Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP034943 132,185 G→A D560N (GAC→AAC)  fusA → elongation factor G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943132,1850GA95.8% 513.9 / 7.4 142D560N (GAC→AAC) fusAelongation factor G
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (86/50);  total (89/53)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 142 total aligned reads displayed.

AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGA  >  NZ_CP034943/132050‑132320
                                                                                                                                       |                                                                                                                                       
aaCGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACAt                                                                                                                                     >  2:311314/1‑140 (MQ=255)
          gcgcTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCaaaa                                                                                                                           >  2:1907483/1‑140 (MQ=255)
                 cttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCg                                                                                                                    <  1:116592/140‑1 (MQ=255)
                 cttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCg                                                                                                                    <  2:661552/140‑1 (MQ=255)
                 cttTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCg                                                                                                                    <  1:22722/140‑1 (MQ=255)
                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   <  1:1993067/140‑1 (MQ=255)
                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   <  2:903567/140‑1 (MQ=255)
                  tttgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   <  2:535380/140‑1 (MQ=255)
                    tgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGGCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATg                                                                                                                 >  2:606456/1‑140 (MQ=255)
                    tgaatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGAAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAATCAAAACTTTTCGATg                                                                                                                 >  2:132431/1‑140 (MQ=255)
                      aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGa                                                                                                                   <  2:502833/136‑1 (MQ=255)
                      aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGt                                                                                                               <  2:794495/140‑1 (MQ=255)
                      aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGt                                                                                                               >  2:485784/1‑140 (MQ=255)
                      aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGt                                                                                                               <  2:1608954/140‑1 (MQ=255)
                      aatttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACAGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGt                                                                                                               >  1:692266/1‑140 (MQ=255)
                         ttgaaAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCt                                                                                                            <  1:643006/140‑1 (MQ=255)
                              aaTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCa                                                                                                       <  2:308611/140‑1 (MQ=255)
                               ctGCGATTGTCGGTGGGGGAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAACTGGTGTACGGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACATTTCGATGGTTCTTACCat                                                                                                      <  2:434040/139‑1 (MQ=255)
                               aTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTc                                                                                                             <  2:1280006/133‑1 (MQ=255)
                               aTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTc                                                                                                             >  1:1280006/1‑133 (MQ=255)
                               aTGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCat                                                                                                      <  2:1374681/140‑1 (MQ=255)
                                                   gTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAt                                                                                  <  1:1883417/140‑1 (MQ=255)
                                                   gTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAt                                                                                  >  1:712330/1‑140 (MQ=255)
                                                      ccACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGc                                                                               >  2:2075569/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTCTCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAACTGGCAtt                                                                            >  2:1788412/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  2:1146431/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  1:866075/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  1:576855/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  1:1865754/1‑140 (MQ=255)
                                                         cGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCAtt                                                                            >  1:1951993/1‑140 (MQ=255)
                                                             aaTACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTaaa                                                                        >  1:961146/1‑140 (MQ=255)
                                                                   tCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgct                                                                  <  2:1917612/140‑1 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAATCAAAACTTTTCGATGGTTATTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 >  1:1641622/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 >  1:1516481/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 >  1:441868/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 >  2:373148/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 >  2:104862/1‑140 (MQ=255)
                                                                    cccAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTAATGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTtgctg                                                                 >  2:926276/1‑140 (MQ=255)
                                                                      cAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTgctgct                                                               >  1:524814/1‑140 (MQ=255)
                                                                       aGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCtt                                                              >  1:1130603/1‑140 (MQ=255)
                                                                         cTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCt                                                            >  1:129283/1‑140 (MQ=255)
                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGACATGGCATTTAAAGTTGCTGCTTCTATg                                                         >  2:345174/1‑140 (MQ=255)
                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                         >  1:796197/1‑140 (MQ=255)
                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                         >  2:437427/1‑140 (MQ=255)
                                                                            ttCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATg                                                         >  2:316837/1‑140 (MQ=255)
                                                                              cAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGc                                                       >  2:1600195/1‑140 (MQ=255)
                                                                                 gCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                                                    >  2:1894744/1‑140 (MQ=255)
                                                                                 gCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCAtt                                                    >  2:1515870/1‑140 (MQ=255)
                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                               >  2:1529250/1‑140 (MQ=255)
                                                                                      tCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTACTGCTTCTATGGCATTGaaaa                                               >  2:2020486/1‑140 (MQ=255)
                                                                                      tCTGGAAGATGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGaaaa                                               >  1:176742/1‑140 (MQ=255)
                                                                                         ggAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATg                                            <  2:884428/140‑1 (MQ=255)
                                                                                           aaGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa                                          <  1:299855/140‑1 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTCAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:374919/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCa                                          <  1:1831451/136‑1 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:526507/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  1:56557/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:526553/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  1:2087345/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:1733246/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:1753932/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:432835/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:1984159/1‑140 (MQ=255)
                                                                                               tGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAAAGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:757757/1‑140 (MQ=255)
                                                                                               tGCACTTAAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCa                                      >  2:1482196/1‑140 (MQ=255)
                                                                                                gCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg                                     >  1:1782885/1‑140 (MQ=255)
                                                                                                gCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAg                                     >  2:728129/1‑140 (MQ=255)
                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                    <  1:104862/140‑1 (MQ=255)
                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                    <  1:728129/140‑1 (MQ=255)
                                                                                                 cACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                    <  1:311314/140‑1 (MQ=255)
                                                                                                 cACTTGAAAAGGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGc                                    <  1:1211064/140‑1 (MQ=255)
                                                                                                  aCTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCa                                   >  2:1781370/1‑140 (MQ=255)
                                                                                                    ttGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCaaa                                 >  1:900159/1‑140 (MQ=255)
                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                               <  2:953446/140‑1 (MQ=255)
                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                               <  2:672112/140‑1 (MQ=255)
                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                               <  2:165201/140‑1 (MQ=255)
                                                                                                      gAAAATGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATg                               <  2:1182787/140‑1 (MQ=255)
                                                                                                           tGGTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAAcc                          <  1:751203/140‑1 (MQ=255)
                                                                                                            ggTGTACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAAccc                         >  1:1348961/1‑140 (MQ=255)
                                                                                                              tgtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAg                       >  1:993533/1‑140 (MQ=255)
                                                                                                               gtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                      <  2:1964711/140‑1 (MQ=255)
                                                                                                               gtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                      <  2:1906674/140‑1 (MQ=255)
                                                                                                               gtACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGt                      <  2:1134610/140‑1 (MQ=255)
                                                                                                                tACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                     >  1:1120012/1‑140 (MQ=255)
                                                                                                                tACTGGCAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGtt                     >  1:477091/1‑140 (MQ=255)
                                                                                                                      cAGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTcttctt               >  1:143679/1‑140 (MQ=255)
                                                                                                                       aGGCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTg              >  2:161428/1‑140 (MQ=255)
                                                                                                                        ggCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCCTTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa             >  1:484898/1‑140 (MQ=255)
                                                                                                                        ggCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa             >  2:1082011/1‑140 (MQ=255)
                                                                                                                        ggCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGa             >  2:289917/1‑140 (MQ=255)
                                                                                                                         gCTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGaa            <  2:1575733/140‑1 (MQ=255)
                                                                                                                          cTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCa                                                      >  2:2054698/1‑97 (MQ=255)
                                                                                                                          cTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc           >  2:1302463/1‑140 (MQ=255)
                                                                                                                          cTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc           <  2:576855/140‑1 (MQ=255)
                                                                                                                          cTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc           <  2:1951993/140‑1 (MQ=255)
                                                                                                                          cTTCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAAc           >  2:1958530/1‑140 (MQ=255)
                                                                                                                            tCCCGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCa         <  1:1984159/140‑1 (MQ=255)
                                                                                                                             cccGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCaa        >  1:650544/1‑140 (MQ=255)
                                                                                                                               cGCTTATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATc      >  1:1575285/1‑140 (MQ=255)
                                                                                                                                   tATCAACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGa  >  2:2025656/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AACGAAGAAGGCGCTTGCTTTGAATTTGAAAATGCGATTGTCGGTGGGGTAGTTCCACGTGAATACATCCCAGCTGTTCAAGCAGGTCTGGAAGATGCACTTGAAAATGGTGTACTGGCAGGCTTCCCGCTTATCGACATTAAAGCAAAACTTTTCGATGGTTCTTACCATGATGTTGACTCAAACGAAATGGCATTTAAAGTTGCTGCTTCTATGGCATTGAAAAATGCAGTCAGCAAATGTAACCCAGTTCTTCTTGAACCAATCATGA  >  NZ_CP034943/132050‑132320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: