Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,293,3480GT24.7% 314.3 / 13.3 155V141L (GTA→TTA) EO946_RS06740sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base G (79/37);  new base T (0/38);  total (80/75)
Fisher's exact test for biased strand distribution p-value = 1.98e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 157 total aligned reads displayed.

GCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACACATA  >  NZ_CP034943/1293209‑1293486
                                                                                                                                           |                                                                                                                                          
gCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGgtg                                                                                                                                            >  1:658569/1‑140 (MQ=255)
    gacggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaa                                                                                                                                        >  2:1188909/1‑140 (MQ=255)
           aGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCg                                                                                                                                 >  1:833469/1‑140 (MQ=255)
            gttATCATAATAAGACATAGAGAGAACCCAACAAGCGTTATCCCTGTTAAGGGGCAGAGAAAAAGAATTGTTTTTGCGGATTAAGTTTTATTGATTTTCTTATTAACTAATCGCTTATCTTCTCGTTTTAAAAAAATCGa                                                                                                                                <  1:2106423/138‑1 (MQ=255)
                  agaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGc                                                                                                                          >  2:2516494/1‑140 (MQ=255)
                   gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                                                         >  2:1064274/1‑140 (MQ=255)
                       aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                                     >  2:739266/1‑140 (MQ=255)
                       aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                                                     >  1:889846/1‑140 (MQ=255)
                         gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAc                                                                                                                   >  1:235475/1‑140 (MQ=255)
                            agagagagAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTGGCTGCATAAGGTAGAATGCTTTTCTTATGAAAGAATCTCTGATCTTCTCGGTGTAAAAAAATCGACTGTGAAAACGACCAt                                                                                                                >  1:1458981/1‑140 (MQ=255)
                             gagagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATc                                                                                                               >  1:72829/1‑140 (MQ=255)
                              agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                                                              >  1:1579850/1‑140 (MQ=255)
                               gagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACAATCaa                                                                                                             >  2:30352/1‑140 (MQ=255)
                                    actcaaagaagCTTTATCCCTTTTAAGGGGCAGATAAAAAGAAATTTTTTTGCTGCATACGGTATAATGCTTTTATTATGAACCAATCGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                                                        <  2:1956258/135‑1 (MQ=255)
                                    aaTCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCTACTGTGCAAACGACCATCAAACgcg                                                                                                        >  2:2269743/1‑138 (MQ=255)
                                    aaTCAAAGAAGCTTTATCCCTGTTAACGGGCAGAGAAAAATAAATGTTTTTGCTGAATACGGTAGAATGCTTTTATTATGAACGAATCGCTTATCTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                                                        <  2:3135/140‑1 (MQ=255)
                                    aaTCAAAGAAGCTTTATCCCTGTTAACGGGAAGATAAAAATAAATGTTTTTGATGAATATGGTAGAATGCTTTTCTTATCAACCAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                                                        <  2:2509681/140‑1 (MQ=255)
                                     agcaaacaagCGTTATCCCTGTTAACGGGCACAGAAAAAGATATGTTTTTGCTCAATACGGTACAATGTTTTTCTTATCAACCAATCGCTTATCTTCTGGTTGAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGc                                                                                                       <  1:209564/133‑1 (MQ=255)
                                      tCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCg                                                                                                      >  1:1199476/1‑140 (MQ=255)
                                      tCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCg                                                                                                      >  1:1305572/1‑140 (MQ=255)
                                      ccaaagaagCTTATTCCCTGTTTAGCGGAAGATAAAAAAAAATTTATTTGATGCATAAGCAAGAATCATTTATTTTTGAATGACTCGGTGTTCTTCTCGGTGT‑AAAAAATCGACTGTGCAAACTACCATCAAACGGGCGa                                                                                                     <  2:1096489/137‑1 (MQ=255)
                                          agaagCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCg                                                                                                  >  1:2630367/1‑140 (MQ=255)
                                          agaagCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCg                                                                                                  >  1:98584/1‑140 (MQ=255)
                                              gctttATCCCTGTTAACGGGAATATAAAAATAAATGTTTTTGCTGAATAAGGTAGAATACTTTTCTTATGACCGAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                                                              <  1:2838470/137‑1 (MQ=255)
                                              gCGTTAGCCGTGTTAACGGGCATAGAAAAAGAATTGTTTTTGGTGATTAAGGTAGATTGCTTTTCTTATCACCGACTCGCTGATCTTGTCGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                                                              <  2:1018588/140‑1 (MQ=255)
                                               cGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAg                                                                                             >  1:2415521/1‑140 (MQ=255)
                                               cGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAg                                                                                             >  1:862315/1‑140 (MQ=255)
                                                 ttATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGAt                                                                                           >  2:1361896/1‑140 (MQ=255)
                                                 ttATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGAt                                                                                           >  1:1200914/1‑140 (MQ=255)
                                                      cattttAACGGTCAGAGAAAAAGAAATTTTTTTGCTGAATAAGGTAGAATGCTTTTCTTATCAACTAATCGCTTATCTTGTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCaaa                                                                                      <  2:1466338/136‑1 (MQ=255)
                                                         gTTAACGGGCAGAGCAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATATTCTCGCGGGAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  1:1580002/1‑140 (MQ=255)
                                                         gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  1:2171599/1‑140 (MQ=255)
                                                         gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                                                   >  1:286364/1‑140 (MQ=255)
                                                         gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCCCTGATCTTCTCGGTCTAAAAAAATCGACTGTGCAAACGACCATCAAACGGCCGAACGTAAAGATCCAAAGAc                                                                                   >  1:2693171/1‑140 (MQ=255)
                                                              cGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAcagcag                                                                              >  2:1145802/1‑140 (MQ=255)
                                                               gggCAGATAAAAATAAATTTTTTTGCTGAATAAGTTATAATGCTTTTATTATCAACTAATGGTTGATCTTCTCGTTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagg                                                                             <  1:2494205/140‑1 (MQ=255)
                                                                ggCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagga                                                                            >  2:499174/1‑140 (MQ=255)
                                                                    gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                                                                        >  1:1194447/1‑140 (MQ=255)
                                                                    gagaAAAAGAAATGTTTTTGCTGCAGACGGTAGACTGGTTTGCTTATGAACGAATCGCTGAGCTTCTCGGTGTAAAAAAATCGACTGTGCTAACGACCATCAAACGGGCGAGCGTAAATATGCAACGCCAGCAGGAGGaa                                                                        >  1:2607669/1‑140 (MQ=255)
                                                                               aTGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAatcgatc                                                             >  1:663415/1‑140 (MQ=255)
                                                                                  tttttGCTTCATATGGTTTATTGCTTTTCTTATTAACGATTCGATTATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                                                          <  2:263275/140‑1 (MQ=255)
                                                                                         tgcATAGGGTAGAATGTTTTTCTTATTACCGAATCGCTGATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGa                                                   <  1:298277/140‑1 (MQ=255)
                                                                                             tAATGTAGTATGCTTTTCTTTTTAACGAATCGATTATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                                               <  2:979208/140‑1 (MQ=255)
                                                                                             acaGGTCGAATGCTTTTCTTATCAACGAATCGCTGATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc                                               <  2:2031664/138‑1 (MQ=255)
                                                                                                  tcgAATGCTTGTCTTATGAACGACTCGCTGATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTc                                          <  2:216824/138‑1 (MQ=255)
                                                                                                    gAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCAt                                        >  1:542108/1‑140 (MQ=255)
                                                                                                    gAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCAt                                        >  1:1401458/1‑140 (MQ=255)
                                                                                                    gAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCAt                                        >  1:2360792/1‑140 (MQ=255)
                                                                                                     aaTGTTTTTCTTATTAACTAATGGCTGATCTTGTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                                       <  1:1263402/140‑1 (MQ=255)
                                                                                                     aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                                       >  1:2173718/1‑140 (MQ=255)
                                                                                                     aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                                       >  1:2289224/1‑140 (MQ=255)
                                                                                                     aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa                                       >  1:2443591/1‑140 (MQ=255)
                                                                                                     aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATAAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATAGCTTGCCTGAAAGCTTGTCATa                                       >  1:1476862/1‑140 (MQ=255)
                                                                                                          ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAACGACAGCAGGAGGAAATGAATCGATCGCTTGCCGGAAAGCTTGTCATACGttt                                  >  2:1374472/1‑140 (MQ=255)
                                                                                                          ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  2:1752366/1‑140 (MQ=255)
                                                                                                          ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  1:2371445/1‑140 (MQ=255)
                                                                                                          ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  1:1243891/1‑140 (MQ=255)
                                                                                                          ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  1:2778369/1‑140 (MQ=255)
                                                                                                          ttttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGttt                                  >  1:2822783/1‑140 (MQ=255)
                                                                                                           ttttttATGAACTAATCTCTGATTTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:2761827/140‑1 (MQ=255)
                                                                                                           tttCTTTTTACCGATTCGGTGATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:2220678/140‑1 (MQ=255)
                                                                                                           tttCTTATTACAGAATGGTTTATATTCTGGTTGTAAAAAACTCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:2269743/140‑1 (MQ=255)
                                                                                                           tttCTTATTAACTAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  1:739266/140‑1 (MQ=255)
                                                                                                           tttCTTATGAACTAATCGCTGATATTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:845445/140‑1 (MQ=255)
                                                                                                           tttATTCTGAACGACTCGCTGATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:889846/140‑1 (MQ=255)
                                                                                                           tttATTATTAACGAATCGTTTATATTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTg                                 <  2:2096048/140‑1 (MQ=255)
                                                                                                            ttCTTATTAACTAATCGCTTATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc                                <  2:235475/140‑1 (MQ=255)
                                                                                                            ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGc                                >  1:4652/1‑140 (MQ=255)
                                                                                                            ttCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTCTgt                                >  1:2031664/1‑139 (MQ=255)
                                                                                                             tCTTTTGAACGAATCGCTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGcc                               <  2:833880/140‑1 (MQ=255)
                                                                                                                tATTAACTAATCGCTTATCTTATCTGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCAcc                            <  2:2363668/140‑1 (MQ=255)
                                                                                                                tATGAACGACTCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAAAGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCAcc                            <  2:334376/140‑1 (MQ=255)
                                                                                                                aaTGAACCAACCGCTCATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCAcc                            <  1:1188909/139‑1 (MQ=255)
                                                                                                                 aTGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCt                           >  1:2564990/1‑140 (MQ=255)
                                                                                                                 aTGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAAAGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGATTGTCATACGTTTGACACCt                           >  1:1701256/1‑140 (MQ=255)
                                                                                                                  ttaaCTAATCGCTTATCTTCTGGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                          <  1:2491715/138‑1 (MQ=255)
                                                                                                                  ggAACTATTCGTTTATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCTATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCta                          <  2:62610/139‑1 (MQ=255)
                                                                                                                    aaCGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCtata                        <  2:663415/140‑1 (MQ=255)
                                                                                                                     agtaaTCTCTGATATTATCTGTTTAAAAAAATCGACTGTGCAAAATACCATCAAAAGGGCTATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGATTGCCTGAATGATTGTCATACGTTTGCCACCTATaa                       <  2:1646531/137‑1 (MQ=255)
                                                                                                                     aggAATCGTTGTTCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  2:2693171/138‑1 (MQ=255)
                                                                                                                     actaaTCGCTTATGTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  2:1579850/137‑1 (MQ=255)
                                                                                                                     actaaTCGATGATATTATCTGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAATCTTGTCATACGTTTGCCACCTATaa                       <  2:2837546/137‑1 (MQ=255)
                                                                                                                     aCGAATCGCTGATCTTCTCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATaa                       <  1:1361896/140‑1 (MQ=255)
                                                                                                                         aTCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGa                   >  1:2570950/1‑140 (MQ=255)
                                                                                                                          ttgCTCATCTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTTaa                  <  2:772728/138‑1 (MQ=255)
                                                                                                                          tCGCTTATCTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGaa                  <  1:1079713/140‑1 (MQ=255)
                                                                                                                          tCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGaa                  <  2:2630367/140‑1 (MQ=255)
                                                                                                                                 ttttctCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa           <  2:2171599/138‑1 (MQ=255)
                                                                                                                                 tcttctCGTTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa           <  1:1133968/140‑1 (MQ=255)
                                                                                                                                 tcttctCGGTTAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa           <  1:310441/140‑1 (MQ=255)
                                                                                                                                 tattctCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCa           <  1:2088478/138‑1 (MQ=255)
                                                                                                                                  tttctCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCAt          <  2:1199476/139‑1 (MQ=255)
                                                                                                                                  cttctCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCAt          <  2:4652/140‑1 (MQ=255)
                                                                                                                                  cttctCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCAt          <  2:1305572/140‑1 (MQ=255)
                                                                                                                                  cttctCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCAt          <  2:1305646/140‑1 (MQ=255)
                                                                                                                                     ctcGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTTAATAGAGCATGac       >  2:14237/1‑140 (MQ=255)
                                                                                                                                     ctcGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGac       >  2:2390056/1‑140 (MQ=255)
                                                                                                                                     ctcGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGac       >  2:918726/1‑140 (MQ=255)
                                                                                                                                     ctcGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGCCGATCGTAAAGGTGCAAACACAGCAGCAGCACATCAATCGATCGCTTGCCTGAAAGCATGTCATACGTAAGCCACCTGTAAGTGAATATAGCATGac       >  2:2781124/1‑140 (MQ=255)
                                                                                                                                     ctcGGTGTAAAAAAATCGACTGTGCAAAAGACCAACAAACGGGCGATCGTAAAGATGCAAAGACACCAGGAGGAAAAGAAACGAACGCTTGCCTGAAAGCATTTCATACGTTTGCCACCTATAAGTGAATAGAGCATGac       >  1:1882495/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACGTTTGCCACCTATAAGTGAATAGAGCATGACACATA  >  NZ_CP034943/1293209‑1293486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑