Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,976,114 G→T 6.4% A31E (GCA→GAA)  rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,976,1140GT 6.4% 753.8 / 2.7 294A31E (GCA→GAA) rlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base G (113/148);  new base T (30/3);  total (143/151)
Fisher's exact test for biased strand distribution p-value = 1.11e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

CGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAA  >  NZ_CP034943/3975977‑3976253
                                                                                                                                         |                                                                                                                                           
cGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCg                                                                                                                                           <  1:3035584/140‑1 (MQ=255)
cGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCg                                                                                                                                           <  1:554562/140‑1 (MQ=255)
cGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCg                                                                                                                                           <  1:3190684/140‑1 (MQ=255)
cGAGGGCGATGACGTGGGCGTAGGGGCTGATTTTGGATAGGATGCGGTCGCGTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTGGTCCGGGAGTTCGATGATGTCGATTTTTGCg                                                                                                                                           >  1:196856/1‑140 (MQ=255)
    ggCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTGCGGGGCTTTTTCGTGCGGGAGTTCGTTGTTGTCGATTTTTGCGTggg                                                                                                                                       >  1:3860366/1‑140 (MQ=255)
    ggCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAgg                                                                                                                                       >  1:3119672/1‑140 (MQ=255)
    ggCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCGTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGCGTTCGATGATGTCGATTTTTTCGTAgg                                                                                                                                       >  1:3530641/1‑140 (MQ=255)
    ggCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCGTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTGGATGATGTCGATTTTTGCGTAgg                                                                                                                                       >  1:1929984/1‑140 (MQ=255)
       gATGACGTGGGCGTCGTGGATGATTTTGGATAGGATGCGGTAGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCg                                                                                                                                    <  1:1060529/140‑1 (MQ=255)
        aTGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGa                                                                                                                                   <  2:2227175/140‑1 (MQ=255)
         tGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGaa                                                                                                                                  >  1:624621/1‑140 (MQ=255)
             gTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTc                                                                                                                              <  1:1537233/140‑1 (MQ=255)
              tGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGGAGGCCGAAAGTct                                                                                                                             >  2:4001117/1‑139 (MQ=255)
              tGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCCCTTTTTAGTCCGGGAGTTCGATGATGTCGAATTTTGCGTATGCCGAAACTCg                                                                                                                             >  1:2829040/1‑140 (MQ=255)
               gggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGGCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGt                                                                                                                            >  1:4216010/1‑140 (MQ=255)
               gggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTGCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCGTTTTGGTCCGGGAGTTCGATGTTGTCGATTTTTGGGTATGCCGAAAGTGGt                                                                                                                            >  1:1702931/1‑140 (MQ=255)
                ggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCGTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGtt                                                                                                                           >  2:1692409/1‑140 (MQ=255)
                ggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTAGTGCGGGAGTTAGATGATGTAGATTTTTGCGTAGGCCGAAAGTCGtt                                                                                                                           >  1:1830409/1‑140 (MQ=255)
                ggCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTGCTTTGTCTTTTATGATTGTCATGTCCTGGTCGCTGAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGtt                                                                                                                           >  2:3146369/1‑140 (MQ=255)
                 gCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGttt                                                                                                                          >  2:695815/1‑140 (MQ=255)
                  cGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTc                                                                                                                         <  1:857011/140‑1 (MQ=255)
                  cGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTc                                                                                                                         <  2:3540907/140‑1 (MQ=255)
                     cGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTGTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCgtg                                                                                                                      >  2:2851220/1‑140 (MQ=255)
                      ggggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCgtgt                                                                                                                     >  1:3722239/1‑140 (MQ=255)
                       gggCTGATTTTGGATGGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGCAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:264367/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:2518001/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:3996494/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:3643886/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  2:190115/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  2:2124284/140‑1 (MQ=255)
                       gggCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTa                                                                                                                    <  1:1268114/140‑1 (MQ=255)
                            gATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTAttctt                                                                                                               <  1:3436751/140‑1 (MQ=255)
                             aTTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTAttctta                                                                                                              >  2:1274622/1‑139 (MQ=255)
                               tttGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTATCCGGCGCTTTTTCGTCCCGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGACGTTTCGTGTATTCTTaaa                                                                                                            >  2:4120398/1‑140 (MQ=255)
                                       ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgc                                                                                                    >  1:1033798/1‑140 (MQ=255)
                                        gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTTGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGACGTTTAGTGTATTCTTAAATGCcttgct                                                                                                   >  1:2240354/1‑140 (MQ=255)
                                        gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATGCctggct                                                                                                   >  2:364992/1‑140 (MQ=255)
                                        gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCATAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTAGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgct                                                                                                   >  1:697583/1‑140 (MQ=255)
                                          tGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgcttg                                                                                                 <  1:1645186/140‑1 (MQ=255)
                                             ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAgg                                                                                              >  2:2738979/1‑140 (MQ=255)
                                              gTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTATGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATTATTTCGATTTTTTCGTAGGCCGAAAGTCGTTTGGTGTATTATTAAATTCCTGTGTTGAGGt                                                                                             >  1:1598171/1‑140 (MQ=255)
                                                  ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCGTTTTCGTCCGGGAGTTAGATGATTTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATTCCTTGCTTGAGGTAttt                                                                                         >  1:961281/1‑140 (MQ=255)
                                                  ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCCGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTAGGTTCGTGTATTATTAAAAGACTTGGTTGAGGTAatt                                                                                         >  1:1343202/1‑140 (MQ=255)
                                                      cttTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTAGTTTCGTGTATTATTAAATTCCGTGCTTTAGGTAtttttgt                                                                                     >  1:3812393/1‑138 (MQ=255)
                                                        ttGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCttt                                                                                   <  1:2194639/140‑1 (MQ=255)
                                                             ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAttt                                                                              >  2:2308771/1‑140 (MQ=255)
                                                             ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTAGTGTATTATTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGtt                                                                              >  2:2630004/1‑140 (MQ=255)
                                                             ttttATGATTTTCATGTCCTGGTCGCGTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGTTGATGTCGAATTTTGCGTATTCCGAAAGTCGTTTCGTGTATTCTTCAAAGACTTGCATGAGGTATTATTATTTTAGtt                                                                              >  2:925315/1‑140 (MQ=255)
                                                                tATGATTTTCATGTCCTGGTCGGTTAGCTTTTCAGGCGCTTTTTCGTCCGGGAGTTCTATGATTTCGATTTTTTCGTAGGCCGAAATTCGTTTAGTTTATTATTGAATGACTGTCTGTAGGTATTTTTATGTTaatgtta                                                                           >  1:467405/1‑134 (MQ=255)
                                                                tATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTACGGGAGTTAGATGATGTCGATTTTTGCGTAGGCCGAAAGTAGTTTCGTGTATTCTTGAAATCCGTGCGTGACGTATTTTTCTTTTATtttta                                                                           >  1:2333462/1‑139 (MQ=255)
                                                                tATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATTCCTTGCTTTATGTATTTTTATTTTATtttta                                                                           >  1:3928175/1‑139 (MQ=255)
                                                                   gATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGa                                                                        <  2:871982/140‑1 (MQ=255)
                                                                   gATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGa                                                                        <  2:3887929/140‑1 (MQ=255)
                                                                    aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                       <  2:2719093/140‑1 (MQ=255)
                                                                    aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                       <  1:1916648/140‑1 (MQ=255)
                                                                    aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                       <  2:3631571/140‑1 (MQ=255)
                                                                     ttttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAtt                                                                      >  2:4138327/1‑140 (MQ=255)
                                                                     ttttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTATTTTAGTTTTACGAtt                                                                      >  2:2899067/1‑140 (MQ=255)
                                                                         cATGTCCTGGTCGCTGAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTca                                                                  <  1:146810/140‑1 (MQ=255)
                                                                          aTGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGAGTAGGCCGAAAGTCTTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTcac                                                                 <  2:4203524/140‑1 (MQ=255)
                                                                           tGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTcaca                                                                <  2:1963579/140‑1 (MQ=255)
                                                                            gTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACaa                                                               <  2:3420336/140‑1 (MQ=255)
                                                                               cTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAGGTATTTTTGTTTTAGTTTTCCGATTTTCAAAatat                                                            >  2:1514400/1‑140 (MQ=255)
                                                                                 ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                          <  1:1499616/140‑1 (MQ=255)
                                                                                 ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                          <  2:3371640/140‑1 (MQ=255)
                                                                                 ggTCGCTTAGGTTGTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                          <  1:3380277/140‑1 (MQ=255)
                                                                                  gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTTAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGa                                                         >  2:3806164/1‑140 (MQ=255)
                                                                                  gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTAAATTCCTTGCTTGAGGTATTTTTATTTTATTTTTACGATTTTCACAATATTGa                                                         >  2:1776536/1‑140 (MQ=255)
                                                                                  gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTACGATTGTCAAAAAATTGa                                                         >  2:2646378/1‑140 (MQ=255)
                                                                                       ttcggTTTTCAGGCGCTTTTTCGTACGGGGGTTCGATGATGGCGATTGTTTCGTAGGCCGAAAGTGGTTTCGTGTTTTCTTGAATGCCGTGCGTGAGTTAATTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTc                                                    >  2:1111067/4‑140 (MQ=255)
                                                                                        tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                   <  1:280445/140‑1 (MQ=255)
                                                                                               tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                            >  2:1815908/1‑140 (MQ=255)
                                                                                               tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                            >  2:1473963/1‑140 (MQ=255)
                                                                                                ccGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCAt                                           >  2:3304451/1‑140 (MQ=255)
                                                                                                 cGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATc                                          >  2:591089/1‑140 (MQ=255)
                                                                                                     gcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGGATTCTTCAATGCCTGGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAAAATTGATATTCACAGGTCATCCCCa                                      >  1:1141704/1‑140 (MQ=255)
                                                                                                              tCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTaaaaa                             <  2:1941303/140‑1 (MQ=255)
                                                                                                                  ggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                         <  2:3267042/140‑1 (MQ=255)
                                                                                                                   gAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGt                        <  1:2726271/140‑1 (MQ=255)
                                                                                                                   gAGTTCGATGATGTCGATTTTTGAGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTTCTTGAGGTATTTTTCTTTTAGTTTTCCGATTTTCACAATATTGCTATTCACAGGTCATCCCCACTTTAAAAACAAGt                        <  2:543071/140‑1 (MQ=255)
                                                                                                                    aGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGtt                       <  2:1488519/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:3081877/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:1563660/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:1997747/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:406468/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:3489984/140‑1 (MQ=255)
                                                                                                                     gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                      <  2:2890532/140‑1 (MQ=255)
                                                                                                                     gTTAGATGATGTCTTTTTTTGCGTAGTCCTAAAGTCGTTTCGTTTATTCTTCAATGCCTTTATTGAGGTATTTTTCTTTTATTTTTCAGATTGTCTGAATATTGATATTCACATTTAATCCCCACTTTAAAAACAAGTTa                      <  2:2419521/140‑1 (MQ=255)
                                                                                                                      ttCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAt                     <  1:1984325/140‑1 (MQ=255)
                                                                                                                       tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTCGTTTACCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                    <  2:2142314/140‑1 (MQ=255)
                                                                                                                       tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                    <  2:1033798/140‑1 (MQ=255)
                                                                                                                       tCGATGATGTAGATTTGTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                    <  2:3512803/140‑1 (MQ=255)
                                                                                                                        cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                   >  1:411822/1‑140 (MQ=255)
                                                                                                                         gatgatGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACt                                    <  1:2960681/122‑1 (MQ=255)
                                                                                                                         gatgatGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACt                                    >  2:2960681/1‑122 (MQ=255)
                                                                                                                               ggcgctttgtGCGTCGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            <  2:2992183/135‑1 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            <  1:1877731/140‑1 (MQ=255)
                                                                                                                               gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg            <  1:213405/140‑1 (MQ=255)
                                                                                                                                    tttttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTc       <  1:2646378/140‑1 (MQ=255)
                                                                                                                                    tttttGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTc       <  1:1776536/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CGAGGGCGATGACGTGGGCGTCGGGGCTGATTTTGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAA  >  NZ_CP034943/3975977‑3976253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: