                                                               <  2:1233400/137‑1 (MQ=0)
                                            aacaGGCTCGCCATCACCCGTGAACGGGCTCTGACTACTGGGAGGCACACGGTTTCAGGATCTCTTTGACTCCCCTGCCGGGGTGCTTTTCAGCTTTCCCTCACGGTACTTGTTCACTATCGGTCACTAGGGAGTATTTa                                                                                              <  1:513776/137‑1 (MQ=1)
                                            aacaGGCACGCCATCACCCGTTAACCGGCTCTGACTACTTGGAGCCACACGGAGTCAGCATCTCTTACACACCCCTTCCGGGGTGCTGTTCACCTTACCCTCACGGTACTGGTTCACGATCGGTCACTAGGGAGTCTTTa                                                                                              >  2:397182/4‑140 (MQ=1)
                                                                ttcaCGGGCTCTGACTACTTGTCGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTGTCCCTCCCGGTACGGGTTCACTATCGGTCCGTAGGGAGTCTTTAGCCTTGGGAGATGGtcctcc                                                                          >  2:391639/4‑140 (MQ=1)
                                                                                acgtGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAt                                                          <  1:1601543/137‑1 (MQ=1)
                                                                                acgtGTAGGCACACGGTTTCAGGATCTCTGTCACGCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACGGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAt                                                          <  1:4085950/137‑1 (MQ=0)
                                                                                   tggaGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTGTTCACCTGTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTc                                                       <  2:2938935/137‑1 (MQ=0)
                                                                                          actcGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGtt                                                <  2:551614/137‑1 (MQ=1)
                                                                                          actcGGTTTCACGATCTCTTTCACTCCCCTTCCGGGGTGCTTGTCACCTTTCCCGCCCGGTACTGGTTCCCTATCCGTCACTAGGGAGTATTTAGCCATGGGAGATGGGCCTCCCGGATTCCGACGGAATTTCACGTGtt                                                <  2:536094/137‑1 (MQ=1)
                                                                                          actcGGTGTGAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGtt                                                <  2:1127210/137‑1 (MQ=0)
                                                                                          acccGGTTTGAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCCCGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGAGGGTCCTCCCGGATTCCGACGGAATTTCACGTGtt                                                <  1:3804291/137‑1 (MQ=0)
                                                                                              gggttCAGGATCTCTTTCACGCCCCTTCCGGGGTGCTTTTCACCTGTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTccgc                                            <  1:1672353/137‑1 (MQ=0)
                                                                                               gtgtCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTccgcc                                           <  1:617915/137‑1 (MQ=1)
                                                                                                     ggctctcTTTCACTCCCCTTCCGGGGTGCTTTGCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATGTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTc                                     <  2:1863029/137‑1 (MQ=0)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCCCGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:4261044/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:2570867/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:1566903/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:1158051/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:3178431/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTGTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  1:3313579/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGGGTGCTTATCCCCGTGCCCTCCCGCTACTGGTTCACTATCGGTCACTAGGGAGTATTTACCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:1995120/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCGGCGTGCGTGTCACCGTTCCCTCACGGTTCTGGTTCACTATCGGTCACTAGGGAGTATTTACCCTTGGGAGATGGTCCTCCCGGAGTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:3730566/137‑1 (MQ=1)
                                                                                                          tcgttCACTCCCCTTCCCGGGTGCTTTTCACCTTGCCCTCACGGTACGGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  1:4106967/137‑1 (MQ=0)
                                                                                                          tcgttCACTCCCCGTCCGGGGGGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:2358117/137‑1 (MQ=0)
                                                                                                          tcgttCACGCCCCTTCCGGGGTGCTTTTCACCGTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTGGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:4121260/137‑1 (MQ=0)
                                                                                                          tcggtcccTCCCCTTCCGGGGTGCGTTTCACCGGTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:3093445/133‑1 (MQ=1)
                                                                                                          tccttCACTCCCCGACCGGGGTGCTTTTCACCTGTCCCTCACGGTACTGGGTCACTATCGGTCACTAGGGAGTATTTAGCCGTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:3123608/137‑1 (MQ=1)
                                                                                                          tcattCACTCCCCTTCCGGGGTGCTTTACACCTTTCCCTCACGCTACTGGTAAACTATCGGTCACTAGGGAGTATATAGCCTTTGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                >  2:850321/4‑140 (MQ=1)
                                                                                                          gctgtCACTCCCCGTCCGGGGTGCTTTTCACCTGTCCCTCCCGGTACTGGTTCACGATCGGGCACTAGGGAGGATGTAGCCGAGGGAGAGGGTCCTCGCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGAt                                <  2:3489524/136‑1 (MQ=1)
                                                                                                                                  ttgtCACCTTTCCCGCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGtttt        <  2:2917713/137‑1 (MQ=1)
                                                                                                                                  ttgtCACCTGTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGAGGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGtttt        <  2:3404928/137‑1 (MQ=0)
                                                                                                                                  ttatCACCTGTCCCTCACGGTACTGGTTCACTAACGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGAGTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGtttt        <  1:3131930/137‑1 (MQ=0)
                                                                                                                                        ccgttCCCTCCCGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTAc  <  2:3296052/137‑1 (MQ=1)
                                                                                                                                        ccgttCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTAc  <  1:2127157/137‑1 (MQ=1)
                                                                                                                                           |                                                                                                                                        
CTGCTTAACCTTGCACGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTACTCAGGATCCACTCAGGAGAGAACGAAGTTTTGACTAC  >  NZ_CP034943/2980144‑2980419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑