Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,838 G→T 7.3% intergenic (‑144/+62) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8380GT 7.3% 753.8 / 15.6 265intergenic (‑144/+62)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base G (124/115);  new base T (2/24);  total (126/139)
Fisher's exact test for biased strand distribution p-value = 1.27e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

TGCACCTCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAT  >  NZ_CP034943/3996700‑3996976
                                                                                                                                          |                                                                                                                                           
tGCACCTCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGt                                                                                                                                            >  2:3095369/1‑140 (MQ=255)
tGCACCTCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGt                                                                                                                                            >  1:1717718/1‑140 (MQ=255)
tGCACCTCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGt                                                                                                                                            >  1:2813090/1‑140 (MQ=255)
     cTCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTtct                                                                                                                                       >  1:1576200/1‑140 (MQ=255)
     cTCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTGTTACACGCGTTTtct                                                                                                                                       >  1:59382/1‑140 (MQ=255)
      tCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTGTTACAAGCGTTTtcta                                                                                                                                      >  1:1230449/1‑139 (MQ=255)
       ccGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCa                                                                                                                                     >  2:763233/1‑140 (MQ=255)
       ccGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCa                                                                                                                                     >  2:461669/1‑140 (MQ=255)
       ccGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCa                                                                                                                                     <  1:982786/140‑1 (MQ=255)
       ccGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCa                                                                                                                                     >  2:1781464/1‑140 (MQ=255)
       ccGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCa                                                                                                                                     >  2:120471/1‑140 (MQ=255)
       ccGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACAGTAAATTAATGTAAATCAATATTCGTTCGCTTCAATTCGTTTTACAAGAGTTTTCTCa                                                                                                                                     >  2:3900590/1‑140 (MQ=255)
       ccGTTAACCTTTCAAAACGATTCTATTAAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTACTGTAAATAAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCa                                                                                                                                     <  1:2474307/140‑1 (MQ=255)
          ctAACCTTTCAAAACGATTCTATTCACACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAAGATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCg                                                                                                                                  <  2:2807158/139‑1 (MQ=255)
            acccTTTCAAAACGATTCTATTCAAACAAATCAAGTGTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCACTATTCGTGCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  1:4128903/138‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCACACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCACTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTGTTCTCATCGCa                                                                                                                                <  1:2428001/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAACTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  2:3155719/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  1:3854480/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  1:3014204/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  2:2326341/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  1:104117/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  2:1150353/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  2:988730/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  1:2043821/140‑1 (MQ=255)
            aaCCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  1:1176538/140‑1 (MQ=255)
            aaCCTGTCAAAACGATTCTATTCAAACAAATCAACTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  1:3056278/140‑1 (MQ=255)
            aaCCCTTCCAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCa                                                                                                                                <  2:2258029/140‑1 (MQ=255)
                  tCAAAACGATTCTGTTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                          >  2:4011623/1‑140 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTcc                                                                                                                          >  1:492080/1‑140 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                          >  1:1522245/1‑140 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                          >  2:2197188/1‑140 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTca                                                                                                                          >  2:1849832/1‑139 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGGTCGCTTCCATTCGTTTTACAATCGTTTTATCATCGCATTGTcc                                                                                                                          >  1:996079/1‑140 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATAAATATTCGTTCGCTTACATTCGTTTTACAAGCGTTTTCTCATCGCATTGtgc                                                                                                                          >  2:3170754/1‑138 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATGAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCAGCGCAgtgtgg                                                                                                                          >  1:3686690/1‑138 (MQ=255)
                  tCAAAACGATTCTATTCAAACAAATAAAGTTTATCATTGAAGAAACAGAAAGTAAATTGTAAATCCGAACAATAAATTAATGTAAATCAATATTCGTTCGCTTCAATTCGTTTTACAAGCGTTTTCTAATCGCATTGTcc                                                                                                                          >  2:665567/1‑140 (MQ=255)
                  tCAAAACGATTATATTCAAACAAATAAAGTTTAACAGACAAGAAACAGAAAGACAAATATAAAAACGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTAAAAGCGTTTTCTCATCGCATTGTcc                                                                                                                          >  2:1566435/1‑140 (MQ=255)
                         gATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAg                                                                                                                   >  1:3433315/1‑140 (MQ=255)
                           ttCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGcg                                                                                                                 >  2:3629500/1‑139 (MQ=255)
                                ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                            <  1:1491368/140‑1 (MQ=255)
                                     acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                       >  1:3985887/1‑140 (MQ=255)
                                      caacTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACttt                                                                                                      <  1:313549/140‑1 (MQ=255)
                                      caaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTCTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACttt                                                                                                      <  1:802730/140‑1 (MQ=255)
                                       aaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtttt                                                                                                     <  1:130769/140‑1 (MQ=255)
                                          tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                  >  1:1132899/1‑140 (MQ=255)
                                                 tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                           <  1:4097571/140‑1 (MQ=255)
                                                  aTCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCa                                                                                          <  1:3520382/140‑1 (MQ=255)
                                                    cAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACg                                                                                        <  2:3257476/140‑1 (MQ=255)
                                                            aaaCAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCTCGAAAAagag                                                                                >  2:2831936/1‑140 (MQ=255)
                                                                 gAAAGTCAATTGTACATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAATAGAGTTACt                                                                          <  2:3873977/140‑1 (MQ=255)
                                                                 gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                          >  1:460248/1‑140 (MQ=255)
                                                                  aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                         <  1:2591578/140‑1 (MQ=255)
                                                                  aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                         <  1:779976/140‑1 (MQ=255)
                                                                  aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                         <  1:3576020/140‑1 (MQ=255)
                                                                  aaaGTCAAGTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                         <  1:2924522/140‑1 (MQ=255)
                                                                   aaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTg                                                                        >  1:3321375/1‑140 (MQ=255)
                                                                    aGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                       <  1:863604/140‑1 (MQ=255)
                                                                         aTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGat                                                                  <  2:338339/140‑1 (MQ=255)
                                                                          ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                 <  2:1430673/140‑1 (MQ=255)
                                                                          ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTGAGCACGAAAAAGAGTTACTTGCCTGata                                                                 <  1:289809/140‑1 (MQ=255)
                                                                            gTAAACCCGAACTTTAAATTAATGTAAATCAATTTTCGTCCGCTCCCTTCCTTTTTACACGGTTTTTCTCTTCGCATTGTCCTAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATcc                                                              <  1:371911/140‑1 (MQ=255)
                                                                                aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                           >  1:2559592/1‑140 (MQ=255)
                                                                                     aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                      <  1:2197188/140‑1 (MQ=255)
                                                                                     aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                      >  1:2120051/1‑140 (MQ=255)
                                                                                     aaCATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTACCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTa                                                      <  1:2718887/140‑1 (MQ=255)
                                                                                          taaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                           <  2:1590192/99‑1 (MQ=255)
                                                                                            aattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTCTTCATaa                                               >  1:742877/1‑140 (MQ=255)
                                                                                                  tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                         >  1:2841564/1‑140 (MQ=255)
                                                                                                  tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                         >  1:551745/1‑140 (MQ=255)
                                                                                                  tGTAAATCAATATTCGTTCGCTCCCATTCTTTTTACAAGCGTTTTGACTTCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGa                                         <  2:2067809/140‑1 (MQ=255)
                                                                                                       aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCGTTTTGTATTCATAAAAACGACTctc                                    >  2:2489491/1‑138 (MQ=255)
                                                                                                       aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGc                                    >  2:2688311/1‑140 (MQ=255)
                                                                                                            tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                               >  2:876576/1‑140 (MQ=255)
                                                                                                            tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                               >  2:336692/1‑140 (MQ=255)
                                                                                                                 gttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCg                          >  1:180521/1‑140 (MQ=255)
                                                                                                                  ttggCTTCCATTGTTTTTACAAGTTTTTTATCTTCGCTTTTTCCAAAAAAGCTCGTGAAACTTTTTTCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                         <  2:2587662/137‑1 (MQ=255)
                                                                                                                  ttcgTTTCATTTCGTTTTAAAAGCTTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAATTTTTATCATTTAGCACCAAAAAGTTTTAGTTGCCTTATATCCCTTTTTTATCCATAAAAACGACTCGCCAACCGTCCgg                         <  2:3856363/140‑1 (MQ=255)
                                                                                                                  ttcgATTCCATTGTTTTTAAAAGCTTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGTGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGACTCGCCAACCGTCCgg                         <  2:4081655/140‑1 (MQ=255)
                                                                                                                    cgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGAt                       >  1:1901459/1‑140 (MQ=255)
                                                                                                                     tcTTCCTTTTTTTTTACACGGTTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTTAAATTTTTATCCTTTAGCACTAAAAAGAGGTACTTGCCTGATATCCGTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATc                      <  2:3709613/139‑1 (MQ=255)
                                                                                                                     gCTTCCTTTCTTTTTACAAGCGTTTTCTCATCTCATTTTCATAAAAAGCTCTTGAAACTTTTATCCTTTAGCACTAAAAAGATTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATc                      <  2:1248510/140‑1 (MQ=255)
                                                                                                                      cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                     >  2:1031353/1‑140 (MQ=255)
                                                                                                                       ttacATTCGTTTTACAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTGGTTAAACTTTTATCCTTTAGCACTAAAAAGTGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCct                    <  2:3797286/137‑1 (MQ=255)
                                                                                                                            ttCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATc               >  2:3501513/1‑140 (MQ=255)
                                                                                                                            ttCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATc               >  2:1654671/1‑140 (MQ=255)
                                                                                                                              ctttttACAAGGTTTTTCACATCGAATTGTCCTAAAATCCCCGTTAAACTTTCACCCTTCACCCCCAAAAACTTTACGGTGCATGATATCAGTTTTTTTTTAATAACAACGACTCGCCAACCGTCCGGATCCTCTATCGt             <  1:1602224/138‑1 (MQ=255)
                                                                                                                              ctttttACAAGGGTTTTATCATCGAATTGTCCTAAAAAGCTGTTGAAACTTTTATCCTTTAGCACTAAAAATAGTTACTTGCCTCTTATCCGTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt             <  2:2444078/138‑1 (MQ=255)
                                                                                                                              ctttttACAAGCGTTTTCTCATCGAATTGTCCTAAAAAGCTCGTCAAACTTTTAGCCTTTAGCACGAAAAAGTGTTACTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt             <  2:1318208/138‑1 (MQ=255)
                                                                                                                                ttttACATGGTTTTTCTAATCGCTTTGTAAAAAAACGCTCGTGAAATTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa           <  2:3037975/140‑1 (MQ=255)
                                                                                                                                ttttACAAGCGTTTTCTCATCTCATTTTCATAAAAAGCTCGTTAAACTTTTATCTTTTAGAACGAAAAAGAGTTACTTGCCTTATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa           <  2:595104/140‑1 (MQ=255)
                                                                                                                                ttttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa           >  2:1937045/1‑140 (MQ=255)
                                                                                                                                 tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc          >  2:2984121/1‑140 (MQ=255)
                                                                                                                                 tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc          >  1:3818583/1‑140 (MQ=255)
                                                                                                                                   tataaGCGTTTTCTCACTGCTTTGTCATAAAAAGCTTGCGAAACTTTCATCCTTGAGCATAAGAAAGAATCACTTGCCTGATATCCCTTTTGAATTCATAAAAACGATCCGCCAGCCGTCCGGGTCCTCTATCGTCACGc        <  2:291974/137‑1 (MQ=255)
                                                                                                                                   tACAAGGGTTTTATCTTGGCTTTGTCATAAAATGCTCGTTAAACTTTTATCCTTTACCACTAAAACTAGTTACTGGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc        <  2:1722725/140‑1 (MQ=255)
                                                                                                                                        gggTTTTCTAAGCGCTTTGTCCAAAAATCCTCGTCAAAGTTTTAACCTTTAGCACTAAAAAGAGTTCGTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa   <  1:4011623/138‑1 (MQ=255)
                                                                                                                                        gggTTTTATCATCGCATTGTCATAAAAAGCTCTTCAAACTTTTATCGTTTAGCACGAAAAAGAGTTACGTGCCTGATAGCCCTTTTGTATGCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa   <  2:3570680/138‑1 (MQ=255)
                                                                                                                                         ggTTTTCTCATCGCTTTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTCCCACTAAAAAGAGTTAGTTGCCGGATATCCCTTTTGTTTCCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt  <  2:4036074/139‑1 (MQ=255)
                                                                                                                                         ggTTTTATCATCTCATTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTACCACTAAAAAGAGTTACTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt  <  2:492080/139‑1 (MQ=255)
                                                                                                                                         cGTTTTCTCATCGAATTGTCCTAAAAAGCTCGTTAAAATTTTATCCTTTACCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt  <  2:4159699/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
TGCACCTCCGTTAACCTTTCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAT  >  NZ_CP034943/3996700‑3996976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: