BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,211	G→T	9.3%	A107A (GCC→GCA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,211	0	G	T	9.3%	646.3 / 7.7	285	A107A (GCC→GCA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (100/144); new base T (38/2); total (138/147)
Fisher's exact test for biased strand distribution p-value = 2.38e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

AGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240075‑240345

agagagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATAAGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATGGCt                                                                                                                                     >  2:872236/1‑140 (MQ=255)

  agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATGGCttt                                                                                                                                   >  1:927904/1‑140 (MQ=255)

   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAATTTTTTTTGAAATTTTGGGAGATTTTAATGGCtttt                                                                                                                                  >  1:3963105/1‑140 (MQ=255)

    agagaAGTAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATGGGTTTTc                                                                                                                                 >  2:628803/1‑140 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACTGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTc                                                                                                                                 >  2:2730181/1‑140 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGTTTTGAATGGCTTTTc                                                                                                                                 >  1:3009855/1‑140 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                                 >  2:162627/1‑140 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAGTTTTGTGAGATTTGAATGGCtttta                                                                                                                                 >  2:3343675/1‑139 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCttttg                                                                                                                                 >  2:1838124/1‑139 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGGttttg                                                                                                                                 >  1:1025251/1‑139 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                 >  2:1134630/1‑140 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                                 >  2:2047925/1‑140 (MQ=255)

    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGAGGCTGTTTTTGTATTTTTGTTCTAATACTAAGTCGATAATTACGACTATACGGGCGTTTTGGGATTTTTTTTTGTAGTTTTGTGATATTTGAATGTCTGTTc                                                                                                                                 >  1:2003967/1‑140 (MQ=255)

      agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAATGCTTTTCCg                                                                                                                               >  2:1816846/1‑140 (MQ=255)

          ggAGCATCTCTGATGTTGGAATTATTCAGAGATGCGGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTATGATTTTTGTTTGAAGTTTTGTGAGATTTTTATTGCTTTTCCGtttt                                                                                                                           >  2:1740349/1‑140 (MQ=255)

            aGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTa                                                                                                                         >  2:3215922/1‑140 (MQ=255)

              cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGCTTTTGCGTTTTTaaa                                                                                                                       >  1:3760479/1‑140 (MQ=255)

                 ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                    <  1:3044715/140‑1 (MQ=255)

                 ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                                    <  2:350665/140‑1 (MQ=255)

                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGCTATTTGTATGGCGTTTACGTTTTTAAAACAAGAgg                                                                                                               >  1:630088/1‑140 (MQ=255)

                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAACAAGAgg                                                                                                               >  2:2651557/1‑140 (MQ=255)

                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGTGATTTTAATTTGTTTTACGTTTTTAAAACAAGAgg                                                                                                               >  1:729136/1‑140 (MQ=255)

                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                               >  2:3928532/1‑140 (MQ=255)

                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGAATTTTGTTTGAATTTTTGGGAGATTTGAATGGCGTTTACGTTTTTAAAACAAGAgg                                                                                                               >  1:3135552/1‑140 (MQ=255)

                      aTGTTGGAATGATTCAGAGATGCTGTTTTTGTATTTGTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                               <  2:326/140‑1 (MQ=255)

                        gTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGGATGGCTTTTCCGTTTTTAAAACAAGAGGCa                                                                                                             >  2:1225241/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTGTATGGCTTTTGCGTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  1:512799/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  2:3576637/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGGGATTTGAATGTCTTTTACGTTTTTAAAAAAAGATGCAAGc                                                                                                          >  1:2492665/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                          >  2:3851242/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  2:726671/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGTTTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGc                                                                                                          >  2:944374/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAga                                                                                                          >  2:3321067/1‑139 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                          >  1:425725/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                                          >  2:3119338/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGATGCaatc                                                                                                          >  2:1426708/1‑138 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTGGAAGTTTTGGGAGAATTTAATTGCTTTTACGTTTTTAAAAAAAGaagcaagc                                                                                                          >  2:308830/1‑140 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAAATGGTTTTACCTTTTTAAAACAAGAGTCaatc                                                                                                          >  1:4075674/1‑138 (MQ=255)

                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTGAAGTTTTTGGAGATTTTTATTGCTTTTACGTTTTTAAAACAAGACGCAAGc                                                                                                          >  1:139073/1‑140 (MQ=255)

                            gAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGCGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGcc                                                                                                         >  2:1371179/1‑140 (MQ=255)

                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                    <  2:1550531/140‑1 (MQ=255)

                                 agcacgagaTGCTGTTTGTGTTGTTTTTTTCTACTACGAATTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGATGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                                    <  1:2836100/135‑1 (MQ=255)

                                  ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTAAGTTTTTGGATATTTTAATTGGTTTTACGTTTTTTAAAGAATAGGCAAGCCAGATaa                                                                                                   >  1:3567941/1‑140 (MQ=255)

                                  ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATaa                                                                                                   <  1:118885/140‑1 (MQ=255)

                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTTAGTTTTTGGAGAGTTGAATTGGTTTTGCGTTTTTAAAACAATAGGCCAGACCGATAAACCCg                                                                                              >  1:3372444/1‑140 (MQ=255)

                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGacaac                                                                                         >  2:2414527/1‑140 (MQ=255)

                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGTATTTTTTTTTAATTGTTGGGTGATTTTTATTTCTTTTGCGTTTTTTAAACAAGATGAAATCCAGATAAAAGCGaaaac                                                                                         >  1:1854390/1‑140 (MQ=255)

                                            cTGTTTTTGTATGTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTAGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaac                                                                                         <  1:745802/140‑1 (MQ=255)

                                               tttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGAAAAACCCGacaacaac                                                                                      >  2:2896439/1‑140 (MQ=255)

                                               tttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATAAGGGCGTTTTCGGATTTTTTTTTGTAGTTTTTGGCGATTTTAATTGGTTTTACGTTTTTTAAACAAAAGGCAAACCAGATAAAACCCaccacaac                                                                                      >  1:1653862/1‑140 (MQ=255)

                                                      aTTTTTGTTCTAATACGAAGTCGATCAGTCCGACTATACGGGTGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaat                                                                               <  1:4063522/140‑1 (MQ=255)

                                                           tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGTATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                          <  2:3785379/140‑1 (MQ=255)

                                                           tGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                          <  2:1012421/140‑1 (MQ=255)

                                                            gTTCTAATACGAAGTCAATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTa                                                                         <  2:3054855/140‑1 (MQ=255)

                                                             ttgtAATACGAAGTCGATCATTCCGACTATACGGGCGTTTGCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTATTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  1:2730181/137‑1 (MQ=255)

                                                             ttCTCAGACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGATTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  1:2361258/140‑1 (MQ=255)

                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  1:3495959/140‑1 (MQ=255)

                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  2:2604578/140‑1 (MQ=255)

                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                        <  2:1485126/140‑1 (MQ=255)

                                                                  aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                                   <  2:3372444/140‑1 (MQ=255)

                                                                   tACGCAGTCGCTCATTCCGACTATACGGGCGTTTGCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                  <  2:2349227/140‑1 (MQ=255)

                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                                  <  2:4177824/140‑1 (MQ=255)

                                                                       aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAACCAGATAAACCCGACAAAAACAAAAAATAATCTAGCAACTaaaat                                                              >  2:2936648/1‑138 (MQ=255)

                                                                       aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAACTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGCCAACAACAAAAAATAATCTAGCAAGTAAACt                                                              >  2:783628/1‑140 (MQ=255)

                                                                       aaGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACt                                                              >  2:125768/1‑140 (MQ=255)

                                                                        attCGATCATTCCGACTATACGGGCGTTTTATGATTTTTGTTTGAAGTTTTGGTAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                             <  2:2251727/138‑1 (MQ=255)

                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTATTTTTTGGAGATTTGAATGGCTTTTACGTTTTTTAAAAAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATATATCCAGTAAAATc                                                             >  1:2935395/1‑140 (MQ=255)

                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACCCGACAACAAAAAAAAAAAATATAGCAAGTAAACTc                                                             >  2:3246408/1‑140 (MQ=255)

                                                                         gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                            <  1:3121967/140‑1 (MQ=255)

                                                                          tCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGTAGTTTTGGGAGTTTTGAATTGGTTTTGCGTTTTTAAAACAAGAGGCAAGCCACATAAACCCGCCAACAAAAAAAAATAATATAGCAAATaaaaaaca                                                           >  1:2108289/1‑135 (MQ=255)

                                                                          tCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTGAAGTTTTTGGCGATTTTTATTGCTTTTGCGTTTTTAAAAAAAAAGGCAAACCACATAAAACCGACAAAAACAAAAAAAAAAAAAGCAAGTaaaataca                                                           >  1:542775/1‑135 (MQ=255)

                                                                            gATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGGTTTTACGTTTTTAAAAAAAGAGGCAACCCAGATAAACCCGACAACAACAAAAAATAAACTAGCAAGTAAACTAcatg                                                         >  2:591873/1‑140 (MQ=255)

                                                                             aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCt                                                                          >  2:1238118/1‑122 (MQ=255)

                                                                              tCATTCCGACTCTACGGGCGTTTTCGGATTTTTGGTTGAAGTTTGGGGATATTTGCATGGCTTGTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:3854271/140‑1 (MQ=255)

                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:2986808/140‑1 (MQ=255)

                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:1769580/140‑1 (MQ=255)

                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:840593/140‑1 (MQ=255)

                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:2239491/140‑1 (MQ=255)

                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:3186589/140‑1 (MQ=255)

                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:1139567/140‑1 (MQ=255)

                                                                              tCATTCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  2:2091300/140‑1 (MQ=255)

                                                                              tCAGTCCGACTATACGGGCGTTGTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAGGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                                       <  1:2666868/140‑1 (MQ=255)

                                                                                   ccGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGGATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATgggg                                                  <  1:1225241/140‑1 (MQ=255)

                                                                                          taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGTGCTAATTGAATGGGATTTCCGTTTTTAAAAAAAAAGGCAAGCCAAAAAAAACCGACAACAACAAAAAATAATCTAGCAAGTAAAATCCATGCAAGGGGTaaataa                                           >  2:2296394/1‑136 (MQ=255)

                                                                                          taCGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattaa                                           >  2:263712/1‑138 (MQ=255)

                                                                                                   tttCTGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGTCTTTTCCTTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAACTAAACTCCATGCATGGGGTAATTCATTCGTTGCa                                  <  1:1270010/140‑1 (MQ=255)

                                                                                                   tttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCa                                  <  1:784198/140‑1 (MQ=255)

                                                                                                   tttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCa                                  <  1:1547263/140‑1 (MQ=255)

                                                                                                        gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                             <  2:1509440/140‑1 (MQ=255)

                                                                                                              tgtttgAAGTTTTGGGAGATTTGAATTGCTTTTCCTTTTTTAAAACAAGAGTCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa                       <  2:3963105/140‑1 (MQ=255)

                                                                                                                    caGTTGTGGTAGATTTGAATTGCTTTTACTTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  2:2173227/139‑1 (MQ=255)

                                                                                                                    aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt                 <  2:4214723/140‑1 (MQ=255)

                                                                                                                      gTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTa               <  1:3248179/140‑1 (MQ=255)

                                                                                                                            tgagaTGTGACTGGCTTTACCGATTTTAAAACAATCTTCAAGCCAGATCAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTAGTTGCAAAATATAAAAATAGAGTTACTAAtg         <  2:2469782/139‑1 (MQ=255)

                                                                                                                              agaTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAtgtg       <  1:2612237/140‑1 (MQ=255)

                                                                                                                                aTTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACACCAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTCCTAATGTGcc     <  1:2062359/140‑1 (MQ=255)

                                                                                                                                 tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAATCCCGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCAAGAATTGGTTAATTCATTAGTTGCAAAATATAAAAATAGAGTTACTAAAGTGcag    >  2:2521719/1‑138 (MQ=255)

                                                                                                                                 tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg    <  2:2152657/140‑1 (MQ=255)

                                                                                                                                 tttGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCg    >  2:2264236/1‑140 (MQ=255)

                                                                                                                                   tGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTa  <  1:2469640/140‑1 (MQ=255)

AGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAATGTGCCGTA  >  NZ_CP034943/240075‑240345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`