Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,200,979 G→T 7.3% G200* (GGA→TGA)  EO946_RS16570 → spore germination protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,200,9790GT 7.3% 441.5 / 3.1 203G200* (GGA→TGA) EO946_RS16570spore germination protein
Reads supporting (aligned to +/- strand):  ref base G (78/102);  new base T (21/2);  total (99/104)
Fisher's exact test for biased strand distribution p-value = 8.97e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

AACGATAACGATTATTATTTTACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCC  >  NZ_CP034943/3200840‑3201118
                                                                                                                                           |                                                                                                                                           
aacgataacgatTATTATTTTACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAg                                                                                                                                             <  1:1330962/140‑1 (MQ=255)
aacgataacgatTATTATTTTACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAg                                                                                                                                             <  1:1003252/140‑1 (MQ=255)
  cgataacgatTATTATTTTACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTGCATCTCTTTTTTAGGa                                                                                                                                           >  2:719740/1‑140 (MQ=255)
  cgataacgatTATTATTTTACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGGTTTAGGTGAAGGTCTTGGACACATTGCTAACTCTCTTACTGTCGTTTCCCTCTCTTTTTTAGGa                                                                                                                                           >  2:4041325/1‑140 (MQ=255)
     taacgatTATTATTTTACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATg                                                                                                                                        <  1:2747936/140‑1 (MQ=255)
          attattattTGTCTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCTCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGt                                                                                                                                   <  2:2443419/140‑1 (MQ=255)
                   ttACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGtt                                                                                                                          <  2:2642561/140‑1 (MQ=255)
                      cTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATATGTTTTTTAGGAATTGAAGTGATTGTGtttat                                                                                                                       >  1:3646022/1‑138 (MQ=255)
                         aTTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTATGAATGGAAGTGATGCTGTTTCTTcc                                                                                                                    >  2:3139285/1‑140 (MQ=255)
                         aTTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTTATGCTGTTTATTcc                                                                                                                    >  1:2402637/1‑140 (MQ=255)
                         aTTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAACTGATGCTGTTTATTcc                                                                                                                    >  2:3213337/1‑140 (MQ=255)
                           tgtgTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATGGGTTTTTTAGGAATGGAACTGATGCTGTTTCTTCCTg                                                                                                                  >  1:3513023/1‑140 (MQ=255)
                           tgtgTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTACTg                                                                                                                  >  1:789023/1‑140 (MQ=255)
                           tgtgTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAAAGGAAGTGATGCTGTTTATTACTg                                                                                                                  >  1:2917545/1‑140 (MQ=255)
                           tgtgTTTGGGATCAGTATTAAAATTAGTGACATCAATCATGTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCGAGTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTg                                                                                                                  <  1:2363960/140‑1 (MQ=255)
                                   ggATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTTATGCTTTTTATTCCTGAACATATg                                                                                                          >  2:2516775/1‑140 (MQ=255)
                                   ggATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCGTTTTTATGAATGGAAGTGATGCTGTTTGTTCCTGAACATATg                                                                                                          >  1:400935/1‑140 (MQ=255)
                                         gTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGaagaaa                                                                                                    <  2:2789351/140‑1 (MQ=255)
                                                 agttttGACATCAATAATTTGCGCCCTGATTTAGGTGAAGGTCTTTGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTATTAATGGAAGTGATTCTGTTTCTTCCTGAACATATCTAGAAAATGAAATa                                                                                            <  2:2035729/138‑1 (MQ=255)
                                                 aTTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATa                                                                                            <  2:232655/140‑1 (MQ=255)
                                                 aTTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATa                                                                                            <  1:1569555/140‑1 (MQ=255)
                                                  tttttGCCATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCAGTGCTAACTCTCTGACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATac                                                                                           <  2:1226428/140‑1 (MQ=255)
                                                     ttGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATAcaca                                                                                        <  1:686430/140‑1 (MQ=255)
                                                       gACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACAtt                                                                                      <  2:2783638/140‑1 (MQ=255)
                                                       gACATCAATAAGTTGCGCCCTGTTTTCGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACAtt                                                                                      <  2:426882/140‑1 (MQ=255)
                                                        aCATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTc                                                                                     <  2:1561561/140‑1 (MQ=255)
                                                        aCATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACACATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTc                                                                                     <  2:2473788/140‑1 (MQ=255)
                                                          aTCAATAATTTGCTCACTGTTTTATGTGAAGGTCTTGGAACCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAg                                                                                   <  2:2396021/140‑1 (MQ=255)
                                                          aTCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAg                                                                                   <  2:2900308/140‑1 (MQ=255)
                                                          aTCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAg                                                                                   <  2:3802065/140‑1 (MQ=255)
                                                             aataatTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGata                                                                                <  2:883523/140‑1 (MQ=255)
                                                               taatTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATg                                                                              <  2:4233439/140‑1 (MQ=255)
                                                               taatTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATg                                                                              <  2:3093799/140‑1 (MQ=255)
                                                                    tGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTACATATCTTTTTTATGAAATGAAGTGATTCTGTTTGTTACTGAACAAAAGAAAAAAAAAAAAAACACAATCACATATTAGGCt                                                                         >  1:3304496/1‑140 (MQ=255)
                                                                      cgcCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGt                                                                       <  1:1552295/140‑1 (MQ=255)
                                                                         ccTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCgg                                                                    <  1:4266052/140‑1 (MQ=255)
                                                                          aggTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGc                                                                   <  1:3618328/138‑1 (MQ=255)
                                                                          aggTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGc                                                                   <  1:2015084/138‑1 (MQ=255)
                                                                            gTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTACATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAAAATATGAAGAAAAAGAAATACACATTCATATATTAGGCTGTAGGGtt                                                                 >  2:1727825/1‑140 (MQ=255)
                                                                             ttttAGGTGAGGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTttcttc                                                                                                                     <  1:1256649/87‑1 (MQ=255)
                                                                             ttttAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCAACTCTTTTTTATGAATTGAAGTTATGCTGTTTGTTACTGAACAAATGAAGAAAAATAAAAAAACAATCATATATGCGGCTGTCGGCgtt                                                                >  1:1724647/1‑140 (MQ=255)
                                                                                     gAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTACATCTCGTTTTTAGGAAAGGAAGTGATTCTGTTTATTCCTGAACAAATGAATAAAAAGAAAAAAACAATCAGATATTCGGCTGTAGGCGTTATGAttac                                                        >  1:57245/1‑138 (MQ=255)
                                                                                      aaGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATTGAAGTGATGCTGTTTATTACAGAAAATATTAAGAAAAATAAATAAAAAATAAGATATTCGGCTGTCGGCGTTCTGATTACg                                                       >  1:2792675/1‑140 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATGTCTTTTTTAGGAATGGAAGTGATGCTGTTTCGTCCTGAACATATGAAGAAAAAGAAATACACAGTCAGATATGCGGCTGTCGGCTTTGTGTTTGCGa                                                      >  2:187703/1‑140 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATGGCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACCTATGAAGAAAAAGAAATACAAATTCAGATATGGGGCTGTGGGGTTTCTGATTGCGa                                                      >  2:1424482/1‑140 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTTCACAAATGAAGAAAAAGAAATACACATTGAGATATTCGGCTGTGGGCTTTGTGATTGCGa                                                      >  1:3131068/1‑140 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTTTCGGCTTTATGATTACGa                                                      >  2:3406298/1‑140 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                      >  2:537964/1‑140 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                      <  2:112954/140‑1 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                      <  2:3603214/140‑1 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                      <  1:2081760/140‑1 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGa                                                      <  1:1385462/140‑1 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGAAGCAGTTTATTCCTTAACATATGAAGAAAAATAAATAAAAATTCAGATATGCGGCTGTCGGCTGTCTGATTCCGa                                                      >  2:194208/1‑140 (MQ=255)
                                                                                       aGGTCTTGGACCCATTGCTAACACTCTTACTGTCGTTTGGATCTGTTTTTTAGGTAAGGTCGTGATGCTGTTTGTTGCGGAACAGATGAAGCAAAAGAAATACAGATTCAGATATGCGGCTGTCGGCTGTGTGATTGCgg                                                      >  2:1264263/1‑139 (MQ=255)
                                                                                       aGGTCGTGGACCCATTGCTAACTCTCTTACTGTCGGTTCCATCTCTTTTTGAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATAGGCGGCTGTCGGCTTTCTGATTCCGa                                                      <  2:4071047/140‑1 (MQ=255)
                                                                                           cTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattat                                                  <  2:600282/140‑1 (MQ=255)
                                                                                           cTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGattat                                                  <  2:337603/140‑1 (MQ=255)
                                                                                              ggACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTGTTTTTTAGGAATGTAAGTGATGCGGTTTCTTCCTGAACATATGAAGAAAAAGAAATACAAATTAAGAGATGCGGCTGTGGGCTTTCTGATTCCGATTAtttt                                               >  2:3190901/1‑140 (MQ=255)
                                                                                              ggACCCATTGCTAACTCTCTTACTGTCGTGTCCATCTCTTTTTTAGGAAAGGAACTTATGCGGTTTCTTCCGGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGGTTTGTGATTCCGATTAtttt                                               >  2:3640390/1‑140 (MQ=255)
                                                                                               gACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTta                                              <  1:1989690/140‑1 (MQ=255)
                                                                                                aCCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTtat                                             <  2:260013/140‑1 (MQ=255)
                                                                                                aCCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTtat                                             <  2:1360676/140‑1 (MQ=255)
                                                                                                aCCCATTGCTAACTCTCTTACTGTCGTTTCAATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCAGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTtat                                             <  1:2063141/140‑1 (MQ=255)
                                                                                                    aTTGCTAACTCTCTTACTGTCGTTTCCCACTATTTTTTTGGTATGGTAGTGATGCGTTTTCTTGCAAAACATATTAAAAAAAAAAAATAAACAATAACAAATTAGGCTGTCGCCTTTCTGAATGCCATTATTTTataaat                                         >  1:1045634/1‑140 (MQ=255)
                                                                                                    aTTGCTAACTCTCTTACTGTCGTTTCCATCTGTTTTTTATGAATGGAAGCGATGCTGTTTGTTCCTGAAAATATGAATAAAAATAAATACACATTAAGATATTCGGCTGTCGGGGTTGTGATTACGATTATGTTatatat                                         >  1:453146/1‑140 (MQ=255)
                                                                                                    aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                         >  2:1005468/1‑140 (MQ=255)
                                                                                                    aTTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAAAATATGAATAAAAAGAAATAAACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTatatat                                         >  2:2073863/1‑140 (MQ=255)
                                                                                                    aTTGCTAACTCTCTTACTGTCGTTTACCTCTATTTTTTAGGAATGGAACTGATGCTGTTTCTTCCGGTACATATGCAGAAAAAAAAATACACATTCCGTAATGCGGCTGGCGGGGTTCTGATTCCGCTTATTTTatatat                                         >  1:2341342/1‑140 (MQ=255)
                                                                                                    aTTGCTAACTCTCTTACTGTCGTTTACATGTATTTTTTAGGAATTGAAGTGAAGCGGTTTCTTGCTGAAAATATGAAGAAAAACAAATACACAATCACGTATGGGGCTGTCGGGTTTGTGAATCCGATTATTTTatatat                                         >  2:569579/1‑140 (MQ=255)
                                                                                                     ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                        <  2:2661207/140‑1 (MQ=255)
                                                                                                     ttGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                        <  2:3709500/140‑1 (MQ=255)
                                                                                                     agacagaCTCTCTTACTGTCGTGTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATc                                        <  2:2959620/134‑1 (MQ=255)
                                                                                                           aCTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTGTTCCTGAACATATGTAGAAAAAGAAATACACATTCAGATATTCGGCGGTCGGGGTTGTGGTTGCGGTTATTTTATATATCCTGACa                                  >  1:3726727/1‑140 (MQ=255)
                                                                                                           aCTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACa                                  <  2:3048555/140‑1 (MQ=255)
                                                                                                           aCTCTCTTACTGTCGTTTACATGTCTTTTTTAGGAATTGAAGTGATGCTGTTTGTTTCTTAACATATTAAGAAAAATAAAAACACAATAAAATATTCGGCTGTGGGCGTTGTTATTGCGCTTATTTTatatataatgaaa                                  >  1:3260592/1‑133 (MQ=255)
                                                                                                            ctctctTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGTTGCTGTTTCTTCCGGAACATATTAAGAAAAAGAAATACAAAATCAGATATGCGGCTGTCGGCGTTCTGATTCCGAGTATTTTATATATACTGACat                                 >  1:3056239/1‑140 (MQ=255)
                                                                                                              ctctTACTGTCGTTTACATCTCTTTTTTATTAATGGAAGTGATGCTGTTTCTTCCTGAACTTATGATGAATAATATTTAAACTTTCATATATTCTGCTTTCGTGTTTTTTATTTTTATTATTTTTTATATCCTTAGatat                               <  2:825079/140‑1 (MQ=255)
                                                                                                                ctTACTGTCGTTTCCATCTCTTTTTTAGGCATGGAAGGGTTGCTGTTTCTTCCTGAACATATGAATAAAAAGAAAAACAAATTCACATATGCGGCTGTGGGCTTTCTGCTTCCGATTATTTTATATATCCGGAGCTATGt                             >  1:2992480/1‑140 (MQ=255)
                                                                                                                ctTACTGTCGTTTCCATCTCTTTTTTAGGAATTGAAGTGATGCTGTTTGTTCCTGAACATATGAAGAAAAAGAAATACACATTAAGATATGCGGCTGTGGGCGTTGTGATTGCGATTATTTTATATATACAGACATATGt                             >  1:2872299/1‑140 (MQ=255)
                                                                                                                    cTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATc                         <  1:1819651/140‑1 (MQ=255)
                                                                                                                       tCGTTTGCATCTCTTTTTTAGGAATGGAAGTGATGCTTTTTCTTCCTGAACATATGAAGAAAAAGAAATACACAATCAGATATTCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATACTGACATATTTCATCgtt                      >  2:3623990/1‑140 (MQ=255)
                                                                                                                       tCGTTTCCATCTCTTTTTTAGGAATTGAAGTGATGCTGTTTCTTCCTGAACATAAGAAGAAAAAGAAATACACATTAAGATATGCGGCTGTCGGAGTTGTTATTCCCATTATTTTATATATCCTTAAATATGTAATAgtt                      >  2:3454700/1‑140 (MQ=255)
                                                                                                                       tCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATTAAGAAAAAGAAATACACATTCAGATATTCGGCTGTCGGGTTTCTGATTCCGATTATTTTATATATCCTTACAAGTGTGATGgtt                      >  2:4160892/1‑140 (MQ=255)
                                                                                                                       tCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACATATTAAGAAAAAGAAATACAAATTCAGATATTCGGCTGTCGGCTTTGTGATTAGGATTATTTTATATTTCCTGTCATATTTCATCgtt                      >  2:3672800/1‑140 (MQ=255)
                                                                                                                       tCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTATTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTACGATTATTTTATATATACTGACATATGTCATCgtt                      >  2:941788/1‑140 (MQ=255)
                                                                                                                       tCGTTTCCATCGGTTTTTTAGGAATGGAAGTGATGCGGTTTCTTGCGGAACATATGAATAAAAAGAAATACAAATTCAGATATGCGGCTGTCGGCTTTGTGATTCCGATTATTTTATATATAATGACATATGTCATCgtt                      >  2:1501231/1‑140 (MQ=255)
                                                                                                                         gTTTCCATCTCTTTTTTATGAATGGAACTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTAGGGTTTCTGATTCCGATTATTTTATATATACTGACATATTTCATAgttgt                    >  2:1980263/1‑140 (MQ=255)
                                                                                                                         gTTTCCATCTCTTTTTTATGAATGGAACTGATGCTGTTTCTTCCTGAACAAATGAAGAAAAATAAATACACAATCAGATATGCGGCTGTGGGGTTTCTGATTCCGATTATTTTATATATCCTGACATATTTCAACgttgt                    >  2:1833236/1‑140 (MQ=255)
                                                                                                                         gTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAAAATATTAAGAAAAAGAAATAAACATTCAGATATTCGGCTGTCGGCTTTCTGATTACGATTATTTTATATATACTGACATAATTAATAgttgt                    >  2:3306393/1‑140 (MQ=255)
                                                                                                                         gTTTCCATCTCTTTTTTAGGAATGGAAATGATGCTGTTTCTTACTGAACATATGAAGAAAAAGAAATACACATTAAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgt                    >  2:392527/1‑140 (MQ=255)
                                                                                                                         gTTTCCATCTCTTTTTTAGGAAGGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATAAACATTCAGATATGGGGCTGTCGGCTTTCTGATTCCGAGTATTTTATATATCCTGACATATGTCATCgttgt                    >  2:2562571/1‑140 (MQ=255)
                                                                                                                         gTTTCCAACTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTTATTCCCATTATTTTATATATCCTGACAAATTTCATAgtttt                    >  2:4000225/1‑138 (MQ=255)
                                                                                                                          tttCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgtt                   <  1:1856632/140‑1 (MQ=255)
                                                                                                                          gttCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCgttgtt                   <  2:1517361/139‑1 (MQ=255)
                                                                                                                                    agagacaGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGa         <  1:3411263/134‑1 (MQ=255)
                                                                                                                                     tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc        <  2:513182/140‑1 (MQ=255)
                                                                                                                                     tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc        <  1:2829581/140‑1 (MQ=255)
                                                                                                                                     tttttAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc        <  1:1014392/140‑1 (MQ=255)
                                                                                                                                     tttttAGGAATGGAAGCGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGAc        <  2:453146/140‑1 (MQ=255)
                                                                                                                                           ggAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACCTATGTCATCGTTGTTGGGGCTTTGACCGCTcc  <  1:861020/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AACGATAACGATTATTATTTTACTGATTGTGTTTGGGATCAGTTTTAAAATTTTTGACATCAATAATTTGCGCCCTGTTTTAGGTGAAGGTCTTGGACCCATTGCTAACTCTCTTACTGTCGTTTCCATCTCTTTTTTAGGAATGGAAGTGATGCTGTTTCTTCCTGAACATATGAAGAAAAAGAAATACACATTCAGATATGCGGCTGTCGGCTTTCTGATTCCGATTATTTTATATATCCTGACATATGTCATCGTTGTTGGGGCTTTGACCGCTCC  >  NZ_CP034943/3200840‑3201118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: