Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 376,966 A→C 5.2% K781T (AAA→ACA)  srfAA → surfactin non‑ribosomal peptide synthetase SrfAA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943376,9660AC 5.2% 824.7 / 6.2 348K781T (AAA→ACA) srfAAsurfactin non‑ribosomal peptide synthetase SrfAA
Reads supporting (aligned to +/- strand):  ref base A (147/167);  new base C (32/2);  total (179/169)
Fisher's exact test for biased strand distribution p-value = 2.54e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

TTGAAGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACGGCTGAGA  >  NZ_CP034943/376830‑377103
                                                                                                                                        |                                                                                                                                         
  gAAGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAgcgc                                                                                                                                      <  2:4049937/140‑1 (MQ=255)
    aGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCTGATAGTTACGTTCAATCAGCGTATGTGACGATTGTGaatgtgttg                                                                                                                                    <  1:2662409/140‑8 (MQ=255)
    aGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTg                                                                                                                                    <  1:899337/140‑1 (MQ=255)
        aTTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGa                                                                                                                                >  2:1275646/1‑140 (MQ=255)
          ttAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGCCCGCGCTGGGGAAt                                                                                                                              >  2:1152079/1‑140 (MQ=255)
          ttAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAAt                                                                                                                              >  2:490241/1‑140 (MQ=255)
           tacagaagCAGGCACAGGGCCGCGTTTGACAAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCTTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGAGTATGTGCCGATCGGGAAAGCGCTGGGGAATa                                                                                                                             <  1:2174538/137‑1 (MQ=255)
            aaaGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATaa                                                                                                                            <  2:3005868/140‑1 (MQ=255)
            aaaGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATaa                                                                                                                            <  2:3596251/140‑1 (MQ=255)
                 agCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGCAAGCGCTGGGGCATACCCGtt                                                                                                                       >  2:1244943/1‑140 (MQ=255)
                   cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAACCGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTta                                                                                                                     >  2:856578/1‑140 (MQ=255)
                   cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTta                                                                                                                     >  1:1099965/1‑140 (MQ=255)
                   cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTg                                                                                                                                    >  2:3903118/1‑125 (MQ=255)
                   cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTta                                                                                                                     >  2:939961/1‑140 (MQ=255)
                   cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTta                                                                                                                     >  2:137429/1‑140 (MQ=255)
                   cAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTta                                                                                                                     >  1:3170177/1‑140 (MQ=255)
                     ggCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTtata                                                                                                                   <  2:538477/140‑1 (MQ=255)
                            cgccgcGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTg                                                                                                            >  1:2893583/1‑140 (MQ=255)
                            cgccgcGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTg                                                                                                            >  1:1227253/1‑140 (MQ=255)
                                 cgTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCa                                                                                                       <  1:4242072/140‑1 (MQ=255)
                                            tgtgTACGGGCCTACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCTTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTTGGGAATAACCGTGTATATATTTTGGATCAAAGaggcaggc                                                                                            >  1:377247/1‑140 (MQ=255)
                                            tgtgTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGaggcaggc                                                                                            >  1:3713589/1‑140 (MQ=255)
                                              tgtACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTg                                                                                          <  1:461656/140‑1 (MQ=255)
                                              tgtACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGGGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTg                                                                                          <  2:397306/140‑1 (MQ=255)
                                                   gggCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGcc                                                                                     <  2:33050/140‑1 (MQ=255)
                                                     gCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAACGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                   >  2:4242016/1‑140 (MQ=255)
                                                      ccGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGa                                                                                  >  1:1711394/1‑140 (MQ=255)
                                                        gACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAg                                                                                >  1:248956/1‑140 (MQ=255)
                                                        gACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAg                                                                                >  1:247099/1‑140 (MQ=255)
                                                          cTGAAACGTGTGTTGACGCGTCCGTTCCTCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCGATGAGGCAGGCTGCAGCCGGAAGgt                                                                              >  2:809425/1‑140 (MQ=255)
                                                                   gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                     <  2:1898896/140‑1 (MQ=255)
                                                                   gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                     <  1:1244943/140‑1 (MQ=255)
                                                                   gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                     <  2:2647297/140‑1 (MQ=255)
                                                                   gtgtTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                     <  1:1410359/140‑1 (MQ=255)
                                                                      ttGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAg                                                                  <  2:247099/140‑1 (MQ=255)
                                                                             gTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTtata                                                           >  1:1631689/1‑140 (MQ=255)
                                                                               ccGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATc                                                         <  1:2278433/140‑1 (MQ=255)
                                                                                cGTTCATCTGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCg                                                        <  2:3861807/140‑1 (MQ=255)
                                                                                cGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCg                                                        <  2:3494556/140‑1 (MQ=255)
                                                                                agTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCg                                                        <  2:140783/139‑1 (MQ=255)
                                                                                  ttCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCa                                                      <  1:3881847/140‑1 (MQ=255)
                                                                                  ttCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCa                                                      <  1:2191793/140‑1 (MQ=255)
                                                                                     atcaGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGc                                                   >  2:2389599/1‑140 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  2:1761326/140‑1 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  2:3400965/140‑1 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  2:1318905/140‑1 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  1:4035189/140‑1 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  1:2032731/140‑1 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  1:530231/140‑1 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  1:4108467/140‑1 (MQ=255)
                                                                                       caGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  2:3765570/140‑1 (MQ=255)
                                                                                         ggTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 <  1:3060666/138‑1 (MQ=255)
                                                                                         ggTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGa                                                 >  2:3060666/1‑138 (MQ=255)
                                                                                               cccAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTg                                         >  2:2496071/1‑140 (MQ=255)
                                                                                                   gagagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTCTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCg                                     <  2:4026984/140‑1 (MQ=255)
                                                                                                    agagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                   <  2:3642762/93‑1 (MQ=255)
                                                                                                    agagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                   <  2:403501/93‑1 (MQ=255)
                                                                                                    agagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                   >  1:3642251/1‑93 (MQ=255)
                                                                                                    agagTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGa                                    >  2:1683883/1‑140 (MQ=255)
                                                                                                      agTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                  <  1:2589869/140‑1 (MQ=255)
                                                                                                      agTGCCGTTCAATCAGAGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCTTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCTGGCGACGGTGTTGGCCGAgg                                  <  2:2248314/140‑1 (MQ=255)
                                                                                                      agTGCCGGTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                  <  1:879705/140‑1 (MQ=255)
                                                                                                       gTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGc                                 <  1:1019990/140‑1 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTCTGTGCCGATCGGGAAAGCGCTGGGGCATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  1:3450007/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGACCGCGCTGGGGCATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCCGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  2:3161816/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGGGTTGGCCGAGGCt                                >  2:211271/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAACGCGCTGGGGAATACCCGTTGATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTCTATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  2:1035536/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTggcgggc                                                                     <  1:3544483/103‑1 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAg                                                                  <  2:892092/106‑1 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                  >  2:598998/1‑138 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                  >  2:955736/1‑138 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                  >  2:2282731/1‑138 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                  <  1:598998/138‑1 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAgg                                  <  1:955736/138‑1 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  2:1800152/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  1:742537/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  2:1045056/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTGTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTAATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  2:4132013/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGGTTAGATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  2:3236600/1‑140 (MQ=255)
                                                                                                        tGCCGTTCAATCAGCGGATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTGATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCt                                >  2:2087272/1‑140 (MQ=255)
                                                                                                          ccGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTAt                              <  1:3955840/140‑1 (MQ=255)
                                                                                                          ccGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCGGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTAt                              <  1:3612509/140‑1 (MQ=255)
                                                                                                                  tCAGCGTATGTGCCGATCGGGACAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTCTTTACAttt                      >  2:2338534/1‑140 (MQ=255)
                                                                                                                  tCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCgg                                                                                   <  1:104501/79‑1 (MQ=255)
                                                                                                                          tgtgCCGATCGGGAAAGCGCTGGGGACTAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAAt              >  1:1963578/1‑140 (MQ=255)
                                                                                                                          tgtgCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAAt              >  2:2848836/1‑140 (MQ=255)
                                                                                                                          tgtgCCGATCGGGAAAGCGCTGGGGAATAACCGTATATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAAt              >  2:3726632/1‑140 (MQ=255)
                                                                                                                           gtgCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTGTATATCTCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAAtt             >  2:195765/1‑140 (MQ=255)
                                                                                                                            tgCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTg            >  1:2086830/1‑140 (MQ=255)
                                                                                                                             gCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGa           <  1:648410/140‑1 (MQ=255)
                                                                                                                                gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg        >  2:1573396/1‑140 (MQ=255)
                                                                                                                                gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg        >  2:2783724/1‑140 (MQ=255)
                                                                                                                                gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg        >  2:2895179/1‑140 (MQ=255)
                                                                                                                                gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg        >  1:320433/1‑140 (MQ=255)
                                                                                                                                gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg        >  1:1929232/1‑140 (MQ=255)
                                                                                                                                gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg        >  1:1747708/1‑140 (MQ=255)
                                                                                                                                gATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACgg        >  1:1326889/1‑140 (MQ=255)
                                                                                                                                 aTCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGAGGGCGAGCTTTATATCTCAGGCGACGGTGTTGGCCGAGGCTATTTAAATTTGCCTGAATTGACGGc       >  2:2566828/1‑140 (MQ=255)
                                                                                                                                      gAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACGGCTgaga  <  1:2308515/140‑1 (MQ=255)
                                                                                                                                      gAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACGGCTgaga  >  1:182945/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TTGAAGCTATTTAAAGAAGCAGGCACAGCGCCGCGTTTGACCAATGTGTACGGGCCGACTGAAACGTGTGTTGACGCGTCCGTTCATCAGGTTATCCCAGAGAGTGCCGTTCAATCAGCGTATGTGCCGATCGGGAAAGCGCTGGGGAATAACCGTTTATATATTTTGGATCAAAGAGGCAGGCTGCAGCCGGAAGGTGTGGCGGGCGAGCTTTATATCGCAGGCGACGGTGTTGGCCGAGGCTATTTACATTTGCCTGAATTGACGGCTGAGA  >  NZ_CP034943/376830‑377103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: