Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP034943 | 2,981,253 | 0 | C | T | 50.0% | ‑5.3 / 18.9 | 14 | noncoding (1267/1550 nt) | EO946_RS15435 | 16S ribosomal RNA |
Reads supporting (aligned to +/- strand): ref base C (0/7); new base T (1/6); total (1/13) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01 | |||||||||||
Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCC > NZ_CP034943/2981117‑2981370 | tggaCATGGCGCGGGAACGTATTCACCGATTCATGCTGATCCGCGATTACTAGCGATTGCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt < 2:1102335/137‑1 (MQ=1) tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCt < 2:665504/137‑1 (MQ=1) tggaCAAGGCCCGGGAACGTAGTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt < 1:1087460/137‑1 (MQ=1) gggaccaGGCCCGGGACCGTATTCACCGCCGCATGCTGATCCGCGAGTACTAGCGATTCCAGCTACACGCAGTCGCGTTGCAGACTGCGATCCCAACTGAGAACAGATTTGTGGGATTGGCGTAACCTCGCGGGTTCGCt < 1:218321/134‑1 (MQ=1) ggtaaCGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCt < 1:155665/137‑1 (MQ=1) cgtggCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCAc < 2:1522200/137‑1 (MQ=1) cgaggCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCAc < 1:733061/137‑1 (MQ=1) atgaCTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTCACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATa < 2:945636/137‑1 (MQ=1) atgaCTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCGGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATa < 2:55580/137‑1 (MQ=0) tcccGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATGTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGAc < 2:1347887/137‑1 (MQ=0) gaatGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTGTTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGAGTTGACGTCATCCCCACCTtcctc > 2:1460674/4‑140 (MQ=0) agcaCAGAGTTGTGGGATTGGCTTAACCTCGCGGTTGTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAg < 2:1882658/137‑1 (MQ=0) agaagtgATTTGTTGTATTGTCTTTTCCTCGCGGTTTTGCTGCACTTTGTTGTGTCCATTGTAGCACGTTTGTTGCCCAGGTCATTAGGGGCTTTATGATTTGAAGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAg < 2:1005939/134‑1 (MQ=1) gagtGGCTTAACCTCGCGGTGTCGCTGCCCTTTGGTCTGTCCATTGGAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACCTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGGGcc < 1:1759385/137‑1 (MQ=1) | TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCC > NZ_CP034943/2981117‑2981370 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |