| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP034943 | 3,245,050 | 0 | C | A | 36.8% | 46.5 / 58.3 | 57 | intergenic (‑72/+121) | EO946_RS16785/EO946_RS16790 | UvrD‑helicase domain‑containing protein/trimeric intracellular cation channel family protein |
| Reads supporting (aligned to +/- strand): ref base C (21/15); new base A (6/15); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTCCTGCAGCACCTCATCTATTCTTGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCTGTTAGTTATCTAAACGAAA > NZ_CP034943/3244915‑3245188 | gCTCCTGCAGCACCTCATCGACTCTTGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAata < 2:1172229/140‑1 (MQ=255) gcagcaCCTCATCTATTCTTGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGAt > 2:1202987/1‑140 (MQ=255) ccTCATCTATTCTTGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGc > 2:664718/1‑140 (MQ=255) aTCTATTCTTGACTGCTCGTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGc < 1:1304809/140‑1 (MQ=255) tcttCGTGACTGCTCTTCCTTACATTCCTTATCCTGCTGATTCATTAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACGGTAAAATATAATTGGATACAGATAATCAATATATGATTGAAGCTGGCaaa < 2:1074809/138‑1 (MQ=255) tATTCTTGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCaaa < 2:1477866/140‑1 (MQ=255) aTTCTTGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAAt > 2:1975834/1‑140 (MQ=255) tGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAAt < 2:796245/140‑1 (MQ=255) tGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAAt < 2:714640/140‑1 (MQ=255) gACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAAtt > 2:813752/1‑140 (MQ=255) gACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAAtt > 1:94421/1‑140 (MQ=255) gACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCaaa > 2:880011/1‑133 (MQ=255) gACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCaaa < 1:880011/133‑1 (MQ=255) gACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAAtt > 1:1652498/1‑140 (MQ=255) gACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAAtt > 1:2052927/1‑140 (MQ=255) ttctctTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTAt < 2:1515457/138‑1 (MQ=255) tGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATa < 1:411310/132‑1 (MQ=255) tGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATa > 2:411310/1‑132 (MQ=255) ttccttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAg < 1:1541158/140‑1 (MQ=255) ttccttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAg < 1:286027/140‑1 (MQ=255) ttccttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAg < 1:286063/140‑1 (MQ=255) ccttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATTATTGAAGCTGGCAAATACAATTTATCTTATca > 2:1103184/1‑140 (MQ=255) ccttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAgaa > 2:1856115/1‑138 (MQ=255) cttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTGGcac < 1:1202987/140‑1 (MQ=255) ttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTTTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGcaca < 2:2004336/140‑1 (MQ=255) ttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGcaca < 2:350317/140‑1 (MQ=255) ttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGcaca < 2:350370/140‑1 (MQ=255) ttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGcaca < 2:1360435/140‑1 (MQ=255) ttccATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGcaca < 2:1981773/140‑1 (MQ=255) tgctgATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCtcaa > 1:955992/1‑140 (MQ=255) gctgATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAAATTATCTTAGCACATTCACCCACTCtcaat > 2:1371794/1‑140 (MQ=255) ctgATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTTTAAAATATAATTGGATACATATAATCAAAATATTATTGAATCTGGCAAATAAAAATTATCTTAGCACATTCACCCAATAtcaatc > 1:2060445/1‑140 (MQ=255) ctgATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCtcaatc > 2:227084/1‑140 (MQ=255) ctgATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCtcaatc > 1:1667/1‑140 (MQ=255) ctgATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCtcaatc > 2:888378/1‑140 (MQ=255) ctgATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACAGATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCtcaatc > 1:2012177/1‑140 (MQ=255) ctgATTAATGAACGGCACCTCCAGAAAATATTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATTATTGAAGCTGGAAAATAAAATTTATCTTAGCACATTCACCCAATCtcaatc < 1:2047886/140‑1 (MQ=255) tCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGt < 1:1324647/140‑1 (MQ=255) tCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATAAATGATTGAAGCTGGCAAATACAATTTATCTTATCACATTCACCCAATCTCAAACAAGGt > 1:956484/1‑140 (MQ=255) tCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGt < 2:1782075/140‑1 (MQ=255) tCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGt < 1:1975834/140‑1 (MQ=255) cATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGtt < 1:664718/140‑1 (MQ=255) aaCGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTggg < 2:1972928/140‑1 (MQ=255) aaCGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTggg < 2:874895/140‑1 (MQ=255) aaTACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATc < 2:1738105/140‑1 (MQ=255) atatAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGc < 2:94421/140‑1 (MQ=255) gATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATAGGTTTTTTTGCTgttagtta > 1:453253/1‑140 (MQ=255) tACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCt < 2:280365/130‑1 (MQ=255) tACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCt > 1:280365/1‑130 (MQ=255) tACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCTGTTAGTTATc > 1:1209851/1‑140 (MQ=255) tACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGc < 2:1699480/129‑1 (MQ=255) tACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGc > 1:1699480/1‑129 (MQ=255) tACATATAATAAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCTGTTAGTTATc > 2:1828244/1‑140 (MQ=255) atatAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCACTCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAATTCGGTTTTTTTGCTGTTAGTTATCTaa > 1:575448/1‑140 (MQ=255) aTCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCTGTTAGTTATCTAAACGaa < 2:1667/140‑1 (MQ=255) tCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCTGTTAGTTATCTAAACGaaa > 2:1625933/1‑140 (MQ=255) tCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCTGGTATTTATCTAAACGaaa > 2:1151432/1‑140 (MQ=255) | GCTCCTGCAGCACCTCATCTATTCTTGACTGCTCTTCCTTCCATTCCTTATCCTGCTGATTCATGAACGGCACCTCCAGAAAATAGTTGACAAACACAGATGAAATACTGTAAAATATAATTGGATACATATAATCAATATATGATTGAAGCTGGCAAATACAATTTATCTTAGCACATTCACCCAATCTCAATCAAGGTTTGGGTTATTTATATGCAAAAAAACCGATTTCAATTTGAAATCGGTTTTTTTGCTGTTAGTTATCTAAACGAAA > NZ_CP034943/3244915‑3245188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |