Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,976,149 G→T 8.1% G19G (GGC→GGA rlmH ← 23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,976,1490GT 8.1% 715.7 / 14.1 279G19G (GGC→GGArlmH23S rRNA (pseudouridine(1915)‑N(3))‑methyltransferase RlmH
Reads supporting (aligned to +/- strand):  ref base G (93/153);  new base T (30/3);  total (123/156)
Fisher's exact test for biased strand distribution p-value = 4.07e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

TGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976010‑3976287
                                                                                                                                           |                                                                                                                                          
      ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgc                                                                                                                                      >  2:388984/1‑140 (MQ=255)
      ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTCCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgg                                                                                                                                      >  2:401909/1‑139 (MQ=255)
      ggATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCGTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgc                                                                                                                                      >  1:1033798/1‑140 (MQ=255)
       gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTCAATGCctggct                                                                                                                                     >  2:364992/1‑140 (MQ=255)
       gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTATTAAAATCctttct                                                                                                                                     >  1:2645688/1‑140 (MQ=255)
       gATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCATGGTCGGTTAGGTTTTCCGGCGCTTTTTCGTAAGGGAGTTCGATGATGTCGATTTTTGGGTAGGCCGAAAGTCGTTTCGTGTTTTCTTCAATGCcttgct                                                                                                                                     >  2:3101286/1‑140 (MQ=255)
         tGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCcttgcttg                                                                                                                                   <  1:1645186/140‑1 (MQ=255)
            ggTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAgg                                                                                                                                >  2:2738979/1‑140 (MQ=255)
             gTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTATGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGATTATTTCGATTTTTTCGTAGGCCGAAAGTCGTTTGGTGTATTATTAAATTCCTGTGTTGAGGt                                                                                                                               >  1:1598171/1‑140 (MQ=255)
             gTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGt                                                                                                                               >  2:196156/1‑140 (MQ=255)
             gTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGt                                                                                                                               >  2:2237621/1‑140 (MQ=255)
                gCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAtt                                                                                                                            <  2:3192559/140‑1 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTATGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATTATGTCGATTTTTGCGTATGCCGAAAGTAGTTTAGTGTATTATTAAAATCCGTGCTTGAGGTAttt                                                                                                                           >  1:886912/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAttt                                                                                                                           >  2:1499616/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGGCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCTTGCTTGAGGTAttt                                                                                                                           >  1:2814530/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTAGTCCGGGAGTTCGATGATGTCGTTTTTTGCGTAGGGCGAAAGTCGTTTAGTGTATTATTAAATTCCTTGCGTGAGGTAttt                                                                                                                           >  1:1815172/1‑140 (MQ=255)
                 ccTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCCCGTTTTATTACGGGACTTAGAAGATGTCGATTTTTTGGTAGGCCGAAATTAGTTTGGTGTAATATTAAATTACTTGCTTGTGGTAatt                                                                                                                           >  1:2459766/1‑140 (MQ=255)
                            ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTATTTTAGtt                                                                                                                >  2:4261391/1‑140 (MQ=255)
                            ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCCAAAGTCGTTTAGTGTATTCTTCAATGCCTTGCTTGAGGTAATTTTCTTTTAGtt                                                                                                                >  2:2410681/1‑140 (MQ=255)
                            ttttATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTAGATGATGTCGATTTTTGCGTAGGCCGAAACTCGTTTCGTGTATTATTCAATGCCTTGCTTGAGTTATTTTTCTTTTAGtt                                                                                                                >  2:3538006/1‑140 (MQ=255)
                            ttttATGATTTTCATGTCCTGGTCGCGTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTCGTTGATGTCGAATTTTGCGTATTCCGAAAGTCGTTTCGTGTATTCTTCAAAGACTTGCATGAGGTATTATTATTTTAGtt                                                                                                                >  2:925315/1‑140 (MQ=255)
                               tATGATTTTCATGTCCTGGTCGGTTAGCTTTTCAGGCGCTTTTTCGTCCGGGAGTTCTATGATTTCGATTTTTTCGTAGGCCGAAATTCGTTTAGTTTATTATTGAATGACTGTCTGTAGGTATTTTTATGTTaatgtta                                                                                                             >  1:467405/1‑134 (MQ=255)
                               tATGATTTTCATGTCCTGGTCGCTTAGGTTTTACGGCGCTTTTTCGTCCGGGAGTTAGATGATGTCGATTTTTGCGTATGCCGAAATTCGTTTTGTGTATTATTAAATTGCTTGCTTGATGTATTTTTATTTTAGtttta                                                                                                             >  1:3731680/1‑139 (MQ=255)
                                  gATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGa                                                                                                          <  2:871982/140‑1 (MQ=255)
                                   aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  2:1789554/140‑1 (MQ=255)
                                   aTTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGAt                                                                                                         <  2:2719093/140‑1 (MQ=255)
                                    ttttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTATGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCGTGCTTGAGGTATTTTTCTTTTATTTTTCCGAtt                                                                                                        >  2:4168009/1‑140 (MQ=255)
                                      ttCATGTCCTGGTCTCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATTCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                      <  2:1929984/140‑1 (MQ=255)
                                      ttCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGATTTCGATTATGTCGATTTTTGCGTAGGCCGAAAGTAGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGt                                                                                                      <  1:2196247/140‑1 (MQ=255)
                                          tGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTcaca                                                                                                  <  2:393453/140‑1 (MQ=255)
                                           gTCCTGTTCGCTTAGGTTTTCCGGGGGTTTTTCGTCCGGGAGTTCTATGATGTCGATTTTTGCGTCGGCCCAAATTCGTTTCGTGAATACTTAACTGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACaa                                                                                                 <  2:1841789/140‑1 (MQ=255)
                                                ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  1:65399/140‑1 (MQ=255)
                                                ggTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTg                                                                                            <  1:1499616/140‑1 (MQ=255)
                                                 gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGGTTGAGGTATTTTTCTTTTAGTTTTCCGATTTTCACAATATTGa                                                                                           >  2:891662/1‑140 (MQ=255)
                                                 gTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTTAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGa                                                                                           >  2:3806164/1‑140 (MQ=255)
                                                 gTCGCTTAGGTTTTACGGCGCTTTTTAGTGCGGGAGTTCGATGATGTCGATTTTTTCGTAGGCCCAAAGTCGTTTGGTGTATTCTTAAAATACTTTGTTGAGGTATTTTTGTTTTATTTTTACGATTGTAACAAAAttta                                                                                           >  1:190242/1‑138 (MQ=255)
                                                   cGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGata                                                                                         <  1:792180/140‑1 (MQ=255)
                                                      ttAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTGCGGGTATTATTCAATGCCGTGCTTGAGGGATTTTTCTTTTAGTTTTACGATTGTGACAATATTGAGAtta                                                                                      >  2:3406031/1‑139 (MQ=255)
                                                       tAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTca                                                                                     <  1:1507154/140‑1 (MQ=255)
                                                            tttCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGt                                                                                <  1:3430350/140‑1 (MQ=255)
                                                             ttCCGGCGCTTTTTACTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAAATCCTTGCTTGATGTATTTTTCTTTTACTTTTCCGATAGTCACAATATTGATATTCACATGTc                                                                               >  2:4120638/1‑140 (MQ=255)
                                                              tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  2:3454693/1‑140 (MQ=255)
                                                              tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGGTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAAGATTGATATTCACAGGTCa                                                                              >  1:1338925/1‑140 (MQ=255)
                                                              tCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGGCGTTTCGGGTATTCTTCAATGCCTTGCGTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCa                                                                              >  2:1520845/1‑140 (MQ=255)
                                                               ccGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCAt                                                                             >  2:3304451/1‑140 (MQ=255)
                                                                cGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATc                                                                            >  2:2900121/1‑140 (MQ=255)
                                                                  gcgcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATccc                                                                          <  1:1149079/140‑1 (MQ=255)
                                                                  gcgcTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTGGCTTGAGGTATTTTTCTTTTAGTTTTCCGAGTGTCACAATATTGATATTCACAGGTCATccc                                                                          >  2:556111/1‑140 (MQ=255)
                                                                      tttttCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTGTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACt                                                                      <  2:1183794/140‑1 (MQ=255)
                                                                                 ggAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAg                                                           <  2:1482618/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCTCTTTAAAAACAAGTTa                                                        <  1:1706627/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:143620/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:653306/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:1563660/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:3506889/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  1:1858356/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:4116389/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:1997747/140‑1 (MQ=255)
                                                                                    gTTCGATGATGTCGATTTTTGAGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTa                                                        <  2:2968878/140‑1 (MQ=255)
                                                                                     ttCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAt                                                       <  1:1984325/140‑1 (MQ=255)
                                                                                     gtCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAt                                                       <  1:209872/139‑1 (MQ=255)
                                                                                      tagatgatGTCGATTTTTGCGTAGGCAGAAAGTCGTTTCGTGTATTCGTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCAGATTGTCACATTATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:2037732/138‑1 (MQ=255)
                                                                                      tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:2633751/140‑1 (MQ=255)
                                                                                      tCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:1033798/140‑1 (MQ=255)
                                                                                      tCGAGGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTAtt                                                      <  2:3285803/140‑1 (MQ=255)
                                                                                       cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                                                     <  1:192425/140‑1 (MQ=255)
                                                                                       cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                                                     >  1:411822/1‑140 (MQ=255)
                                                                                       cGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTc                                                     <  1:106973/140‑1 (MQ=255)
                                                                                           gattTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGACTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatat                                                 <  2:2891437/140‑1 (MQ=255)
                                                                                           gaggTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCatat                                                 <  2:1662920/137‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:4252076/140‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:196156/140‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:2738979/140‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  1:213405/140‑1 (MQ=255)
                                                                                              gTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATAGTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtg                                              <  2:218518/140‑1 (MQ=255)
                                                                                                cGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTGTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATAtgtt                                            <  2:2873777/140‑1 (MQ=255)
                                                                                                              gCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTAATATATGTTGTCCACAATTGTgg                              >  2:3632665/1‑140 (MQ=255)
                                                                                                                  aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  2:697583/140‑1 (MQ=255)
                                                                                                                  aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  2:2669897/140‑1 (MQ=255)
                                                                                                                  aaaGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGatat                          <  1:2242133/140‑1 (MQ=255)
                                                                                                                        gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCAAATATGTTGTCCACAATTGTGGATATCTTTTc                    >  2:1109195/1‑140 (MQ=255)
                                                                                                                        gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATACCCACTTTAAAAAGAAGTTATTCATATATGTTGTCCACAATTGTGGATATCtttta                    >  1:2647772/1‑139 (MQ=255)
                                                                                                                        gTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCAAAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTc                    >  2:1973137/1‑140 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGTTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  2:2459766/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGTTATTTTTATTTTAGTTGTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:3632701/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACGGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:401909/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:1427927/140‑1 (MQ=255)
                                                                                                                              tgtATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTCATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTAtt              <  1:388984/140‑1 (MQ=255)
                                                                                                                                  ttcttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTtgt          >  1:2899132/1‑140 (MQ=255)
                                                                                                                                    cttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTgtgt        <  1:3392991/140‑1 (MQ=255)
                                                                                                                                     ttcAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCt                   <  1:2387134/128‑1 (MQ=255)
                                                                                                                                       cAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg     >  1:3991016/1‑140 (MQ=255)
                                                                                                                                       cAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg     >  1:2630733/1‑140 (MQ=255)
                                                                                                                                       cAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg     >  2:2556322/1‑140 (MQ=255)
                                                                                                                                       cAATGCCTTGCTTGAGGTATTTTTCTTTGAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg     >  1:3062596/1‑140 (MQ=255)
                                                                                                                                       cAATGCCTTGCGTGAGGTATTTTTCTTTTAGTTTTCCGATGCTCACAATATTGAGATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAg     <  2:2255879/140‑1 (MQ=255)
                                                                                                                                        aaTGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTaata    >  2:3059752/1‑138 (MQ=255)
                                                                                                                                        aaTGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGa    >  2:214417/1‑140 (MQ=255)
                                                                                                                                          tGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATc  >  1:590765/1‑140 (MQ=255)
                                                                                                                                          tGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATc  >  1:3160695/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TGGATAGGATGCGGTCGCCTTCTTTGTCTTTTATGATTTTCATGTCCTGGTCGCTTAGGTTTTCCGGCGCTTTTTCGTCCGGGAGTTCGATGATGTCGATTTTTGCGTAGGCCGAAAGTCGTTTCGTGTATTCTTCAATGCCTTGCTTGAGGTATTTTTCTTTTAGTTTTCCGATTGTCACAATATTGATATTCACAGGTCATCCCCACTTTAAAAACAAGTTATTCATATATGTTGTCCACAATTGTGGATATCTTTTCTGTATTTTGTGTAAGATC  >  NZ_CP034943/3976010‑3976287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: