Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9700GT27.7% 251.6 / 39.7 156Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (36/76);  new base T (41/2);  total (78/78)
Fisher's exact test for biased strand distribution p-value = 9.34e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 160 total aligned reads displayed.

CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108
                                                                                                                                            |                                                                                                                                           
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTg                                                                                                                                             >  2:2691882/1‑140 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTg                                                                                                                                             >  2:1552605/1‑140 (MQ=255)
ctATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAAATTTTTg                                                                                                                                             >  2:451901/1‑140 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCa                                                                                                                                           <  1:370402/140‑1 (MQ=255)
   ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATAAATTTTTTCaa                                                                                                                                          >  1:597355/1‑140 (MQ=255)
       aatggAACCGGCAGCCTAAAACAAGACCTCCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCCTTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAata                                                                                                                                      <  2:1716988/137‑1 (MQ=255)
       aatggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCAATTTTTAATGGCGACCACAACGGATTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAata                                                                                                                                      <  2:1889361/137‑1 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCTT‑TTAACTTCATTTTTTTCAAAatat                                                                                                                                     >  1:906475/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACATTAAATTTTTGCAAAaaat                                                                                                                                     >  2:795308/1‑140 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATATTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑ATACAATCATTTTTTTCAAAatat                                                                                                                                     >  1:2276490/1‑140 (MQ=255)
           gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTAAATTCATTTTTTTCAAAATATTGc                                                                                                                                  >  2:2796952/1‑140 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGGTc                                                                                                                                >  2:634051/1‑140 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTTCAATCATTTTTTTCAAAATAtttgtc                                                                                                                                >  1:841314/1‑136 (MQ=255)
             aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATGATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAACTTAATTTTTTGCAAAATATTGCTc                                                                                                                                >  2:872531/1‑140 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                             <  1:1555640/140‑1 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                             <  1:2196174/140‑1 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                             <  2:47197/140‑1 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                             >  2:1193552/1‑140 (MQ=255)
                           aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTAAAAATATTGCTCACATGGTTTTTCAc                                                                                                                  <  2:306538/140‑1 (MQ=255)
                           aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                  <  2:445078/140‑1 (MQ=255)
                           aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                  <  1:2238657/140‑1 (MQ=255)
                            aaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACCTTCATTTTTTTCAAAATATTTCTCCCATGGTTTTTCACg                                                                                                                 >  2:1330552/1‑140 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                          <  1:2691882/140‑1 (MQ=255)
                                    gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAATATTGCTCACAAGGTTTTTGACGGttttgtg                                                                                                         >  1:50212/1‑137 (MQ=255)
                                     cccAGTAAGAGGCAAGTCTCTTATTATACGATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACCGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                        <  2:80080/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                        <  1:2480130/140‑1 (MQ=255)
                                         gTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCCAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTgt                                                                                                    >  2:834758/1‑139 (MQ=255)
                                              aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                                               <  2:2265670/140‑1 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACa                                                                                              <  1:521730/140‑1 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATAATTTTTTTGAAAATATTTCTCAAATGGTTTTTGACGGTTTTGTCCCTTGTAAACa                                                                                              >  1:85103/1‑140 (MQ=255)
                                               ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTAAATTCATTTTTTTCAAAAAATTTCTAACATGGTTTTTCACCGTTTTATAAATGATaaaaa                                                                                              >  1:157743/1‑138 (MQ=255)
                                                gCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACaa                                                                                             <  2:1765521/140‑1 (MQ=255)
                                                 cAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAAt                                                                                            <  1:1913825/140‑1 (MQ=255)
                                                  aaGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATg                                                                                           >  2:1202630/1‑140 (MQ=255)
                                                   gacagctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                          <  1:662424/135‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                          <  1:2537301/140‑1 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTTCAATCATTTTTTGCAAAAAATTTCTCAACAGGTTTTTAACGGTTTTATAAATTATAAACAAAGAt                                                                                         >  1:2835245/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATAATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                         >  2:2090855/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTAAATTAATTTTTTTCAAAAAATTTCTAACATGGTTTTTAACGGTTTTATCACTGATAAAAAATTAt                                                                                         >  1:2463311/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACATTAATTTTTTTCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTCAATGATaaaaaaatat                                                                                         >  1:1367483/1‑136 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAAATTAAATTTTTTAAAAATATTTCTCAAAAGGTTTTTAAAGGTTTTAAGACAGATAAAAAAAGAt                                                                                         >  1:1785570/1‑140 (MQ=255)
                                                        ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑Ac                                                                                    <  1:2525697/140‑1 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTTCAAAATAATTCTCACAATGTTTTTCAAGGTTTTATCACTGATAAAAAATGATTA‑ACCAATaac                                                                             >  1:1886083/1‑138 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATCATTTTTTGCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATAACAGATAAAAAAAGATTA‑AACCATAAcgc                                                                           >  1:1827021/1‑140 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTAACATATAAAAAAAGATTC‑ACCaaatacgc                                                                           >  1:2612583/1‑134 (MQ=255)
                                                                 cTATCTAATTTCTACCCAGCCATTTTTATTGGCGACAACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTGCAAAATATTTGGAACATGGTTTTTCACCGTTTTCTAAGTGATAAACAATGATTC‑ACAAATACcgc                                                                           >  2:1007080/1‑140 (MQ=255)
                                                                  tATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTCTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACcgcg                                                                          <  1:1286608/140‑1 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTAAATAAATTTTTT‑CAAAATAATTCTCACAAGGTTTTTGACGGTTTTCTAACTGATAAACAATGATTA‑ACCAAAACCGCgg                                                                         >  1:1616302/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAATAATTCTCACAAGGTTTTTCAAGGTTTTATAACTGATAAACAAAGATTA‑AACAATAAAGCgg                                                                         >  2:2286069/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAAAATTTCTCACCTGGTTTTTCACGGGTTTCTCAATGATAAAAAAAGATTA‑ACCAAAAACGCgg                                                                         >  1:507216/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTCACTGATAAAAAATTATTA‑AACAAAAACGCgg                                                                         >  2:1869033/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAAATATTC‑ACCAATAACGCgg                                                                         >  2:2128201/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAAAGGTTTTATCACTTATAAACAATGTTTC‑ACCAAAACAGCgg                                                                         >  2:1626806/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCAATTTTTTCAAAATAATGCTCACATGGTTTTTAACGGTTTTCTAACTGATAAACAATGATTC‑ACCAATAACGCgg                                                                         >  2:1004099/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTTCAAAAAATTTCTAAAATGGTTTTTAAACGTTTTATCACTGATAAAAAAATATTC‑AACAAAACCCCgg                                                                         >  1:146432/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTGAAAAATATTGCTCACAAGGTTTTTAACGGTTTTCTAACTTATAAAAAATGATTA‑AACAATaaaacgg                                                                         >  1:1326627/1‑134 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTAAATTAATTTTTTTCAAAAAAATTCTCACAAGGTTTTTAACCGTTTTATCACAGAAAAACAATGATTA‑ACCAAAAcatgta                                                                         >  2:662424/1‑135 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAAATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAAAGGTTTTATCACTTCTAAACAAAGATTC‑ACCAATACCGCgg                                                                         >  1:2610432/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAAAATCATTTTTTTCAAAAAATTTATAACAAGGTTTTTAACGGTTTTCTCAATGATAAAAAATTATTC‑ACCAAAAAcgccg                                                                         >  1:145622/1‑138 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCggg                                                                        <  1:2423479/140‑2 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCAATTTTTTCAAAATATTGCGCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTC‑ACCAATACCGCGGt                                                                        >  2:1869339/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACAGTCATTTTTTGCAAAATATTGCGCAAATGGTTTTTGACGGTTTTGACACATATAAAAAATGATTC‑ACCCAAACCCCggg                                                                        >  1:1048848/1‑139 (MQ=255)
                                                                     ctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTTCAATCATTTTTTGGAAAAAATTTCTCACATGGTTTTTAACGGTTTTGTCACAGATAAAAAAAGATTA‑AACAATAACGCGGtt                                                                       >  1:1366122/1‑140 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTTCAATGATTTTTTTGAAAAAATTTCTAACATGGTTTTTGACCGTTTTGTCACAGATAAAAAAATAATC‑AACAATACCGCggttggtt                                                                   >  1:341420/1‑140 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTTCATTCATTTTTTTCAAAATATTGGTCACATGGTTTTTAACGGTTTTGTCACTGATAAACAAAGATTC‑ACCAATACCGCGGTTGCtttttgg                                                              >  1:1714132/1‑138 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TGAGAATGAAATTTTTAAAAAAAATTCTAACAATGTTTTTAAAGGCTTTAAAACAGATAAAAAATTATTA‑AACAAAAAAGCCGTAGCtttttaa                                                              >  1:1237641/1‑138 (MQ=255)
                                                                                   gCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTg                                                         <  1:1869082/140‑1 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  2:1831613/140‑1 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  1:2829527/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                    <  1:1167189/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                    <  1:1626806/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                    <  1:1159070/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                    <  1:720844/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                    <  1:1921441/140‑1 (MQ=255)
                                                                                             aTGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTg                                               <  2:829612/140‑1 (MQ=255)
                                                                                              tGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                              <  1:1202630/140‑1 (MQ=255)
                                                                                              tGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTGTTCCGTCTGCAAGCATCTgc                                              <  1:2368345/140‑1 (MQ=255)
                                                                                              tGGCGACCACAACGGCGTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                              <  1:1642175/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  2:297611/140‑1 (MQ=255)
                                                                                                gCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcag                                            <  2:149798/140‑1 (MQ=255)
                                                                                                  gACCACCACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcagca                                          <  2:507216/140‑1 (MQ=255)
                                                                                                   aCCACAACGGCTTGCGTCCGGTCGT‑TTTCATTAATTTTTTTCAAAATAATTCTCACATGGTTTTTAAACGTTTTATGACAGATAAAAAAATATTA‑AACAAAACCGCGGTTTCTTTTTAAGTCTGCAAACATATgaagcaa                                         >  1:1061060/1‑139 (MQ=255)
                                                                                                   aCCACAACGGCTTGCGTCCGGTCGT‑TTACCTTCATTTTTTGCAAAAAATTTCTCACATGGTTTTTCACGGTTTTATGACTGATAAACAATGTTTA‑ACCAAAACCGCGGTTGGTTTTTCCGTGTGCAAGCATCTGCAGCAc                                         >  1:2083502/1‑140 (MQ=255)
                                                                                                   aCCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCCCATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATAACGCGGTTGCTTTTTCCGTCGGCAAGCATCTGCAGCAc                                         >  2:608918/1‑140 (MQ=255)
                                                                                                   aCCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCAc                                         >  2:2708935/1‑140 (MQ=255)
                                                                                                               tGCGTCCGGTCGT‑TTTCATTGAATTTTTGCAAAAAATTGCTCACATGGTTTTTCACCGTTTTATCACTTATAAAAAAATATTCAACCAAAACC‑CGGTTGGGTTTTGCGTGTGCAAGCATCTGCAGCACGTCACATTcccc                             >  1:519785/1‑139 (MQ=255)
                                                                                                               tGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccg                             <  2:2636208/140‑1 (MQ=255)
                                                                                                                 cGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                           <  2:2612583/140‑1 (MQ=255)
                                                                                                                    ccGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                        <  2:1827021/140‑1 (MQ=255)
                                                                                                                    ccGGTCGT‑TTACATTCATTTTTTGCAAAATAGTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                        <  2:2364504/140‑1 (MQ=255)
                                                                                                                      ggTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                      <  2:1543144/140‑1 (MQ=255)
                                                                                                                      ggTCGT‑GTACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCAACTGCAGCACTTCACATTCCCGCCGAGtt                      >  2:505060/1‑140 (MQ=255)
                                                                                                                         ctt‑ttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:2458327/138‑1 (MQ=255)
                                                                                                                         cGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTaaa                   <  2:2560412/140‑1 (MQ=255)
                                                                                                                         cGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTGGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:451901/140‑1 (MQ=255)
                                                                                                                                         tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg    <  1:2490110/140‑1 (MQ=255)
                                                                                                                                           tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:2461752/140‑1 (MQ=255)
                                                                                                                                           tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:2286069/140‑1 (MQ=255)
                                                                                                                                           tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  >  1:243705/1‑140 (MQ=255)
                                                                                                                                           tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:1515199/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑