Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 316,262 C→A 10.4% Q28K (CAA→AAA)  opuAA → glycine/proline betaine ABC transporter ATP‑binding protein OpuAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943316,2620CA10.4% 693.5 / 11.2 303Q28K (CAA→AAA) opuAAglycine/proline betaine ABC transporter ATP‑binding protein OpuAA
Reads supporting (aligned to +/- strand):  ref base C (163/94);  new base A (2/42);  total (166/137)
Fisher's exact test for biased strand distribution p-value = 2.12e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

GTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316123‑316401
                                                                                                                                           |                                                                                                                                           
gtgtgGAATGTTACATAAGTGTTACGGTTAGAAGGATTGTTTAATAGGGAGGGAAACTATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTc                                                                                                                                             <  2:3591421/140‑1 (MQ=255)
   tgGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAACTCGAGAAAGTATCTATAATTTTTGGGAAACAATCTAGGCACGGAGTTCaaa                                                                                                                                          >  2:3637037/1‑140 (MQ=255)
       aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAACCAAACAAAGAAAGCAGTTCAAATGCt                                                                                                                                      >  1:2283780/1‑140 (MQ=255)
       aTGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCt                                                                                                                                      >  1:3859246/1‑140 (MQ=255)
                aaGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACgg                                                                                                                             >  1:2498371/1‑140 (MQ=255)
                     ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTATGATAAAAGTCGATAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  2:1989986/1‑140 (MQ=255)
                     ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  2:3271531/1‑140 (MQ=255)
                     ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  2:202953/1‑140 (MQ=255)
                     ttACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGa                                                                                                                        >  2:256096/1‑140 (MQ=255)
                       aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACa                                                                                                                      >  1:3082478/1‑140 (MQ=255)
                       aCGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACAAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGTAAACAAACAAATAAAGCAGTTCAAATGCTTGACAACCGAAAGACa                                                                                                                      >  1:763915/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCACATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:1049211/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGCAAGGCaaaaaag                                                                                                                >  2:2156123/1‑139 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACataaaaa                                                                                                                >  2:2079701/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:890048/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:1745619/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:4009225/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:1221168/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:2627009/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  2:3381512/1‑140 (MQ=255)
                             ataAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACaaaaaaa                                                                                                                >  1:2943534/1‑140 (MQ=255)
                                aGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAagag                                                                                                             <  1:963983/140‑1 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATTCTTTCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:2434201/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGACAGACACCAAAAGAGCTccg                                                                                                        >  1:2295909/1‑139 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:3398752/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:3627184/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:1766327/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:344047/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  1:2547047/1‑140 (MQ=255)
                                     tGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCt                                                                                                        >  2:3890206/1‑140 (MQ=255)
                                       tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  1:1608332/1‑140 (MQ=255)
                                       tttAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGa                                                                                                      >  2:1524921/1‑140 (MQ=255)
                                            tatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCg                                                                                                 >  1:3445474/1‑140 (MQ=255)
                                            tatGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCg                                                                                                 >  1:3502315/1‑140 (MQ=255)
                                              tgtagtcaaaaTATTAGTGTAGATCAGAAACAAATTACGATAAAAGTCGATAAAGTATCTAAAATTTTTGGGAAAAAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAACGCGAc                                                                                               <  2:1030427/133‑1 (MQ=255)
                                              tGGAGGTAAAATATGAGTTTATATTACAAACAAATTAAGATAAACGTCGACAAATTATCTAAAATTTTTGGTAAACAAAAAAATAAAGCAGTTAAAATGCTTGCCAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGAc                                                                                               <  2:3484996/140‑1 (MQ=255)
                                              tGGAGGCAACATATCCGGGTATATGAGACTCCATTTAAGATAAAAGTCGAGAACGTATCAAAAATTTTTGGCAAACAAACAAAGAAACCAGTTAAAATGCTTGCCAACGCAAAGAAAAAAAAAGAGATCATGAAAGCGAc                                                                                               <  2:2861757/140‑1 (MQ=255)
                                              tGGAGGCAAAATATGAGTGTAGATGATAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAACAAAGCAGTTAAAATGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAACGCGAc                                                                                               <  2:836032/140‑1 (MQ=255)
                                                 aGGGAAAATATGAGTGTATATCAGAAACCAATTAAGATAAAAGTCGAGAAACTATCTAAAATTTTTGGGAAAAAAACAAAGAAAGCATTTAAAATGCTTGCCAAACTAAAGACAAAAAAAGAGATCCTGAAAGCGAcacg                                                                                            >  1:4127791/1‑137 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGACACAAACAAAGAAAGCAGTTCAAATGCTTGCGAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:3557787/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:724115/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  1:826866/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCgg                                                                                            >  2:2374067/1‑140 (MQ=255)
                                                 aGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGATTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGAGACCgg                                                                                            >  1:3756197/1‑140 (MQ=255)
                                                  gggAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGa                                                                                           >  1:3712150/1‑140 (MQ=255)
                                                   ggAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGAGAcatgat                                                                                          >  2:2487920/1‑135 (MQ=255)
                                                         tatGAGTGTTGATGACAAACAAATTAAGATAAAAGTCCAGAAAGTATCTAAAATTTTTGGAAAACAAACAAACAACCCCGTAAAATTCATTGCCACCGCAAACAAAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCg                                                                                    <  1:2413990/140‑1 (MQ=255)
                                                               tttAGATGAGAAACAAATTAAGATAAACGTCGACAAACTATCTAAAATTTTTGGGAAACAAAAAAACAAAGAAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              <  2:920092/138‑1 (MQ=255)
                                                               tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  1:1987139/1‑140 (MQ=255)
                                                               tgtAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTgggg                                                                              >  2:2580793/1‑140 (MQ=255)
                                                                gtAGATGAGAAACCAATTAAGATAAACGGCGCCAAAGTATCTAAAATTTTGGGGAAACAAACAAAGAACGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGt                                                                             <  2:739137/140‑1 (MQ=255)
                                                                 tAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTa                                                                            >  2:1666577/1‑140 (MQ=255)
                                                                   gATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaaa                                                                          >  1:2068932/1‑140 (MQ=255)
                                                                   gATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaaa                                                                          >  1:1857957/1‑140 (MQ=255)
                                                                   gATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTaaa                                                                          >  1:476589/1‑140 (MQ=255)
                                                                        taaaCCAATTAAGATAAAAGTCTAGAAAGTATATAAAATTTTTGCGAAACAAACAAAGAAAGCCGTTCAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAg                                                                     <  2:3068948/139‑1 (MQ=255)
                                                                         aaaCCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGc                                                                    >  2:2279267/1‑140 (MQ=255)
                                                                                ttAAGAAAAAAGGCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTAAAAGGGTTCCCAACGGAAAGACAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGAtttt                                                             <  2:2736911/140‑1 (MQ=255)
                                                                                 taacaaaaacGTCGAGAAATTATATAAATTTTTGGGCAAAAAAACAAACAAACCATTTCAAAGGCTTGCCAACGCAAACACAAAAAAAGACATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  1:1011810/134‑1 (MQ=255)
                                                                                 tAAGATAAACGTCGAGAAAGTCTCTAAGATTTTTGGGAAACAAACAAAGAAGGCAGTTCAAATGCGTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCAACCGGATCAACCGTTGGGGTTAATCAGGCAGATTTTg                                                            <  2:804103/140‑1 (MQ=255)
                                                                                 tAAGATAAAAGTCGAGAAAGTATCTAAATTTTTTGGGAAACAAAAAAAGAAAGCAGTTCAACTGCTTGCCAACGTAAAGACAAAAAAACAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:2989293/140‑1 (MQ=255)
                                                                                 tAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAACTGCTTGCCACCGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:1217243/140‑1 (MQ=255)
                                                                                 tAACATAAAAGTCGAGAAATTAACTAAAATTTTTGGGAAAAAAACAAAGAAAGCAGTAAAAATGCTTGCCACCGCAAAGAAAAAAAAAGTGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTg                                                            <  2:1857957/140‑1 (MQ=255)
                                                                                   atacaaaaGTCGAGAAAGTATAAAAAATTTTTGCCAAAAAAAAAAAGAAAGCAGTACAAATGCTTGCCAACGCAAACAAAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:3252186/136‑1 (MQ=255)
                                                                                   ataTAACAGTCGACAAATTAAAAAAAATTTTTGGGAAAAAAACAAACAAAGCAGTACAAAGGCTTGCCAACGCAAACACAAAAAAAGAGATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:4224626/138‑1 (MQ=255)
                                                                                   ataTAAAAGTCTACAAATTATCTAAAATTTTGGCCAAAAAAAAAAACAAACCAGTAAAAATGCTTCCCAACGCAAACAAAAAAAAAGACATCCTCAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  1:202953/138‑1 (MQ=255)
                                                                                   aGAAAAAAGCCGACAAAGAATCTAAAATTTTTGCCAAACAAAAAAACAAAGCAGTTCAAATGCTTCCCACCGCAAACAAAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGAt                                                          <  2:231472/140‑1 (MQ=255)
                                                                                       aaaaGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtat                                                      >  1:1325868/1‑140 (MQ=255)
                                                                                        aaaGTCGATAAATTATCTAAAATTTTTGGCAAACAAACAAAGAAAGCCGTTCAAATGCTTGCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGtata                                                     <  2:476589/140‑1 (MQ=255)
                                                                                              gagaAAGTATCTAAAATTTTTGGGAAACAAACAAATAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATgg                                               >  1:4143984/1‑140 (MQ=255)
                                                                                               acaatgTATCTAAAATTTTTGGTAAACAAACAAAGAAAGCAGTAAAAAGGTTTGCCACCGCAAACAAAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGt                                              <  1:603465/135‑1 (MQ=255)
                                                                                                      ctCTAAAATTTTGGCGAACCAACCAAATAACTCAGTGCACAGCCTTGCCACCCCAAACACAAAAAAAGAGTTCCTTAACGCGACCGGATCAACCGTTGGGGTAAATCACGCAGATTTTGTTGTATATGATGGTGAGatat                                       <  2:3251833/139‑1 (MQ=255)
                                                                                                      atatataaTTTTTGGGAAACATACAAAGAACTCATTTAAAATGCTGGCAAACGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       <  2:4127791/134‑1 (MQ=255)
                                                                                                      atatAAAATTTTTGGGAAACAAACTAAGAAAGCACTTCAAATGGTTGCCAACTGAATCACAAAAAAAGACAGCGTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGatat                                       <  2:1457450/137‑1 (MQ=255)
                                                                                                        atAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTAAAATGCTTGCCACCGGAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                     <  2:3858721/139‑1 (MQ=255)
                                                                                                        atAAAATTTTTGGCAAACAAACAAATAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGATATCCTGACAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATAttt                                     <  2:2806133/139‑1 (MQ=255)
                                                                                                         tAAAATTTTTGGTAAACAAACAAAGAAAGCAGTTCAACTGCTTGCCAACGGAAACAAAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTg                                    <  1:2898726/140‑1 (MQ=255)
                                                                                                         tAAAATTTTTGGCAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTg                                    <  2:4225526/140‑1 (MQ=255)
                                                                                                          aaatTTTTTGGCAAAAAAAAAAATAAAGCATTCCAATTGGTGCCCAACGAAAACAAAAAAAAACAGACCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGt                                   <  1:1524921/140‑1 (MQ=255)
                                                                                                                tttGGTAAACAAACAAAGAACGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:1308039/140‑1 (MQ=255)
                                                                                                                tttGGGAAACAAACAAAGACAGCCTTTCAAATGGTTGCCAACGGAAAGACAAAAAAAGAGACCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:763915/140‑1 (MQ=255)
                                                                                                                tttGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATCGTGAGATATTTGTcatcat                             >  2:2523064/1‑140 (MQ=255)
                                                                                                                tttGGGAAACAAACAAAGAAAGCAGTACAACTGCTTGCCACCGCAAACACAAAAAAAGACATCCAGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:3929580/140‑1 (MQ=255)
                                                                                                                tttGGGAAACAAAAAAATAAAGCATTTAAAATGCTTGCCAACGTAACGAAAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  2:1971372/140‑1 (MQ=255)
                                                                                                                tttGGCAAACAAAAAAATAAACAAGTAAAACTGCTTGCAAACGTAAAGAAAAAACAAGAGAACCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTcatcat                             <  1:1680411/140‑1 (MQ=255)
                                                                                                                  tGGTAAAAAAAAAAACAAAGCAGTTCAAATGCTTGCCAACGCAAACAAAAAAAAAGAGATCCTTAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  2:3756197/140‑1 (MQ=255)
                                                                                                                  tGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  2:4013770/140‑1 (MQ=255)
                                                                                                                  tGGCAAACAAACAAATAAAGCAGTTAAAATGCTTGCCAACGCAAACAAAAAAAAAGACATCATGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:515547/140‑1 (MQ=255)
                                                                                                                  tGGCAAAAAAAAAAAGAAACCAGTTCAAACGCTTGCCAACGCAAACACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATgg                           <  1:2135033/140‑1 (MQ=255)
                                                                                                                    gcaaacaaaaAAACAAACCAGTTCAATTGCTTGCCAACGGAAACACAAAAAAAGAGAGCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                         <  1:2820109/138‑1 (MQ=255)
                                                                                                                    gcaaaaaaacAAACAACGCCGTAAAAATGCTTCCCACCGGAACCAAAAAAAACCCGAACCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                         <  1:3306338/138‑1 (MQ=255)
                                                                                                                    gcaaaaaaacAAACAAAGCATTTAAAATGCTTGCCAACGGAAAGAAAAAAAAAGAGATCCTGAACGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGt                         <  1:1815949/138‑1 (MQ=255)
                                                                                                                           agcaaaGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAg                  >  1:2320071/3‑140 (MQ=255)
                                                                                                                              aaagaaagCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGa               >  2:588572/1‑140 (MQ=255)
                                                                                                                              aaagaaagCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGa               >  2:82606/1‑140 (MQ=255)
                                                                                                                                   aagCATTTCAAATGCTTCCAAACGCAAACAAAAAAAAAGATATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGt          <  1:2747761/140‑1 (MQ=255)
                                                                                                                                     ccAGTTCAAATGCTTGCCAACGTAAACACAAAAAAATAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTaa        <  2:15926/139‑1 (MQ=255)
                                                                                                                                       aGTTCAAATGGTTGCCACCGCAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAAt      <  1:1027220/140‑1 (MQ=255)
                                                                                                                                        gTTCAAATGCTTGCCAACGTAAACAAAAAAAAAGAGATCCTCAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     <  2:3089671/140‑1 (MQ=255)
                                                                                                                                        gTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATc     >  2:2899870/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTGTGGAATGTTACATAAGTGTTACGGTTATAAGGATTGTTTAATATGGAGGGAAAATATGAGTGTAGATGAGAAACCAATTAAGATAAAAGTCGAGAAAGTATCTAAAATTTTTGGGAAACAAACAAAGAAAGCAGTTCAAATGCTTGCCAACGGAAAGACAAAAAAAGAGATCCTGAAAGCGACCGGATCAACCGTTGGGGTAAATCAAGCAGATTTTGATGTATATGATGGTGAGATATTTGTCATCATGGGTCTATCAGGGAGCGGTAAATCAAC  >  NZ_CP034943/316123‑316401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: