Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,531 A→C 5.1% intergenic (+209/‑95) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,5310AC 5.1% 1854.5 / 18.9 753intergenic (+209/‑95)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (276/405);  new base C (68/3);  total (344/409)
Fisher's exact test for biased strand distribution p-value = 1.86e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 763 total aligned reads displayed.

AGAGATCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAT  >  NZ_CP034943/112392‑112670
                                                                                                                                           |                                                                                                                                           
    aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCcgc                                                                                                                                         >  2:1851217/1‑140 (MQ=255)
    aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         <  2:7556601/140‑1 (MQ=255)
    aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         <  2:2862779/140‑1 (MQ=255)
    aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  2:5051643/1‑140 (MQ=255)
    aTCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgc                                                                                                                                         >  2:4370889/1‑140 (MQ=255)
     tCCATGAGCGCGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcggg                                                                                                                                        <  1:610858/140‑3 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAATGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:6628018/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  2:3472944/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:7115385/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:5954447/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:4277173/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:3485510/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:3245456/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  2:4778000/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:2309304/140‑1 (MQ=255)
     tCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  2:6603319/140‑1 (MQ=255)
              cGGGTTTTAGAAGTGCCCACTTAAGACCTATGAAGAACATTTTTCGAAATAATTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGTAACCGCGCGGTTTAAg                                                                                                                               <  1:263411/140‑1 (MQ=255)
                ggTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCCGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGGTTAAGCg                                                                                                                             >  2:2548696/1‑140 (MQ=255)
                   tttAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGtct                                                                                                                          <  1:7300124/140‑1 (MQ=255)
                           tGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAGGTCTGCTTTTCAATCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATg                                                                                                                  >  2:1673303/1‑140 (MQ=255)
                           tGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATg                                                                                                                  >  2:3935998/1‑140 (MQ=255)
                           tGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATg                                                                                                                  >  2:6851124/1‑140 (MQ=255)
                            gcgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGt                                                                                                                 >  1:6797173/1‑140 (MQ=255)
                            gcgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGt                                                                                                                 <  1:5288196/140‑1 (MQ=255)
                            gcgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGt                                                                                                                 >  1:4430379/1‑140 (MQ=255)
                             cgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:7531880/116‑1 (MQ=255)
                             cgcCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACcgcg                                                                                                                                        <  1:7442642/116‑1 (MQ=255)
                                       aCCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCaga                                                                                                      >  1:3116818/1‑140 (MQ=255)
                                        ccTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCagag                                                                                                     <  1:199408/140‑1 (MQ=255)
                                          tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  1:1369115/1‑140 (MQ=255)
                                          tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  2:7132365/1‑140 (MQ=255)
                                          tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  1:1126349/1‑140 (MQ=255)
                                          tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  2:6336792/1‑140 (MQ=255)
                                          tctGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgc                                                                                                   >  1:2158946/1‑140 (MQ=255)
                                            tGACGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTGTTCAAAGCGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCCGGGgcgc                                                                                                 >  2:4193666/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGCACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  2:3211403/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:7655837/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:4589325/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:7208092/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCTTGCAGAGgcgc                                                                                                 >  1:492599/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  2:1239762/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:2757414/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  1:637902/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCAGCCGTTACAGGCTGAAGTTGGGAGAGCATAAGCCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGgcgc                                                                                                 >  2:5769804/1‑140 (MQ=255)
                                            tGAAGAACATTTTTCGAAATCAGTATTCAAAGCCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACAGCGCGGTTTAAGCGTCTCTGTCATGTTTTAATGAAGAGgcgc                                                                                                 >  1:1037148/1‑140 (MQ=255)
                                                   cATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCttttttt                                                                                          >  1:2505174/1‑140 (MQ=255)
                                                   cATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCttttttt                                                                                          >  2:3159860/1‑140 (MQ=255)
                                                      ttttCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAtt                                                                                       <  1:3783203/140‑1 (MQ=255)
                                                      ttttCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAtt                                                                                       <  2:2226949/140‑1 (MQ=255)
                                                      ttttCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGATGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGGTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAtt                                                                                       >  2:147043/1‑140 (MQ=255)
                                                        ttCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAAGCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAtttg                                                                                     >  1:1266656/1‑138 (MQ=255)
                                                        ttCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTgg                                                                                     >  2:6510617/1‑140 (MQ=255)
                                                          cGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGt                                                                                   <  2:6082254/140‑1 (MQ=255)
                                                           gAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTg                                                                                  <  2:5549714/140‑1 (MQ=255)
                                                               tCAGTAGTCCATGGCCGCCGCCGCCGGTACAGGCTGAAGTGGGGACCGCAGAAGCCTGCTTTTCAACCAGCGTCGAACCGCGCGGTTTAAGCGTCTCAGGCATGTTTTCATGGAGAGGCGCTTTTTTTATTGGGTGAaga                                                                              <  2:1001121/140‑1 (MQ=255)
                                                                    aTTCAAAGGCGGGCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGaaa                                                                         <  2:4866939/140‑1 (MQ=255)
                                                                           ggcggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATa                                                                  <  1:1550398/140‑1 (MQ=255)
                                                                             cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGTTGAAGAGGCAATCaacttga                                                                >  2:1957043/1‑135 (MQ=255)
                                                                             cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAATAGGAAATCAAATaga                                                                >  1:4107908/1‑140 (MQ=255)
                                                                              ggcCGCCGCCGTTACAGGCTGAATTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGAAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                               <  2:5410889/140‑1 (MQ=255)
                                                                              ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGTAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCGGTCATGTTTTCATGCAGAGGCGCTTATTTTATTGGGTGAAGAGGAAATCAAATagag                                                               <  2:1081026/140‑1 (MQ=255)
                                                                               gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTa                                                                                         <  2:1349569/113‑1 (MQ=255)
                                                                               gccgccgccGTTACAGGCGGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAACTCAAATAtaga                                                              >  2:7756579/1‑140 (MQ=255)
                                                                                ccgccgccgGTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGACATCAAATAGAGaa                                                             <  1:5429995/140‑1 (MQ=255)
                                                                                      ccgTTACAGTCTGAAGTTGGGAGAGCAGACGTCTGCTTTTCAAACAGAGTGGAAGCGCGCGGTTTAAGCGTGTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAATGGGTGAAGAGGAAATCAAATAGAGAAGTgtgg                                                       <  2:3605329/140‑1 (MQ=255)
                                                                                      ccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTgggg                                                       <  2:4959300/140‑1 (MQ=255)
                                                                                       cgTTACAGGCTGAAGTTGGGAGAGCAGAACTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGACGAAATCAAATAGAGAAGTggggg                                                      <  1:297271/140‑1 (MQ=255)
                                                                                            ctggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGc                                                 <  1:55823/138‑1 (MQ=255)
                                                                                                             agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                               >  1:6481387/1‑109 (MQ=255)
                                                                                                             agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATATAGAAGTGGGGGGAAGCAGGTGTTTTTTATAATg                                >  2:7435922/1‑140 (MQ=255)
                                                                                                               cAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAACCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCt                              <  2:7162137/140‑1 (MQ=255)
                                                                                                                aGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTc                             <  1:1215289/140‑1 (MQ=255)
                                                                                                                    ctCTGCTTTTCAAACAGAGTCGAACCGCGCGGTTTAAGAGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                         <  2:4579365/139‑1 (MQ=255)
                                                                                                                      cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  1:2887549/140‑1 (MQ=255)
                                                                                                                      cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  2:2757414/140‑1 (MQ=255)
                                                                                                                      cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  2:6731723/140‑1 (MQ=255)
                                                                                                                      cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  2:2390121/140‑1 (MQ=255)
                                                                                                                      cTGCTTTTCAAACAGAGTGGAACCGCGCGGGTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                       <  2:6417673/140‑1 (MQ=255)
                                                                                                                       tGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaag                      >  2:7292507/1‑140 (MQ=255)
                                                                                                                       tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTTAAGAGGAAATCAAAAATAGAATTGGGGGGAAGCATTTGTTTTTTATAATTCTCAaagaag                      >  2:465717/1‑140 (MQ=255)
                                                                                                                       tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCaa                           <  1:4829142/135‑1 (MQ=255)
                                                                                                                       tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                         >  2:5950839/1‑137 (MQ=255)
                                                                                                                       tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGTAAGAGGAAATCAAATAGAGAAATGGGGGGAAGCATGTGTTTTTTTGAATGCTCcaaaaca                      >  2:3058477/1‑136 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGGGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  1:6907071/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  1:926463/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  1:7760342/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:3733451/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  1:1851217/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:5138803/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:2661147/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTAGTGGGTGAAGAGGAAATCACATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  2:5287853/140‑1 (MQ=255)
                                                                                                                        gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCGGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                     <  1:3339533/140‑1 (MQ=255)
                                                                                                                              cAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAATAGTGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGATAAAAGAAGAAATTGa               >  2:3406624/1‑140 (MQ=255)
                                                                                                                                aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa             <  1:1709588/140‑1 (MQ=255)
                                                                                                                                aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa             <  1:3630643/140‑1 (MQ=255)
                                                                                                                                aaCAGAGTGGAACCGCGCGGTTTAAGCGTCTCGGTCATGTTTTCATGCAGAGGCGCTTTTTTTATGGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa             <  1:5042025/140‑1 (MQ=255)
                                                                                                                                aaCAGAGTGGAACAGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa             <  1:5740490/140‑1 (MQ=255)
                                                                                                                                       tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt      <  2:6169038/140‑1 (MQ=255)
                                                                                                                                       tGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt      <  2:2091186/140‑1 (MQ=255)
                                                                                                                                       tGGAACCGCGCGGTTTAAGCGTCTCGGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGtt      <  2:3319375/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGAGATCCATGAGCCGGGTTTTAGAAGTGCGCCCTTAAGACCTCTGAAGAACATTTTTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAT  >  NZ_CP034943/112392‑112670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: