Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,5670CT32.5% 428.7 / 308.8 305L278L (CTG→CTAEO946_RS16995IS3 family transposase
Reads supporting (aligned to +/- strand):  ref base C (99/107);  new base T (55/44);  total (154/151)
Fisher's exact test for biased strand distribution p-value = 2.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 305 total aligned reads displayed.

GAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATG  >  NZ_CP034943/3285430‑3285702
                                                                                                                                         |                                                                                                                                       
gAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAg                                                                                                                                       <  1:518974/140‑1 (MQ=32)
    cATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGa                                                                                                                                   >  2:605946/1‑140 (MQ=32)
          cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                             <  1:1176372/140‑1 (MQ=32)
          cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                             <  2:2751934/140‑1 (MQ=32)
          cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                             <  2:1097079/140‑1 (MQ=32)
          cAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                             <  1:1477704/140‑1 (MQ=32)
           aGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCGTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                             >  1:1345537/1‑139 (MQ=17)
           aGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGt                                                                                                                             >  1:737325/1‑139 (MQ=32)
                gCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCt                                                                                                                       <  2:1764971/140‑1 (MQ=34)
                   tCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAg                                                                                                                    >  1:386692/1‑140 (MQ=34)
                     ccTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                                                                                                  <  2:419285/140‑1 (MQ=34)
                       tttGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACaa                                                                                                                <  1:1194610/140‑1 (MQ=34)
                            gggACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCt                                                                                                           <  1:62949/140‑1 (MQ=34)
                            gggACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCt                                                                                                           <  1:2095261/140‑1 (MQ=34)
                                cATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACTCAGATCTCAGTCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAATTCTgtgt                                                                                                       >  2:2511648/1‑140 (MQ=255)
                                 aTGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                                                                                                      <  2:1714644/140‑1 (MQ=34)
                                     tCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGc                                                                                                  <  2:1252847/140‑1 (MQ=34)
                                              tGCGTTGTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATCCACAGACCCTTGGTCACTATGTAACACGCAGTTCTCAGGCCGTGTTGGCAGCTGATTAAGTATGTCTAAGACCAAGTCTGGGTCCTGCTTATCCCCa                                                                                         >  2:117377/1‑140 (MQ=14)
                                                  ttttGTGTTAACAGCTTTCTGATACTCTTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCg                                                                                     <  2:1596973/140‑1 (MQ=11)
                                                   tttGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt                                                                                    >  1:1384168/1‑140 (MQ=255)
                                                   tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt                                                                                    >  2:2276859/1‑140 (MQ=255)
                                                   tttGTGTTAACAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTAAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGt                                                                                    >  1:2025681/1‑140 (MQ=11)
                                                     tgtgTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTaa                                                                                  >  2:1892806/1‑140 (MQ=255)
                                                          tAATAGCTTTCTTATAATCGTAAGATGTATTCAGAGAACCTTGGTCACTATTTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTTATTATGTCTAAGACAAAGTGTGTGTCCTGATTATCCCCAATCGTAAAAGCa                                                                             <  1:2511648/140‑1 (MQ=14)
                                                          tAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                             <  1:2423290/140‑1 (MQ=255)
                                                          tAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCa                                                                             >  1:2114785/1‑140 (MQ=255)
                                                            aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt                                                                           <  1:2404298/140‑1 (MQ=255)
                                                            aTAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGGCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAAt                                                                           <  1:2339242/140‑1 (MQ=255)
                                                                 tttCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                      <  2:1016522/140‑1 (MQ=40)
                                                                 tttCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                      <  1:2786248/140‑1 (MQ=40)
                                                                   tCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                    <  2:2304037/140‑1 (MQ=40)
                                                                   tCTGATACTCGTAAGATGTATACACAGAACATTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                    <  1:1879559/140‑1 (MQ=255)
                                                                      gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                 <  2:855311/140‑1 (MQ=255)
                                                                      gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                 <  2:779795/140‑1 (MQ=255)
                                                                      gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                 <  1:2319051/140‑1 (MQ=255)
                                                                      gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                    <  2:12254/137‑1 (MQ=40)
                                                                      gATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                 <  2:2246500/140‑1 (MQ=40)
                                                                          cTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGt                                                             >  1:2003147/1‑140 (MQ=255)
                                                                           tCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGta                                                            <  1:2216127/140‑1 (MQ=40)
                                                                           tCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGta                                                            <  2:742277/140‑1 (MQ=40)
                                                                             gTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtata                                                          >  1:272261/1‑140 (MQ=255)
                                                                             gTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtata                                                          >  1:2272419/1‑140 (MQ=40)
                                                                                   tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                    <  2:450619/140‑1 (MQ=255)
                                                                                   tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                    <  2:1792897/140‑1 (MQ=255)
                                                                                   tGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTa                                                    <  2:1625105/140‑1 (MQ=255)
                                                                                     tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTaa                                                   <  1:1707165/139‑1 (MQ=255)
                                                                                     tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTaa                                                   >  2:1320547/1‑139 (MQ=255)
                                                                                     tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTaa                                                   <  1:1320547/139‑1 (MQ=255)
                                                                                     tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAat                                                  >  2:2347604/1‑140 (MQ=255)
                                                                                     tataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAat                                                  >  1:1757477/1‑140 (MQ=255)
                                                                                      ataCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAata                                                 <  1:2626017/140‑1 (MQ=255)
                                                                                       taCACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGAAATCACTTCTCCATTGTATAAATCTAatat                                                >  1:1545678/1‑140 (MQ=11)
                                                                                            aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTc                                                                                                      <  1:302284/81‑1 (MQ=33)
                                                                                            aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                           >  2:2532337/1‑140 (MQ=255)
                                                                                            aGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTgg                                           >  1:255904/1‑140 (MQ=255)
                                                                                              aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                         <  2:2777526/140‑1 (MQ=255)
                                                                                              aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                    <  1:1457968/113‑1 (MQ=255)
                                                                                              aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTtct                                                                    >  2:1458785/1‑113 (MQ=255)
                                                                                              aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGta                                                            >  2:2469437/1‑121 (MQ=40)
                                                                                              aaCCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGaa                                         <  1:1015658/140‑1 (MQ=255)
                                                                                                   tGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTa                                    >  2:311431/1‑140 (MQ=255)
                                                                                                        aCTATGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtataa                                                         <  1:1588506/114‑2 (MQ=255)
                                                                                                        aCTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAAtt                               >  1:316734/1‑140 (MQ=255)
                                                                                                          tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGt                             >  2:2183073/1‑140 (MQ=255)
                                                                                                          tATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATACCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAACCTACAAtttt                             >  2:1546834/1‑138 (MQ=255)
                                                                                                            tGTAACACGCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGttt                           <  1:921955/140‑1 (MQ=255)
                                                                                                                acacGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGt                       <  1:1288746/140‑1 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGtat                                                           >  2:1587725/1‑100 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTCATATACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  2:2423871/1‑140 (MQ=11)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  2:2106603/1‑140 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  2:1530406/1‑140 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATCTACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  1:823471/1‑140 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  1:2163652/1‑140 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  2:1325601/1‑140 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  1:1238810/1‑140 (MQ=255)
                                                                                                                    gCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCAt                   >  2:2429782/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCTTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  2:2074283/1‑140 (MQ=11)
                                                                                                                      aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCa                                                                 >  1:575532/1‑92 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  2:2812491/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGAc                                                                                                                  <  2:672466/43‑1 (MQ=38)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCa                                                                         <  1:1123729/84‑1 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  2:2005638/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  2:1991013/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  2:2650310/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  1:1640102/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  2:1358055/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  1:1371098/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATaa                 >  2:480290/1‑140 (MQ=255)
                                                                                                                      aGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGGCTAAGAc                                                                                                                  >  1:672466/1‑43 (MQ=255)
                                                                                                                        ttCTCAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATCTACTGGAAAGGTACACTTGTTTCTGTCCATAAgc               >  2:1686298/1‑139 (MQ=11)
                                                                                                                          ctcAGGCAGTGTTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCa             <  1:100134/140‑1 (MQ=255)
                                                                                                                            cAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCaaa           <  2:906065/140‑1 (MQ=255)
                                                                                                                            cAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCaaa           <  2:128481/140‑1 (MQ=255)
                                                                                                                             aGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACtt                                                                      >  2:540229/1‑80 (MQ=255)
                                                                                                                                  gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGaa                                         <  1:430273/104‑1 (MQ=11)
                                                                                                                                  gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAgg                                      >  2:1849292/1‑107 (MQ=11)
                                                                                                                                  gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg     >  2:348507/1‑140 (MQ=255)
                                                                                                                                  gtgtTGGTAGCTGATGAAGTGTGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg     >  1:457533/1‑140 (MQ=255)
                                                                                                                                  gtgtTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAtgtg     >  2:627486/1‑140 (MQ=255)
                                                                                                                                     ttGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATg  <  1:1586980/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GAGGCATTATCAGCGGGCGTCCCTTTGCGGGACATGCTCATGGTAATGCCTTTTGTGTTAATAGCTTTCTGATACTCGTAAGATGTATACACAGAACCTTGGTCACTATGTAACACGCAGTTCTCAGGCAGTGTTGGCAGCTGATTAAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATG  >  NZ_CP034943/3285430‑3285702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: