Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,447,038 T→G 9.8% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,447,0380TG 9.8% 592.0 / 12.7 308N183T (AAC→ACC) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base T (113/151);  new base G (9/35);  total (122/186)
Fisher's exact test for biased strand distribution p-value = 4.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176
                                                                                                                                           |                                                                                                                                          
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGt                                                                                                                                            <  1:4028299/140‑1 (MQ=255)
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTATCACTGATAAACAAATATTAACCAAAAACGCGGt                                                                                                                                            >  2:3070331/1‑140 (MQ=255)
tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTCAAAATATTGCTAACATGGTTTTTCACGGTTTTCTCACTGATAAAAAAAGATTCACCAATAACGCGGt                                                                                                                                            >  2:1178788/1‑140 (MQ=255)
 ctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAATTCTCACATGGTTTTTCACGGTTTTATCACTTATAAAAAAAGAATAAACAATAACGCGGtt                                                                                                                                           >  2:2549290/1‑140 (MQ=255)
   cATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTCGCACAAGGTTTTTACCGGTTTTATCACCTATAAACACTTAGTCAACACTACCGCGGGTgg                                                                                                                                         >  1:3382240/1‑139 (MQ=255)
   cATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTTCTCACCTGGTTTTTCACGGTTTTCTCACTGGTAAACAATGTTTCACCAATGCCGCGGGTgg                                                                                                                                         >  1:3097043/1‑139 (MQ=255)
          aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTcc                                                                                                                                  <  2:2905974/140‑1 (MQ=255)
          aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATTAATTTTTTCAAAAAATTTCTGACATTGTTTTTGACGGGTTTGTGACTGATAAAAAAAGATTAACCCAAACCCCGGGTGGTTTTTcc                                                                                                                                  >  1:455604/1‑140 (MQ=255)
               gCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCCAAATATTGCTCACATGGTTTTTGACGGGTTTGTCACTGATAAACAATGATTCACCAAAACCCCGGTTGGTTTTTGCGtgtg                                                                                                                             >  1:307762/1‑140 (MQ=255)
                ccATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTAATTAATTTTTTTGAAAATAATTCTGCCAATGTTTTTCACGGTTTTATCACTTATAAACAAAGATTGACCAAAACCCCGGTTGCTTTTTACGTCAGc                                                                                                                            >  1:1006017/1‑140 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                                                                           <  2:2130487/140‑1 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                                                                           <  1:1472289/140‑1 (MQ=255)
                 cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                                                                           <  1:2859150/140‑1 (MQ=255)
                    ttttAATGGCGACCACACCGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  2:905636/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCACTACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  2:632311/140‑1 (MQ=255)
                    ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                                                                                        <  1:2300013/140‑1 (MQ=255)
                      ttAATGGCGACCACAACGGCTTGCGTCCGGACGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCAt                                                                                                                      <  2:1037369/140‑1 (MQ=255)
                            tcGACCCCAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  2:3347875/139‑1 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  1:2646761/140‑1 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTGTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  2:270020/140‑1 (MQ=255)
                            gCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                                                                                <  2:1229873/140‑1 (MQ=255)
                             cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAAAATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCagcagc                                                                                                               >  2:819835/1‑140 (MQ=255)
                              gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATGATTAAACAAAACCGCGGTTGCTTTTTCCGTCTGCAACCATCTgcagca                                                                                                              >  2:3614771/1‑140 (MQ=255)
                                   caACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                                                                                         <  1:2902423/140‑1 (MQ=255)
                                   caACGGCTTGCGTCCGGTCGTTTACATGCCTTTTTTGCAAAATATTGCTCACAGGGTTTGTCACGGTTTTCTCACTGATAAACAATGCTTCACCACTACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                                                                                         <  2:2673459/140‑1 (MQ=255)
                                           tGCGTCCGGTCGTTTACAATCATTTTTTGCAAAATATTGCTCAAAAGGTTTTTCACGGTTTTGTCCCTGATAAACAATGATTCACCAAAAACCCGGGTGCGTTTTACGTCTGCAAGCATCTGCCGCACATCACATTaccc                                                                                                 >  1:1964246/1‑139 (MQ=255)
                                           tGCGTCCGGTCGTGTACCTTCATTTTTTGCAAAATATTGCTCACATGGGTTTTCACGGTTTTCTCACAGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATATGCAGCACTTCACATTAccg                                                                                                 >  2:148014/1‑140 (MQ=255)
                                                  ggTCGTATACATTCAGTTTGCGCAAAAGAGTGCTCACCTGGATTTTCACGGTTTTCTCACTGAGACACAATGATTCACCACTACCGCGGTTGCTTTTTCCGTCGGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                                                                          <  2:2342128/140‑1 (MQ=255)
                                                   gTCTTTTACATTGATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACGAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCGGCAGCACTTCACATTCACGCCGAGTTa                                                                                         <  2:486838/140‑1 (MQ=255)
                                                   gTCGTTTACATTAATTTTTTGCAAAAAATTTCTCACATGGTTTTTCACGGTTTTATCAAAGATAAAAAATGATTAAACAATAACCCGGTTTCTTTTTCAGTCTTCAAACAACTCCCCCACTTCACATTACCGCCGACTTa                                                                                         >  2:3490130/1‑140 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:1126412/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  2:2641408/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:1298844/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:3920773/140‑1 (MQ=255)
                                                     cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                                                                                       <  1:736229/140‑1 (MQ=255)
                                                      gTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaaa                                                                                      <  1:1679350/140‑1 (MQ=255)
                                                              tcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTACCGCCGAGTTAAAATATGTAAt                                                                              >  2:4150737/1‑140 (MQ=255)
                                                              tcattTTTTGCAAAATATTGCTCACATGGTTTTTAAAGGTTTTATCACTGATAAAAAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCATCACTTCAAAATCCAGCCGAGTTAAAATATTTAAt                                                                              >  2:2073763/1‑140 (MQ=255)
                                                                    tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                                                                        <  2:2203917/140‑1 (MQ=255)
                                                                    tttGCAAAAGATTGCTCACATGGTTATTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg                                                                        <  2:1893129/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCCCCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:2089758/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:936123/140‑1 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  2:1946714/1‑140 (MQ=255)
                                                                      tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  2:1077956/1‑140 (MQ=255)
                                                                           aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt                                                                 <  2:3558514/140‑1 (MQ=255)
                                                                                tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGa                                                            <  2:3248497/140‑1 (MQ=255)
                                                                                tGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGa                                                            <  2:152346/140‑1 (MQ=255)
                                                                                   gcacaGGGTTGGTCACGGTTTTCTCACTGATAAACAATGATTCACCCATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCCCATTCCCGCCGAGTTTAAATATGTAATGGTCTGCGGATTTCAGGATAg                                                         <  1:870738/139‑1 (MQ=255)
                                                                                       aTGTTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCACTACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1859270/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAATCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3257875/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTACTGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:2222205/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:2224615/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:2782254/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:2582585/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:1712031/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:3552555/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:691058/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAGTTCAGGATAGACCt                                                     >  2:1669916/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:2540782/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGATTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTCATGGTCTGCGGATGTCAGGATAGACCt                                                     >  2:2435956/1‑140 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:3878242/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:1811759/140‑1 (MQ=255)
                                                                                       aTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  1:34931/140‑1 (MQ=255)
                                                                                         ggTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCa                                                   <  2:178355/140‑1 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     >  1:3102112/1‑135 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCt                                                     <  2:3102112/135‑1 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGc                                                >  2:2251326/1‑140 (MQ=255)
                                                                                            ttttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGc                                                >  2:574928/1‑140 (MQ=255)
                                                                                             tttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      <  1:1548643/117‑1 (MQ=255)
                                                                                             tttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg                                                                      >  2:1548643/1‑117 (MQ=255)
                                                                                              ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg                                              <  2:455604/140‑1 (MQ=255)
                                                                                              ttCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTg                                              <  2:1622141/140‑1 (MQ=255)
                                                                                              ttCACGGTTTTCTCACTGATAAACAATGATTCAACAATACCGCGGTTGCTTTTTCCGACTGCAAGCATCTGCAGCACTTCACCTTCCCGCCCAGTTAAAATATGTAATGGTGTGCGGATTTCATGATAGACATCATGCTg                                              <  2:13946/140‑1 (MQ=255)
                                                                                                 aCGGTTTTCTCACTTATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCAGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGATTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAgg                                           <  2:1904252/140‑1 (MQ=255)
                                                                                                         ctcACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAg                                   <  2:2055437/140‑1 (MQ=255)
                                                                                                          tcACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGa                                  <  2:2979790/140‑1 (MQ=255)
                                                                                                           cACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGGCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGaa                                 <  2:1358278/140‑1 (MQ=255)
                                                                                                                   aaCAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATTTAATGGTCTGCGGATTTCAGGATAGACCTCATGATGAGGGTGTGAATAAACTCCGCt                         >  1:3644159/1‑140 (MQ=255)
                                                                                                                      aaTGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                                                                         <  2:2585888/73‑1 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGa                                             >  1:2196639/1‑115 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAggg                                          <  2:4015526/118‑1 (MQ=255)
                                                                                                                        tGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCttgttg                    >  2:3854359/1‑140 (MQ=255)
                                                                                                                          aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  1:1206928/1‑140 (MQ=255)
                                                                                                                          aTTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCa                  >  2:3661543/1‑140 (MQ=255)
                                                                                                                              aCCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggc              >  1:663670/1‑140 (MQ=255)
                                                                                                                              aCCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggc              >  1:201405/1‑140 (MQ=255)
                                                                                                                                cAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAggcgg            >  1:1762695/1‑140 (MQ=255)
                                                                                                                                   tACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGcggcgg         >  2:2844668/1‑140 (MQ=255)
                                                                                                                                    aCCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGa        <  2:3829177/140‑1 (MQ=255)
                                                                                                                                      cgcgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAAt      >  1:2995509/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGTAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  1:2444594/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAGTATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  1:812541/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAt                                                           <  1:1156045/85‑1 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  1:4156983/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  1:553897/1‑140 (MQ=255)
                                                                                                                                        cgGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTc    >  2:1328978/1‑140 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGtgtg                                      >  2:4117320/1‑104 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGtgtg                                      >  2:3288288/1‑104 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGtgtg                                      >  2:2787729/1‑104 (MQ=255)
                                                                                                                                          gTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGt  >  1:3843158/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGATAGACCTCATGCTGAGGGTGTGCAGAAACTCCGCTTGTTGCAAGGCGGCGGAATTCGT  >  NZ_CP034943/3446899‑3447176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: