BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,362,393	G→C	9.8%	L99L (CTG→CTC)	EO946_RS17380 →	MazG‑like family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,362,393	0	G	C	9.8%	1292.1 / 18.2	460	L99L (CTG→CTC)	EO946_RS17380	MazG‑like family protein
Reads supporting (aligned to +/- strand): ref base G (264/139); new base C (2/54); total (266/194)
Fisher's exact test for biased strand distribution p-value = 2.26e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 465 total aligned reads displayed.

ATGAGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCT  >  NZ_CP034943/3362254‑3362532

atgaGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTg                                                                                                                                             >  2:1934434/1‑140 (MQ=255)

atgaGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTg                                                                                                                                             >  1:6052124/1‑140 (MQ=255)

 tgaGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGa                                                                                                                                            >  2:5530550/1‑140 (MQ=255)

  gagaAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGTAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGAc                                                                                                                                           <  2:1335769/140‑1 (MQ=255)

  gagaAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGAc                                                                                                                                           <  1:5956165/140‑1 (MQ=255)

  gagaAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGAc                                                                                                                                           <  2:1735784/140‑1 (MQ=255)

     aaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                        <  1:7190759/140‑1 (MQ=255)

     aaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                        >  1:1354578/1‑140 (MQ=255)

     aaagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                        >  2:2947385/1‑140 (MQ=255)

     aaagaaTCGACACGAGCTGAGAAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                        <  2:1373139/140‑1 (MQ=255)

      aagaaTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaaa                                                                                                                                       <  2:362085/140‑1 (MQ=255)

          aTCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACAAAACggt                                                                                                                                   >  1:7111535/1‑138 (MQ=255)

                  gAGCTGAGCAAAACCAAGAATTGATTGAAGAAATGGGGCCTGTCATCGGAAATCTCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAgcat                                                                                                                           <  2:7087859/140‑1 (MQ=255)

                        aGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATaa                                                                                                                     >  2:623185/1‑140 (MQ=255)

                        aGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATaa                                                                                                                     >  1:2948354/1‑140 (MQ=255)

                           aaaaaaaaTAATTGATTGAAGATTTGGGGTATGTATTGGGAAATATCGCAATTTTTGCTGATATTTACGGAGTCTGTTTATAAGAGGTTATGAAAGCCCACCAAGAAAAACTGACAAAACTTTTTGAGAATGAATaaaaa                                                                                                                  <  2:2566067/140‑1 (MQ=255)

                              aaCAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCaa                                                                                                                                        <  1:1094416/115‑1 (MQ=255)

                                         attgaAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATgg                                                                                                    >  2:18005/1‑140 (MQ=255)

                                         attgaAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATgg                                                                                                    >  1:6119057/1‑140 (MQ=255)

                                         attgaAGAAAGGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATgg                                                                                                    >  1:320378/1‑140 (MQ=255)

                                                   tGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCtttttt                                                                                          >  2:4001441/1‑140 (MQ=255)

                                                   tGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCtttttt                                                                                          >  1:2020817/1‑140 (MQ=255)

                                                   tGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGAGGCCGTAATGGCCGCtttttt                                                                                          >  2:1356878/1‑140 (MQ=255)

                                                       ggATGTCATCGTAAATATCGCCTTTGTTGCTGATCTGTCCGGAGTCTCTTTAGAAGAGGTGATGAAACCCCACAAAGAAAACCGCACCAAACGTTTTCAGCAGGCATAAAAACGCGGCCGTAATGGCCGCTTTTTTTAAc                                                                                      <  1:231190/140‑1 (MQ=255)

                                                       ggATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAATACGTAATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTTAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTaaa                                                                                      >  2:1072708/1‑139 (MQ=255)

                                                       ggATGTAATCGGAAATATCGCCATTCTTGCTGATATGTACGCAGTCTCTTTAGAAGACTTGATGAAAGCCCACCACGAAAAACTGCCCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAc                                                                                      <  2:4719519/140‑1 (MQ=255)

                                                          tGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCtg                                                                                   >  1:2690633/1‑140 (MQ=255)

                                                          tGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCtg                                                                                   >  1:5163522/1‑140 (MQ=255)

                                                                      tatCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTcatcat                                                                       >  2:893451/1‑140 (MQ=255)

                                                                      tatCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGACGCTTTTTTTAACCTGTGATTTcatcat                                                                       >  2:7650234/1‑140 (MQ=255)

                                                                       atCGCCATTCTTGCTGTTCTGTCCGTAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATg                                                                      <  2:6900481/140‑1 (MQ=255)

                                                                            cATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGAt                                                                 <  2:6119057/140‑1 (MQ=255)

                                                                              ttCTTGCTGATATGTACGGAGTCTCTTTACACGACGTGATCAACGCCCACCAAGAAAAACTCACCCACCGTTTTGAGCATCCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCa                                                               <  1:3462335/140‑1 (MQ=255)

                                                                               tCTTGCTTATATGTACGCAGTCTATTTAGAAGACGTGATTAACGCCCACCAAGAAAAACTGACCCAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCaa                                                              <  2:813807/140‑1 (MQ=255)

                                                                                   gCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCGGTGATTTCATCATGGTGATCACAGaa                                                          >  1:698267/1‑140 (MQ=255)

                                                                                         atGTACGGAGTCTCTTTAGAAGACGTGATTAACGCCCACCAAGAAAAACTGCCCAACCGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                    <  1:1799151/140‑1 (MQ=255)

                                                                                         atGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAAtgctg                                                    <  2:1616712/140‑1 (MQ=255)

                                                                                           gTACGGAGTCTCTTTACAAGACGTGATGAAAGCCCACCAAGAAAACCTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAACTgctgct                                                  <  2:715386/140‑1 (MQ=255)

                                                                                              cGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTc                                               >  2:3542958/1‑140 (MQ=255)

                                                                                              cGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTc                                               >  1:4416367/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGTCCGCTTTTTTTAAACAGTGATTTCATAATGGTTATGAAATAAATtctgctataata                                            >  1:4866372/1‑134 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAGCTGTGATTTCATCCTGTTGATCAAAGAAATGCTGCTTTACTa                                            >  1:1025343/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAATAAATGCTGCTTTCCTa                                            >  1:3999692/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAATAAATGCTGCTTTACTa                                            >  1:6815638/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATCATGGTGATCAAAGAAATGCTTCTTTCCTa                                            >  2:5188046/1‑140 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTGTAACCTGTGATTTCATCAGGGTGATCCAATCACTTCTGCGTTCCtg                                            >  1:573224/1‑139 (MQ=255)

                                                                                                 aGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGAGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCAGAATGGTGATCCAAGAAATGCTGGTTTCGTa                                            >  1:6629423/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGTTTTCCTCAGGGTGATCAAAGAAATGCTGCTTTCCTAtt                                          >  1:3925342/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATTCTGCTTTCCTAtt                                          >  1:1969822/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAtt                                          >  1:7630821/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCGAtt                                          >  1:6439188/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATAATGGTGATCAAAGAAATGCTGCTTTCCTAtt                                          >  2:6736977/1‑140 (MQ=255)

                                                                                                   tctcTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTAATCATGGTGATCAAAGAAATGCTGCTTTCCTAtt                                          >  1:6369161/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTTTGATTTCATCATGGTGATCAAATAAATGCTGCTGTCCTATGTtg                                       >  2:6327037/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATTCTGCTTTCCTAttttt                                       >  1:4587222/1‑139 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTAtttgg                                       >  1:1550279/1‑138 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                       >  1:5850222/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                       >  1:3564633/1‑140 (MQ=255)

                                                                                                      ctTTAGAAGACGTGATGAAAGCCCACCAACAAAAACTGACCAAACGTTTTGACCATGCAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTtg                                       <  2:4974594/140‑1 (MQ=255)

                                                                                                           taacaCGTGATGAAAGCCCACAAACAAAACCTCACCAAACGTTTTGAGAATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  1:6252792/136‑1 (MQ=255)

                                                                                                           gaagaGGTGATGAAAGCCCACCAACAAAAACTGACCAAAGGTTTTGAGCATCCATAAAAAAGCGGCCGTAAGGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  1:2200652/140‑1 (MQ=255)

                                                                                                           gaagaCGTTATGAAAGCCCACCAATAAAAACTGACCAAACGTTTTGAGCATGCATAAAAACGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  1:251860/140‑1 (MQ=255)

                                                                                                           gaagaCGTTATGAAAGCCAACCAAGAAAAACTGACCAAACGTTTTGAGCATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  2:2361343/140‑1 (MQ=255)

                                                                                                           gaagaCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAACAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  2:7392217/140‑1 (MQ=255)

                                                                                                           gaagaCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  >  1:3316732/1‑140 (MQ=255)

                                                                                                           gaagaCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGAATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  2:2265927/140‑1 (MQ=255)

                                                                                                           gaacaCGTGCTTAAAGCCCACAACGAACCCCTGAGCAAGCGTTTTTAGCCTGCAGAAAAAAGCTGCCGGAATGGCCGCTTTTTATAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGaaa                                  <  1:4278804/140‑1 (MQ=255)

                                                                                                             agaCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAATAAATGCTGCTTTCCTATTTTGTGAAATa                                >  1:7452304/1‑140 (MQ=255)

                                                                                                               aCGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTGGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAGCTGTGATTTCATCATGGGGATCAAAGTAATGCTGCTTTCCGATTTTGTGAAATAgg                              >  1:5636455/1‑140 (MQ=255)

                                                                                                                ctttattaaaGCCCACCAACAAAAACTCACCAAACGTTTTTAGCATGAATAAAAAAGCGGCCGTTATGGCAGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:4525630/133‑1 (MQ=255)

                                                                                                                cttgtggaAAGCCCACCATGAAAACCTCCCCACTCGTTTTGAGCCTGCATAAAACAGCGGCCGTAATGGCCGCTATTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:3433836/134‑1 (MQ=255)

                                                                                                                cGTGATTAAAGCCCACCCAGAAAACCGGCCCACAGGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  1:3752750/140‑1 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAATAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:5016572/140‑1 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAAGAAACACTGACCAAACTTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:5436703/140‑1 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGGAATAGGc                             >  1:1177677/1‑140 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:2690633/140‑1 (MQ=255)

                                                                                                                cGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGACCTGGCATAAAAAAGCGGCCGGACTGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  1:5434678/140‑1 (MQ=255)

                                                                                                                cGTGATCAAAGCCCACCAAGAACAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGc                             <  2:4857501/140‑1 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGCCCAAACGTGTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGGTTGCTTCATGGTGATCAAATAAATGCTGCTTTCCTATTTTGTGTAATAGGcc                            >  2:3176639/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAATAGCGGCCGTAATGGCCTCTTTTTTTAACCGGTGATTTACAAATGGTGATAAAAGAAAAGCTTGTTTCATAATTTGTGAAATAGGcc                            >  1:4974184/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATATGcc                            >  2:109961/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:4670438/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:302042/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  1:4892822/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:4088996/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTACTATTTTGTGAAATAGGcc                            >  1:4202488/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATAATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:6440784/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAAACTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            >  2:5245744/1‑140 (MQ=255)

                                                                                                                 gTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTAAAACTGTGATTTCATCAAGGTGATCAAAGAAATGCTGCTTTACTATTTTGTGAAATAGGca                            >  2:7331137/1‑139 (MQ=255)

                                                                                                                 gTGATCAAAGCCCACCAAGAAAAACTCACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGcc                            <  1:949663/140‑1 (MQ=255)

                                                                                                                      gaAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGAAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTGTCCTAGTTTGTGAAATAGGCCTGttt                       >  2:5695435/1‑140 (MQ=255)

                                                                                                                              accaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagc               >  1:6210832/1‑140 (MQ=255)

                                                                                                                              accaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgaagc               >  1:4994706/1‑140 (MQ=255)

                                                                                                                               ccaAGAAAACCGCACCAAACGTTTTGACCATGCACAAAAAAGCGGCCGTACTGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagca              <  1:5576867/140‑1 (MQ=255)

                                                                                                                               ccaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagca              <  1:893451/140‑1 (MQ=255)

                                                                                                                               ccaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagca              >  2:7473688/1‑140 (MQ=255)

                                                                                                                               ccaAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagca              >  2:4289491/1‑140 (MQ=255)

                                                                                                                               caaaGAAAAACTGACCAAACTTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagca              <  2:6586323/138‑1 (MQ=255)

                                                                                                                               caaaGAAAAACTGAAAAACGGTTTTCCGCATGAAAAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATgcagca              <  1:4755977/138‑1 (MQ=255)

                                                                                                                                           gACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGATTTTTTTAACCTGTGAATTCATCATGGTGTTCAATGAAATGGTGCTTTCCTATTTTGTGAAATAGGACTGTTTTATGCAGCAGCTCCTTCGGCt  >  2:4138362/1‑140 (MQ=255)

ATGAGAAAGAATCGACACGAGCTGAGCAAAAACAAGAATTGATTGAAGAAATGGGGGATGTCATCGGAAATATCGCCATTCTTGCTGATATGTACGGAGTCTCTTTAGAAGACGTGATGAAAGCCCACCAAGAAAAACTGACCAAACGTTTTGAGCATGCATAAAAAAGCGGCCGTAATGGCCGCTTTTTTTAACCTGTGATTTCATCATGGTGATCAAAGAAATGCTGCTTTCCTATTTTGTGAAATAGGCCTGTTTTATGCAGCAGCTCCTTCGGCT  >  NZ_CP034943/3362254‑3362532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`