Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,299,548 T→G 6.8% intergenic (‑246/+63) EO946_RS17065 ← / ← gap phosphoglycerate kinase/type I glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,299,5480TG 6.8% 1180.3 / 4.0 480intergenic (‑246/+63)EO946_RS17065/gapphosphoglycerate kinase/type I glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (161/245);  new base G (55/2);  total (233/247)
Fisher's exact test for biased strand distribution p-value = 5.51e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 497 total aligned reads displayed.

GGGCAGTTCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGTTATCGTACCAAG  >  NZ_CP034943/3299410‑3299687
                                                                                                                                          |                                                                                                                                           
gggCAGTTCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTGTATCACACAACCTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCccctt                                                                                                                                            >  1:2735657/1‑140 (MQ=255)
 ggCAGTTCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTGTGGCGATCACAGAACGTTTTGTGGAAGCCGGTCTTCATTGAAAAAGGGAAAGGGAGTGCGGCcccgtc                                                                                                                                           >  1:667636/1‑140 (MQ=255)
 ggCAGTTCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTGTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCcccttc                                                                                                                                           >  1:6205813/1‑140 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTGGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCGATCACCGAACGGTTTGTGAAACCCGGTCTTCAAGGAAAAAAGGCAAGGGAGTCcgtccccgtcccggg                                                                                                                                      >  1:2427339/1‑137 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTGTCTATCACAGAACGTTTTGTGAAAGCCGGTCTTAATGGAAAAATGGAAAGGGAGTCCGTCCCCGTCCCGTg                                                                                                                                      >  1:3550920/1‑140 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTg                                                                                                                                      >  2:4020405/1‑140 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTg                                                                                                                                      >  2:162405/1‑140 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCGATAACATAACGGTTTGTGAAAGCCGGTGTTGATGGAAAAATGGCAAGGGAGTCCGTCCCCGTCCCGTg                                                                                                                                      >  1:6988640/1‑140 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCGACCACAGAACGTTTTGTGAAAGCCGGTCTTCATGGAAAAATGGAAAGGGAGTACGTCCCCTTCCCTTg                                                                                                                                      >  1:4567975/1‑140 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTATCTATAACAGAACGTTTTCTTAAAGCCGGTGATGATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCGTg                                                                                                                                      >  1:7457849/1‑140 (MQ=255)
      ttCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTGTCTCTATCACAGAACGTTTTATGAAAGCCGGTGTTCATGGTAAAAGGGAAAGGGAGTCCGTCCCCGTCCCGTg                                                                                                                                      >  1:3757670/1‑140 (MQ=255)
        cGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTc                                                                                                                                    <  2:30964/140‑1 (MQ=255)
           cTGAAGCCAGGGCCGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGGCGTTTCACTATCACCGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGc                                                                                                                                 <  1:3620613/140‑1 (MQ=255)
           cTGAAGCCAGGGCAGCGGCCTTATTATTAATCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATAACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGc                                                                                                                                 >  2:482729/1‑140 (MQ=255)
           cTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTGTCTATCACAGAAAGTTTTCTGAAATCCGGTGTTGATGGAAAAATGGAAAAGGAGTCCCTCCCCGTGCCTTGTCCGc                                                                                                                                 >  1:1003032/1‑140 (MQ=255)
           cTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACATAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGGCCCCTTCCCATGTCCGc                                                                                                                                 >  2:1812992/1‑140 (MQ=255)
           cTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGc                                                                                                                                 <  1:6096398/140‑1 (MQ=255)
            tGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCt                                                                                                                                <  1:7680329/140‑1 (MQ=255)
             gAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCtt                                                                                                                               <  2:7629788/140‑1 (MQ=255)
              aaGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGCGTCCGTCCCCTTCCCTTGTCCGCttt                                                                                                                              <  1:5682575/140‑1 (MQ=255)
                gCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTGTATAACACAACGTTTTATGAAAGCCGGTCTTCATGGAAAAAGGGAGACGGAGTCCGTGCCCGTCCCGTGTCCGCtttga                                                                                                                            >  1:2893871/1‑138 (MQ=255)
                gCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTGTGAAAGCCGGTCTTCATTGAAAAAGGGAAAGGGAGTGCGTCCCCGTCCCGTGTCCGCTTTCa                                                                                                                            >  1:3267783/1‑140 (MQ=255)
                 ccAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCAt                                                                                                                           <  1:7542075/140‑1 (MQ=255)
                 ccAGGGCAGCCGCCATACGCGAACGCGCCGGCAAAGCAGCTAAAACCCCGGTCGTTTCTCTATCACAGAACGTTTTCAGAAAGCCGGTCTTCATGGAAAAAGGCAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCAt                                                                                                                           <  1:4253190/140‑1 (MQ=255)
                   aGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTGCCCTTCCCTTGTCCGCGTTCATta                                                                                                                         >  2:1906735/1‑140 (MQ=255)
                   aGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTACGTCCCCTTCCCTTGTCCGCTTTCATta                                                                                                                         >  2:810905/1‑140 (MQ=255)
                     ggCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTGTGTATCACAGAAAGTTTTATGTAAGCCGGTGTTGATTGTAAAATGGGAAGGGCGTGCGTCCCCGTCCCGTGTGCGCTTTGATtatt                                                                                                                       >  1:4995376/1‑139 (MQ=255)
                      gCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAg                                                                                                                      <  2:3184307/140‑1 (MQ=255)
                      gCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAg                                                                                                                      <  2:2661284/140‑1 (MQ=255)
                          cGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTGTGTATGACACTACGGTTTGTGAAAGCCCGTGTTCATGGAAAAGGGAAAGGGAGTCCGTCCCCGTCCCGTGTCCGCTTTCTATATATCGCtt                                                                                                                 >  1:584376/1‑140 (MQ=255)
                          cGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATAAAACAACGTTTTATGAAAGCCCGTCTTCAATGAAAAAAGGAAAGGGAGTCCGTCCCCGTCCCGTGTGCGCTTTAATTATATCGCt                                                                                                                  >  1:3054585/1‑140 (MQ=255)
                          cGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTAACACAGAACGTTTTCTGAAAGCCGGTCTTCATTGAAAAAGGGAAAGGGAGTCCGTCCCCGTCCCTTGTCCGCTGTCATTATAGCGCt                                                                                                                  >  2:7498377/1‑140 (MQ=255)
                             ccTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTGTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCttat                                                                                                               >  2:3568290/1‑138 (MQ=255)
                                    cGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGATTTCATTATAGCGCTTCTATTCaaa                                                                                                        <  1:5426673/140‑1 (MQ=255)
                                    cGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAAAGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCaaa                                                                                                        <  1:2233635/140‑1 (MQ=255)
                                       aaGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTGATGGAAAAAGGGAAAGGGAGTACGTCCCCATCCCGTGTGCGCTTTCATTATAGCGCTTCTATTCAAAAGa                                                                                                     >  2:2401989/1‑140 (MQ=255)
                                       aaGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTATTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCGTCCCTTGTCCGGTTTGATTATATCGCTTCTATTCAAAAGa                                                                                                     >  2:5428399/1‑140 (MQ=255)
                                       aaGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATAACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGa                                                                                                     >  2:7343813/1‑140 (MQ=255)
                                       aaGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATAAAAGAACGTTTTGTTAAATCCGGTCTTCATTGAAAAAGGGAAAGGGAGTCCGTCCCCGTCCCGTGTCCGCTTTCATTATATCGCTTATAATAaaaaaa                                                                                                     >  1:4110738/1‑138 (MQ=255)
                                       aaGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTGTCTCTATCACACAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCGTTCAGTATATCGCTTCTATTCACAAGa                                                                                                     >  2:7187170/1‑140 (MQ=255)
                                       aaGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTGAGTCTAGAACAGTACGTTTTCTGTAACCCGGTCTTCATGGGAAAAGGGAAAGGGCGTGCGGCCCCTGCCCTTGGCGGCTTTGATTATAGCGCGTGTATTCAAAAGa                                                                                                     >  2:2254259/1‑140 (MQ=255)
                                         gcgcCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAAc                                                                                                   <  1:1097462/140‑1 (MQ=255)
                                            ccGGCAAAGCTGCTAAAAACGCGGTCGTTTGTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATTGAAAAAGGGAAAGGGCGTCCCTCCCCTTCCCTTGTCCGCTTTCATTAAAGCGCTTCTATTAAAAATAACCaa                                                                                                >  2:6628854/1‑140 (MQ=255)
                                            ccGGCAAAGCTGCTAAAAACGCGGTCGTTTGTCTATCACAGAAAGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCGTCCCTTGTCCGCTTTCATTATAGCGCTTCGATTCAAAAGAACCaa                                                                                                >  2:4426982/1‑140 (MQ=255)
                                             cGGCAAAGCTGCTAAAAACGCGGTCGTTTGTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTACGTCCCCTTCCCTTGTCCGCTTTAATTATAGCGCTTCTATTAAAAAGAACCaaa                                                                                               >  2:4360913/1‑139 (MQ=255)
                                             cGGCAAAGCTGCTAAAAACGCGGTCGTTTGGCTATCAAAGAACGTTTTCTGAAACCCGGTCGTGCTGGAAAAATGGAAAGGGAGTCCGTCCCCTGCCCTTTTGCGCGTTGCTTATAGCGCGTCTATTAAAAAgctgtctc                                                                                               >  2:57132/1‑133 (MQ=255)
                                             cGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATACCATAACGTTTTCTGAAAGCCGGGCTTCATTGAAAAAGGGAAAGGGCGTCCGTACCCATACCGTGTCCGCTTTAAATATATCGCTTCTATTCAAAAGAACCAAg                                                                                               >  2:5123729/1‑140 (MQ=255)
                                             cGGCAAAGCTGCTAAAAACGCGGTCGTGTGTCGATACCACAACCTTTTGTGTAAGCCGGGCGGCATGGTAAAAAGGACACGGAGTCCGGCCCCCTCCCGTGTGCGCGGTCATTATAGCGCTTCTATTAAAAAGAACCAAg                                                                                               >  1:2102020/1‑140 (MQ=255)
                                              ggCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGt                                                                                              <  2:3783284/140‑1 (MQ=255)
                                               gCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTc                                                                                             <  1:4468092/140‑1 (MQ=255)
                                                   aGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTATTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGt                                                                                         <  2:4917212/140‑1 (MQ=255)
                                                    gctgctAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGGTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTc                                                                                        <  2:6294618/140‑1 (MQ=255)
                                                    gctgctAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTc                                                                                        <  2:2525990/140‑1 (MQ=255)
                                                          aaaaaCGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCtat                                                                                  <  1:6570167/140‑1 (MQ=255)
                                                                             tATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCttttttt                                                               <  1:2333792/140‑1 (MQ=255)
                                                                             tATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCttttttt                                                               <  2:7399857/140‑1 (MQ=255)
                                                                              aTCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTg                                                              <  2:5031070/140‑1 (MQ=255)
                                                                                cacaGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCg                                                            <  2:4638484/140‑1 (MQ=255)
                                                                                cacaGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCg                                                            >  1:5531758/1‑140 (MQ=255)
                                                                                cacaGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCg                                                            <  1:3155690/140‑1 (MQ=255)
                                                                                 acaGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGa                                                           <  1:1816469/140‑1 (MQ=255)
                                                                                    gAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGt                                                        <  1:917546/140‑1 (MQ=255)
                                                                                    gAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGt                                                        <  2:3306879/140‑1 (MQ=255)
                                                                                     aaCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATCAATTAAAGACCTTTTTTTGCGATGTa                                                       >  2:2832788/1‑140 (MQ=255)
                                                                                     aaCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCttttttt                                                               >  1:6329303/1‑132 (MQ=255)
                                                                                     aaCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTa                                                       >  1:1738789/1‑140 (MQ=255)
                                                                                     aaCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTa                                                       <  1:4634236/140‑1 (MQ=255)
                                                                                     aaCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTa                                                       <  2:533857/140‑1 (MQ=255)
                                                                                     aaCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTa                                                       >  1:806777/1‑140 (MQ=255)
                                                                                     aaCGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCAATATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATCAATTAAAGACCTTTTGTTTCGATGTa                                                       >  2:2141321/1‑140 (MQ=255)
                                                                                       cGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAg                                                     >  1:4838773/1‑140 (MQ=255)
                                                                                       cGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAg                                                     >  1:1505716/1‑140 (MQ=255)
                                                                                           ttCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCTCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGc                                                 <  2:1093623/140‑1 (MQ=255)
                                                                                           ttCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCACAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTGTGCGATGTAAGCTGc                                                 <  2:6069647/140‑1 (MQ=255)
                                                                                            tCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCa                                                <  1:5591210/140‑1 (MQ=255)
                                                                                            tCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGGCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCa                                                <  2:4471648/140‑1 (MQ=255)
                                                                                                      ggTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCCGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTcaacaa                                      <  2:590056/140‑1 (MQ=255)
                                                                                                      ggTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTcaacaa                                      <  1:3320314/140‑1 (MQ=255)
                                                                                                      ggTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTcaacaa                                      <  1:1960882/140‑1 (MQ=255)
                                                                                                      ggTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTcaacaa                                      <  1:7343813/140‑1 (MQ=255)
                                                                                                      ggTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTcaacaa                                      <  2:2950010/140‑1 (MQ=255)
                                                                                                       gTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCaacaac                                     <  2:5217567/140‑1 (MQ=255)
                                                                                                       gTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCaacaac                                     <  2:3002355/140‑1 (MQ=255)
                                                                                                       gTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCaacaac                                     <  2:4170813/140‑1 (MQ=255)
                                                                                                            cATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCCAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGt                                <  2:3859979/140‑1 (MQ=255)
                                                                                                            cATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGt                                >  1:1611366/1‑140 (MQ=255)
                                                                                                            cATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGt                                <  1:1255042/140‑1 (MQ=255)
                                                                                                                  aaaaGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGt                          <  2:1746788/140‑1 (MQ=255)
                                                                                                                    aagggaaagggaGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAg                        <  1:3903259/140‑1 (MQ=255)
                                                                                                                     agggaaagggaGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGc                       <  2:1224368/140‑1 (MQ=255)
                                                                                                                     agggaaagggaGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGc                       <  2:701189/140‑1 (MQ=255)
                                                                                                                     agggaaagggaGTCAGTCCCCTTCCCTTGTCCGCTTTAATTATAGCGATTCTATTCAAAAGAACAAATTCAGGTCAGCTATAAATTAAAGACCTTTTTTTGCGTTGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGc                       <  2:1468677/140‑1 (MQ=255)
                                                                                                                           agggaGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCttt                 <  1:2534970/140‑1 (MQ=255)
                                                                                                                           agggaGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCttt                 >  1:5077532/1‑140 (MQ=255)
                                                                                                                                gtccgtccCCTTCCCGTGTCCGCTTTCATTATAGCGCTTCTATGCAAAAGAACCAAGTCAGGTCCGCGCGAAATTAAAGACCTTTTTGTGCGATGTACGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGttc            >  1:5198422/1‑139 (MQ=255)
                                                                                                                                 tccgtccCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGTTAt           >  2:3662630/1‑140 (MQ=255)
                                                                                                                                 tccgtccCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGTTAt           >  1:6793337/1‑140 (MQ=255)
                                                                                                                                         cttcccttGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGTTATCGTACCaa   >  1:5947059/1‑140 (MQ=255)
                                                                                                                                          ttcccttGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGTTATCGTACCAAg  >  1:7168616/1‑140 (MQ=255)
                                                                                                                                          ttcccttGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACATTTTTTTGCGGTGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGTTATCGTACCAAg  >  1:1353633/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GGGCAGTTCGTCTGAAGCCAGGGCAGCGGCCTTAGGCGTAAGCGCCGGCAAAGCTGCTAAAAACGCGGTCGTTTCTCTATCACAGAACGTTTTCTGAAAGCCGGTCTTCATGGAAAAAGGGAAAGGGAGTCCGTCCCCTTCCCTTGTCCGCTTTCATTATAGCGCTTCTATTCAAAAGAACCAAGTCAGGTCCGCTATAAATTAAAGACCTTTTTTTGCGATGTAAGCTGCAAGGTCAACAACGCGGTTAGAGTAGCCGCTTTCGTTATCGTACCAAG  >  NZ_CP034943/3299410‑3299687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: