Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,268,712 G→T 5.2% intergenic (+60/+150) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,268,7120GT 5.2% 657.2 / 4.8 233intergenic (+60/+150)catR/EO946_RS16920catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (36/176);  new base T (18/2);  total (54/179)
Fisher's exact test for biased strand distribution p-value = 4.32e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

AGGAAAGGCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGAT  >  NZ_CP034943/3268574‑3268849
                                                                                                                                          |                                                                                                                                         
aggaaaggCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGc                                                                                                                                          <  1:4239826/140‑1 (MQ=255)
 ggaaaggCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGcc                                                                                                                                         <  2:522368/140‑1 (MQ=255)
    aaggCGTTGGCCCCCCTCTTGGGTGAGATTTCTAAATGGGCGACTGATTGGATTGACCCTTCCTTGCTTGACTAAGTGCTAAAACGGTTCTGATCTCAAAGGCGTGAAAAGATCCGTTTTTTTTAGTTTCATTTGCCAgg                                                                                                                                      >  2:2448325/1‑140 (MQ=255)
    aaggCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAgg                                                                                                                                      >  2:298651/1‑140 (MQ=255)
       gCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGTaa                                                                                                                                   >  2:385660/1‑140 (MQ=255)
       gCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTaa                                                                                                                                   <  1:1018094/140‑1 (MQ=255)
          ttGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTaaaaa                                                                                                                                >  2:751392/1‑140 (MQ=255)
                    tCTTGGTTGTGTTTTCTAATTGGGCGAGGGATTGGATTGACCCTTCCTTTCTTGAATAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                      <  1:3381612/140‑1 (MQ=255)
                    tCTTGGGTGATTTTTCTAAATGGGCGTCGGATTGGATTGACCCTTCCTTTCTTTACTATTTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                      <  1:840858/140‑1 (MQ=255)
                    tCTTGGGTGAGTTTTCTAATTGGGCGACGGATTGGATTGACCCTTCCTTTGTTGACAAGTTGCTAAAACGGTTCTGATCTCAAAGTCGAGAAAATAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                      <  1:3109421/140‑1 (MQ=255)
                    tCTTGGGTGAGATTTCTAATTGGGCGAGGGATTGGATTGACCCTTCCTTTCTTGAATAATTGCTAAAACGGTTCTGATCTCAACGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                      <  1:584898/140‑1 (MQ=255)
                    tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                      <  1:3979312/140‑1 (MQ=255)
                    tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                      <  2:1407753/140‑1 (MQ=255)
                    tCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTtctc                                                                                                                      <  1:2987383/140‑1 (MQ=255)
                      ttGGGTGAGATTTCTAACTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAg                                                                                                                    <  1:2536005/140‑1 (MQ=255)
                      ttGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAACGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAg                                                                                                                    <  2:2184897/140‑1 (MQ=255)
                       tGGGTGATTTTTCTAATTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCGAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  1:1784426/140‑1 (MQ=255)
                       tGGGTGATATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:2665367/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGTATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:1618056/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGATAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTCAAAAACGACTTCTCAGc                                                                                                                   <  2:2112576/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAgt                                                                                                                   <  2:1970072/140‑2 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  1:1023464/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:4098371/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:909728/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  1:761037/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:1712736/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:1829093/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:862564/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:636913/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:3399412/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  1:3392127/140‑1 (MQ=255)
                       tGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  1:3345876/140‑1 (MQ=255)
                       tGGGTGAGATCTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  2:552035/140‑1 (MQ=255)
                       tGGGAGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGc                                                                                                                   <  1:3002993/140‑1 (MQ=255)
                         ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                 <  1:1387849/140‑1 (MQ=255)
                         ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                 <  1:1107607/140‑1 (MQ=255)
                         ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCg                                                                                                                 <  1:4060793/140‑1 (MQ=255)
                         ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGGAAAAAACGACTTCTCAGCCg                                                                                                                 >  2:1163405/1‑140 (MQ=255)
                         ggTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTACTTTCTTTACTATTTGCTAAAACGGTTATTATCTCAAAGGCGAGTAAAGAGCCTTTTTTTTTTTTTTGATTTTTAATGTAATATACGACTtctcttgag                                                                                                                 <  2:2042497/140‑6 (MQ=255)
                            gtgaTTTCTAATTGGGCGACGGATTGTATTGACCCTTCGTTTCTTGACTAATTGCTAAAACGGTTCTTATCTCAAAGGCGATAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                              <  1:2429710/138‑1 (MQ=255)
                            gagtTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                              <  1:906021/140‑1 (MQ=255)
                            gagaTTTCTAATTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                              <  1:2950555/140‑1 (MQ=255)
                            gagaTTTCTAAATGGGCGACTGATTGGATTGAACCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCTTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                              <  2:2277311/140‑1 (MQ=255)
                            gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                              <  1:1349729/140‑1 (MQ=255)
                            gagaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGata                                                                                                              <  1:3184681/140‑1 (MQ=255)
                              gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                            <  2:1628886/140‑1 (MQ=255)
                              gaTTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGatata                                                                                                            <  1:667562/140‑1 (MQ=255)
                                   cTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATAtagta                                                                                                       <  2:3654827/140‑1 (MQ=255)
                                     aactGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAAGTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGa                                                                                                     <  2:285308/137‑1 (MQ=255)
                                     aaaTGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGa                                                                                                     <  2:3269889/140‑1 (MQ=255)
                                                         tgACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAg                                                                                 <  1:200206/140‑1 (MQ=255)
                                                         tgACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTAAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGAATTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAg                                                                                 <  1:1175027/140‑1 (MQ=255)
                                                          gACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGc                                                                                >  2:302102/1‑140 (MQ=255)
                                                             ccttccttTCTTGACTAATGGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACCTTGCGTTTTTAGCCga                                                                             <  2:4057745/140‑1 (MQ=255)
                                                             ccttacttTCTTGAAGAATCGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGGAGAACCCCACATTGCGTTTTTAGCCga                                                                             <  2:2378805/140‑1 (MQ=255)
                                                               ttccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGCGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCgaag                                                                           <  1:3663595/140‑1 (MQ=255)
                                                                tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTTCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCcgacga                                                                          >  2:3148390/1‑140 (MQ=255)
                                                                tccttTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTTCCAGGTAAAAAACGACTTATCAGCCGATATATATTAGAACCCCACAATGCGTTTTTAGCCgaaga                                                                          >  2:418043/1‑140 (MQ=255)
                                                                     tCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTAATTTGGCACGTAAAAAACGACTTATCAGCCGATATATAGTAGAAACACACATTTCGTTTTTATCCGAATTAtttt                                                                     >  1:2060582/1‑139 (MQ=255)
                                                                     tCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTGTTTTATTTTTAATTTGCCACGTAAAAAACGAATTGTCACCCGATATATATTATAACACCACAATGCGTTTTTATCCCAAGAAattt                                                                     >  1:4523/1‑139 (MQ=255)
                                                                       ttGACTAATTGCTAAAACGGTTCTTATATCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGag                                                                   <  2:3357643/140‑1 (MQ=255)
                                                                       ttGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGag                                                                   <  1:2715676/140‑1 (MQ=255)
                                                                       ttGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGag                                                                   <  1:129592/140‑1 (MQ=255)
                                                                       ttGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGag                                                                   <  1:1134100/140‑1 (MQ=255)
                                                                       ttGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTGTTTTTTTTTTTAATTTGCCACGTAAAAAAAGACATGTCAGCCGATATATAGTATAAACCCCCAATGCGGTTTTTTCCGAACAAATTTag                                                                   >  1:50124/1‑140 (MQ=255)
                                                                         gACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTGATTTGCCAGGTAAAAAAAGACTTATAAGCCGATATATAGTAGAAACCCACATTGCGTTTTTAGGCCAACAATTTgtgga                                                                 >  2:2570040/1‑136 (MQ=255)
                                                                         gACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCGGCCGGTAAATAGTAGAAACCCACATTGCGTTTTTATCCGAAGAATTTGagca                                                                 >  2:787896/1‑140 (MQ=255)
                                                                              aTTGCTAAAACGGTTCTGATCTCTAAGGCGCGTAAAGAGCCGTGTTTTTATTTTTGATTTGGGCGGGGCAAAAAGTCGTGGCAGCCGCTATATAGTTGTACCCCCCACTGAGTTTTTATCCGAAGCATTTGAGCAGCTgg                                                            >  1:3577802/1‑140 (MQ=255)
                                                                                  cTAAAACGGTTCTGATCTCACAGGCGAGAAAAGAGCCGTTTTTTTATTATACATTTGCCAGGTAAAAAACGACTTCACAGCCGATATATAGTAGAACCCCACAGTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGaa                                                        <  2:2295684/140‑1 (MQ=255)
                                                                                  cTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGACGAATTTCAGCAGCTGGCGaa                                                        <  2:179270/140‑1 (MQ=255)
                                                                                  cTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCAGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGaa                                                        <  2:3516916/140‑1 (MQ=255)
                                                                                         ggTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                 <  2:608188/140‑1 (MQ=255)
                                                                                         ggTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                 <  2:2894203/140‑1 (MQ=255)
                                                                                         ggTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCtt                                                 <  2:2803901/140‑1 (MQ=255)
                                                                                           ttCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCa                                               <  1:2046180/140‑1 (MQ=255)
                                                                                              tgctctcCAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCCTTTGCCAGGTAAACAACGCCTTCGCAGCCGATATATCGGAGCACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATga                                            <  2:3956174/137‑1 (MQ=255)
                                                                                                   tcAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGagaaga                                       <  2:1559902/140‑1 (MQ=255)
                                                                                                        ggCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                  <  1:2299446/140‑1 (MQ=255)
                                                                                                        ggCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGAt                                  <  1:3036708/140‑1 (MQ=255)
                                                                                                             gaAAAGAGCCGTTTTTTTATTTTTGATTTGCCACGTAAAAAAAGACATCTCACCCGATATATAGTATAACCCCACAATTGGTTTTTAGCCGCAGAATTTGAGCAGCTGGCGCACGAGCTTCATGAGAAGATTGATCAGtt                             >  2:3537863/1‑140 (MQ=255)
                                                                                                             gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                             <  1:1652872/140‑1 (MQ=255)
                                                                                                             gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                             <  2:2334038/140‑1 (MQ=255)
                                                                                                             gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                             <  2:461818/140‑1 (MQ=255)
                                                                                                             gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                             <  2:3577802/140‑1 (MQ=255)
                                                                                                             gaAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGtt                             <  1:3608322/140‑1 (MQ=255)
                                                                                                              aaaaGAGCCGGTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGACGATATATAGTAGAACCCCACATTGAGTTTTTATCCGAAGAATTTTAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTc                            <  2:2524867/140‑1 (MQ=255)
                                                                                                              aaaaCAGCCGTGTTTTTCTTTTTCAATTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGGTc                            <  2:2402048/140‑1 (MQ=255)
                                                                                                                             ttATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc             <  1:1880544/140‑1 (MQ=255)
                                                                                                                             ttATTTTTCATTTGCCAGGTAAAAAACGACTTCCCAGCCGATATATAGTACCACCCCCCATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc             <  1:1050893/140‑1 (MQ=255)
                                                                                                                             ttATTTTTCATGTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAAcc             <  1:385660/140‑1 (MQ=255)
                                                                                                                                 ttttCATTTGCCAGGTAAAAAACGACTTCTCAGACGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACg         <  2:1033205/140‑1 (MQ=255)
                                                                                                                                  tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt        >  1:922358/1‑140 (MQ=255)
                                                                                                                                  tttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGt        <  1:2974807/140‑1 (MQ=255)
                                                                                                                                   ttCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTc       <  1:3561484/140‑1 (MQ=255)
                                                                                                                                       tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa   <  1:2347050/140‑1 (MQ=255)
                                                                                                                                       tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa   <  1:734923/140‑1 (MQ=255)
                                                                                                                                       tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa   <  1:377298/140‑1 (MQ=255)
                                                                                                                                       tttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGa   <  1:1078484/140‑1 (MQ=255)
                                                                                                                                        ttGCCAGGTAAAACACGACTTCTCAGCCGATAGATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGAt  <  2:1253303/140‑1 (MQ=255)
                                                                                                                                        ttGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGATCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGAt  <  2:1426860/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
AGGAAAGGCGTTGGCCCCCATCTTGGGTGAGATTTCTAAATGGGCGACGGATTGGATTGACCCTTCCTTTCTTGACTAATTGCTAAAACGGTTCTGATCTCAAAGGCGAGAAAAGAGCCGTTTTTTTATTTTTCATTTGCCAGGTAAAAAACGACTTCTCAGCCGATATATAGTAGAACCCCACATTGCGTTTTTAGCCGAAGAATTTGAGCAGCTGGCGAAAGAGCTTCATGAGAAGATGGATCAGTTCACATTTAAAATAACCCACGTCCAGAT  >  NZ_CP034943/3268574‑3268849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: