Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,851,237 G→T 10.4% I46I (ATC→ATA EO946_RS19820 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,851,2370GT10.4% 637.7 / 11.1 283I46I (ATC→ATAEO946_RS19820hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (178/59);  new base T (3/40);  total (181/102)
Fisher's exact test for biased strand distribution p-value = 8.82e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376
                                                                                                                                           |                                                                                                                                           
cAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTTTATCATg                                                                                                                                             >  1:312425/1‑140 (MQ=255)
   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                          <  1:1259187/140‑1 (MQ=255)
     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGTACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:2203511/1‑140 (MQ=255)
     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:2675657/1‑140 (MQ=255)
     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:655171/1‑140 (MQ=255)
     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:1742677/1‑140 (MQ=255)
     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                        >  2:3140361/1‑140 (MQ=255)
        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     <  1:375656/140‑1 (MQ=255)
        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     >  2:774296/1‑140 (MQ=255)
        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     <  2:2842680/140‑1 (MQ=255)
        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     <  2:1357239/140‑1 (MQ=255)
        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                     <  1:2526661/140‑1 (MQ=255)
        cAATATGCTATCTGTAATATTGATTTTTAATGGAATTCTCTTTTCTCTAGTTTTATCACCTTGTAAGCATAACCTTCTATGTACTCGGACAGGTCTTCTATTAATGAACCAGGCGTTTTTACCTGTATCATGATTCGATc                                                                                                                                     <  2:1805092/140‑1 (MQ=255)
         aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                    <  1:339532/140‑1 (MQ=255)
               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACATGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCa                                                                                                                              >  2:2579630/1‑140 (MQ=255)
               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                    <  1:949248/134‑1 (MQ=255)
               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  2:1062143/1‑140 (MQ=255)
               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  1:2256670/1‑140 (MQ=255)
               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                              >  2:298551/1‑140 (MQ=255)
               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATgaa                                                                                                                              >  1:1299949/1‑138 (MQ=255)
                   ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                          <  2:3015697/140‑1 (MQ=255)
                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                         >  1:849664/1‑140 (MQ=255)
                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                         >  2:838390/1‑140 (MQ=255)
                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGAAAGccc                                                                                                                         >  2:3567325/1‑140 (MQ=255)
                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTccc                                                                                                                    >  1:915190/1‑140 (MQ=255)
                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGAAAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTcca                                                                                                                    >  2:4181632/1‑139 (MQ=255)
                             gATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                          >  1:262519/1‑114 (MQ=255)
                              aTTGGAAATGGCATTCTCTTTTCGCTAGTTTTATCACCTGGTACGCCTAACCTGCACTGTACTCGGACAGGGCTTCGATGATTGAACCCGTCGTTATTCCGTGGAGCGGGATTCGCTCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  2:3325412/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:1099718/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCaaaaa                                                                                                               >  1:1341560/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  2:105365/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:997507/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:3172013/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:1817169/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:1284436/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:2934414/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAATCCAGTACCaaaaa                                                                                                               >  2:1854355/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                               >  1:1197330/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACATTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATAATGATTCGAAAGTATTCAAGCCCTTCACaaaaa                                                                                                               >  1:3986682/1‑140 (MQ=255)
                              aTTGGAAATGGAATTCTCTTGTCGCTAGTTTTATCACCTTGTAAGCATAAACTTCAAAGTAATCGGACAGGTCTTCTATAAATGAACCAGTCGATAATACCAGTATCATGAATAGATATTAATCAAGCCCTTACCacaaa                                                                                                               >  1:3576149/1‑140 (MQ=255)
                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                             >  2:3251679/1‑140 (MQ=255)
                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAACg                                                                                                             >  1:1936631/1‑140 (MQ=255)
                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                             >  1:141224/1‑140 (MQ=255)
                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCaaaaaag                                                                                                             >  1:1430414/1‑138 (MQ=255)
                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACAGTATCATTATTAGATAGTATGCAAGCCCGTCCCCAAAACg                                                                                                             >  1:3236646/1‑140 (MQ=255)
                                  gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGTTCGTATGCAAGCCCTTGCCAAAAACGAt                                                                                                           >  1:2551003/1‑140 (MQ=255)
                                        gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACg                                                                                                     >  2:2112717/1‑140 (MQ=255)
                                                ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGct                                                                                             >  1:2119253/1‑140 (MQ=255)
                                                ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCATCCCAAAAACCATAAAACGACTCTGct                                                                                             >  1:572306/1‑140 (MQ=255)
                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTAGTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                         >  2:2237533/1‑140 (MQ=255)
                                                                     tttAAGCATAACCTTAAATTTACTCGGAATGTTCTTATATAAATCAACCAGTCTTTTTTACCTTTATCATTTTTCGATCTTCGGAACGCCCTGCCCAAACCCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                        <  2:1121209/138‑1 (MQ=255)
                                                                         agaaTAACCTTCAAAGTACTCGCACAGTTCTTCTATAAATCAACCCGTCGTTATTACCTGTAACATTATTCGATCGTTTGCAAGCCCTCCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                                    <  2:1284436/137‑1 (MQ=255)
                                                                         aGCATAACCTTAAATTTACTCGCACAGTTTTTATATAAATCAACCTGTCTTTATTACGTTTATCATTTTTCTATGTTTTGCAAGCCCTCCCCAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                                    <  2:3721851/140‑1 (MQ=255)
                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTtg                                                                >  1:3879418/1‑140 (MQ=255)
                                                                                cctaaaaaTTACTCCCAAAGGTCTTATATAAAGTACCGATGCGTTTTTACCTGTATCTTTATTCGATCTTATGCACGCCCTTCAAAAACACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  1:2429998/135‑1 (MQ=255)
                                                                                cctaaaaaGAACGCGCACTGGTCTTATATAAATCAACCCGTCTTTATTACCTTTATAATGATTCGATCTTTTCCACGCCCTACCAAAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  1:1906969/135‑1 (MQ=255)
                                                                                ccTTCAAATTACCCGGACAGGTCTTATAAAAATGACCCAGTCGTTTTTACCGGGATCCTGATACCATGGTATGCACGCCCGGCCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2369231/140‑1 (MQ=255)
                                                                                ccTTCAAAGTACTCGCACAGTTCTTATATAAATTAACAAGTCTTTATTAACTTTATCATTATTCGATCTTATGCAAGCCCTTCCCAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2067720/140‑1 (MQ=255)
                                                                                ccTACAAAGTACTCGGACTGTTCTTATATAAATCACCCAGTCTTTATTACCTGTATCATTATGCGATCGTATCCAAGCCCTACCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  1:3140361/140‑1 (MQ=255)
                                                                                cattCAAATTACTCGCACAGGTCTTATATAAATTAACCAGTGTTTATTACGTGTATATTTATTCGCTCGTATGAAACCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:4263701/138‑1 (MQ=255)
                                                                                catccAATGTACTCGCACTGGTTTTCTATAAATTAACCAGTGTTTTTTACATTTATCATGATTCGATCGTTTGCACGCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2517111/136‑1 (MQ=255)
                                                                                 cTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCCAGACATAACAACTtgatga                                                            >  2:541099/1‑140 (MQ=255)
                                                                                     aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCCTGATTCGCGCGTATGCCAGCCCGTCCCAAAAACGATAACACGACTCTGCTTGTTCCACTTCGCAAGACAGACACACGTGATGAGGCt                                                        >  1:622845/1‑140 (MQ=255)
                                                                                     aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCt                                                        >  1:1591163/1‑140 (MQ=255)
                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTTTCATGATTCGTTCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACACCTTGTTGAGGCTTTagag                                                  >  1:3336452/1‑140 (MQ=255)
                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  1:4150338/1‑140 (MQ=255)
                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  1:2867646/1‑140 (MQ=255)
                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  2:462494/1‑140 (MQ=255)
                                                                                            tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCGGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGc                                                 >  2:1984475/1‑140 (MQ=255)
                                                                                              ggACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAAGATTAGATCGTATGAAAGCCCGTCCCAAAAACGATAAAACCACACAGATTCTTAAAGTTCTCAAGACATAAAAACTTTTTGATGCTTAAGAGCGa                                               >  1:2321103/1‑140 (MQ=255)
                                                                                               cactgctcttatATAAATCAACCTGTGTTTATCACTTGTATCATTTTACCACCTTAGGAAAGCCCAACCCAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              <  1:2732987/134‑1 (MQ=255)
                                                                                               caccggTATTCAATAAATCAACCTGTCTTTATTACCTTTAACTTGTTTCGATCGTCTGCACGCCGTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              <  1:105365/136‑1 (MQ=255)
                                                                                                         ttaaatttGAAACAGTTGTTATTAATTTTATATTGTTTTGAGCGTATGAATGCACTTCCCAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa                                    <  1:731050/133‑1 (MQ=255)
                                                                                                         ctATAAATGAACCAGTTGTTATTACCTTTATAATGTTTCTATCTTATCAACGCCCTACCAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa                                    <  1:3710280/140‑1 (MQ=255)
                                                                                                         ataaaaaTCAACCTGTGTTTTTTACTTTTATGATTATTCGATGGTTGGAACGCCCTCCCAAACCCCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa                                    <  1:1726110/136‑1 (MQ=255)
                                                                                                          tataAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                   >  1:3027135/1‑140 (MQ=255)
                                                                                                          tataAATCAACCAGTCTTTATTACATTTATCTTTATTCGATCGGATGCAAGCCCTCCCCAACAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                   <  2:4156432/140‑1 (MQ=255)
                                                                                                           ataAATCAACCATTCGTTTTTACTTTTAACATTTTTCGATTGTATGAAAGCCCTACCCAAACCCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAtt                                  <  2:1299949/140‑1 (MQ=255)
                                                                                                             aaaTGAACCAGTCTTTATTACCTTTATCTTGATTCGATCGTCTGAAAGCCCGTCCCAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  2:495772/140‑1 (MQ=255)
                                                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                               >  2:2344528/1‑140 (MQ=255)
                                                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                               >  1:447328/1‑140 (MQ=255)
                                                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGGATGCAAGCCCTTGCCAAAAACGATAAAACGAAACTGCTTATTCAAGTTAGCAATTCATTACAAAATGCTGAGGCTTAAGATCGATCATTTATAAAATGACg                              >  1:2697108/1‑140 (MQ=255)
                                                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTAGCAAGACATAACAACTTGATGAGGCTTTAGAGCGATAATGTCTAAATTGCCg                              >  1:1689743/1‑140 (MQ=255)
                                                                                                               aTGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACACTGCTTATTCAAGTTAGCAAAACATAACAACGTGATGAGGCTTAAAAGCGATAAAGTCTAAAATTCCg                              >  1:4040753/1‑140 (MQ=255)
                                                                                                                 caaCCCGTCTTTATAACCTTTACCTTTTTTCGATCTTACCCACGCCCTACCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                            <  1:516862/139‑1 (MQ=255)
                                                                                                                 caaCCAGTCTTTATCACCTTTATCATTATGCGATCGTCTGCACGCCCTTCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                            <  2:1705780/139‑1 (MQ=255)
                                                                                                                  aaCCAGTCTTTATTACCTGTATAATTATTCGATCGTAGGCAACCCCTCCCAAAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:3879418/140‑1 (MQ=255)
                                                                                                                  aaCCAGTCTTTATTACCTGTATAATGATTCTATCGTATGCAACCCCTTCCAAAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:915190/140‑1 (MQ=255)
                                                                                                                  aaCCAGTCGTTTTTCCCTGTATCATTTTTCGATCGTATGAAAGCCCTACCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:1416527/140‑1 (MQ=255)
                                                                                                                  aaCCAGTCGTTATTACCTGTAGAATGATTCTATCTTATCCAACCCCTGCCAAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:1198520/140‑1 (MQ=255)
                                                                                                                   aCCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCCAGTTCGCAACACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCCATAc                          >  1:2909232/1‑140 (MQ=255)
                                                                                                                       gTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCt                      >  2:3491587/1‑140 (MQ=255)
                                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa           >  2:2760156/1‑140 (MQ=255)
                                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa           >  2:1461573/1‑140 (MQ=255)
                                                                                                                                    gTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt         >  2:3996302/1‑140 (MQ=255)
                                                                                                                                    gTATAATCATTCGATCTTAGGCAACCCCTGCCAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGCCATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt         <  2:424361/140‑1 (MQ=255)
                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  2:2089294/1‑140 (MQ=255)
                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  2:166998/1‑140 (MQ=255)
                                                                                                                                     aaTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  2:3401370/2‑140 (MQ=255)
                                                                                                                                      aTCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc       >  2:2754844/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: