Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943595,2030CA28.4% 154.4 / 20.6 88T30K (ACG→AAG) EO946_RS03130MMPL family transporter
Reads supporting (aligned to +/- strand):  ref base C (20/43);  new base A (25/0);  total (45/43)
Fisher's exact test for biased strand distribution p-value = 6.26e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTGAA  >  NZ_CP034943/595068‑595338
                                                                                                                                       |                                                                                                                                       
atatTCACCATTCTAAAAAGCACATTTAAAGGCGGAGAGTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCCGCGGTTAGATTAGCAGTTACCGTCGTGTTGTGGTTGGTTTGTCCGAACTTATCgggg                                                                                                                                     >  1:1778006/1‑138 (MQ=255)
atatTCACCATTCTAAAAAGCACATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTGTCCGCACTTAACGGAg                                                                                                                                     >  1:1097871/1‑140 (MQ=255)
atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAg                                                                                                                                     <  2:1417900/140‑1 (MQ=255)
atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAg                                                                                                                                     <  2:895150/140‑1 (MQ=255)
atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAg                                                                                                                                     >  1:1417900/1‑140 (MQ=255)
atatTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTAGTTTTTTTTATACGAAATTAAAGGAg                                                                                                                                     >  1:1475395/1‑140 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTGTTCGAACTTAAAGGAGCt                                                                                                                                   >  2:1710769/1‑140 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTCCGAACTTAACCGAGCt                                                                                                                                   >  1:1596281/1‑140 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTACGAACATAAAGGAGCt                                                                                                                                   >  2:856256/1‑140 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTAGTTTTTTTTATCCGAACTTAACGGAGCt                                                                                                                                   >  2:1533395/1‑140 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTTTTTTATGCGAAATTAAAGGAGcg                                                                                                                                   >  2:760968/1‑139 (MQ=255)
  atTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTGTGGTTTGGTTTGTGCGAACTTATAGGAGCt                                                                                                                                   >  1:1106922/1‑140 (MQ=255)
     cACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                                                <  2:2074351/140‑1 (MQ=255)
     cACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGc                                                                                                                                <  1:2000673/140‑1 (MQ=255)
      aCCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGAAATTAAAGGAGCTTGcc                                                                                                                               >  2:943097/1‑140 (MQ=255)
        cATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACGTAAAGGAGCTGGccca                                                                                                                             >  1:968403/1‑138 (MQ=255)
          ttCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCaaac                                                                                                                           >  2:95380/1‑138 (MQ=255)
             tAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCaaa                                                                                                                        <  1:425136/140‑1 (MQ=255)
                  aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                   <  1:1291030/140‑1 (MQ=255)
                  aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                   <  1:1720722/140‑1 (MQ=255)
                  aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                   <  1:847799/140‑1 (MQ=255)
                  aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                   <  1:605813/140‑1 (MQ=255)
                  aGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAgggg                                                                                                                   <  1:131297/140‑1 (MQ=255)
                   gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                  <  1:161527/140‑1 (MQ=255)
                   gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                  <  2:133864/140‑1 (MQ=255)
                   gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                  <  2:704081/140‑1 (MQ=255)
                   gCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGc                                                                                                                  <  2:84713/140‑1 (MQ=255)
                    cAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTCCGAAATTAACGGAGCTGGCCAATCAAAAGGGGCa                                                                                                                 >  2:737062/1‑140 (MQ=255)
                        tttAAAGGAGGAGATTCTGCAGAATGAGAGCATTCATCAAATTCAAGTGGGAAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGcc                                                                                                             <  1:430026/140‑1 (MQ=255)
                        tttAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGcc                                                                                                             <  1:1164312/140‑1 (MQ=255)
                        tttAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGACAATCAAAAGGGGCAGGcc                                                                                                             <  2:782007/140‑1 (MQ=255)
                               gaggagATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTc                                                                                                      <  1:1623667/140‑1 (MQ=255)
                                      ttCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGa                                                                                               <  2:1711915/140‑1 (MQ=255)
                                        cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                             <  1:886873/140‑1 (MQ=255)
                                        cTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATg                                                                                             <  1:715014/140‑1 (MQ=255)
                                                tGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGGGTTTGTTTTATCCGAACTTAAAGGATCTGGCCAATCAAAAGGGGCAGGCCCACCTTCCTGCAGATGCTGTTTCa                                                                                     >  2:989269/1‑140 (MQ=255)
                                                   gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTTATTTTTTTTTAAACGAAATTAACAGATCTGGGCAATCAAAAGGGGCAAGCCCACCATCCAGCAAATGATGTTTCAGaa                                                                                  >  1:336315/1‑140 (MQ=255)
                                                   gagCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGAGTTTGTTTTGTCCCAAATTAAAAGATCTGGGCAATCAAAAGGGGCCGGACCAGCTTCAGGCGGATTCTTTTTAAGaa                                                                                  >  1:242690/1‑140 (MQ=255)
                                                     gCAATCATCAAATTCCAGTGGGCAATTGCAGCGGGTCTATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTGCGAACTTAAAGGACCGTGCCAATCAAAATGGGCAGGCCCACCGTGCGGCAGATGCTGTTTCAGaacg                                                                                >  2:661026/1‑138 (MQ=255)
                                                     gCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTTAGTTTGTTTTCTCCGAACTTAAAGGAGCTGGCCAAAAAAAAGGGGCAGGCCCAGCTTCCTGCACAATCTGTTTAACaaac                                                                                >  2:1672340/1‑139 (MQ=255)
                                                     gCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAag                                                                                >  2:1456108/1‑140 (MQ=255)
                                                              aaaTTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGc                                                                       <  1:152270/140‑1 (MQ=255)
                                                                         ggCAATTGCAGCGGTTATATTAGCACTTACCGTCGTGTTGTGTTTGTTTTTTACGAACGTAAAGGAGCGGGCCCATCAAAAAGGGGAGGCCCACCATCCAGCCCATGCTGTTTGAGAAAGAGCAAAATCCAAATTaaaaa                                                            >  1:52033/1‑139 (MQ=255)
                                                                            aaTTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAg                                                         <  1:253239/140‑1 (MQ=255)
                                                                                 cAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGga                                                    <  2:354489/140‑1 (MQ=255)
                                                                                    cGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGagaa                                                 <  1:1710769/140‑1 (MQ=255)
                                                                                      gTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTCTACGAACTTAACGGAGCTGGCCAATAAAAAGGGGCAGGCCCAGCTTCCTGCAGATTCTGTTTCAGAAACAGCAAATGCCATTTTAAAAAAAGCGGGagaaga                                               >  2:389778/1‑140 (MQ=255)
                                                                                      gTTATATTAGCACTTACCGTCGTTTTGAGTTTTTTTTATGCGAACTTAAAGGAGCTGGCCAAAAAAAAGGGGCAGGCCCACCTTCCGGCAGCTTGTGTTTTGGAAATAGAAAAAGCCAATTTAAAAAAAGCGGGcgaaga                                               >  1:1631871/1‑140 (MQ=255)
                                                                                             tAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAACAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                                        <  1:1267280/140‑1 (MQ=255)
                                                                                             tAGCACTTACCGTCGTTTTGAGTTTGTTGTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACaataat                                        <  2:826737/140‑1 (MQ=255)
                                                                                             tAGCACGTACCGTCGTTTTGCGTTTGTTTTCTCCGAACTTACCGGAGCTGGCCAAGCAAAAGGGCCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAAGGCCATTTTAAAACCAGCGGGAGAAGACaataat                                        <  2:1796628/140‑1 (MQ=255)
                                                                                              aGCACTTACCGTCGTTTTGAGTTTGTTTTATCCGAACGTAAACGAGCTGGCCCAACAAAACGGGCAGGCCCCCCCGACAGCCCCTGCTGGTTCAGAAAGAGCAAATGCCAATTTAAAACAAGCGGGCGAAGACaagactg                                       >  1:899845/1‑135 (MQ=255)
                                                                                              aGCACTTACCGTCGTGTTGGGTTTGTTTTGTGCGAACTTAACGGAGCTGGCCCATCAAAATGGGCCGGCCCACCGTCCGGCAGGAGCTGTTTCAGAAATAGCAAAAGCCCTTTTAAAAAAAACGGGTGAAGAAAAAAAtt                                       >  1:1478419/1‑140 (MQ=255)
                                                                                               gCACTTACCGTCGTGTTGAGTTTTTTTTGTCCCCACTTAACGGAGCTGGCCAAACAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCACAAAGAGCCAATGCCATTTTAAAAAAAGCGGGAGAAGACAATAAtta                                      >  2:562309/1‑139 (MQ=255)
                                                                                                cACTTACCGTCGTGTTGAGTTTGTTTTCTCCGAACTTAACCGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCg                                     >  2:1931809/1‑140 (MQ=255)
                                                                                                 aCTTACCGTCGTTTTGAGTTTTTTTTGTCCGAAATTAAAGGAGCTGGGCAATCAAAAGGGGCAGGCCCAGCGTGCTGCCGATGCTGTTTCAGAAAGATCAAATGCCATTTTAAAACAAACGGGAGAAGAAAATAATTAGa                                    >  2:50459/1‑140 (MQ=255)
                                                                                                 aCTTACCGTCGTTTTGAGTTTTTTTTCTCCGAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCACCTTACTGCAGATGCTGTTTAAGAAAGATCAAAAGCCAATTTAAAACAAGCGGGAGAAGACAAAAATTAGa                                    >  2:582555/1‑140 (MQ=255)
                                                                                                 aCTTACCGTCGTTTTGAGTTTGTTTTATCCGAACTTAAACGATCTGGGCAATCAAAATGGGCCCGCCCACCATACTGAAGATTCTGTTTCAAAAAGAACAAAAAGCAATTTAAAAAAACAGGGAGAACACAAAAAttaca                                    >  2:1292722/1‑137 (MQ=255)
                                                                                                 aCTTACCGTCGTTTTGAGTTTGTTTTATCCGAAATTAAAGGAGCTGGCCCAACAAAAGGGGCAGGCCCAGCTTCCTGCACATGCTGTTTCAGAAAGAGCAAAAGCCATTTTAAAAAAAACGGGAGAAGACAATAATTCGa                                    >  2:578607/1‑140 (MQ=255)
                                                                                                 aCTTACCGTCGTGTTGAGTTTGTTTTCTCCCAACTTAAAGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTACTGCAGAATCTTTTTCAGAAACAGCAAAAGCCCATTTAAAAAAAGCGGGAGAAGACaataaataga                                    >  2:1470478/1‑135 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGGGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                   <  2:1790843/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                   <  2:137576/140‑1 (MQ=255)
                                                                                                  cTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGAt                                   <  1:184569/140‑1 (MQ=255)
                                                                                                    tACCGTCGTTTTGTTTTTGTTTTCTCCGAACTTAAAGGAGCGGGGCAATAAAAAGGGGCCGGCCCAGCTTCCGGCCGATGCTGGTTAAGAAAGTGCACAAGCCATTTTAAAAAAAGCGGGAGAAAAACATAAATCGATCa                                 >  2:1390141/1‑140 (MQ=255)
                                                                                                     aCCGTCGTTTTGGGTTTTTTTTGTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCCGGCCCAGCTTCCGGCAGATTCTGTTTCAGAAAAAGCCAATGGCATTTTAAAACAAGCGGGAGAAGACAATAATTGGATCAg                                >  2:293100/1‑140 (MQ=255)
                                                                                                     aCCGTCGTTTTGAGTTTTTTTTGTCCGAACTTAAACGAGCTGGCCAATAAAAAGGGGCCGGCCCAGCTTACTGCAGATGCTGTTTAAGAAAGAGCAAATGCCATTTTAAAAAAAGCCGGAGAAGAAAATAATTCTATCaa                                >  2:1104286/1‑139 (MQ=255)
                                                                                                     aCCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCCATCAAAAGGGGCAGGCCCAGCTTCCGGCAGATGCTGTTTCAGAAAGAGCCAATGCTATTTTAAAACAAGCGGGCGAAGACAATAATTCGATCAg                                >  2:1831141/1‑140 (MQ=255)
                                                                                                     aCCGTCGTTTTGAGTTTGTTTTCTCCGAACATAAAGGAGCTGGCCAATAAAAAGGGGCAGGCCCAGCTTCCGGCAGATGCTGTTTCAGAAAGAGCCAATGCCATTTTAAAACAACCGGGAGAAGACAATAAATAGATCac                                >  2:763690/1‑139 (MQ=255)
                                                                                                     aCCGTCGTGTTGAGTTTTTTTTCTCCGAACTTAAAGGATCTGGGCCATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGATCCAATGCCATTTTAAAAAAAACGGGAGAAGACAATAATTCGATCAg                                >  2:1338316/1‑140 (MQ=255)
                                                                                                     aCCGTCGTGTTGAGTTTGTTTTCTCCGCACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGGTTCAGAAAGAGCAAATGGCATTTTAAAACAAGGGGGAGAAGACAATAATTCGATCAg                                >  2:1384788/1‑140 (MQ=255)
                                                                                                     aCCGTCGTGGTGAGTTTGTTTTCTCCGAACTTAACCGAGCGGGCCCATCAAAATGGGCCGGCCCAGCTGCCTGCCGATGCTGTTTCAGAAAGAGCAAATGCCAATTTAAAAAAACCGGGAGAAGACAAAAAATCGATCAg                                >  2:98024/1‑140 (MQ=255)
                                                                                                        gtcgtTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTAt                             <  1:1548946/140‑1 (MQ=255)
                                                                                                         tcgtTTTGTGTTTTTTTTGTCCGAACTTAAAGGGGCTGGGCAATCAAAAGGGGCAGGCCCAGCTTGCTTCAGATGGTGTTTCAGAAAGAGCCAAAGCCATTTTAAAAAAAACGGGAGAAGAAAATAATTAGATAAGTATc                            >  2:1726742/1‑140 (MQ=255)
                                                                                                             tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                        <  2:12941/140‑1 (MQ=255)
                                                                                                             tttGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCt                        <  2:1966979/140‑1 (MQ=255)
                                                                                                             gttGAGTTTGTTTTCTCCGAACATAACCGACCAGGCCAAAAAAAAAGGGCACGCCCCCCATACAGCAGAAGCTTTTTGATAAAGAGCAAATGCCATTTTAAAAAAAGAGGGAGAAGAAAAAAAATCGATCAGTATCGtat                        >  2:351008/2‑138 (MQ=255)
                                                                                                                  gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAACAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa                   <  1:2083455/140‑1 (MQ=255)
                                                                                                                  gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa                   <  2:1989491/140‑1 (MQ=255)
                                                                                                                  gtttgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACa                   <  1:943097/140‑1 (MQ=255)
                                                                                                                     tgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTa                >  1:271794/1‑140 (MQ=255)
                                                                                                                     tgtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTGTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGACGACAATAATTCGATCAGTATCGTCTTTACAttc                >  1:911788/1‑139 (MQ=255)
                                                                                                                      gtttTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAg               >  1:108019/1‑140 (MQ=255)
                                                                                                                         ttCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATc            <  2:968403/140‑1 (MQ=255)
                                                                                                                                   ttAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaa  <  1:1470478/140‑1 (MQ=255)
                                                                                                                                   ttAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaa  <  1:1292722/140‑1 (MQ=255)
                                                                                                                                   ttAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaa  <  1:50459/140‑1 (MQ=255)
                                                                                                                                   ttAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaa  <  1:578607/140‑1 (MQ=255)
                                                                                                                                   ttAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTgaa  <  1:582555/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
ATATTCACCATTCTAAAAAGCAAATTTAAAGGAGGAGATTCTGCAGAATGAGAGCAATCATCAAATTCAAGTGGGCAATTGCAGCGGTTATATTAGCACTTACCGTCGTTTTGAGTTTGTTTTCTCCGAACTTAACGGAGCTGGCCAATCAAAAGGGGCAGGCCCAGCTTCCTGCAGATGCTGTTTCAGAAAGAGCAAATGCCATTTTAAAACAAGCGGGAGAAGACAATAATTCGATCAGTATCGTCTTTACATTAGATCACGCTTTGAA  >  NZ_CP034943/595068‑595338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: