BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,280,377	G→T	9.2%	Q115K (CAA→AAA)	EO946_RS16965 ←	lantibiotic dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,280,377	0	G	T	9.2%	722.4 / 8.6	306	Q115K (CAA→AAA)	EO946_RS16965	lantibiotic dehydratase
Reads supporting (aligned to +/- strand): ref base G (177/78); new base T (3/44); total (180/126)
Fisher's exact test for biased strand distribution p-value = 1.01e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

AGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTTTT  >  NZ_CP034943/3280239‑3280516

aGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTAAATGTAAAAGAAAGTCTGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGTTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGa                                                                                                                                            >  1:1511541/1‑140 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACACGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                           <  1:662369/140‑1 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGAt                                                                                                                                           >  2:317806/1‑140 (MQ=255)

 gTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTGTTGTAAAATGAATGTTGAt                                                                                                                                           >  1:138164/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                         >  2:3434257/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                         >  2:1270952/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                         >  1:2258452/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                         >  1:2583952/1‑140 (MQ=255)

   aaGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATGTTCTAATTAATTGGTAGAGCCACTCAAAAGCAACACGAGCCTTTTTGTAAAATGAATGTTGATTa                                                                                                                                         >  1:3349659/1‑140 (MQ=255)

      cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                                                      >  2:4079995/1‑140 (MQ=255)

      cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                                                      >  1:2211568/1‑140 (MQ=255)

      cccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                                                      >  1:3130094/1‑140 (MQ=255)

      cccGGCCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTaaaa                                                                                                                                      >  2:3542764/1‑140 (MQ=255)

       ccGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTGCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAg                                                                                                                                     <  1:4216963/140‑1 (MQ=255)

        cGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGa                                                                                                                                    >  2:841057/1‑140 (MQ=255)

         ggTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGAt                                                                                                                                   >  1:1228292/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                  >  2:3970639/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                  >  2:2042106/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                  >  2:1613126/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                  >  2:1726805/1‑140 (MQ=255)

          gTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATa                                                                                                                                  >  1:2509622/1‑140 (MQ=255)

            cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                                                                >  1:31676/1‑140 (MQ=255)

            cACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAg                                                                                                                                <  1:2785276/140‑1 (MQ=255)

                      ataACAAGCTGAATTTAATGTAAAATAAAGTCGGTGGGTTTTTTCGTTTTCAAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCATTTTTGTAAAATGATTGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                      <  1:3898938/140‑1 (MQ=255)

                      ataACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTAt                                                                                                                      <  2:4176392/140‑1 (MQ=255)

                        aaCAAGCGGAATTTACTGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTACATTTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                                                                    <  2:2570383/140‑1 (MQ=255)

                        aaCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAACGATAAGTGATTTTTATCa                                                                                                                    <  2:3949090/140‑1 (MQ=255)

                        aaCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCa                                                                                                                    <  2:2016230/140‑1 (MQ=255)

                         aCATGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATGTTCTAATTAATTGGTAGAGCCACTCAAATTAAACACTTGCCTTTTTGTAATATGAATGTTGATTAAAAGTTAAGTGATTTTTATCAg                                                                                                                   >  2:2467458/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCGTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  1:212402/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGACTGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  2:2628958/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCat                                                                                                                   >  2:1664748/1‑139 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  2:1202891/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  1:1038128/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  2:1217419/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  1:1820736/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  2:2732269/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  2:2858798/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  1:3556639/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  1:3215672/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  1:3257895/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  2:4248463/1‑140 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATAAAAACGAGCCTTTTTGTAAAATGAATGTTGATTACAAGATAAGTGAttttgttaag                                                                                                                   >  1:2311888/1‑134 (MQ=255)

                         aCAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAAGTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAAGGTTGATTAAAAGATAAGTGATTTTTATCAg                                                                                                                   >  2:2377820/1‑140 (MQ=255)

                             gcgtattttaatttaaATTAATGTCGGTGGGTGTATTCTTTTTCAAATTTTCTAATTATTTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATTAATGTTTATTAAAAGATAAGTTATTTTTATCATaaaa                                                                                                               <  2:1576317/134‑1 (MQ=255)

                             gcgtattttAATGTAAAATAAAGTGGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTAAAAATCAACCCGCCCCTTTTTTTAAAATGATTTTTTATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                               <  2:1870553/134‑1 (MQ=255)

                             gcgtattttAATGTAAAAGAAAGTGGGTGTGTGTATTCGTTTTACAATTTTATATTTATTTGGTAGAGCCACTAAAAATCAACACGAGCGTTTTTGTAAAATGATTGTTGTTTAAAAGATAAGTGATTTTTATCTGaaaa                                                                                                               <  2:14756/134‑1 (MQ=255)

                             gcgcAATTTAATGTAAAAGAAAGTGGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGTTTGAGCCACTAAAATCCAACCCGCGCCTTTTTGTAAAATGATTTTTGTTTAAAAGATAAGTGATTTTTATCAGaaca                                                                                                               <  2:1994304/140‑3 (MQ=255)

                             gCGTAATTTATTTTAAAAGAAAGTCGGTCGGTTTATTCGTTTTACAATTTTCTAATTAATTGGTAGAGCAAATAAAAATCAACACTAGCGTTTTTTTAAAATGATTGTTTATTAAAAGATACTTGATTTTTATCATaaaa                                                                                                               <  2:3734264/140‑1 (MQ=255)

                             gCGTAATTTATTGTAAAATAAAGTGGGTGGTTTTATTATTTTTCCATTTTTCTAATTATTTGTTAACGCCAAAAAAAATAAACACGAGCCTTTTTGTAAAATGATTTTTTATTAAATCATCAGTTATTTTTAGCATaaaa                                                                                                               <  2:1408132/140‑1 (MQ=255)

                             gCGTAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCAATAAAAATCAACACGAGCCTTTTTTTAAAATGAATGTTGATTAAAAGATAAGTTATTTTTATCAGaaaa                                                                                                               <  2:2656584/140‑1 (MQ=255)

                             gCGGATTTTAATTTAAAAGAAAGTCGGTCGGTTTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTTTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaca                                                                                                               <  2:327552/140‑3 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTATAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                               >  2:3253419/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAATATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaca                                                                                                               >  1:1886048/1‑138 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                               >  2:2696474/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                               >  2:1937385/1‑140 (MQ=255)

                             gCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGaaaa                                                                                                               >  2:729881/1‑140 (MQ=255)

                               ggAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACg                                                                                                             >  1:3745483/1‑140 (MQ=255)

                                 aaTTTAATGTAACAGAACGTCGGTCTGTGTTTGCGTTTTCCATTTTTCTAATTTATTGGTAGCGCCACTCAAAATCAACCCGCGCCTTTTTGTAAAATGAATTTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGAc                                                                                                           <  2:2423702/140‑1 (MQ=255)

                                 aaTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGAc                                                                                                           >  1:3653209/1‑140 (MQ=255)

                                  aTTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGAcc                                                                                                          >  1:1287065/1‑140 (MQ=255)

                                        tGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATAc                                                                                                    >  2:1530746/1‑140 (MQ=255)

                                           aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                                                                 >  2:3135843/1‑140 (MQ=255)

                                           aaaaGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGa                                                                                                 >  1:915985/1‑140 (MQ=255)

                                                  agTCGGTCGGTGTATTCGTTTTCCAATTTTCTCATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTTATTAAAAGATAAGTGATTTTTATAAGAAAACGACCGTATACCGACTgaaga                                                                                          >  2:3489298/1‑140 (MQ=255)

                                                  agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAAGGACCCTATACCGACTgaata                                                                                          >  2:2774829/1‑138 (MQ=255)

                                                  agTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAAAACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTgaaga                                                                                          >  2:280103/1‑140 (MQ=255)

                                                    tcggtcggTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGTTAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGaaa                                                                                        >  2:3867006/1‑140 (MQ=255)

                                                           gtgtATCCGTTTTCCAATTTTCTAATTATTTGGTAGAGCCACTAAAAACCAAAACGAGCCTTTTTTTAAAAGGATTTTTTATTAAAAGATAAGTCATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGcc                                                                                 <  2:3809947/140‑1 (MQ=255)

                                                            tgtATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCa                                                                                >  2:3379963/1‑140 (MQ=255)

                                                             tttttgtttttCAATTTTTTTAATTATTTGTTAGCGAAAATAAAAATCAACTCGTGATTTTTTTTAAATTTATTTTTTATTAAAATATAATTTTTTTTTATAATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaa                                                                               <  1:586111/133‑1 (MQ=255)

                                                              ttttCTTTTTCAAATTTTATAATTATTGGGTCGCGCCACAAAAAATCAACACTCGCTTTTTTTTAAAATTATTGTTGATTAAATGATCAGTTATTTTTATCATAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                              <  1:4079342/138‑1 (MQ=255)

                                                              ttttCTTTTTAAAATTTTCTAATTAATTGGTAGAGCCAATCAAAATCAACACGACCATTTTTGTAAATTTATTGTTTATTAAAATATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCaaa                                                                              <  1:2949494/138‑1 (MQ=255)

                                                                      ttCCAATTTTTTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                      >  1:4032549/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGTATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                      >  1:3023379/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCACAAGGTGTgg                                                                      >  2:6115/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                      >  2:2440075/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                      >  2:528746/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                      >  2:1068697/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                      >  2:1105226/1‑140 (MQ=255)

                                                                      ttCCAATTTTCTAATTAATGGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTgg                                                                      >  2:3587414/1‑140 (MQ=255)

                                                                       tCCAATTTTCTAATTAATTGGTAGAGCAAATCAAAATCAACACTAGCCTTTTTTTAAAATTAATGTTTATTAAAATATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGt                                                                     <  2:2258452/140‑1 (MQ=255)

                                                                       tCCAATTGTCTAATTAATGGGTAGAGCCACACAAAATCAACCCGAGCCTTTTTGTAAAATGAATGTTTAATAAAAGATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGt                                                                     <  2:1980666/140‑1 (MQ=255)

                                                                          aaTTTCCTAATAAATTGGTACCCCCAAACAACAACACCCCTAGCATTTTTGTAAAATCAATGTTTAATAACAGATAAGTGATTTATATAACAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCt                                                                  <  1:1659856/140‑1 (MQ=255)

                                                                                   attaattGGTAGAGCCACTCAAAATCCACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGctct                                                         <  2:3080568/140‑1 (MQ=255)

                                                                                        ttGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCttg                                                    >  2:2218207/1‑140 (MQ=255)

                                                                                             agagCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGATTGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                               <  2:4034979/140‑1 (MQ=255)

                                                                                             agagCCAAACAAAATAAACACGACCCTTTTTTTAAAATTAATGTTGATTAAAAGATACCTCATTTTTAAAAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                               <  1:2838313/140‑1 (MQ=255)

                                                                                             agacCCACACAAAATAACCCCCAGACTTTTTTTAAAATGAATTTTTATTAAAAGATAAGTTATTTTTATCACAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCa                                               <  1:513525/140‑1 (MQ=255)

                                                                                               cgCCACTAAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTTATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATa                                             <  2:297549/139‑1 (MQ=255)

                                                                                                 ccACTCAAAATAAACACGAGCCTTTTTGTAAAATTAATGTTTATTAAAAGATAAGTGATTTTTATAAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACt                                           <  2:1317617/140‑1 (MQ=255)

                                                                                                    cTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCa                                        >  2:477499/1‑140 (MQ=255)

                                                                                                      cAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCaaa                                      >  1:3443165/1‑140 (MQ=255)

                                                                                                           acAACACGAGCTTTTTTTTAAATTTAATGTTTATTAAAAGATACGTTATTTTTAACAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                                 <  1:1937385/139‑1 (MQ=255)

                                                                                                           aaaaCACGCTCTTTTTTTTAAATTTATTGTTTTTTAAAATATATGTTATTTTTATAATAACACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGc                                 <  1:356886/138‑1 (MQ=255)

                                                                                                              acacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTGTTGTTGCTGTTGTTGTTGCATACTTCAAAATAGCCTg                              >  2:2160618/1‑140 (MQ=255)

                                                                                                              acacGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTTTTGCTCTTCTTGTTGCATACTTCACAATAGCCTg                              >  2:3565938/1‑140 (MQ=255)

                                                                                                                   aGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGaa                         >  1:766108/1‑140 (MQ=255)

                                                                                                                                   gaatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtttttttt         <  2:1886048/140‑1 (MQ=255)

                                                                                                                                   gaatgTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTAGACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCtttttttt         <  2:3697974/140‑1 (MQ=255)

AGTAAGCCCGGTCACCCTTAATATAACAAGCGGAATTTAATGTAAAAGAAAGTCGGTCGGTGTATTCGTTTTCCAATTTTCTAATTAATTGGTAGAGCCACTCAAAATCAACACGAGCCTTTTTGTAAAATGAATGTTGATTAAAAGATAAGTGATTTTTATCAGAAAACGACCCTATACCGACTGAAGAAAAAAGGCCAAAAGGTGTGGTTCTTGTTGCTCTTCTTGTTGCATACTTCAAAATAGCCTGTTGAAAGTTGCGCTTTTTTTTCCCTTTT  >  NZ_CP034943/3280239‑3280516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`