Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 4,022,597 G→T 5.3% V607F (GTT→TTT)  EO946_RS20675 → thioredoxin domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349434,022,5970GT 5.3% 915.9 / 2.1 334V607F (GTT→TTT) EO946_RS20675thioredoxin domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (171/128);  new base T (3/32);  total (174/160)
Fisher's exact test for biased strand distribution p-value = 1.39e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 339 total aligned reads displayed.

AGTCACAGGCGACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTA  >  NZ_CP034943/4022458‑4022736
                                                                                                                                           |                                                                                                                                           
 gTCACAGGCGACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGt                                                                                                                                            >  2:808320/1‑140 (MQ=255)
 gTCACAGGCGACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGt                                                                                                                                            >  1:3928893/1‑140 (MQ=255)
          gACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTgg                                                                                                                                   <  1:3799387/140‑1 (MQ=255)
           aCTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCACAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGc                                                                                                                                  >  1:558391/1‑140 (MQ=255)
           aCTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGc                                                                                                                                  >  1:350419/1‑140 (MQ=255)
           aCTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGc                                                                                                                                  >  1:210309/1‑140 (MQ=255)
           aCTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGc                                                                                                                                  >  1:3117541/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGTATATCGAATCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAATGAAATCGTTATTTTTGGCAGAGCAGACGa                                                                                                                       >  1:246095/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  2:2675077/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  1:3724049/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  1:252075/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTGTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  2:1356240/1‑140 (MQ=255)
                      aTTGAAAAAGCAGAAACGATGGTTTCTGTGTTCAAGCCGGATATCGACGCGTATCCGAGCGGCCATGCGTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCTTTATTTTTGGCAGCGCAGACGa                                                                                                                       >  2:571066/1‑140 (MQ=255)
                         gAAAAAGCAGAAACGATGTTTTCTATGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATcc                                                                                                                    <  1:3605739/140‑1 (MQ=255)
                            aaaGCAGCAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGAGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATccagc                                                                                                                 <  2:3605314/140‑1 (MQ=255)
                            aaaGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAAGCGGTATTTTTGGCAGCGCAGACGATccagc                                                                                                                 <  1:1150776/140‑1 (MQ=255)
                            aaaGCAGAAACGATGTTTTCTGTGTTCAAGACGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATccagc                                                                                                                 <  1:766363/140‑1 (MQ=255)
                                   aaaCGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaaa                                                                                                          <  1:2680193/140‑1 (MQ=255)
                                   aaaCGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaaa                                                                                                          <  1:1206335/140‑1 (MQ=255)
                                        aTGTTTTGTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTCTGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     <  1:2381108/140‑1 (MQ=255)
                                         tGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATc                                                                                                    >  2:4014794/1‑140 (MQ=255)
                                           ttttCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATcac                                                                                                  <  1:3428524/140‑1 (MQ=255)
                                                 gtgtTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGc                                                                                            >  1:1450519/1‑140 (MQ=255)
                                                  tgtTCAAGCCGGATATCTAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTTATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCa                                                                                           <  1:1309625/140‑1 (MQ=255)
                                                  tgtTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCa                                                                                           <  1:4184358/140‑1 (MQ=255)
                                                       aaGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCa                                                                                      >  2:1396348/1‑140 (MQ=255)
                                                       aaGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCa                                                                                      >  2:3284144/1‑140 (MQ=255)
                                                       aaGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCa                                                                                      >  2:2400046/1‑140 (MQ=255)
                                                       aaGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACCGCACTTCa                                                                                      >  2:1958748/1‑140 (MQ=255)
                                                          ccGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCaaaa                                                                                   <  2:526904/140‑1 (MQ=255)
                                                              atatCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGcc                                                                               >  2:2224450/1‑140 (MQ=255)
                                                                  cGAATCGTATCCGATCGGCGATTCTTTCTGTATGCAAATCGTTTTAAAGCATTTTATGCCTAAAAATGAAATCGTTATTTTTTGCAGCGCAGACGATCCAGGCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTa                                                                           <  1:808320/140‑1 (MQ=255)
                                                                  cGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGaaaa                                                                                                          >  2:2736179/1‑109 (MQ=255)
                                                                  cGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTa                                                                           <  2:2898371/140‑1 (MQ=255)
                                                                  cGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCACCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTa                                                                           <  1:2805957/140‑1 (MQ=255)
                                                                         tATCCGATCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCaa                                                                    >  2:3011569/1‑140 (MQ=255)
                                                                         tATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCaa                                                                    >  2:3069237/1‑140 (MQ=255)
                                                                                gCGGCCATGCTTTCTTTATGAAAAGCGTTTTAAAGAATGTTATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCCGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTcc                                                             <  2:671448/140‑1 (MQ=255)
                                                                                   gCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAATCAAATCACCACTGCACTGCAAAAAGCCTTTAAGCCCAACGACTCCAtt                                                          >  1:948055/1‑140 (MQ=255)
                                                                                    ccATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTa                                                                           >  2:3395374/1‑122 (MQ=255)
                                                                                     cATGCTTTCTTTATGCAAAGCTTTTTAAAGCATGTCATCCCAAAAAACCAAATCGTTATTTTTGGCAGCGCAGACGACCCACCCACAAAAAAAATCACCACTCCACTAAAAAAACCCTTTAACCCCCACGACTCCAtttt                                                        <  1:1569878/140‑1 (MQ=255)
                                                                                       tGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAt                                                                                                     >  2:1308045/1‑93 (MQ=255)
                                                                                         gtttgtttATGAAAAGCGTGTTAAAGAATGTGATGCCTAAAACGGAAATCGTTATTTTTGGCAGCGCAGACGATCCACCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTtggtg                                                    <  2:2207431/139‑1 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAACGGAAATCTTTATTTTTGGCAGCGCAGCCGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAACCCCTTTAAGCCCCACGACTCCATTTTGGTGGCaga                                               <  2:606536/140‑1 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAATCCCAACGACTCCATTTTGGTGGCaga                                               >  1:4030279/1‑140 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               >  2:1045235/1‑140 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               >  2:3199864/1‑140 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               >  2:3769535/1‑140 (MQ=255)
                                                                                              tttATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTATGGTGGCaga                                               >  2:1677929/1‑140 (MQ=255)
                                                                                              ttgaTGCAAAGCGTGTTAAAGCATGTGATGCCTCAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCGAGAACACAAATCACCACTGCAGTGCAAGCCGCCGTTATGCCCAGCGGCTCACGTTTGGTGGgaga                                               >  2:3480608/4‑140 (MQ=255)
                                                                                              gtgaggCAACGCGTGTAAAAGCCTGTGAGGCACAAACAGGAAATCGTTTTTTTTGGCCGCGAAGTGGCGCCAGACACAAAACAAATCACCACGGCACTTCAAAAAGCCTTTAAGCCCAACGACGCCATTTTGGTGGCaga                                               <  2:3160109/135‑1 (MQ=255)
                                                                                                 aTGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCa                                            >  1:1767741/1‑140 (MQ=255)
                                                                                                  tGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCAt                                           >  1:1520359/1‑140 (MQ=255)
                                                                                                  tGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCAt                                           >  1:2673565/1‑140 (MQ=255)
                                                                                                   tcAAAGCGTGTTAAAGAATTTTATTCAAAAAAATTAAATCTTTATTTTTGGCAGCGCAGAAGATCCAGCCAGAAAAAAAATCACCACTGCAATTCAAAAAGCCTTTATGCCAATATAATACATTTTGGTGGCatagattc                                          <  2:2873882/139‑5 (MQ=255)
                                                                                                   gCAAAGGTTGTAAAAGCATTTTATGCCTAAAAAGGAAATGGTTATTTTTGGCAGCGCAGACTATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATc                                          <  2:2605356/140‑1 (MQ=255)
                                                                                                   gCAAAGCGTTTTAAAGCATGTTATGCCTAAAAAGCAAATCTTTATTTTTGGCAGCGCACACGATCCCGCCAGAAAACAAATCACCACTGCACTTAAAAAAGCATTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATc                                          <  1:670661/140‑1 (MQ=255)
                                                                                                      aaGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTg                                       >  1:4078753/1‑140 (MQ=255)
                                                                                                      aaGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTg                                       >  1:3033128/1‑140 (MQ=255)
                                                                                                      aaGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCATTTAAGCCCACCGACTCCAGTTTGGTGGCAGAGCATCCTg                                       >  1:1875872/1‑140 (MQ=255)
                                                                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                      >  2:1632757/1‑140 (MQ=255)
                                                                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                      >  1:485029/1‑140 (MQ=255)
                                                                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                      >  1:3224413/1‑140 (MQ=255)
                                                                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                      >  1:2277794/1‑140 (MQ=255)
                                                                                                       aGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGa                                      >  1:2648906/1‑140 (MQ=255)
                                                                                                        gCGTTTTAAAGCTTGTGATGCCTAAAAAGTAAATCGTTTTTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGAt                                     <  1:2809776/140‑1 (MQ=255)
                                                                                                        gCGTGTTAACGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCAACAAAAAAAAACACCACTGCACTTAAAAAAGCCTTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGAt                                     <  1:3871610/140‑1 (MQ=255)
                                                                                                        gCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCTTTATTTTTGGCAGCGCACACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCGTTAAGCCCAACGACTCCATTTTGTTGGCAGAGCATCCTGAt                                     >  1:2299930/1‑140 (MQ=255)
                                                                                                        aggtgtTAAAGCATGTTATGCCTAAAAAGGAAATCGTTATTTTTGGAAGCGCATACGATCCAGCCATAAAACAAATCACCACTGCCCTTCAAAAAGAATTAAAGCCCTTCGACTAAATTTTGGTGGCATAGCATCGTGAt                                     <  2:1221116/138‑1 (MQ=255)
                                                                                                         cGTGTTAACTCTTGTGATGCATAAAAAGGAAATGGTTATTTTTGGAAGCGCAGACTATCCAGCCAGAAAACAAATCACCACTCCACTTCAAAAAGCCTTTATGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATc                                    <  2:4262021/140‑1 (MQ=255)
                                                                                                            gtTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTg                                                                                             <  2:2543457/80‑1 (MQ=255)
                                                                                                            gtTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAAt                                 >  2:2156181/1‑140 (MQ=255)
                                                                                                            gtTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAAt                                 >  2:1183263/1‑140 (MQ=255)
                                                                                                               aaaGAATGTTATTCATAAAAAGTAAATATTTATTTTTGTCAGCGCATACGATCCAGCCAGAAAACAAATCACCACTGAACTTAAAAAATCTTTTAAGACAAACGACTGCATTTTTTTGTCATAGCATAGTGATCGAttct                              <  2:3268736/140‑4 (MQ=255)
                                                                                                                aaGAATGTGATGCCTAAAAAGGAAATGGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACCAGGGCACTTCAAAAACCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                             <  1:1275095/140‑1 (MQ=255)
                                                                                                                aaGAATGTGATGCATAAAAAGTAAATCTTTATTTTTGGCAGCGCATACGATCCAGCAAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCaa                             <  1:3325550/140‑1 (MQ=255)
                                                                                                                  gCATGTGATGCCTAAAAAGCAAATCGTTATTTTTGGCAGCCCACACTATCCAGCCACAAAAAAAATCACCACTCAACTTCAAAAAGCATTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAg                           <  1:1959109/140‑1 (MQ=255)
                                                                                                                   cATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCaga                                               >  1:3585931/1‑119 (MQ=255)
                                                                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCTGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAGACAGCCTGTACGCCCACCGACTCCTTGTTGGGGGCTGAGCATCGTGATCAATGCAAAGACa                        >  2:4202091/1‑140 (MQ=255)
                                                                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACa                        >  2:247433/1‑140 (MQ=255)
                                                                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACa                        >  1:2897108/1‑140 (MQ=255)
                                                                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACa                        >  1:2010036/1‑140 (MQ=255)
                                                                                                                     tgtgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACa                        >  2:2313064/1‑140 (MQ=255)
                                                                                                                       tgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAACAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCa                                                 <  2:4147208/113‑1 (MQ=255)
                                                                                                                       tgATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCa                                                 <  2:730213/113‑1 (MQ=255)
                                                                                                                         aggCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCCGCCAGAAAACAAATCACCACTGCACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGc                    <  2:2920651/138‑1 (MQ=255)
                                                                                                                         aTGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGc                    <  2:1250817/140‑1 (MQ=255)
                                                                                                                         aTGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGc                    <  2:3873535/140‑1 (MQ=255)
                                                                                                                          tGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCATAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCa                   <  2:409871/140‑1 (MQ=255)
                                                                                                                            ccTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCAcc                 >  1:897344/1‑140 (MQ=255)
                                                                                                                              tAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCATAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCt               <  2:156820/140‑1 (MQ=255)
                                                                                                                                 aaaGGAAATCTTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATCACCCCTGCACTTCAAAAAGCATTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTg            <  1:4149745/140‑1 (MQ=255)
                                                                                                                                    ggAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcag         <  2:3053570/140‑1 (MQ=255)
                                                                                                                                    ggAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcag         <  2:2054785/140‑1 (MQ=255)
                                                                                                                                    ggAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGAACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcag         <  2:3651528/140‑1 (MQ=255)
                                                                                                                                    ccaaaTCGTTATTTTTGGCAGCGCCTACGATCCCTCAAGAAAAAAACTAACCACGGCACTTCAAAAAGCCTTTAACCCCAACGAATCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcag         <  2:3742160/138‑1 (MQ=255)
                                                                                                                                      acaTCGTTTTTTTTGGCAGCGCTGACGTGCCCGCCAGAACACAAATCACCACTGCACTTCAAAAACCCTTTACGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTgcagca       <  1:3088173/138‑1 (MQ=255)
                                                                                                                                       aaTCTTTTTTTTTGGCAGCGCAGACGATCCAGCCAGAAAAAAAATAACCACTGCACTTCAAAACGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGcagcag      <  1:4220325/140‑1 (MQ=255)
                                                                                                                                        aTCGTAATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAAACACCACTGCACGTCAAAAAGCCTTAAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTATGCAGCAGa     >  2:1860902/1‑140 (MQ=255)
                                                                                                                                         tCGTTATTTTTGGCAGCGCAGACTATCCACCCAGAAAACAAATCACCACTGCACTTCAAAACGCCTTTAACCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGAc    <  1:1027636/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGTCACAGGCGACTTATCACTCATTGAAAAAGCAGAAACGATGTTTTCTGTGTTCAAGCCGGATATCGAAGCGTATCCGAGCGGCCATGCTTTCTTTATGCAAAGCGTGTTAAAGCATGTGATGCCTAAAAAGGAAATCGTTATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATCACCACTGCACTTCAAAAAGCCTTTAAGCCCAACGACTCCATTTTGGTGGCAGAGCATCCTGATCAATGCAAAGACATAGCACCCTTTGCAGCAGACTA  >  NZ_CP034943/4022458‑4022736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: