Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,981,2530CT50.0% ‑5.3 / 18.9 14noncoding (1267/1550 nt)EO946_RS1543516S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/7);  new base T (1/6);  total (1/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCC  >  NZ_CP034943/2981117‑2981370
                                                                                                                                        |                                                                                                                     
tggaCATGGCGCGGGAACGTATTCACCGATTCATGCTGATCCGCGATTACTAGCGATTGCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt                                                                                                                    <  2:1102335/137‑1 (MQ=1)
tggaCAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCt                                                                                                                    <  2:665504/137‑1 (MQ=1)
tggaCAAGGCCCGGGAACGTAGTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCt                                                                                                                    <  1:1087460/137‑1 (MQ=1)
gggaccaGGCCCGGGACCGTATTCACCGCCGCATGCTGATCCGCGAGTACTAGCGATTCCAGCTACACGCAGTCGCGTTGCAGACTGCGATCCCAACTGAGAACAGATTTGTGGGATTGGCGTAACCTCGCGGGTTCGCt                                                                                                                    <  1:218321/134‑1 (MQ=1)
            ggtaaCGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCt                                                                                                        <  1:155665/137‑1 (MQ=1)
                          cgtggCATTCTGATCCACGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCAc                                                                                          <  2:1522200/137‑1 (MQ=1)
                          cgaggCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCAc                                                                                          <  1:733061/137‑1 (MQ=1)
                                             atgaCTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTCACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATa                                                                       <  2:945636/137‑1 (MQ=1)
                                             atgaCTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTTGCGGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATa                                                                       <  2:55580/137‑1 (MQ=0)
                                                                tcccGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATGTGTGGGATTGGCTTAACCTCGCGGTTTTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGAc                                                    <  2:1347887/137‑1 (MQ=0)
                                                                                  gaatGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTGTTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGAGTTGACGTCATCCCCACCTtcctc                                  >  2:1460674/4‑140 (MQ=0)
                                                                                                   agcaCAGAGTTGTGGGATTGGCTTAACCTCGCGGTTGTGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAg                 <  2:1882658/137‑1 (MQ=0)
                                                                                                   agaagtgATTTGTTGTATTGTCTTTTCCTCGCGGTTTTGCTGCACTTTGTTGTGTCCATTGTAGCACGTTTGTTGCCCAGGTCATTAGGGGCTTTATGATTTGAAGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAg                 <  2:1005939/134‑1 (MQ=1)
                                                                                                                  gagtGGCTTAACCTCGCGGTGTCGCTGCCCTTTGGTCTGTCCATTGGAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACCTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGGGcc  <  1:1759385/137‑1 (MQ=1)
                                                                                                                                        |                                                                                                                     
TGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCAGCTTCACGCAGTCGAGTTGCAGACTGCGATCCGAACTGAGAACAGATTTGTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCACCTTAGAGTGCC  >  NZ_CP034943/2981117‑2981370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: