Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,730,0750GC27.4% 133.2 / 18.9 79E166Q (GAA→CAA) rodZcell shape determination protein RodZ
Reads supporting (aligned to +/- strand):  ref base G (32/21);  new base C (1/19);  total (34/45)
Fisher's exact test for biased strand distribution p-value = 1.24e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAC  >  NZ_CP034943/1729936‑1730206
                                                                                                                                            |                                                                                                                                   
cAGCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCACTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaag                                                                                                                                     >  2:1985257/1‑140 (MQ=255)
cAGCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTAAGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGATCTGAAAAAGAGTCAGACACTAAAAAAGAAACtaaag                                                                                                                                     >  2:1802166/1‑140 (MQ=255)
  gCGATGGTAACAATGCCGCATCTGAAAAATCCATTACGCACAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAATAGAAACgaaagac                                                                                                                                   >  1:1782141/1‑139 (MQ=255)
  gCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGTCCCTAAAACAGCAACgaaagaa                                                                                                                                   >  1:236881/1‑140 (MQ=255)
    gATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGaaagaaaa                                                                                                                                 >  1:1523699/1‑140 (MQ=255)
        gTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAGAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaaa                                                                                                                                  <  1:1067/135‑1 (MQ=255)
        gTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAGAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACgaaagaaa                                                                                                                                  >  2:1067/1‑135 (MQ=255)
               tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                      >  1:631812/1‑140 (MQ=255)
               tGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaa                                                                                                                      >  1:73548/1‑140 (MQ=255)
                gCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATaaaaa                                                                                                                     <  2:327441/140‑1 (MQ=255)
                  cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                   >  1:1475422/1‑140 (MQ=255)
                  cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                   >  2:1889045/1‑140 (MQ=255)
                  cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                   >  2:1888884/1‑140 (MQ=255)
                  cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                   >  1:2073521/1‑140 (MQ=255)
                  cGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaag                                                                                                                   >  2:801833/1‑140 (MQ=255)
                    cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                 >  1:1415356/1‑140 (MQ=255)
                    cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                 >  1:272110/1‑140 (MQ=255)
                    cATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAaagaa                                                                                                                 >  1:29547/1‑140 (MQ=255)
                          aaaaaTCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGc                                                                                                           >  2:1761693/1‑140 (MQ=255)
                            aaataaatTCCGAAAATTTAAAGCAAGTACGAAATCCAAAAAGATTCAACGATAAAAGAGAAACAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAATAAACGAAAGAAAAAGACGATAAAAAACAACAAAGCGa                                                                                                         <  2:1029435/140‑1 (MQ=255)
                            aaaTAATTTACTCAAATTTAATGAAATTACGAAATCCAAAAACTTTAAACTATAAAATATAATAAAAGAAA‑CAGCTCTTAAAAATTGGCAGACACTAAAAAAGAAACTAAAGAAAAAGAAGATAAAAAATAAGAAAGCGa                                                                                                         <  2:1868283/140‑1 (MQ=255)
                                         aaatttaaaGAAATTACCAAATCCAAAAATATTCAACGATAAAACACAATCAAAGAAA‑CAGCTCTCAAAAAGAGCCAGACACTAAAAAACAAACCAAATAAAAAGAAGATAAAAAAGAACAAAGCGACAGTGATAAACTg                                                                                            <  2:73548/134‑1 (MQ=255)
                                         aaaGTTAAAGAAAGAACGAAATCCCAAAAGATTCAACGCAAAAACATAATCAACGCAA‑CCCCTCTGAAAAAGAGGCAGACACTAAAACACAAACTAAAAAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTg                                                                                            <  2:631812/140‑1 (MQ=255)
                                         aaaGTGAACCCAAGTACGAAATCCAAAAAGATTCAACGATAAAAGAGAATCAACGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAACACGATAAAAAAGACGAACGCGACAGTGATAAACTg                                                                                            <  2:2073521/140‑1 (MQ=255)
                                         aaaGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAACAACGCGACAGTGATAAACTg                                                                                            <  2:1475422/140‑1 (MQ=255)
                                           aGTGAAAGCAAGTACTAAATACCAAAAGATTAAACGCTAAAAGATAATCAAAGCAA‑CAGCACTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGga                                                                                          <  2:1558997/140‑1 (MQ=255)
                                                aaGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGTTAAACTGGAGATTa                                                                                     >  1:1816448/1‑140 (MQ=255)
                                                aaGAAATTACGAAATCCCAAAAGATTCAACGCTAAAAGATAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTa                                                                                     <  2:1523699/140‑1 (MQ=255)
                                                     aGTACGACATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAATAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                >  1:1767632/1‑140 (MQ=255)
                                                     aGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGACACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCa                                                                                >  2:50988/1‑140 (MQ=255)
                                                      ttACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTATGAAAAAGAGGCACACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAACACCAAAGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                               <  2:1036547/139‑1 (MQ=255)
                                                      gaaCGAAATCCCAAATGATTCAACGCTAAACGATCATCAAAGCAA‑CACCTCTGAAAAAGAGGCAGACACAAAAAAAGAATCGAACGACAAATATTATAAACAACATTAAAGAGACAGTGATAAACTGGATATTAAAGCaa                                                                               <  2:1975504/138‑1 (MQ=255)
                                                      gTACTAAATCACAAAACTTTCAACGCTAAAAGAGAATAAAAGCAA‑CAGCTATGAAAAATAGGCAGACACTAAAAAATAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCaa                                                                               <  2:1506893/140‑1 (MQ=255)
                                                       tACTAAATCCAAAAAGATTCAACGCTAAAACATAATCAAAGCAA‑CAGATCTGAAAAAGAGGAAGACAATAAAAAATAAACCAAAGAAAAAGAATATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                              <  1:476440/140‑1 (MQ=255)
                                                       tACGAAATCCCAAACTATTAAACTCTAAACCAGAATCAAACCAA‑CAGCACTTAAAAAGAGCCAGACACTAAAAAAGAAACCAAACAAAAACAACATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAAc                                                                              <  2:342114/140‑1 (MQ=255)
                                                       tACGAAATCACAAACGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAACGAAAAAGAAGATAAAAAAGAAGAAAGCGTCAGTGATAAACTGGAGTTTAAAGCAAc                                                                              <  2:509574/140‑1 (MQ=255)
                                                       tACGAAAACACAACATATACAACCCTAAAACAGAATAAAAGCAC‑CCGCTCTTACAAAGAGTCAGACACTAACAAACAAACGACCCAAAACCAAGACACAAACCAAGAAAGCGCCAGTGATAAACTGGAGATTAAACCAAc                                                                              <  2:1675684/140‑1 (MQ=255)
                                                          taaaTCCAAAAAGATTCAACGATAAAAGAGAATAAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACAATAAAAAACAAACGAAATAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTgg                                                                           <  2:22391/139‑1 (MQ=255)
                                                            aaTCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCa                                                                         >  2:690286/1‑140 (MQ=255)
                                                              tCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACt                                                                       >  2:1461920/1‑140 (MQ=255)
                                                                 aaaaaTATTCAACGCTAAAAGATAATCAAAGAAT‑CCGCTATGAAAATGAGCCAGAAACTAAAAAATAAACTCAACAAAAACAAGATAAAAAAGCAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                    <  2:901993/139‑1 (MQ=255)
                                                                 aaaaaTATTCAACGATAAAACAAAATAAAAGAAA‑ACGCTCTTAAAAAGAGGCACACACTAAAAAACAAACCAAACAAAACCAACATAAACAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGaa                                                                    <  1:382289/139‑1 (MQ=255)
                                                                    aacataaaaCGCAAAAATACAA‑CAAAACAACCAGCTATCAAAAAGAGGCACAAAATAAAAAACAAACCAAACAAAAACACCAAAAAAACCAACACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAggg                                                                 <  1:1889045/133‑1 (MQ=255)
                                                                         tCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                            >  1:20335/1‑140 (MQ=255)
                                                                         tCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCAtt                                                            >  2:1702663/1‑140 (MQ=255)
                                                                            acgaaaaaaGATAAGAAAACCAA‑CAGCTCTCAAAAAGAGGAACACACTAAAAAAGAAACCAAACAAAAACAAGAAAAAAAAGAACAAACCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  2:1767632/135‑1 (MQ=255)
                                                                            acccTAAAACAGAAACAAACCAA‑AACCTCTTAAAAAGAGGCACAAAATAAAAAATAAACCAAAGAAAAAGAAGAAAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  2:20335/137‑1 (MQ=255)
                                                                            aCGCTAAAAGAGAATAAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  2:1917032/140‑1 (MQ=255)
                                                                            aCGCTAAAAGAGAAACAAAGCAA‑CAGCTCTGAAAAAGCGGAAGACAATAAAAAACAAACGAACCAAAAAGAAGAAAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  1:1013512/140‑1 (MQ=255)
                                                                            aCGCTAAAAGACCATCAAAGCAA‑CAGCTCTGAAAAAGAGGCACACACTAAAAAAGAAACGAAAGAAAAACAAGATAAAAAACAAGACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  2:2002717/140‑1 (MQ=255)
                                                                            aCGCTAAAACATAATCAAAGAAA‑CAGCTCTTAAAAAGAGGCAGACACTAAAAAAGAAACTAAATAAAAACAACATAAAAAAGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  2:29547/140‑1 (MQ=255)
                                                                            aCGATAAAATATAAAAAAAGCAA‑CAGCTCTGAAAACGAGGCAGACAATAAAAAACAAACCAAACAAAAAGACGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  2:272110/140‑1 (MQ=255)
                                                                            aCGATAAAAGAGAATAAACGCAA‑CAGCTCTTAAAAAGAGCCACACACTAAAAAAGAAACGAAAGAAAAACAAGATACAAACCAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAAc                                                         <  2:1415356/140‑1 (MQ=255)
                                                                                tAAAAGATCTTCAAATCAA‑CTGATCTTAAACAGAGGCCGACCCTAAAAAAGAAACTAAAGAAAACGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACt                                                     <  2:493150/140‑1 (MQ=255)
                                                                                tAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACt                                                     >  2:2061130/1‑140 (MQ=255)
                                                                                      cgactCAACGCAA‑CCCCTCTCAAAAAGAGCCAGACACTAAAAAAGAAACGCCACACAAACACCAAAAAAACGCAGACCGCGACAGTGATACACTGGAGATTAAAGCAACTGGCACGGAAGGGTCATTAACGACTTATGaa                                               <  2:1782141/136‑1 (MQ=255)
                                                                                      ataaTAAAAGCAA‑AAGCTCTTAAAAATAGGCATACACTAAAAAATAAACGAAAGAAAACGAAGATAAAAAAGATGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                               <  2:992748/138‑1 (MQ=255)
                                                                                      agaATCAAAGCAA‑CAGCTCTGAAAAAGAGCCAGACACTAAAAAACAAACGAAACAAAAACACCATAAAAACGAACAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                               <  2:464244/140‑1 (MQ=255)
                                                                                      acaaaaaaaGAAA‑CATCTCTTAAAAAGAGGCACACAATAAAAAAAAAACCAAAAAAAAACAACAAAAAAAACACCACAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                               <  1:624440/134‑1 (MQ=255)
                                                                                      acaaTAAAACCAA‑CAGATCTTAAAAAGAGCCACACAATAAAAAACACACCAAACAAAAACAACATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGaa                                               <  1:1677963/138‑1 (MQ=255)
                                                                                       caaTCAAAGCAA‑CAGATCTGAAAAAGAGTAACACACTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTCGAGATTAAAGCAACAGGAACTGAAGGGTCATTAACGACTTATGAAg                                              >  2:624440/2‑140 (MQ=255)
                                                                                        aaacAAAGCAA‑CAGCACTTAAAAATATGAAGACACTAAAAAAGAAACGAAAAAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAAAATAAAGCAAATGGAACTGAAGGGTCATTAACGACTTATAAAgt                                             >  2:611016/4‑140 (MQ=255)
                                                                                         aTCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAgtg                                            >  1:579270/1‑140 (MQ=255)
                                                                                                  a‑CAGCTCTGAAAAAGAGGCAGACACAAAAAAAGACACGAAAGAAAAAGAAGATAAAAATGAAGAAAGCGACAGTGATAAACTGGCGAGTAAAGCAACTGGCACTGAAGGGTCATTAACGAGGTATGAAGTGGGCGGCGCt                                   >  2:1355717/1‑140 (MQ=255)
                                                                                                    cAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAGAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTg                                  >  1:913341/1‑140 (MQ=255)
                                                                                                    cAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACGGTGATCAACTGGAGATTAAAGCACCTGGCACTGAAGGGTGATTAACGACGTATGAAGTGTCCGGCGCTg                                  >  1:2005260/1‑140 (MQ=255)
                                                                                                       ctctGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTgaga                               >  2:1102702/1‑140 (MQ=255)
                                                                                                            aaaaaGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTc                          >  1:1895812/1‑140 (MQ=255)
                                                                                                             aaaaGAGGCATAAACAAAAAAACAAACCAAACAAAAACACCATAAAAAACAACAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCg                         <  1:1096430/140‑1 (MQ=255)
                                                                                                             aaaaGAGCCATACACTAAAAAAGAAACCAAAGAAAAACAACATAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCg                         <  1:122587/140‑1 (MQ=255)
                                                                                                                 gagGCATACACTAAAAAATAAACTAAAGAAAAAGAAGATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                     <  2:917063/140‑1 (MQ=255)
                                                                                                                 gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                     >  2:418224/1‑140 (MQ=255)
                                                                                                                 gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                     >  1:615521/1‑140 (MQ=255)
                                                                                                                 gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                     >  1:1212550/1‑140 (MQ=255)
                                                                                                                 gagGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCt                     >  2:1963468/1‑140 (MQ=255)
                                                                                                                     cacacacTAAAAAAGAAACGAAACAAAAAGAAGATAAAAAACACCAACGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGaa                 <  1:1954192/137‑1 (MQ=255)
                                                                                                                      aGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAAc                >  1:1935604/1‑140 (MQ=255)
                                                                                                                         cacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa             <  2:579270/140‑1 (MQ=255)
                                                                                                                         aacTAAAAAAGACACCAACCAAACCCACGATAAAAAACCCGACCGCGACAGTGATAAACTGGAGATTAAAGCAACTGCCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa             <  1:1446508/139‑1 (MQ=255)
                                                                                                                         aacTAAAAAAGAAACGACAGAAAAAGAATATAAAAAACAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa             <  2:1408820/139‑1 (MQ=255)
                                                                                                                         aacTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGa             <  2:452089/139‑1 (MQ=255)
                                                                                                                                   gAACCGAAAGAAAAAGAAGATAAAAAAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa   <  2:995911/140‑1 (MQ=255)
                                                                                                                                   gAAACTAAAGAAAACGAAGATAAAACAGACGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTAATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa   <  2:995903/140‑1 (MQ=255)
                                                                                                                                   caaaCTAAACAAAAACAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGCCTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGa   <  2:181151/139‑1 (MQ=255)
                                                                                                                                    aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc  >  1:795349/1‑140 (MQ=255)
                                                                                                                                    aaaCGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAc  >  2:456453/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                   
CAGCGATGGTAACAATGCCGCATCTGAAAAATCAATTACGCAAAGTGAAAGCAAGTACGAAATCCCAAAAGATTCAACGCTAAAAGAGAATCAAAGCAA‑CAGCTCTGAAAAAGAGGCAGACACTAAAAAAGAAACGAAAGAAAAAGAAGATAAAAAAGAAGAAAGCGACAGTGATAAACTGGAGATTAAAGCAACTGGCACTGAAGGGTCATTAACGACTTATGAAGTGTCCGGCGCTGAGAAATTCGAGCTCGAACTGAAGGCATCAGAC  >  NZ_CP034943/1729936‑1730206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑