Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,996,857 C→A 9.1% intergenic (‑163/+43) purA ← / ← EO946_RS20530 adenylosuccinate synthase/VOC family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,996,8570CA 9.1% 470.6 / 10.8 200intergenic (‑163/+43)purA/EO946_RS20530adenylosuccinate synthase/VOC family protein
Reads supporting (aligned to +/- strand):  ref base C (76/99);  new base A (2/23);  total (78/122)
Fisher's exact test for biased strand distribution p-value = 3.73e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTC  >  NZ_CP034943/3996718‑3996995
                                                                                                                                           |                                                                                                                                           
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                             >  2:3869755/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                             >  1:1522245/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTAGTTTTACAAGCGTTTTCTCATAGCATTGTcc                                                                                                                                             >  1:2918870/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAAATGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTACATTCGTTTTACAAGCGTTTTCTCATCGCATTGTcc                                                                                                                                             >  2:3702683/1‑140 (MQ=255)
tCAAAACGATTCTATTCAAACAAATAAAGTTTATCATTGAAGAAACAGAAAGTAAATTGTAAATCCGAACAATAAATTAATGTAAATCAATATTCGTTCGCTTCAATTCGTTTTACAAGCGTTTTCTAATCGCATTGTcc                                                                                                                                             >  2:665567/1‑140 (MQ=255)
       gATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAg                                                                                                                                      >  1:3433315/1‑140 (MQ=255)
         ttCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGcg                                                                                                                                    >  2:3629500/1‑139 (MQ=255)
         ttCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAAAATTAACTTAATGTAAATCACTATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTTTCCTAAAAAGCt                                                                                                                                    >  2:1795573/1‑140 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                               <  1:1491368/140‑1 (MQ=255)
              ttCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGa                                                                                                                               <  2:1035677/140‑1 (MQ=255)
                   acaaaTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACtt                                                                                                                          >  1:96543/1‑140 (MQ=255)
                    caacTCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACttt                                                                                                                         <  1:313549/140‑1 (MQ=255)
                        tCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATc                                                                                                                     >  1:3050561/1‑140 (MQ=255)
                               tATCAGTGAAGAAACATAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                              <  1:1963194/140‑1 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                              >  1:603266/1‑140 (MQ=255)
                               tATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGc                                                                                                              <  1:4097571/140‑1 (MQ=255)
                                       aagaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGaaaaa                                                                                                      >  1:3697330/1‑140 (MQ=255)
                                        agaaACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAag                                                                                                     <  1:517943/140‑1 (MQ=255)
                                          aaaCAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCTCGAAAAagag                                                                                                   >  2:2831936/1‑140 (MQ=255)
                                          aaaCAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTGTAGCACGAAAAagag                                                                                                   >  2:2282290/1‑140 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                             >  1:3988266/1‑140 (MQ=255)
                                               gAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACt                                                                                             <  2:125614/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                            <  1:2591578/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                            <  2:3829014/140‑1 (MQ=255)
                                                aaaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACtt                                                                                            <  1:3576020/140‑1 (MQ=255)
                                                aaaGTCAAATGTAAATCCGAACATTTAATGAATTTAAATCAATATTCGTTAGCTTGAATTCGTTTTACAAGCGTTTTCTCATCGCATTGTACTAAAAAGCTCGTGAAACTTTTATCCATTAGCACGAAAAAGAGTTACtt                                                                                            <  1:2800232/140‑1 (MQ=255)
                                                 aaGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTg                                                                                           >  1:3321375/1‑140 (MQ=255)
                                                  aGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGc                                                                                          <  1:863604/140‑1 (MQ=255)
                                                    tCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCt                                                                                        >  1:3850461/1‑140 (MQ=255)
                                                       aTTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGat                                                                                     <  2:338314/140‑1 (MQ=255)
                                                        ttGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGata                                                                                    <  1:461669/140‑1 (MQ=255)
                                                         tGTAACTCCGAACAGTAAAATAATGTAAATCACTATTCGTTCGCTTCCATTAGTTTTACAATCGTTTTCTCATCGCATTTTCCTAACAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  2:2448441/140‑1 (MQ=255)
                                                         tGTAAATCCTAAAATTAAATGAATGTAAATCAATATTCGTTCGCTCCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  2:2229836/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  1:3074799/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  1:212074/140‑1 (MQ=255)
                                                         tGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGatat                                                                                   <  2:90695/140‑1 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAATCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTATCACGAAAAAGACTTACTTGCCTGATATCCCtt                                                                              >  1:822291/1‑140 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                              >  1:2559592/1‑140 (MQ=255)
                                                              aTCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTGTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCtt                                                                              >  1:4159699/1‑140 (MQ=255)
                                                                                tttaaataaaTATTGGTTCGCTTCATTTCGTTTTAAAATCTTTTTCTAATCGCATTGTCCTAAAAAGCTGGTCAAAATTTTATACTTTACAACTAAAAAGTGTTATTTGCCTTATACCCGTTTTTTTTTAATAAAAACGa                                                            <  2:1345127/138‑1 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                            >  2:352594/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                            >  1:1091543/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                            >  1:3456120/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                            >  1:2841564/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGa                                                            >  1:1582061/1‑140 (MQ=255)
                                                                                tGTAAATCAATATTCGTTCGCTCCCATTCTTTTTACAAGCGTTTTGACTTCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTAATAAAAACGa                                                            <  2:2067809/140‑1 (MQ=255)
                                                                                    aaTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGCACGAAAAAGAGTTACTTGCCTGATATCCCTTGTGTATTCATAAAAACGACTCg                                                        >  2:320610/1‑140 (MQ=255)
                                                                                     aTCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCGTTTTGTATTCATAAAAACGACTctc                                                       >  2:2489491/1‑138 (MQ=255)
                                                                                       aaaTATTCTTTCGCTTCCATTCTTTTTAAATGCTTTTTCTCATCGCTTTGTCATAAAAAGATCGTGAAACTTTTATCCTTTAGCACTAAAAAGTTTTATTTGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCa                                                     <  2:3012709/139‑1 (MQ=255)
                                                                                          tatTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAAcc                                                  >  2:876576/1‑140 (MQ=255)
                                                                                               gttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCg                                             >  1:180521/1‑140 (MQ=255)
                                                                                                tgcgCTTCAATTCTTTTTAAACGATTTTTCTCATCGATTTGTCCTAAAAAGCTCGTGAAATTTTTTTCCTTTTCAACGAAAAATATTTACTTGCCTTTTATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                            <  2:1241263/138‑1 (MQ=255)
                                                                                                 tcgCTTACATTCTTTTTAAAAGCGTTTTATAATCGCATTGTCATAAAAAGCTCGTGAAAATTTTATCCTTTACCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGa                                           <  2:2411596/140‑1 (MQ=255)
                                                                                                 tcgCTACCATTCGTTTTACAAGCTTTTTCTCATCGCATTTTCATAAAAAGCTCGTTAAAATTTTATCCTTTAGCACGAAAAATAGTTAGTTGCCTTATATCCATTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGa                                           <  2:1013699/140‑1 (MQ=255)
                                                                                                  cgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGAGTCGCCAACCGTCCGGAt                                          >  1:2285761/1‑140 (MQ=255)
                                                                                                    cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                        >  2:2708466/1‑140 (MQ=255)
                                                                                                     ttacATTCGTTTTACAAGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTGGTTAAACTTTTATCCTTTAGCACTAAAAAGTGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCct                                       <  2:3797286/137‑1 (MQ=255)
                                                                                                     tcacATTCTTTTTAAATGTTTTTACTCCTCGCTTTGTCAAAAAAAGCTCGTTAAATTTTTATCCTTTACCAGAAAAACTTGTTCCTTGCCTTATATCCTTTTTTTATTCATAAACACGACTCGCCAACCGTCCGGATCct                                       <  1:770633/137‑1 (MQ=255)
                                                                                                          ttCGTTTTACAATCGTTTTCTCATGGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCACTTTTGTATTCATAAAAAAGACTCTACAACAGTCCGGATCGTCTATc                                  >  2:3098179/1‑140 (MQ=255)
                                                                                                          ttCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAGAAACGACTCGCCAACCGTCCGGATCCTCTATc                                  >  1:2895154/1‑140 (MQ=255)
                                                                                                          ttCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATc                                  >  2:2823294/1‑140 (MQ=255)
                                                                                                          ttCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATc                                  >  2:3501513/1‑140 (MQ=255)
                                                                                                          ttCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATc                                  >  1:1181052/1‑140 (MQ=255)
                                                                                                          ttCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCATTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTCTTCATAAAAACGACTCGCCAACCGTACGGATCCTCTATc                                  >  2:3890822/1‑140 (MQ=255)
                                                                                                            ctttttACAAGGTTTTTCACATCGAATTGTCCTAAAATCCCCGTTAAACTTTCACCCTTCACCCCCAAAAACTTTACGGTGCATGATATCAGTTTTTTTTTAATAACAACGACTCGCCAACCGTCCGGATCCTCTATCGt                                <  1:1602224/138‑1 (MQ=255)
                                                                                                            ctttttACAAGCGTTTTCTCATCGAATTGTCCTAAAAAGCTCGTCAAACTTTTAGCCTTTAGCACGAAAAAGTGTTACTTGCCTGATAGCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                                <  2:1318208/138‑1 (MQ=255)
                                                                                                            cGTTTTACAAGGGTTTTCTAATCGCTTTGTCCTAAAAAGCCCGTTAAATTTTTATCCTTTAGCACTAAAAATTGTTAGTTGCCTGATAGCCCTTTTGTTTACATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGt                                <  2:1675857/140‑1 (MQ=255)
                                                                                                            cGTTTTACAAGCTTTTTCTCATCGCATTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGt                                <  2:4144061/140‑1 (MQ=255)
                                                                                                              ttttACAAGCGTTTTCTCATCTCATTTTCATAAAAAGCTCGTTAAACTTTTATCTTTTAGAACGAAAAAGAGTTACTTGCCTTATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                              <  2:595104/140‑1 (MQ=255)
                                                                                                              ttttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTCTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                              >  2:1577310/1‑140 (MQ=255)
                                                                                                              ttttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                              >  2:343867/1‑140 (MQ=255)
                                                                                                              ttttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                              >  2:1937045/1‑140 (MQ=255)
                                                                                                              ttttACAAGCGTTTTCTCAGCGCTTTGTACTAAAAAGCTCGTCAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCGTTTTTTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                              <  2:1654090/140‑1 (MQ=255)
                                                                                                              ttttAAAAGGTTTTTCTCATCGCTTTGTCATAAAAAGCTCGTTAAAATTTTATCCTTTAGCACCAAAAATATTTAATTGCCTGATATCCCTTTTTTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                              <  2:155782/140‑1 (MQ=255)
                                                                                                              tttaACAAGGTTTTTATCATCGATTTGTACTAAAACGCTCGTTAAATTTTTATCTTTCACCACTAAAACTAGTTTGTTGCGTGATATCCTTTTTGTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                              <  1:1148893/140‑1 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                             >  1:2111098/1‑140 (MQ=255)
                                                                                                               tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                             >  1:3818583/1‑140 (MQ=255)
                                                                                                                ttAAAAGCGTTTTATACTCGCATTGTCCAAAAAAGCTCGTGAAACTTTTTTCGTTTCGCACTAAAAAGATTTAGTTGCCTGTTATCCCTTTTGTATTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                            <  2:3984526/140‑1 (MQ=255)
                                                                                                                 tACAAGGGTTTTATCTTGGCTTTGTCATAAAATGCTCGTTAAACTTTTATCCTTTACCACTAAAACTAGTTACTGGCCTGATATCCCTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                           <  2:1722725/140‑1 (MQ=255)
                                                                                                                 tACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGc                           >  2:3053008/1‑140 (MQ=255)
                                                                                                                      ggtttttCTCATCGCTTTGTCCAAAAAAGATCGTTAAAATTTTATCCTTTCGCACCAAAAAGAGTTAGTTGCCTGATATCCCTTTTTTTTTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                      <  1:352594/137‑1 (MQ=255)
                                                                                                                      gggTTTTCTAAGCGCTTTGTCCAAAAATCCTCGTCAAAGTTTTAACCTTTAGCACTAAAAAGAGTTCGTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                      <  1:4011623/138‑1 (MQ=255)
                                                                                                                      gctttttCTCATCGAATTGTCCTAAAAAGCTCGTGAAAATTTTATCCTTTAGCACGAAAAAGAGTTACTGGCCTGATATCCCTTTTTTATTCATATACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                      <  2:2895154/137‑1 (MQ=255)
                                                                                                                      gCGTTTTCTAATCGCATTGTCAAAAAAAGCTCGTTAAAATTTTATCCTTTAGCACGAAAAAGATTTACTGGCCTTATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                      <  2:1181052/140‑1 (MQ=255)
                                                                                                                       gtttttCTCTTCGTAGTGTAAAAAAAAGCTCCTTAATGTTTTATCCTTCACCACAAAATATAGTTTTTTGCATTATAGCCCTTTTTTATTCTTAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  1:2427168/138‑1 (MQ=255)
                                                                                                                       gtttttCTCATCGCATTGTCCTAAACAGCTCGTTAAAATTTTATCCTTTAGCACGAAAAAGATTTAGTTGCCTGATATCCATTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  2:1513199/138‑1 (MQ=255)
                                                                                                                       ggTTTTCTCATCGCTTTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTCCCACTAAAAAGAGTTAGTTGCCGGATATCCCTTTTGTTTCCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  2:4036074/139‑1 (MQ=255)
                                                                                                                       ggTTTTATCATCTCATTGTCATAAAAAGCTCGTGAAACTTTTATCCTTTACCACTAAAAAGAGTTACTTGCCTCATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  2:492080/139‑1 (MQ=255)
                                                                                                                       ctttttCTAATCGCATTGTCATAAAAACCTCTTTAAAATTTTATCCTTTAGCACTAAAAATAGTTACTTGCATGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  2:2971009/138‑1 (MQ=255)
                                                                                                                       cGTTTTCTCATCGAATTGTCCTAAAAAGCTCGTTAAAATTTTATCCTTTACCACTAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                     <  2:4159699/140‑1 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCCTAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                   >  2:4049896/1‑140 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                   >  1:4262120/1‑140 (MQ=255)
                                                                                                                         ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                   >  1:178553/1‑140 (MQ=255)
                                                                                                                          tttCTCTGCGCATTGGCCTAAAATGCGCTTCAAAGTTTTAGCCTTTCCCACGAAAAAGTGTTCGGTGCCTGATATCCCTTGTTTTTTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                  <  1:1290698/140‑1 (MQ=255)
                                                                                                                          tttCTCATCGAATTGTCCAAAAAAGCTCGTGAAAATTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                  <  2:3304570/140‑1 (MQ=255)
                                                                                                                          tttATCATCGCTTTGTCCAAAAACGCTCTTTAAAATTTTATCCTTTACCACCAAAAATAGTTACTTTCATGATAGCCTTTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                  <  1:789907/140‑1 (MQ=255)
                                                                                                                                 tCGCATTGGCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTCTCGTCACGCCTCCATGGCTCCAGTa           >  2:751799/1‑140 (MQ=255)
                                                                                                                                      ttGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGt      >  1:1314826/1‑140 (MQ=255)
                                                                                                                                         tCCTAAACAGCTCGTTAAACTTTTATCCTTTAGCACTAAAATGAGTTACTTGCCTGATATCCCTTTTGTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct   <  1:2929143/140‑1 (MQ=255)
                                                                                                                                         tCCTAAAAAGCTCGTCAAACTTTTACCCTTTCCCACCAAAAAGTGTTAGTTGCATGATATCCCTTTTGTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct   <  1:392101/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCAAAACGATTCTATTCAAACAAATCAAGTTTATCAGTGAAGAAACAGAAAGTCAATTGTAAATCCGAACATTAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCCTTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTC  >  NZ_CP034943/3996718‑3996995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: