Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,184,726 A→C 5.3% intergenic (+22/+15) mdtP → / ← EO946_RS16485 multidrug efflux MFS transporter MdtP/tautomerase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,184,7260AC 5.3% 660.2 / 3.1 274intergenic (+22/+15)mdtP/EO946_RS16485multidrug efflux MFS transporter MdtP/tautomerase family protein
Reads supporting (aligned to +/- strand):  ref base A (133/116);  new base C (20/5);  total (153/121)
Fisher's exact test for biased strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

TATCGCGGTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGC  >  NZ_CP034943/3184587‑3184865
                                                                                                                                           |                                                                                                                                           
tATCGCGGTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCaa                                                                                                                                             >  1:1484396/1‑140 (MQ=255)
tATCGCGGTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCaa                                                                                                                                             >  1:1457906/1‑140 (MQ=255)
    tgggTCGGAGCCGTATTTACTTTGTTATTAAAACCTATAAAACTATGAAACAAGAAAACGGGAGAACAAGTAAAAGAGTATTGGTCACAAGCCTTTGAATCACCTTCACACTAATAGACCAACCAGCGGGTATCACTcag                                                                                                                                         <  2:2019274/138‑3 (MQ=255)
    gcgGTCGGAGGCGTATTTACTTTTTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCcg                                                                                                                                         <  1:3999179/140‑1 (MQ=255)
    gcgGTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCcg                                                                                                                                         <  1:3732302/140‑1 (MQ=255)
     cgGTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCcgc                                                                                                                                        >  2:819288/1‑140 (MQ=255)
      ggTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAACCCAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACACGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCcgcg                                                                                                                                       <  2:2630553/140‑1 (MQ=255)
       gTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTCTCACTCCGCgg                                                                                                                                      >  2:1699281/1‑140 (MQ=255)
       gTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCgg                                                                                                                                      >  2:4171111/1‑140 (MQ=255)
       gTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCgg                                                                                                                                      >  2:2097311/1‑140 (MQ=255)
       gTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGATGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAAACCGCgg                                                                                                                                      >  1:871121/1‑140 (MQ=255)
         cGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAACCAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCgggg                                                                                                                                    <  1:3634912/140‑1 (MQ=255)
              ccGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTtta                                                                                                                               <  1:3590163/140‑1 (MQ=255)
                 tATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTttatta                                                                                                                            <  2:230461/140‑1 (MQ=255)
                 tATTTACTTTGGTCTTAAAACCTATCAAACTATCATACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACACGCCGTTGAATCTCCTTCCCAGTAATAGCCCACCCCGCGGGTCTCCCTCCGGGGGGTGgtatta                                                                                                                            >  2:1582636/1‑140 (MQ=255)
                  aTTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTGTtattat                                                                                                                           >  1:609502/1‑140 (MQ=255)
                  aTTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCGTCCCATTAAGAGACCACCCCGCGGGTATCAATCCGCGGGGTTTtattat                                                                                                                           >  1:1045582/1‑140 (MQ=255)
                     tACTTTGTTCTTAACAACTATCAACCTATCAAACTAGAAAACGGGAGAACATGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCTCGGGTATCAATACGCGGGGTTTTATTATTCa                                                                                                                        <  1:4197373/140‑1 (MQ=255)
                     tACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCa                                                                                                                        <  2:2679150/140‑1 (MQ=255)
                      aCTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCaa                                                                                                                       >  2:138641/1‑140 (MQ=255)
                      aCTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCaa                                                                                                                       <  1:1236513/140‑1 (MQ=255)
                         ttGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATAACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAatca                                                                                                                    >  2:3444452/1‑140 (MQ=255)
                          tGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCCATCCGCGGGGTTTTATTATTCAatcat                                                                                                                   >  1:424566/1‑140 (MQ=255)
                          tGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAatcat                                                                                                                   <  2:1282270/140‑1 (MQ=255)
                          tGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAatcat                                                                                                                   >  2:1140690/1‑140 (MQ=255)
                          tGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACATTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAatcat                                                                                                                   >  2:4000497/1‑140 (MQ=255)
                           gTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAatcatc                                                                                                                  >  2:3870389/1‑140 (MQ=255)
                           gTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAatcatc                                                                                                                  >  1:2440368/1‑140 (MQ=255)
                            ttCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCt                                                                                                                 >  1:3129619/1‑140 (MQ=255)
                             tCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTg                                                                                                                <  2:1973259/140‑1 (MQ=255)
                               tgaaaaCCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCCCCCCGCGGGTATCAAGCCGCGGGGTTTTATTATTCAATCATCTggg                                                                                                              <  2:4067115/138‑1 (MQ=255)
                                         tcaaactatcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCAtt                                                                                                    >  1:938419/1‑140 (MQ=255)
                                                atcaaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGaaa                                                                                             <  1:3317816/140‑1 (MQ=255)
                                                   aaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAc                                                                                          <  1:681550/140‑1 (MQ=255)
                                                   aaacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAc                                                                                          >  1:1783744/1‑140 (MQ=255)
                                                    aacAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCGTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGATTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAcc                                                                                         <  1:2813217/140‑1 (MQ=255)
                                                         gAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATcc                                                                                    >  2:3321318/1‑140 (MQ=255)
                                                          aaaaCGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCt                                                                                   <  1:3691275/140‑1 (MQ=255)
                                                              cGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATc                                                                               <  2:804904/140‑1 (MQ=255)
                                                                ggAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTACCGCCCGACCAATCCTCATCCt                                                                             <  2:901214/140‑1 (MQ=255)
                                                                    aaCAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCCATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGAtt                                                                         >  2:548947/1‑140 (MQ=255)
                                                                    aaCAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCCTCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGAtt                                                                         >  2:3424057/1‑140 (MQ=255)
                                                                          tAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCAATTAAATTTACTATATAACAATTCTCATACTGAtttagtgg                                                                   <  2:3135578/140‑6 (MQ=255)
                                                                              atagaAACAGGCACAAGGCGTTGAATCAACTTCCCATTAATAGACCACCACTCGGGTATCAATCCTCGGGTTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGc                                                               <  2:2511412/138‑1 (MQ=255)
                                                                              agagAAAGCGGCACAATCCGTTGAATCACCTTCCCCTTAATACACCACCCCGCGGGTATCACTCCGCGCGGTTATATTATTCACTCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGc                                                               <  1:2090384/140‑1 (MQ=255)
                                                                              agagAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGc                                                               <  2:1738574/140‑1 (MQ=255)
                                                                              agagAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGc                                                               <  2:393809/140‑1 (MQ=255)
                                                                              agagAAAGCGGCACAAGCCGTTGAATCACCGTCCCATTAAGAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGc                                                               <  1:1699281/140‑1 (MQ=255)
                                                                                   aaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGAt                                                          >  1:3032841/1‑140 (MQ=255)
                                                                                   aaGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGAt                                                          >  1:1612343/1‑140 (MQ=255)
                                                                                    aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGttt                                                                                                                                 <  1:3192659/68‑1 (MQ=255)
                                                                                    aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGttt                                                                                                                                 >  2:859353/1‑68 (MQ=255)
                                                                                    aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGttt                                                                                                                                 >  2:3192659/1‑68 (MQ=255)
                                                                                    aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATa                                                         <  1:3166518/140‑1 (MQ=255)
                                                                                    aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATa                                                         >  1:1554534/1‑140 (MQ=255)
                                                                                    aGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCAGCCTGATTGCCGGCCAGCATGaga                                                         >  1:2170539/1‑138 (MQ=255)
                                                                                        gCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATGTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAaca                                                     <  2:3125463/140‑1 (MQ=255)
                                                                                         cacaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGa                                                                                               <  1:3867277/97‑1 (MQ=255)
                                                                                         cacaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAacac                                                    <  1:1141522/140‑1 (MQ=255)
                                                                                         cacaAGCCGTTGAATCAACTTCCCATTAATAGACCACCCCGAGGTTATCAATCAGAGGGTTATTATTATTCAATCATCTGAGCCCTTACATTTCCGAAAGACCAATCCTCATCCTGATTTCCTGCCATCATGATAAacac                                                    <  2:177916/140‑1 (MQ=255)
                                                                                          acaAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAACGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAAcaca                                                   <  1:2951806/140‑1 (MQ=255)
                                                                                                   tGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCAGCCTGATTTCCGGCCAGCATGATAAACACATCttctttt                                          >  1:2876807/1‑140 (MQ=255)
                                                                                                      aTCACCTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttcttttctt                                       >  1:1447682/1‑140 (MQ=255)
                                                                                                      aTCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCttcttttctt                                       <  1:138641/140‑1 (MQ=255)
                                                                                                      aTCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCAGATTTCCGGCCAGCATGATAAACACATCttcttttctt                                       >  2:3843260/1‑140 (MQ=255)
                                                                                                          ccTTCCCATTAATAGACCACCCCGCGGGTCTCACTCCGCGGGGTTTTATTATTCAATCATCTCGGCCCTTCCATTTCCGAATGACCAATCCTCATACTGATTTCCGGCCAGCATCATAAGAACATCATCTTTTCTTACGc                                   >  2:2474032/1‑140 (MQ=255)
                                                                                                          ccTTCCCATTAATAGACCACCCCGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTGTCTTACGc                                   >  1:4146571/1‑140 (MQ=255)
                                                                                                          ccTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGc                                   <  1:2317802/140‑1 (MQ=255)
                                                                                                          ccTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTATTAAGc                                   >  1:3963536/1‑140 (MQ=255)
                                                                                                           cTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGcc                                  <  2:3032841/140‑1 (MQ=255)
                                                                                                                cATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATGATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCt                             <  2:2605130/140‑1 (MQ=255)
                                                                                                                cATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCt                             <  1:2217855/140‑1 (MQ=255)
                                                                                                                     aTAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCg                                                                                                <  1:2580070/68‑1 (MQ=255)
                                                                                                                     aTAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGAc                                                                                          <  2:4021473/74‑1 (MQ=255)
                                                                                                                     aTAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaa                        >  1:1846340/1‑140 (MQ=255)
                                                                                                                         accaccCCGCGGGTCTCCAGCCGCGGGGTGTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAAGCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCGTCTTTTCTTACGCCACGCTCGgactgaa                    >  1:3918590/1‑140 (MQ=255)
                                                                                                                         accaccCCGCGGGTATCCATCCGCGGGGTTTTATTATTCAAGCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatgaa                    >  2:1500667/1‑140 (MQ=255)
                                                                                                                         accaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGgaatgaa                    >  1:896091/1‑140 (MQ=255)
                                                                                                                         accaccCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGACCTTCCATTTCAGAAAGACCAATCCTCATCCTGATTTCCGGCGAGCATGATAAACTCATCTTCTTTTCTTACGCCAAGCTCGgaatgta                    >  2:2976355/1‑138 (MQ=255)
                                                                                                                         accaccCCGCGGGTATCAATCAGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTAAGCCAAGCTCGgaatgaa                    >  1:2378062/1‑140 (MQ=255)
                                                                                                                         accacaCCGCGAGTATCACTCCCCGGGGTATTATAATTCAAAAATCAGGGCCCTTCCATTTCAGAAAGACAAATACTCATCCTGATATCCGGCCAGCTTGATAAAAACATATTCTTTTCTTACGCCAAGCTCGgaatgaa                    >  2:96916/1‑140 (MQ=255)
                                                                                                                             ccccGCGGGTATCCATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCCAGCCTCATCCTGATTTCCGGCCAGCATGATAAACACAGCTTCTGTTCTTCCGCCAAGGTCGGAATGAATCCg                >  2:2848676/1‑140 (MQ=255)
                                                                                                                             ccccGCGGGTATCACTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                >  1:1669821/1‑140 (MQ=255)
                                                                                                                             ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGTTAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                >  2:2727149/1‑140 (MQ=255)
                                                                                                                             ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                >  2:3603318/1‑140 (MQ=255)
                                                                                                                             ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                >  2:717615/1‑140 (MQ=255)
                                                                                                                             ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCg                >  1:2718062/1‑140 (MQ=255)
                                                                                                                             ccccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCCATCCTCATCCTGATTTCCGGCCAGCCTGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGACTCCg                >  1:487336/1‑140 (MQ=255)
                                                                                                                              cccGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCgg               >  1:3487704/1‑140 (MQ=255)
                                                                                                                              cccGCGGGTAACAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCATTCCCTTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTGCTTTGCGTCCGCCACTCTCGGAATGAATCCgg               >  2:777575/1‑140 (MQ=255)
                                                                                                                                cgcgGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCCGCATGATAAACACAACTTCGTTTCTTACGCCAAGCTCGGAATGAATCCGGCt             >  1:4091984/1‑140 (MQ=255)
                                                                                                                                     gTATCCCTCCGCGGGGCTTTATTATTCAAGCATCTGGGCCCGTCCAGTTCCGAAACACCAATCCTCAGCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCACGCTCGGAATGAATCCGGCTTGCGa        <  1:2258438/140‑1 (MQ=255)
                                                                                                                                     gTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGa        <  1:2126643/140‑1 (MQ=255)
                                                                                                                                       aTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt      <  1:565154/140‑1 (MQ=255)
                                                                                                                                       aTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt      <  2:129967/140‑1 (MQ=255)
                                                                                                                                       aTCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGAtt      <  1:1954365/140‑1 (MQ=255)
                                                                                                                                          taTCCGCGGGGTTTTTTTATTCAATCATCTGGGCCCTTCCATGTCCGAAAGCCCAATCCTCAGCCTGATTTCCGGCCAGCATGATACACACATCTTCTTTTCTTACGCCAAGGTCGGAATGAATCCGGCTTGCGATTGAg   <  2:3716952/139‑1 (MQ=255)
                                                                                                                                          aaTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAg   <  1:84501/140‑1 (MQ=255)
                                                                                                                                          aaTCCGCGGGGTTTTATTATTCAATCATCTGGGCCCGTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAg   <  1:1007748/140‑1 (MQ=255)
                                                                                                                                          aaTCCGCGGGGTTATATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAg   <  2:485514/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TATCGCGGTCGGAGCCGTATTTACTTTGTTCTTAAAACCTATCAAACTATCAAACAAGAAAACGGGAGAACAAGTAAAAGAGAAAGCGGCACAAGCCGTTGAATCACCTTCCCATTAATAGACCACCCCGCGGGTATCAATCCGCGGGGTTTTATTATTCAATCATCTGGGCCCTTCCATTTCCGAAAGACCAATCCTCATCCTGATTTCCGGCCAGCATGATAAACACATCTTCTTTTCTTACGCCAAGCTCGGAATGAATCCGGCTTGCGATTGAGC  >  NZ_CP034943/3184587‑3184865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: