Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2360CG40.6% 151.0 / 45.2 120intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base C (9/54);  new base G (43/0);  total (66/54)
Fisher's exact test for biased strand distribution p-value = 3.84e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 121 total aligned reads displayed.

GCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991097‑2991363
                                                                                                                                             |                                                                                                                                
gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTc                                                                                                                                  >  2:1811155/1‑140 (MQ=255)
 cAACTGGAAAGCTATAACGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCCGCGCGGGGAAATTTTAAAATTTATCCCGATTCAAACAAAGAAACACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCt                                                                                                                                 <  1:889525/140‑1 (MQ=255)
  aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAAGCGGGCGGTTTTTTGTTCtt                                                                                                                                >  2:2147420/1‑140 (MQ=255)
  aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTAtt                                                                                                                                >  1:535652/1‑140 (MQ=255)
   agtGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCttt                                                                                                                               <  2:1734223/138‑1 (MQ=255)
          aGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGt                                                                                                                        >  2:577956/1‑140 (MQ=255)
          aGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGt                                                                                                                        >  2:275906/1‑140 (MQ=255)
            ctctCAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTATGTaa                                                                                                                      >  2:1613705/1‑140 (MQ=255)
            ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTaa                                                                                                                      >  2:1890590/1‑140 (MQ=255)
            ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTaa                                                                                                                      >  2:1324972/1‑140 (MQ=255)
            ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGttttttttttta                                                                                                                      >  1:1903605/1‑134 (MQ=255)
            ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTaa                                                                                                                      >  1:1493250/1‑140 (MQ=255)
              ctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAAtt                                                                                                                    >  1:158329/1‑140 (MQ=255)
               tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAATCGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTAttt                                                                                                                   >  2:2059999/1‑139 (MQ=255)
               tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTttatta                                                                                                                   >  1:2284723/1‑140 (MQ=255)
               tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTAATta                                                                                                                   >  1:290967/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTAATAaaaa                                                                                                            >  2:1850530/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTACTACaaa                                                                                                            >  2:1170309/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGTAATTAATACaaa                                                                                                            >  2:1850234/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTTATTAATAaaaa                                                                                                            >  1:2090291/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTTAATAATAaaaa                                                                                                            >  1:14805/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATAAATAaaaa                                                                                                            >  1:19144/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAAATACTACaaa                                                                                                            >  2:2369181/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTTTGTAATTAATACaaa                                                                                                            >  1:1205990/1‑140 (MQ=255)
                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAAACGGGCGTTTTTTTGTTGTTTTTTATTTAAATAATAaaaa                                                                                                            >  2:357947/1‑140 (MQ=255)
                       aCTCTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTTTTTTTTTTTTTTTTATTAATAaaaaa                                                                                                           >  1:783800/1‑139 (MQ=255)
                       aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTTATTACTACAAAt                                                                                                           >  1:1531768/1‑140 (MQ=255)
                       aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTCTGTAATTACTACAAAt                                                                                                           >  2:2108361/1‑140 (MQ=255)
                       aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAATACaaaa                                                                                                           >  2:2035698/1‑139 (MQ=255)
                         tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTATTTAATTACTAAAAAttt                                                                                                         >  2:1065755/1‑140 (MQ=255)
                         tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATTTAATTAAAAAAAAttt                                                                                                         >  2:828133/1‑140 (MQ=255)
                         tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAGTACAAAatt                                                                                                         >  1:142476/1‑140 (MQ=255)
                            gATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGaa                                                                                                     <  2:915300/140‑1 (MQ=255)
                            gATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGaa                                                                                                     <  2:915166/140‑1 (MQ=255)
                             aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTACTACAAAATTTaaa                                                                                                    >  1:2281400/1‑140 (MQ=255)
                              ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTACTAAAAATTTTaaaa                                                                                                   >  1:128495/1‑140 (MQ=255)
                                 gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAATACAAATTTTAAAAATa                                                                                                >  2:327750/1‑140 (MQ=255)
                                 gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTAAAAAAAACAAATTTAAAAAATa                                                                                                >  2:1496381/1‑140 (MQ=255)
                                     aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTAATTACTAAAAATTTGTAAAATACggg                                                                                            >  2:2093592/1‑140 (MQ=255)
                                       aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTGTGTTGTTTTTTTTGTTATTACTAAAAAATTTAAAAATAAGGGaa                                                                                          >  1:2012895/1‑140 (MQ=255)
                                         ggCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCTATTGAACCAAAGTAAAACAAAAACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTGAAAAATACGGGaaaa                                                                                        <  2:720525/140‑1 (MQ=255)
                                         ggCTTGCTGAATCGGTGCAGAGCCGGGAAATTTTTAAATTTATACCGATTTAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGATTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTGAAAAATACGGGaaaa                                                                                        <  2:721003/140‑1 (MQ=255)
                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAAc                                                                                      <  1:605243/140‑1 (MQ=255)
                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAATTACTACAAAATTTTAAAATAACGGACAAAc                                                                                      >  1:2022610/1‑140 (MQ=255)
                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTTTGTAAATAGAAAAAATTTGTAAAAAAACGGCaaaaa                                                                                       >  1:445843/1‑140 (MQ=255)
                                            ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAATTAAAACAAAATTGAAAAAAAAGGGgaaaaaa                                                                                     >  1:2793072/1‑138 (MQ=255)
                                             tGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGGTGATTTTTTTGTAAATAATAAAAATTTGAAAAAAAACGGaaaaaaaa                                                                                    >  2:1425971/1‑137 (MQ=255)
                                               cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTAATAAAAAAAATAAAAATACTGGaaaaaaaaca                                                                                  >  1:1691981/1‑135 (MQ=255)
                                                           cgcggggggAAATTTTTAAATTTATACCGATTGAAACAACGTAAACAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAACCATCCCATTTACTTCCa                                                                      <  1:344770/135‑1 (MQ=255)
                                                           agcgCGGGCAAATTTCAAAATTTAAACCGATTCAAACAAACTAACACAAAAACGCCGGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                      <  1:2438416/137‑1 (MQ=255)
                                                           agagCGGGGAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                      <  1:2035698/140‑1 (MQ=255)
                                                                  gtaaaTTTTTAAATTTATACCGATTGAAAAAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTCTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAtt                                                               <  1:1034386/138‑1 (MQ=255)
                                                                  ggAAATTTTTAAATTTATACCGGTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATTTTATTACTACCAATTTTAAAAATTCGGGAAAAAAATCCCAGTTACTTGCAAAATGtt                                                               >  2:2818005/1‑140 (MQ=255)
                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTGTAAATACTACAAATTTTTAAAATAAGGGGAAAAAATCCCATTTACTTGCAAAATAtt                                                               >  2:384362/1‑140 (MQ=255)
                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTACTACCAATTTGTAAAATAAGGGAAAAACATCCCATTTAATTCCAAAATAtt                                                               >  2:2797176/1‑140 (MQ=255)
                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTGTTTTTTGTAAATACTACAAATTTTAAAAATAAGGGAAAAAAAAGACAATTAATTACAAAATAtt                                                               >  2:1524849/1‑140 (MQ=255)
                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTCTTTTTTATGTAATTACTAAAAATTTGTAAAATACGGGAAAAACATCCCATTTAGTTCCAAAAtttt                                                               >  2:2026476/1‑140 (MQ=255)
                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAATACAAAATTGAAAAAAACGGGAAAAACATCCCATTTAATTCAAAAAAAtt                                                               >  2:1687221/1‑140 (MQ=255)
                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTAGTTCTTTTTTATGTGATTACTACAAATTTGAAAAATAAGTGACAAACATACCAATTCAGTACAAAatata                                                               >  2:2639383/1‑139 (MQ=255)
                                                                     aaTTTTTAAATTTATCACGATTGAAACAAAGTAAAACAAAAAAGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGa                                                            <  2:779717/140‑1 (MQ=255)
                                                                       tttttAAATTTATACCTATTTAAAAAACTTAAAACAAAAACGCCTGGGAAACCGGGATTTTTTTTGTTCTTTTTTATGTAATTACTACATATTTGAAAAATTGGGGATTAAGATCACATTTAATTCCTAAATAttgtatg                                                          <  2:1363073/140‑6 (MQ=255)
                                                                        ttttAAATTAATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  1:266976/140‑1 (MQ=255)
                                                                        ttatAAATTTATACCGATTCAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  1:2134997/137‑1 (MQ=255)
                                                                                  ataCCGATTTAAACAACGAAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTCCAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                               <  2:486253/140‑1 (MQ=255)
                                                                                         ttGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGTTCTGTTTTTTGTAATTACTACAAAATTGAAAAATACGGGGAAAAAATGCCAGTTTCTTCCCAAAGATTTGATTGTAATTTTTTGTAGGtga                                        >  2:885271/1‑138 (MQ=255)
                                                                                            aaacaaacAACAACACAAACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATACCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:1832056/140‑1 (MQ=255)
                                                                                              aCAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAttt                                   <  2:1146771/140‑1 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTTTTTTTTTTTTTATTTATAAAAAATTTAAAAAAAAAGGGAAAAAAAACCAGTTTATTACAAAAAATTTTTTTGTAATTTTTTTTTGGTGAAAttttttt                               >  1:652160/1‑136 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACTAAAAAATTTAAAAATAAGGGTAAAAAAAACCATTTAGTTACAAAATATTTGTTGGTAATTTTATGTTTGTAAAATTTTTAt                               >  2:1410815/1‑140 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACTACAAATTTTAAAAATAAGGGAAAAACATCCCATTTACTTGCAAAATATTTTATGGTAATTTTATGTAGGTAAAATTTGTAt                               >  2:1983105/1‑140 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTGTTTTATTTTTTATGTAATTATGAAAAAATTTAAAAATAAGGGAAAAACATACCCTTTACTTCCAAAATATTTTATGTTAATTTTATTTAGTTAAAAttttttt                               >  2:2464142/1‑136 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTTGTTTTTTATGTAATTACTAAAAATTTGTAAAATAAGGGAAAAACATCCCCTTTTATTCCCAAATATTTGTTTGTAATTTTATGTATGTGAAAttgtgtg                               >  2:1579105/1‑135 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAATTAATACAAATTTGTAAAATAAGGGAAAAACATACCAATTAATTAAAAAAAATTTTATGGTAATTTTATTTAGGTaaaaataagta                               >  2:495376/1‑133 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAATTAATACAAAATTTTAAAATAAGGGAAAAAAAACCCAATTTCTTCCAAAATATTTTATGGTAATTTTATTTAGGTAAAATTTGttt                               >  2:1455866/1‑138 (MQ=255)
                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAAATAATAAAAAATTTAAAAATAAGGGAAAAAAATACCATTTAATTACAAAAAATTTTATGGTAATTTTATTTAGGTAAAATTTTTAt                               >  2:1481878/1‑140 (MQ=255)
                                                                                                   caaacaCAAAAACGCATGGGAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                              <  1:2673038/135‑1 (MQ=255)
                                                                                                      aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2797176/140‑1 (MQ=255)
                                                                                                      aaaaaaaaACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1078721/140‑1 (MQ=255)
                                                                                                      aaaaaaaaACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1558349/140‑1 (MQ=255)
                                                                                                      aaaaaaaaACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:14805/140‑1 (MQ=255)
                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1324972/140‑1 (MQ=255)
                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1850530/140‑1 (MQ=255)
                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:1691981/140‑1 (MQ=255)
                                                                                                      aaaCAAAAACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:127825/140‑1 (MQ=255)
                                                                                                       aaCAAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1850234/140‑1 (MQ=255)
                                                                                                                 gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:19144/140‑1 (MQ=255)
                                                                                                                 gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:384362/140‑1 (MQ=255)
                                                                                                                 gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:2114675/140‑1 (MQ=255)
                                                                                                                 gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  1:1481878/140‑1 (MQ=255)
                                                                                                                 cccTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:188532/139‑1 (MQ=255)
                                                                                                                      tgAAAGCGTGCGTTTTTTTTTTCTTTTTTATTTAATTAATACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt            <  1:1687221/139‑1 (MQ=255)
                                                                                                                      gcaaaCCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACGTCCAAACTATTTGAGGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt            <  1:2279903/138‑1 (MQ=255)
                                                                                                                          aCCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaa        <  1:327750/140‑1 (MQ=255)
                                                                                                                           ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa       <  1:995597/140‑1 (MQ=255)
                                                                                                                           ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa       <  1:1613705/140‑1 (MQ=255)
                                                                                                                           ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa       <  1:1065755/140‑1 (MQ=255)
                                                                                                                                cGTTTTTTTGTTCTTTTTTATGTAATTACTACATATTTGAAAAATACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:2753451/140‑1 (MQ=255)
                                                                                                                                cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAACAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:1496381/140‑1 (MQ=255)
                                                                                                                                cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  1:503598/140‑1 (MQ=255)
                                                                                                                                cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:681692/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                
GCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991097‑2991363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: