Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9800GT24.7% 300.7 / 41.1 162S202R (AGC→AGA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (32/90);  new base T (40/0);  total (72/90)
Fisher's exact test for biased strand distribution p-value = 1.87e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 163 total aligned reads displayed.

GAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAT  >  NZ_CP034943/3446842‑3447119
                                                                                                                                           |                                                                                                                                            
  aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATAAATTTTTTGCAAAAAAATGCta                                                                                                                                           >  1:2560412/1‑139 (MQ=255)
  aaCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATGATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAACTTAATTTTTTGCAAAATATTGCTc                                                                                                                                           >  2:872531/1‑140 (MQ=255)
     ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                                        <  2:47197/140‑1 (MQ=255)
     ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                                        <  2:700962/140‑1 (MQ=255)
     ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                                        <  1:2196174/140‑1 (MQ=255)
     ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                                        <  1:1555640/140‑1 (MQ=255)
     ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                                        >  2:1193552/1‑140 (MQ=255)
                aaaaGACCTTCCCAGTAAAAGGCAAGTCTATTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCACAATATTGCTCACATGGTTTTTCAc                                                                                                                             <  1:2239482/140‑1 (MQ=255)
                aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCCCAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                             <  1:564647/140‑1 (MQ=255)
                 aaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACCTTCATTTTTTTCAAAATATTTCTCCCATGGTTTTTCACg                                                                                                                            >  2:1330552/1‑140 (MQ=255)
                 aaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACg                                                                                                                            <  2:416620/140‑1 (MQ=255)
                         gCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAATATTGCTCACAAGGTTTTTGACGGttttgtg                                                                                                                    >  1:50212/1‑137 (MQ=255)
                          cccAGTAAGAGGCAAGTCTCTTATTATACGATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACCGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                   <  2:80080/140‑1 (MQ=255)
                          cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                                   <  2:1626357/140‑1 (MQ=255)
                                   aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                                                          <  2:2265670/140‑1 (MQ=255)
                                   aGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAAc                                                                                                          <  2:2266295/140‑1 (MQ=255)
                                    ggCACGTCTGTTGTTATACTAGCTCATTTCTACCCAGCCATTTTTAATGGCGCCCACAACGGCTTGCGTCCGGTCGT‑TGACATTCATTTTTTGCAATATATTGCTAACATGGGTTTTAAAGGTTTTCTAACAGATAAACa                                                                                                         >  2:286008/1‑140 (MQ=255)
                                    ggCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAAATTAAATTTTTGCAAAATATTTCTCACAATGTTTTTAACGGTTTTATCAATGATaaaaa                                                                                                         >  1:191077/1‑138 (MQ=255)
                                     gCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACaa                                                                                                        <  2:1765521/140‑1 (MQ=255)
                                      cAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAAt                                                                                                       <  1:1913825/140‑1 (MQ=255)
                                       aaGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATg                                                                                                      >  2:1202630/1‑140 (MQ=255)
                                        gacagctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                     <  1:662424/135‑1 (MQ=255)
                                        aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                                     <  1:512551/140‑1 (MQ=255)
                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTTCAATCATTTTTTGCAAAAAATTTCTCAACAGGTTTTTAACGGTTTTATAAATTATAAACAAAGAt                                                                                                    >  1:2835245/1‑140 (MQ=255)
                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTTCTAACAAGGTTTTTAACGGTTTTATCACTTATAAAAAATTAt                                                                                                    >  2:512551/1‑140 (MQ=255)
                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATAATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                                    >  2:2090855/1‑140 (MQ=255)
                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATAAATTTTTTCAAAAAATTTATCACAATGTTTTTAAAGGTTTTGTAACTGATaaaaaaatat                                                                                                    >  1:248528/1‑136 (MQ=255)
                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTAAATTAATTTTTTTCAAAAAATTTCTAACATGGTTTTTAACGGTTTTATCACTGATAAAAAATTAt                                                                                                    >  1:2463311/1‑140 (MQ=255)
                                         gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACATTAATTTTTTTCAAAATATTTCTCACATTGTTTTTCACGGTTTTCTCAATGATaaaaaaatat                                                                                                    >  1:1367483/1‑136 (MQ=255)
                                             ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACCACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑Ac                                                                                               <  1:1820947/140‑1 (MQ=255)
                                             ctTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑Ac                                                                                               <  1:2525697/140‑1 (MQ=255)
                                                      cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATCATTTTTTGCAAAAAATTTCTCACATGGTTTTTAACGGTTTTATAACAGATAAAAAAAGATTA‑AACCATAAcgc                                                                                      >  1:1827021/1‑140 (MQ=255)
                                                      cTATCTAATTTCTACCCAGCCATTTTTATTGGCGACAACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTGCAAAATATTTGGAACATGGTTTTTCACCGTTTTCTAAGTGATAAACAATGATTC‑ACAAATACcgc                                                                                      >  2:1007080/1‑140 (MQ=255)
                                                       tATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTCTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACcgcg                                                                                     <  1:1286608/140‑1 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTAAATAAATTTTTT‑CAAAATAATTCTCACAAGGTTTTTGACGGTTTTCTAACTGATAAACAATGATTA‑ACCAAAACCGCgg                                                                                    >  1:1616302/1‑140 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAATAATTCTCACAAGGTTTTTCAAGGTTTTATAACTGATAAACAAAGATTA‑AACAATAAAGCgg                                                                                    >  2:2286069/1‑140 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTAAAGGTTTTATCACTTATAAACAATGTTTC‑ACCAAAACAGCgg                                                                                    >  2:1626806/1‑140 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTTCAAAAAATTTCTAAAATGGTTTTTAAACGTTTTATCACTGATAAAAAAATATTC‑AACAAAACCCCgg                                                                                    >  1:146432/1‑140 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTCAAGGTTTTATCACTGATAAAAAATGATTC‑ACCAATAACGCgg                                                                                    >  2:1869082/1‑140 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTGCAAAAAATTGATCACATGGTTTTTAACGGTTTTCTCACATATAAAAAATGATTC‑ACCAATaaaccgg                                                                                    >  2:1539276/1‑134 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTGAAAAATATTGCTCACAAGGTTTTTAACGGTTTTCTAACTTATAAAAAATGATTA‑AACAATaaaacgg                                                                                    >  1:1326627/1‑134 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTAAATTAATTTTTTGCAAAATATTTCTCACAAGGTTTTTCAAGGTTTTCTAACTGAAAAAAAATGATTC‑ACCCATAcccccg                                                                                    >  2:2594536/1‑136 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAAATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAAAGGTTTTATCACTTCTAAACAAAGATTC‑ACCAATACCGCgg                                                                                    >  1:2610432/1‑140 (MQ=255)
                                                        aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTAAAATCATTTTTTTCAAAAAATTTATAACAAGGTTTTTAACGGTTTTCTCAATGATAAAAAATTATTC‑ACCAAAAAcgccg                                                                                    >  1:145622/1‑138 (MQ=255)
                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTAGATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTATCACTGCTAAACAAAGATTC‑ACCAATACACCGGt                                                                                   >  2:1476242/1‑140 (MQ=255)
                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCAAATATAAAAAAAGAATA‑AACAATAAAGCGGt                                                                                   >  2:2447731/1‑140 (MQ=255)
                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCggg                                                                                   <  1:2423479/140‑2 (MQ=255)
                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCggg                                                                                   <  1:1976269/140‑2 (MQ=255)
                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCAATTTTTTCAAAATATTGCGCACATGGTTTTTCACGGTTTTCTCACTGATAAAAAATGATTC‑ACCAATACCGCGGt                                                                                   >  2:1869339/1‑140 (MQ=255)
                                                         tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑GTACAGTCATTTTTTGCAAAATATTGCGCAAATGGTTTTTGACGGTTTTGACACATATAAAAAATGATTC‑ACCCAAACCCCggg                                                                                   >  1:1048848/1‑139 (MQ=255)
                                                          ctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAATAATTCTCACATGGTTTTTCACGGTTTTCTCAATGATAAAAAAAGATTA‑ACCAATACCGCGGtt                                                                                  >  1:2697631/1‑140 (MQ=255)
                                                              tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTAATTTTTTGCAAAATATTGCTCACATGGTTTTTAACGGTTTTCTCAATTATAAACAATGATTA‑ACCAATAAAGCGGTTGCtt                                                                              >  2:2659134/1‑140 (MQ=255)
                                                              tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACAATAATTTTTTGCAAAAAAATTGTCACATGGTTTTTAACGGTTTTATCACTGATAAAAAAAGATTA‑ACCAAAACCCCCGTTGCtt                                                                              >  1:297611/1‑140 (MQ=255)
                                                                   aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTTCATTCATTTTTTTCAAAATATTGGTCACATGGTTTTTAACGGTTTTGTCACTGATAAACAAAGATTC‑ACCAATACCGCGGTTGCtttttgg                                                                         >  1:1714132/1‑138 (MQ=255)
                                                                   aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TGAGAATGAAATTTTTAAAAAAAATTCTAACAATGTTTTTAAAGGCTTTAAAACAGATAAAAAATTATTA‑AACAAAAAAGCCGTAGCtttttaa                                                                         >  1:1237641/1‑138 (MQ=255)
                                                                       aGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCt                                                                     >  2:640560/1‑140 (MQ=255)
                                                                        gCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTg                                                                    <  1:1869082/140‑1 (MQ=255)
                                                                          cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                                  <  2:1831613/140‑1 (MQ=255)
                                                                             ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                               <  1:1167189/140‑1 (MQ=255)
                                                                             ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                               <  1:2128201/140‑1 (MQ=255)
                                                                             ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                               <  1:1626806/140‑1 (MQ=255)
                                                                             ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                               <  1:1159070/140‑1 (MQ=255)
                                                                                  aTGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTg                                                          <  2:829612/140‑1 (MQ=255)
                                                                                   tGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                                         <  1:1202630/140‑1 (MQ=255)
                                                                                   tGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTGTTCCGTCTGCAAGCATCTgc                                                         <  1:2368345/140‑1 (MQ=255)
                                                                                     gCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcag                                                       <  2:297611/140‑1 (MQ=255)
                                                                                       gACCACCACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                     <  2:507216/140‑1 (MQ=255)
                                                                                       gACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                     <  1:1367112/140‑1 (MQ=255)
                                                                                       gACCACAACGGCTTGCGTCCGGTAGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                                     <  1:952232/140‑1 (MQ=255)
                                                                                        aCCACAACGGCTTGCGTCCGGTCGT‑TTTCATTAATTTTTTTCAAAATAATTCTCACATGGTTTTTAAACGTTTTATGACAGATAAAAAAATATTA‑AACAAAACCGCGGTTTCTTTTTAAGTCTGCAAACATATgaagcaa                                                    >  1:1061060/1‑139 (MQ=255)
                                                                                        aCCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTTCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCAc                                                    >  2:2787817/1‑140 (MQ=255)
                                                                                        aCCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCCCATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATAACGCGGTTGCTTTTTCCGTCGGCAAGCATCTGCAGCAc                                                    >  2:608918/1‑140 (MQ=255)
                                                                                        aCCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCAc                                                    >  2:2708935/1‑140 (MQ=255)
                                                                                                 gCTTGCGTCCGGTCGT‑TTACATTAATTTTTTTGAAAAAATTTCTCACAAGGTTTTTAACGGTTTTGTCACTGATAAAAAATGTTTG‑ACCAAAAAAGCGGGTTGTTTTTTCGTGTGCAATCAAATGGAGCACATaacaata                                           >  1:1651441/1‑137 (MQ=255)
                                                                                                    tGCGTCCGGTCGT‑TTTCATTGAATTTTTGCAAAAAATTGCTCACATGGTTTTTCACCGTTTTATCACTTATAAAAAAATATTCAACCAAAACC‑CGGTTGGGTTTTGCGTGTGCAAGCATCTGCAGCACGTCACATTcccc                                        >  1:519785/1‑139 (MQ=255)
                                                                                                     gCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgc                                       <  2:2515550/140‑1 (MQ=255)
                                                                                                      cGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                      <  1:1585869/140‑1 (MQ=255)
                                                                                                      cGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                                      <  2:2612583/140‑1 (MQ=255)
                                                                                                         ccGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAg                                   <  2:469980/140‑1 (MQ=255)
                                                                                                           ggTCGT‑TTACATTCATTTTTTTCAAAATATTGCTCAAATGGTTTTTAACGGTTTTGTCACTGATAAACAATGATTA‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                                 >  2:179222/1‑140 (MQ=255)
                                                                                                            gTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTa                                <  1:231425/140‑1 (MQ=255)
                                                                                                              ctt‑ttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                              <  2:2458327/138‑1 (MQ=255)
                                                                                                              cGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                              <  1:442029/140‑1 (MQ=255)
                                                                                                              cGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                              <  2:85103/140‑1 (MQ=255)
                                                                                                              cGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                              <  1:865171/140‑1 (MQ=255)
                                                                                                                              tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg               <  1:7448/140‑1 (MQ=255)
                                                                                                                              tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTg               <  1:634051/140‑1 (MQ=255)
                                                                                                                              tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg               <  1:2490110/140‑1 (MQ=255)
                                                                                                                                tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCACTACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTGAAAATATGTAATGGTCTGCg             <  1:2658856/140‑1 (MQ=255)
                                                                                                                                tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             >  1:243705/1‑140 (MQ=255)
                                                                                                                                tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg             <  2:1515199/140‑1 (MQ=255)
                                                                                                                                     attatTGCTCAAATGTTTTTTCTCGGTTTTATAAATTATAAACATTGATTC‑CCCAATACCGCTGTTGGTTTTTCCGTCTGCAATCATCTTCAGCACTTCACATTCCCGCCGAGTTAAAATTTTTAATTGTCTGGGGtttt        <  2:906475/138‑1 (MQ=255)
                                                                                                                                     aaTATTGCTCAGATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:1616295/140‑1 (MQ=255)
                                                                                                                                     aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:50212/140‑1 (MQ=255)
                                                                                                                                     aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:597355/140‑1 (MQ=255)
                                                                                                                                     aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:1392107/140‑1 (MQ=255)
                                                                                                                                     aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:2659134/140‑1 (MQ=255)
                                                                                                                                     aaTATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:2140553/140‑1 (MQ=255)
                                                                                                                                     aaTATTGCTCACATGGTTTGTCACGGTTTTCTCACTGATAAACAATGATTC‑CCCCAGACCGCGGTTGCTTTTTCCGTCTGCAAGGATCTGCAGCACTTCACTGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  1:481210/140‑1 (MQ=255)
                                                                                                                                     aaTATTGCGCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGAttt        <  2:2154852/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGT‑TTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTC‑ACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCGGATTTCAGGAT  >  NZ_CP034943/3446842‑3447119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑