Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 452,125 T→A 10.8% K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943452,1250TA10.8% 994.6 / 82.4 320K146I (AAA→ATA) EO946_RS02320dienelactone hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (135/149);  new base A (13/22);  total (149/171)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 327 total aligned reads displayed.

TTTTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATAC  >  NZ_CP034943/451986‑452263
                                                                                                                                           |                                                                                                                                          
  tttCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAAACGACACGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTTGGATGCTGTTGTTCCCTCAGtttt                                                                                                                                          >  1:1541945/1‑140 (MQ=255)
             tttttGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAAc                                                                                                                               >  2:1098669/1‑140 (MQ=255)
               tttGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCg                                                                                                                             <  2:3648105/140‑1 (MQ=255)
                  gatgatGGTAAGCGTTTTGAAGATTAGGGGCCTGTCAAAATAAACCGACTCGTGATTGGCAAAGCCTTTAAGAGAATCATATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCaa                                                                                                                          <  2:2804484/140‑1 (MQ=255)
                          aaaGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAac                                                                                                                  >  2:860752/1‑140 (MQ=255)
                          aaaGCGATTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAac                                                                                                                  <  2:3738768/140‑1 (MQ=255)
                            acggTTTTGACTAAGATGGGCCTTTCAAATTAAAGCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGGTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:1069180/137‑1 (MQ=255)
                            aGCTTTTTGCAGAAGAGTGGCCTGTCAAACTAAACCGACTCGGGATTGGAAAAGCAATTAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCATTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:2530681/140‑1 (MQ=255)
                            aGCTTTTTGAAGAAGAGTGTCCTGTCAAAATAAACCGACTCGGGATTGGCAAATCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:3098770/140‑1 (MQ=255)
                            aGCTTTTTGAAGAAGAGTGGCCTGTAAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGGTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:1729280/140‑1 (MQ=255)
                            aGCTTTTGGAAGAAGTGTGGCCTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGGTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  1:2116010/140‑1 (MQ=255)
                            aGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:1872256/140‑1 (MQ=255)
                            aGCGTTTTGAACAAGCGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:2893492/140‑1 (MQ=255)
                               gTTTTTAAGACGAGTGGCCTTTAAAAAAAAACCGACTCGGTATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATGGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACgg                                                                                                             <  2:1541945/140‑1 (MQ=255)
                               gTTTTGAAGAATCTTGGCCTTTAAAAATAAACCGACTCGTGATTGGCAAAGCCATGAAGAGAATCAAATTGATAAATCTCCAAATTGGGATGCTGTTTTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACgg                                                                                                             <  2:1217259/140‑1 (MQ=255)
                                             tGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGa                                                                                               >  2:346458/1‑140 (MQ=255)
                                             tGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGa                                                                                               >  2:676150/1‑140 (MQ=255)
                                               gcgtttaaaaaTAAACCGACTCGGTATTGGCAAAGCCATGAAGTGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCATTTTTTTGATAAGAACCGCACCAACAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:378257/133‑1 (MQ=255)
                                               gcatttaaaaaTAAACCGACTCGGGATTGGCAAAGCCATTAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:2395410/133‑1 (MQ=255)
                                               gCCTGTCAAAATAACCCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATGGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:33539/140‑1 (MQ=255)
                                               gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             >  2:1996883/1‑140 (MQ=255)
                                               gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             >  2:175129/1‑140 (MQ=255)
                                               gCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:1039352/140‑1 (MQ=255)
                                               gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCCGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  2:78528/140‑1 (MQ=255)
                                               gCCTGTAAAAATAAACCGACTCGGGATTGGCAAAGCAATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATAAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:996438/140‑1 (MQ=255)
                                               gCCTGTAAAAAAAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  2:3499888/140‑1 (MQ=255)
                                               gCCTGAAAAATTAAACCGACTGGGGATTGGCAAAGCCATGAAGAGCATCAATTTTATAAATCTCCTAATTGGGATGCTGTTGTTCCATCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:277193/140‑1 (MQ=255)
                                               cccTGTCAAAATCAACCGACTCGGGATTGGCAAAGCCATGCAGAGCATCAAATTGATAAATATCCACATTGGGATGCTGTTGTTCCCACAGTTTTTTGAAAAGAACCGCCACATCAAACGACGGTTCATATGAAGGGaaa                                                                                             <  1:2004313/139‑1 (MQ=255)
                                                  tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAATAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                          >  1:2973425/1‑140 (MQ=255)
                                                  tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                          >  1:3680870/1‑140 (MQ=255)
                                                  tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                          >  2:3699730/1‑140 (MQ=255)
                                                  tGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAAc                                                                                          >  1:1223428/1‑140 (MQ=255)
                                                    tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAATAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                        >  1:3617559/1‑140 (MQ=255)
                                                    tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                        >  1:3254323/1‑140 (MQ=255)
                                                    tCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACaa                                                                                        >  1:87796/1‑140 (MQ=255)
                                                         aTAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTTATAAATCTCCAAAGTGGGATGCTGTTGTTCCCTAAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGa                                                                                   <  2:3054307/140‑1 (MQ=255)
                                                              ccGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                              >  1:3827109/1‑140 (MQ=255)
                                                              ccGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                              <  1:1709104/140‑1 (MQ=255)
                                                              ccGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                              >  1:683168/1‑140 (MQ=255)
                                                              ccGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCggg                                                                              >  1:1655704/1‑140 (MQ=255)
                                                                  cTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGa                                                                          <  2:921463/140‑1 (MQ=255)
                                                                  cTCGGGATTGGCAAAGCCATGAACAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTATTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGa                                                                          <  2:4147645/140‑1 (MQ=255)
                                                                           ggCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                <  2:1965925/93‑1 (MQ=255)
                                                                           ggCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGACAAACAATAGGACCGGGCAGACTGGCACGt                                                                 >  2:623153/1‑140 (MQ=255)
                                                                            gCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                <  2:3254323/140‑1 (MQ=255)
                                                                            gCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTGTTTGATAAGAAGCGCAACATCAAACGACGGTTCATATGAAGGGAACAACAATAGGACCGGGCAGACTGGCACGtg                                                                >  2:2339485/1‑140 (MQ=255)
                                                                            gCAAAGCCATGAAGAGCATCAAATTGAAAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACTGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                <  1:92/140‑1 (MQ=255)
                                                                            gCAAAGCAATGCAGAGCATCAAATTGTTAAATCTCCAAATTGGGATGCTGTTTTTCCCTAACTTTTTTGATAACAACCGCAACAACAAACGACGGTTAATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                <  2:2827748/140‑1 (MQ=255)
                                                                            gCAAAGACATGAAGAGCATCAAATTGATAAATCTCCAATTTGGGATGCTGTTTTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                <  2:3138200/140‑1 (MQ=255)
                                                                                aGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACg                                                                  <  1:4110661/134‑1 (MQ=255)
                                                                                 gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATCAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATCTGACAGGGCAGACTGGCACGTGATGaa                                                           >  2:407713/1‑140 (MQ=255)
                                                                                 gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                >  2:427427/1‑135 (MQ=255)
                                                                                 gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGtg                                                                <  1:427427/135‑1 (MQ=255)
                                                                                 gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  2:1441807/1‑140 (MQ=255)
                                                                                 gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  2:533104/1‑140 (MQ=255)
                                                                                 gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  2:2916804/1‑140 (MQ=255)
                                                                                 gCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGaa                                                           >  2:1343787/1‑140 (MQ=255)
                                                                                     tGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAgaga                                                       <  1:1818209/140‑1 (MQ=255)
                                                                                      gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                      >  2:3613882/1‑140 (MQ=255)
                                                                                      gAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGaa                                                      >  1:2612536/1‑140 (MQ=255)
                                                                                         gagCATCAATTTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcg                                                   <  1:1157481/140‑1 (MQ=255)
                                                                                         gagCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGAAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcg                                                   <  1:2520717/140‑1 (MQ=255)
                                                                                           gCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                                 <  1:1962009/140‑1 (MQ=255)
                                                                                           gCATAAAATTGATAAATCTCAAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATcgcg                                                 <  1:2640508/140‑1 (MQ=255)
                                                                                            cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  2:2596822/1‑140 (MQ=255)
                                                                                            cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGt                                                >  1:4003926/1‑140 (MQ=255)
                                                                                            cATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTACCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGACGAGAATAGCGt                                                >  2:2268816/1‑140 (MQ=255)
                                                                                                   gtGATAAATCTCCAAAGTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCggg                                         <  2:3522773/139‑1 (MQ=255)
                                                                                                      aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAacga                                                                                                                >  2:2198406/1‑66 (MQ=255)
                                                                                                      aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGacc                                      >  2:3216906/1‑138 (MQ=255)
                                                                                                      aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAc                                      >  1:1089325/1‑140 (MQ=255)
                                                                                                      aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAc                                      >  2:3938100/1‑140 (MQ=255)
                                                                                                      aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAc                                      >  1:1471063/1‑140 (MQ=255)
                                                                                                      aTAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAAc                                      >  2:2707406/1‑140 (MQ=255)
                                                                                                      aTAAATCTCAAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGAAGGTTCATATGAAGGGAAAAAAAATAGGACCGGGCAGACTGGCACGAGATGAAGAGAATCTCGTATCCGGGAAc                                      >  1:2160363/1‑140 (MQ=255)
                                                                                                        aactctcCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAACCGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCg                                    <  2:3376387/137‑1 (MQ=255)
                                                                                                        aaaTCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCACACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCg                                    <  2:400167/140‑1 (MQ=255)
                                                                                                           tctcCCAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAAGCGCGTATCCGGGAACCGtaa                                 >  2:2113636/1‑140 (MQ=255)
                                                                                                           tctcCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGtaa                                 >  2:4111651/1‑140 (MQ=255)
                                                                                                             tcaaaaTTGGGATGCTGTTGTTCCATCAGTTTTTGGATAAGAACAGCAACATAAAACGACGGTTCATATGAAGTGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGtaata                               <  2:2160363/137‑1 (MQ=255)
                                                                                                                      ggATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGc                      >  1:2218813/1‑140 (MQ=255)
                                                                                                                         tGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTcc                   >  2:482745/1‑140 (MQ=255)
                                                                                                                           ctctagttCCCTCAGTTATTTGTTAAAAACACAAACATCAAACAACTGTTCATATGAAGGGAAAAAAAATAGGACAGGGAATAATGGAACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGc                 >  2:83536/6‑140 (MQ=255)
                                                                                                                           cTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGc                 >  1:3919882/1‑140 (MQ=255)
                                                                                                                            ttttgttCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  1:2717567/138‑1 (MQ=255)
                                                                                                                            tgttgtTCCCTCCCTATTTTGAAACGAACCGCAACATCAAACGACGCTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  2:2491305/140‑1 (MQ=255)
                                                                                                                            tgttgtTCCCTCATTTTTTTTATAAGAACCGAAAAATCAATCGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCTTAATTACAAACAGCTCCGCt                <  2:2257982/140‑1 (MQ=255)
                                                                                                                            tgttgtTCCCTCATTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  1:3536841/140‑1 (MQ=255)
                                                                                                                            tgttgtTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  1:860427/140‑1 (MQ=255)
                                                                                                                            tgttgtTCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCt                <  1:4190242/140‑1 (MQ=255)
                                                                                                                               tgttCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCAc             <  1:575407/140‑1 (MQ=255)
                                                                                                                                 ttCCCTCATTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCt           <  2:1788789/140‑1 (MQ=255)
                                                                                                                                  tCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTc          <  1:3104646/140‑1 (MQ=255)
                                                                                                                                  tCCCTCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTc          <  1:2850837/140‑1 (MQ=255)
                                                                                                                                      tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAATACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGACTCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc      >  1:800651/1‑140 (MQ=255)
                                                                                                                                      tCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc      >  2:1901650/1‑140 (MQ=255)
                                                                                                                                      tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc      >  2:3550131/1‑140 (MQ=255)
                                                                                                                                      tCAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGc      >  2:183322/1‑140 (MQ=255)
                                                                                                                                       cAGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCa     <  1:1312124/140‑1 (MQ=255)
                                                                                                                                        aGTATTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCAt    >  2:3000741/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTTTTCTGCCTCGTTTTTGATGATGGAAAGCGTTTTGAAGAAGAGTGGCCTGTCAAAATAAACCGACTCGGGATTGGCAAAGCCATGAAGAGCATCAAATTGATAAATCTCCAAATTGGGATGCTGTTGTTCCCTCAGTTTTTTGATAAGAACCGCAACATCAAACGACGGTTCATATGAAGGGAAAAACAATAGGACCGGGCAGACTGGCACGTGATGAAGAGAATCGCGTATCCGGGAACCGTAATAACAAACAGCTCCGCTCACCTCAGGCATAC  >  NZ_CP034943/451986‑452263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: