Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,992,777 G→T 81.5% L295M (CTG→ATG)  walK ← cell wall metabolism sensor histidine kinase WalK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,992,7770GT81.5% 1190.6 / 241.4 521L295M (CTG→ATG) walKcell wall metabolism sensor histidine kinase WalK
Reads supporting (aligned to +/- strand):  ref base G (45/52);  new base T (212/212);  total (257/264)
Fisher's exact test for biased strand distribution p-value = 5.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 556 total aligned reads displayed.

AATTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCT  >  NZ_CP034943/3992641‑3992913
                                                                                                                                        |                                                                                                                                        
 aTTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAACTCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTcc                                                                                                                                      <  1:946861/140‑1 (MQ=255)
 aTTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTcc                                                                                                                                      >  1:3606560/1‑140 (MQ=255)
 aTTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGAAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTcc                                                                                                                                      >  2:2518735/1‑140 (MQ=255)
   tcatcaTCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAg                                                                                                                                    >  2:748459/1‑140 (MQ=255)
      tcatcCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAgcgc                                                                                                                                 >  2:2014675/1‑140 (MQ=255)
      tcatcCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAgcgc                                                                                                                                 >  2:1538206/1‑140 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCACGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  2:2166327/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  1:2184787/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  1:3284472/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             <  2:2102250/140‑1 (MQ=255)
          cccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCggg                                                                                                                             <  1:3366274/140‑1 (MQ=255)
           ccGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGc                                                                                                                            <  1:2927841/140‑1 (MQ=255)
                  aTTTCAAGCAGCATAGAATCCTGCTGCTCAACTAAATCTTCAAACGTATAATTCTCTAGAATACCCAGCAAGCTCGTAATCGGCAGTTCTAAGGCTGTTTCTCGTGAAACGTTCAGCATCTCCAGCTCCGGGCTGTTTac                                                                                                                     >  1:3910497/1‑139 (MQ=255)
                     tCAAGCAGCATAGAATCCTGCTGCTCCACTAACTCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAagag                                                                                                                  <  1:2077398/140‑1 (MQ=255)
                     tCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAagag                                                                                                                  <  1:2682497/140‑1 (MQ=255)
                             caTAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTg                                                                                                          >  1:1955809/1‑140 (MQ=255)
                             caTAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTg                                                                                                          >  1:1579420/1‑140 (MQ=255)
                               tAGAATCCTGCTGCTCCACTAAATCTTCCAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGcc                                                                                                        <  2:2472552/140‑1 (MQ=255)
                               tAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGcc                                                                                                        <  1:2755463/140‑1 (MQ=255)
                               tAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGcc                                                                                                        <  1:2562070/140‑1 (MQ=255)
                                    tCCTGCTGCTCCACTAAATCTTCAAACGTATAAGTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGtt                                                                                                   <  2:1467886/140‑1 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAATGGTGTTTCACGTGAAACGTTCAGCTTCTCCAGCGCCGGGCTGTGTATGAGAATAATTGCCCCGTTTCg                                                                                                >  1:2317193/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:4257889/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:4147561/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  1:332614/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:1239894/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:1192282/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:2845793/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  1:1627866/1‑140 (MQ=255)
                                       tgctgcTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCg                                                                                                >  2:321753/1‑140 (MQ=255)
                                         ctgctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGt                                                                                              >  2:3878852/1‑140 (MQ=255)
                                         ctgctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGt                                                                                              >  1:2400244/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  1:1428964/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  2:3022669/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  1:3423927/1‑140 (MQ=255)
                                            ctCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtg                                                                                           >  2:3728473/1‑140 (MQ=255)
                                             tCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTtgt                                                                                          <  2:640713/140‑1 (MQ=255)
                                                 cTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCg                                                                                      >  2:2797402/1‑140 (MQ=255)
                                                         tCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGcc                                                                              >  1:3756055/1‑140 (MQ=255)
                                                         tCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGcc                                                                              >  1:374414/1‑140 (MQ=255)
                                                         tCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGcc                                                                              >  1:2029549/1‑140 (MQ=255)
                                                         tCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGGTTGTGGCGATAACGcc                                                                              >  1:3630688/1‑140 (MQ=255)
                                                           aaaCGTATTATTCTCTTCAATCCCCAGAAAGCTCGTAATCGGCATTTCTAATGCTTTTTAACGTGAAACGTTCAGCATCTACAGCTCAGGGCTGTTTAAGATAATAATTTGCCAGTTTATGTTTTTGGCGATAACGcgtt                                                                            <  2:3295779/140‑4 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  2:352505/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  2:2075020/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  1:3490548/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  1:2819728/140‑1 (MQ=255)
                                                           aaaCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGt                                                                            <  2:3771845/140‑1 (MQ=255)
                                                             aCGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCt                                                                          <  1:2988901/140‑1 (MQ=255)
                                                                  taATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCat                                                                     >  2:91619/1‑140 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  2:1047141/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  2:3389525/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:1798385/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  2:1620494/140‑1 (MQ=255)
                                                                   aaTTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCTGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCata                                                                    <  1:2112809/140‑1 (MQ=255)
                                                                      tctcTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAg                                                                 <  1:613941/140‑1 (MQ=255)
                                                                      gctctTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAg                                                                 <  1:1713620/139‑1 (MQ=255)
                                                                       ctctTGAATCCCCAGCAAGCTCGGAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAgg                                                                <  2:2288014/140‑1 (MQ=255)
                                                                        tctTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGc                                                               <  1:737391/140‑1 (MQ=255)
                                                                        tctTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGc                                                               <  2:3606560/140‑1 (MQ=255)
                                                                         ctTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCa                                                              >  1:3879029/1‑140 (MQ=255)
                                                                         ctTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTGTGTGGCGATAACGCCGTCGGTCATATAGGCa                                                              >  2:2989693/1‑140 (MQ=255)
                                                                          ttGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             >  2:1267362/1‑140 (MQ=255)
                                                                          ttGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCaa                                                             >  1:4266668/1‑140 (MQ=255)
                                                                               tCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGGTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACa                                                        >  2:1352680/1‑140 (MQ=255)
                                                                                  ccAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCAtata                                                                  >  2:1748600/1‑127 (MQ=255)
                                                                                    aGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGc                                                   >  1:306463/1‑140 (MQ=255)
                                                                                    aGCAAGCTCGTAATCGGCATTTCTAAGGCTGTGTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGACTGATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGc                                                   >  1:1193622/1‑140 (MQ=255)
                                                                                              tAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTg                                         <  2:3681080/140‑1 (MQ=255)
                                                                                                aTCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATTGGCAATAACAGAGGCAAGCTTTCTgcg                                       <  2:2725922/140‑1 (MQ=255)
                                                                                                aTCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCACCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTgcg                                       <  2:1112922/140‑1 (MQ=255)
                                                                                                  cGGCATTTCTAAGTCTGTTTCACGTGACACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGct                                     <  1:816710/140‑1 (MQ=255)
                                                                                                  cGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGct                                     <  1:915208/140‑1 (MQ=255)
                                                                                                   ggCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCggg                                                                                                                             >  1:1908043/1‑51 (MQ=255)
                                                                                                    gCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGctct                                   >  2:1672511/1‑140 (MQ=255)
                                                                                                           tAAGGCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGATTAAGAGAATAATTGCCCCGGTTCTGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTg                            >  2:1931701/1‑140 (MQ=255)
                                                                                                              ggCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCa                         <  2:1823223/140‑1 (MQ=255)
                                                                                                               gCTGTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCAt                        >  2:855134/1‑140 (MQ=255)
                                                                                                                  gTTTCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGc                     >  1:1026426/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  2:399729/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  2:3070099/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    <  1:4019674/140‑1 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  2:3829873/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGGTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  1:1335464/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  1:2482393/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  1:4100847/1‑140 (MQ=255)
                                                                                                                   tttCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGcc                    >  1:3145413/1‑140 (MQ=255)
                                                                                                                    ttCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCt                   >  2:469450/1‑140 (MQ=255)
                                                                                                                     tCACGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCGGTCCTCGCCTg                  >  2:2958870/1‑140 (MQ=255)
                                                                                                                       aCGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                <  1:3807273/140‑1 (MQ=255)
                                                                                                                       aCGTGAAACGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                <  1:2257036/140‑1 (MQ=255)
                                                                                                                       aCGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTggg                <  1:1393576/140‑1 (MQ=255)
                                                                                                                          tGAACCGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCACTAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCAt             >  1:1494327/1‑140 (MQ=255)
                                                                                                                             aaCGTTCAGCATCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCtt          >  2:3190742/1‑140 (MQ=255)
                                                                                                                                   gagcatcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct    <  1:852747/139‑1 (MQ=255)
                                                                                                                                   cagcatCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct    <  1:561443/140‑1 (MQ=255)
                                                                                                                                   cagcatCTCCAGCGCCGGGCTGTCTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGct    <  1:2616028/140‑1 (MQ=255)
                                                                                                                                     gcatcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct  <  2:332925/140‑1 (MQ=255)
                                                                                                                                     gcatcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct  <  2:587869/140‑1 (MQ=255)
                                                                                                                                     gcagcTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGctct  <  2:2400244/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AATTCATCATCCCGCTCAATTTCAAGCAGCATAGAATCCTGCTGCTCCACTAAATCTTCAAACGTATAATTCTCTTGAATCCCCAGCAAGCTCGTAATCGGCATTTCTAAGGCTGTTTCACGTGAAACGTTCAGCAGCTCCAGCGCCGGGCTGTTTAAGAGAATAATTGCCCCGTTTCGGTTTGTGGCGATAACGCCGTCTGTCATATAGGCAATAACAGAGGCAAGCTTTCTGCGCTCTCCTTCTGTCATCGCCTGGGCATCTTCAAGCTCT  >  NZ_CP034943/3992641‑3992913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: