Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2270TG33.6% 159.2 / 40.4 126intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base T (29/54);  new base G (42/0);  total (71/55)
Fisher's exact test for biased strand distribution p-value = 2.63e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 130 total aligned reads displayed.

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363
                                                                                                                                          |                                                                                                                                         
atatGATCAGCTGGAAAGCTCTAAAGCTTACTATGACTGGCTGAAACGGCTTGCTGAATCGGTGCAGACCGGAGAAATTTTTAAATTTATACCGGTCGAAACAAAGTAAAGCAGAAA‑ACGCCTGGGAAACTAGGCGtttt                                                                                                                                         >  1:1395527/1‑140 (MQ=255)
ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGtgttt                                                                                                                                        >  2:2114675/1‑140 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGtgtttttt                                                                                                                                     >  1:938496/1‑139 (MQ=255)
   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGAGTGAAACAAAGTAATACA‑AAA‑GCGCCTGGGAAACCGGGCGttttttgt                                                                                                                                     >  2:92942/1‑138 (MQ=255)
     accAACTGCAAAGCTCTAACGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                   <  2:938496/138‑1 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                   >  2:1364233/1‑140 (MQ=255)
     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                   >  2:2188243/1‑140 (MQ=255)
     aGCAACTGGAAAGCACTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                   >  2:681524/1‑140 (MQ=255)
      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGttt                                                                                                                                  >  2:2134997/1‑139 (MQ=255)
       cAACTGGAAAGCTATAACGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCCGCGCGGGGAAATTTTAAAATTTATCCCGATTCAAACAAAGAAACACA‑AAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCt                                                                                                                                 <  1:889525/140‑1 (MQ=255)
        aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTAtt                                                                                                                                >  1:535652/1‑140 (MQ=255)
         agtGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCttt                                                                                                                               <  2:1734223/138‑1 (MQ=255)
                aGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGt                                                                                                                        >  2:275906/1‑140 (MQ=255)
                  ctctCAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTATGTaa                                                                                                                      >  2:1613705/1‑140 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTaa                                                                                                                      >  2:1890590/1‑140 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTaa                                                                                                                      >  2:1324972/1‑140 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGttttttttttta                                                                                                                      >  1:1903605/1‑134 (MQ=255)
                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTaa                                                                                                                      >  1:1493250/1‑140 (MQ=255)
                    ctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAAtt                                                                                                                    >  1:158329/1‑140 (MQ=255)
                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑TCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTAttt                                                                                                                   >  2:2059999/1‑139 (MQ=255)
                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTAATta                                                                                                                   >  1:290967/1‑140 (MQ=255)
                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAAAA‑AAA‑ACGCCTGGGAAAACGGGCGTTTTTTTGTTTTTTTTTATTttatta                                                                                                                   >  2:1488302/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTAATAaaaa                                                                                                            >  2:1850530/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTACTACaaa                                                                                                            >  2:1170309/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGTAATTAATAaaaa                                                                                                            >  2:1832056/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGTAATTAATACaaa                                                                                                            >  2:1850234/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTTATTAATAaaaa                                                                                                            >  1:2090291/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATAAATAaaaa                                                                                                            >  1:19144/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAAATACTACaaa                                                                                                            >  2:2369181/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTTTGTAATTAATACaaa                                                                                                            >  1:1205990/1‑140 (MQ=255)
                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAAACGGGCGTTTTTTTGTTGTTTTTTATTTAAATAATAaaaa                                                                                                            >  2:357947/1‑140 (MQ=255)
                             aCTCTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTGTTTTTTTTTTTTTTTTTTATTAATAaaaaa                                                                                                           >  1:783800/1‑139 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAAATAATAaaaaa                                                                                                           >  1:681692/1‑139 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTTATTACTACAAAt                                                                                                           >  1:1531768/1‑140 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTCTGTAATTACTACAAAt                                                                                                           >  2:2108361/1‑140 (MQ=255)
                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAATACaaaa                                                                                                           >  2:2035698/1‑139 (MQ=255)
                               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTATTTAATTACTAAAAAttt                                                                                                         >  2:1065755/1‑140 (MQ=255)
                               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATTTAATTAAAAAAAAttt                                                                                                         >  2:828133/1‑140 (MQ=255)
                               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAGTACAAAatt                                                                                                         >  1:142476/1‑140 (MQ=255)
                                  gATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑aa                                                                                                     <  2:915300/140‑1 (MQ=255)
                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTACTACAAAATTT‑aaa                                                                                                    >  1:2281400/1‑140 (MQ=255)
                                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTACTAAAAATTTT‑aaaa                                                                                                   >  1:128495/1‑140 (MQ=255)
                                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTTTTTTTTATGTAATTAATACAAATTTT‑AAAAATa                                                                                                >  2:503598/1‑140 (MQ=255)
                                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAATACAAATTTT‑AAAAATa                                                                                                >  2:327750/1‑140 (MQ=255)
                                         tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATGTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTGTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATaag                                                                                              >  2:1378282/1‑138 (MQ=255)
                                           aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTAATTACTAAAAATTTG‑TAAAATACggg                                                                                            >  2:2093592/1‑140 (MQ=255)
                                               ggCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCTATTGAACCAAAGTAAAACA‑AAA‑ACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTG‑AAAAATACGGGaaaa                                                                                        <  2:720525/140‑1 (MQ=255)
                                               ggCTTGCTGAATCGGTGCAGAGCCGGGAAATTTTTAAATTTATACCGATTTAAAAAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGATTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTG‑AAAAATACGGGaaaa                                                                                        <  2:721003/140‑1 (MQ=255)
                                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAAc                                                                                      <  1:605243/140‑1 (MQ=255)
                                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAATTACTACAAAATTT‑TAAAATAACGGACAAAc                                                                                      >  1:2022610/1‑140 (MQ=255)
                                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTTTGTAAATAGAAAAAATTTGTAAAAAAACGGCaaaaa                                                                                       >  1:445843/1‑140 (MQ=255)
                                                  ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAATTAAAACAAAATTG‑AAAAAAAAGGGgaaaaaa                                                                                     >  1:2793072/1‑138 (MQ=255)
                                                   tGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTGTTTTGGTGATTTTTTTGTAAATAATAAAAATTTG‑AAAAAAAACGGaaaaaaaa                                                                                    >  2:1425971/1‑137 (MQ=255)
                                                              tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTAATTAATAAAAATTTT‑AAAAATACTGGAAAAAAATCACAGTTACtt                                                                         >  2:1126555/1‑140 (MQ=255)
                                                                 cgcggggggAAATTTTTAAATTTATACCGATTGAAACAACGTAAACAA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAACCATCCCATTTACTTCCa                                                                      <  1:344770/135‑1 (MQ=255)
                                                                 agcgCGGGCAAATTTCAAAATTTAAACCGATTCAAACAAACTAACACA‑AAA‑ACGCCGGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                      <  1:2438416/137‑1 (MQ=255)
                                                                 agagCGGGGAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                      <  1:2035698/140‑1 (MQ=255)
                                                                        gtaaaTTTTTAAATTTATACCGATTGAAAAAAAGTAAAAAA‑AAA‑ACGCCTGGGAAACCGGGCGTCTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAtt                                                               <  1:1034386/138‑1 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTACTACCAATTTG‑TAAAATAAGGGAAAAACATCCCATTTAATTCCAAAATAtt                                                               >  2:2797176/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTGTTTTTTGTAAATACTACAAATTTT‑AAAAATAAGGGAAAAAAAAGACAATTAATTACAAAATAtt                                                               >  2:1524849/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTCTTTTTTATGTAATTACTAAAAATTTG‑TAAAATACGGGAAAAACATCCCATTTAGTTCCAAAAtttt                                                               >  2:2026476/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAATACAAAATTG‑AAAAAAACGGGAAAAACATCCCATTTAATTCAAAAAAAtt                                                               >  2:1687221/1‑140 (MQ=255)
                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTAGTTCTTTTTTATGTGATTACTACAAATTTG‑AAAAATAAGTGACAAACATACCAATTCAGTACAAAatata                                                               >  2:2639383/1‑139 (MQ=255)
                                                                              ttttAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  1:1811155/140‑1 (MQ=255)
                                                                              ttttAAATTAATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  1:266976/140‑1 (MQ=255)
                                                                              ttatAAATTTATACCGATTCAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  1:2134997/137‑1 (MQ=255)
                                                                                        ataCCGATTTAAACAACGAAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTCCAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                               <  2:486253/140‑1 (MQ=255)
                                                                                        ataCCGATTGAAAAAAAGAAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                               <  2:1918191/140‑1 (MQ=255)
                                                                                             gATTCAAACAAAGTAAACCA‑AAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                          <  1:2369181/140‑1 (MQ=255)
                                                                                               ttGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTGTTTTGTTCTGTTTTTTGTAATTACTACAAAATTG‑AAAAATACGGGGAAAAAATGCCAGTTTCTTCCCAAAGATTTGATTGTAATTTTTTGTAGGtga                                        >  2:885271/1‑138 (MQ=255)
                                                                                                  aaacaaacAACAACA‑CAA‑ACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATACCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:1832056/140‑1 (MQ=255)
                                                                                                        aGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACTAAAAAATTT‑AAAAATAAGGGTAAAAAAAACCATTTAGTTACAAAATATTTGTTGGTAATTTTATGTTTGTAAAATTTTTAt                               >  2:1410815/1‑140 (MQ=255)
                                                                                                        aGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTGTTTTATTTTTTATGTAATTATGAAAAAATTT‑AAAAATAAGGGAAAAACATACCCTTTACTTCCAAAATATTTTATGTTAATTTTATTTAGTTAAAAttttttt                               >  2:2464142/1‑136 (MQ=255)
                                                                                                        aGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTGTTTTTTTGTTTTTTATGTAATTACTAAAAATTTG‑TAAAATAAGGGAAAAACATCCCCTTTTATTCCCAAATATTTGTTTGTAATTTTATGTATGTGAAAttgtgtg                               >  2:1579105/1‑135 (MQ=255)
                                                                                                        aGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTTGTTTTTTTGTTATTACTAAAAAATTT‑AAAAATAAGGGAAAAACATAACAGTTAATTGAAAAAAATTTTTTTTTTATTTTATTTAGTTTATAtttttgt                               >  1:1981489/1‑136 (MQ=255)
                                                                                                        aGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAATTAATACAAATTTG‑TAAAATAAGGGAAAAACATACCAATTAATTAAAAAAAATTTTATGGTAATTTTATTTAGGTaaaaataagta                               >  2:495376/1‑133 (MQ=255)
                                                                                                        aGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAAATAATAAAAAATTT‑AAAAATAAGGGAAAAAAATACCATTTAATTACAAAAAATTTTATGGTAATTTTATTTAGGTAAAATTTTTAt                               >  2:1481878/1‑140 (MQ=255)
                                                                                                            acaca‑aca‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATGTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:7907/138‑1 (MQ=255)
                                                                                                            aaaca‑aac‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2797176/140‑1 (MQ=255)
                                                                                                            aaaaa‑aaa‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1558349/140‑1 (MQ=255)
                                                                                                            aaaaa‑aaa‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:14805/140‑1 (MQ=255)
                                                                                                            aaaaa‑aaa‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:290967/140‑1 (MQ=255)
                                                                                                            aaaaa‑aaa‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1078721/140‑1 (MQ=255)
                                                                                                            aaaCA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1691981/140‑1 (MQ=255)
                                                                                                            aaaCA‑AAA‑ACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:127825/140‑1 (MQ=255)
                                                                                                             aaCA‑AAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1850234/140‑1 (MQ=255)
                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:1481878/140‑1 (MQ=255)
                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:19144/140‑1 (MQ=255)
                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:1410815/140‑1 (MQ=255)
                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:384362/140‑1 (MQ=255)
                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:2818005/140‑1 (MQ=255)
                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:2093592/140‑1 (MQ=255)
                                                                                                                             tgAAAGCGTGCGTTTTTTTTTTCTTTTTTATTTAATTAATACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt            <  1:1687221/139‑1 (MQ=255)
                                                                                                                             gcaaaCCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACGTCCAAACTATTTGAGGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt            <  1:2279903/138‑1 (MQ=255)
                                                                                                                                  ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa       <  1:1613705/140‑1 (MQ=255)
                                                                                                                                  ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa       <  1:1065755/140‑1 (MQ=255)
                                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACTACATATTTG‑AAAAATACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:2753451/140‑1 (MQ=255)
                                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AACAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1496381/140‑1 (MQ=255)
                                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:503598/140‑1 (MQ=255)
                                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:681692/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACA‑AAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑