BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,190	G→T	7.6%	F114L (TTC→TTA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,190	0	G	T	7.6%	676.1 / 6.6	289	F114L (TTC→TTA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (129/126); new base T (32/2); total (161/128)
Fisher's exact test for biased strand distribution p-value = 4.48e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

GCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAT  >  NZ_CP034943/240050‑240325

    cTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAg                                                                                                                                      >  2:2062359/1‑140 (MQ=255)

      gtggtgGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGtt                                                                                                                                    >  2:3748384/1‑140 (MQ=255)

         gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGttttt                                                                                                                                 >  1:2512086/1‑139 (MQ=255)

         gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTg                                                                                                                                 >  1:3468838/1‑140 (MQ=255)

           ggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTtgg                                                                                                                               >  1:1808235/1‑140 (MQ=255)

           ggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTggg                                                                                                                               >  1:978074/1‑140 (MQ=255)

              gaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGgaga                                                                                                                            >  2:1270010/1‑140 (MQ=255)

                 ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAttt                                                                                                                         >  2:2090470/1‑140 (MQ=255)

                    gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                      <  1:1776752/140‑1 (MQ=255)

                    gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                      <  2:2882178/140‑1 (MQ=255)

                    gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTAGTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                      <  2:348491/140‑1 (MQ=255)

                     tAAGAGAGAGAGAAGGAGAATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAt                                                                                                                     <  2:1441158/140‑1 (MQ=255)

                         agagagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATAAGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATGGCt                                                                                                                 >  2:872236/1‑140 (MQ=255)

                           agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGTGAGATTTGAATGGCttt                                                                                                               >  2:2926862/1‑140 (MQ=255)

                           agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCttt                                                                                                               >  2:3044715/1‑140 (MQ=255)

                            gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCtttt                                                                                                              >  2:2956266/1‑140 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTTGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATAAGAAGTCTATCATTCCGACTATAAGTGCGTTTTCGGAATTTTTTTTGAATTTTTTGGAGATTTTAAATTCTTTTc                                                                                                             >  2:627367/1‑140 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACTGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTc                                                                                                             >  2:2730181/1‑140 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGTTTTGAATGGCTTTTc                                                                                                             >  1:3009855/1‑140 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGTAGTTTTGGGCGATTTGAATgtgttgtg                                                                                                             >  1:3514307/1‑133 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTc                                                                                                             >  2:162627/1‑140 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATGGCtttta                                                                                                             >  2:1806441/1‑139 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATTGCtttta                                                                                                             >  2:1228346/1‑139 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGGttttg                                                                                                             >  1:1025251/1‑139 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                             >  2:1134630/1‑140 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGAGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTTAATTGCgttta                                                                                                             >  2:3574237/1‑139 (MQ=255)

                             agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGAGGCTGTTTTTGTATTTTTGTTCTAATACTAAGTCGATAATTACGACTATACGGGCGTTTTGGGATTTTTTTTTGTAGTTTTGTGATATTTGAATGTCTGTTc                                                                                                             >  1:2003967/1‑140 (MQ=255)

                               agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCg                                                                                                           >  2:2651721/1‑140 (MQ=255)

                                    gAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGttttt                                                                                                      >  2:1894410/1‑140 (MQ=255)

                                       cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTaaa                                                                                                   >  2:2265700/1‑140 (MQ=255)

                                         cctctGATGTTGGAATTATTCAGACAGGCTGTTTTGGAATGTGTGTTCTAAGCCGCAGTCGAACAGGCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                 <  1:3432576/139‑1 (MQ=255)

                                          ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                <  1:3044715/140‑1 (MQ=255)

                                               aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAgg                                                                                           >  2:2121587/1‑140 (MQ=255)

                                               aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                           >  2:1548528/1‑140 (MQ=255)

                                               aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGATTTTAAAACAAGAgg                                                                                           <  1:1348066/140‑1 (MQ=255)

                                               aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTACGTTTTTAAAACAAGAgg                                                                                           >  1:2072166/1‑140 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGATATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCCAGc                                                                                      >  2:3248179/1‑140 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGTTTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                      >  2:944374/1‑140 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                      >  2:1831583/1‑140 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTGCGTTTTTTAAAAAATATTCAAGc                                                                                      >  2:2431985/1‑140 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGc                                                                                      >  2:3875369/1‑140 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                      >  2:1535198/1‑140 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTGGGGGGTTTGAATGGGTTTTCCGTTTTTGAAAAAAGAGGccatc                                                                                      >  1:3889825/1‑136 (MQ=255)

                                                    ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTGGAAGTTTTGGGAGAATTTAATTGCTTTTACGTTTTTAAAAAAAGaagcaagc                                                                                      >  2:308830/1‑140 (MQ=255)

                                                        ttattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTGTTCCGTTTTTAAAACAAGAGGCAATCCAGa                                                                                  >  1:825460/1‑140 (MQ=255)

                                                          agcacgagaTGCTGTTTGTGTTGTTTTTTTCTACTACGAATTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGATGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                <  1:2836100/135‑1 (MQ=255)

                                                           ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAACAGGCAAGCCAGaaaa                                                                               >  1:3131227/1‑140 (MQ=255)

                                                           ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATaa                                                                               <  1:118885/140‑1 (MQ=255)

                                                                     cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGTAGTTTTTGGATATTTGTATTGCGTTTCAGGTTTTAAAACAAGATGCAAGCCAGATAAAACCCacaaa                                                                     >  1:2651822/1‑139 (MQ=255)

                                                                     cTGTTTTTGTATTTTGGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTTGGATTTTTGTTTGAAGGTTTTGGCGGTTTGTATGGGGTTTGCGTTTTGAATACACGAGGCAAGCCAGCTAAAACCGaccac                                                                     >  1:1645756/1‑140 (MQ=255)

                                                                          tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTGAACTTTTTTGAGATTTTAATTGCTTTTCAGTTTTTAAAAAAAGAGGCAACACAGATAAAACCGAcaacaaaaa                                                                >  1:729737/1‑140 (MQ=255)

                                                                                  tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                        >  2:784198/1‑140 (MQ=255)

                                                                                    tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                      <  2:1012421/140‑1 (MQ=255)

                                                                                     gTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTa                                                     <  2:1337464/140‑1 (MQ=255)

                                                                                     gTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTa                                                     <  2:3787278/140‑1 (MQ=255)

                                                                                      ttCTCAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                    <  1:2361258/140‑1 (MQ=255)

                                                                                      ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTGTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                    <  2:3325349/140‑1 (MQ=255)

                                                                                      ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                    <  2:1485126/140‑1 (MQ=255)

                                                                                      ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                    <  2:2604578/140‑1 (MQ=255)

                                                                                       tatAATACGAAGTCGATCATTCCGAATATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGATATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGc                                                   <  2:1698131/138‑1 (MQ=255)

                                                                                           aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                               <  1:3967927/140‑1 (MQ=255)

                                                                                           aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                               <  2:3372444/140‑1 (MQ=255)

                                                                                            tACGAAGTCGTTCAGTCCGACTATACGGGCTTTTTCGTATTTTTGATTGAAGTTTTGGGTGATTTGAATGGCTTTTCAGTTTTTAAAACAAGAGGAAAGCCAGATAAACCCTACAACAACAAAAAATAATCTAGCAAGTa                                              <  2:1247377/140‑1 (MQ=255)

                                                                                            tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                              <  1:2153801/140‑1 (MQ=255)

                                                                                            tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                              <  1:2697691/140‑1 (MQ=255)

                                                                                              cGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaaa                                            <  1:3810112/140‑1 (MQ=255)

                                                                                                aaGTCGATAATTCCGACTATACGGGCGTTTTAGGAATTTTGTTTGAAGTTTTGGGAGATTTGTATGGCTTTTCCGTTTTTAATAAAAGTTGCCAGCCAGATAAAGCCGAAAACAACCAAAAATACTCTAGCACGTACACt                                          >  2:2511218/1‑140 (MQ=255)

                                                                                                 attCGATCATTCCGACTATACGGGCGTTTTATGATTTTTGTTTGAAGTTTTGGTAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                         <  2:2251727/138‑1 (MQ=255)

                                                                                                 aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTATTTTTTGGAGATTTGAATGGCTTTTACGTTTTTTAAAAAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATATATCCAGTAAAATc                                         >  1:2935395/1‑140 (MQ=255)

                                                                                                 aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                         <  2:735081/140‑1 (MQ=255)

                                                                                                     gATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTAGTTTTTGGTGATTTGAATGGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatg                                     >  1:4180078/1‑140 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:612003/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:530106/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:1535198/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  2:2239491/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  2:1414549/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:4066597/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:3322919/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:1139567/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:2481433/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGATTTGGGAGATTTGCATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:768900/140‑1 (MQ=255)

                                                                                                       tCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  2:2370442/140‑1 (MQ=255)

                                                                                                       tCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGTGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAAAAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:324198/140‑1 (MQ=255)

                                                                                                       acagaccGACTATACGCGCCTTTTCGGATGTTTCTTTGAAGTTATGCGAGATTTGAATCGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  2:3306858/135‑1 (MQ=255)

                                                                                                       acATTCCGACTATACGGGCGTTTTCGTATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   <  1:3574237/139‑1 (MQ=255)

                                                                                                         aTTCCGACTATACGGGCGTTTTCGTATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgcatg                                 <  2:216270/140‑1 (MQ=255)

                                                                                                           tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                               <  1:486507/140‑1 (MQ=255)

                                                                                                           tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTCAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                               <  1:3178427/140‑1 (MQ=255)

                                                                                                            ccGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGGATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATgggg                              <  1:1225241/140‑1 (MQ=255)

                                                                                                               aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                   >  2:3232247/1‑132 (MQ=255)

                                                                                                                  ataCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattc                        <  2:525361/140‑1 (MQ=255)

                                                                                                                   taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGTGCTAATTGAATGGGATTTCCGTTTTTAAAAAAAAAGGCAAGCCAAAAAAAACCGACAACAACAAAAAATAATCTAGCAAGTAAAATCCATGCAAGGGGTaaataa                       >  2:2296394/1‑136 (MQ=255)

                                                                                                                   taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattaa                       >  2:263712/1‑138 (MQ=255)

                                                                                                                    aCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGTGATATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCAGACAACAACAAAAAATAATATAGCAAGTAAACACCAATCATGGGGTAagtcat                      >  2:1223918/1‑140 (MQ=255)

                                                                                                                        gCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                        >  2:3178394/1‑118 (MQ=255)

                                                                                                                           ttttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAAATAAATCGTTGc               >  2:3954219/1‑140 (MQ=255)

                                                                                                                            tttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCa              <  2:3985786/140‑1 (MQ=255)

                                                                                                                                  aTTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAatat        >  2:707522/1‑140 (MQ=255)

                                                                                                                                        tttttAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt  <  2:4206441/139‑1 (MQ=255)

                                                                                                                                            gAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                    >  1:861640/1‑86 (MQ=255)

GCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAT  >  NZ_CP034943/240050‑240325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`