BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,394,434	T→G	5.9%	S156R (AGC→CGC)	ppaX ←	pyrophosphatase PpaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,394,434	0	T	G	5.9%	1307.4 / 9.7	503	S156R (AGC→CGC)	ppaX	pyrophosphatase PpaX
Reads supporting (aligned to +/- strand): ref base T (206/241); new base G (52/2); total (260/243)
Fisher's exact test for biased strand distribution p-value = 5.00e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 504 total aligned reads displayed.

AATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCTTAATTT  >  NZ_CP034943/3394299‑3394572

  tCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAg                                                                                                                                      <  2:1094873/140‑1 (MQ=255)

   cAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCCTAATGGCTTCCGCCGGCTCGCTTCCTAGc                                                                                                                                     <  1:2563692/140‑1 (MQ=255)

    aGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCGTTTTTGCCGGCCCGCACCGCGTGGTAATTATCAGCGGCCCTAATTGGTTCCGCCGGCGCGCGTGCTAGcg                                                                                                                                    >  1:7613469/1‑139 (MQ=255)

     ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTGCCTGCCCCCACAACGGGGGCAATATCTACGACCAAAATGGGTTCCGCCGGCGCGCTTccgaacgg                                                                                                                                   >  1:1374042/1‑134 (MQ=255)

     ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTACGGCGTTTTTCCCAGCCAGCACCTCGTGGTAATTATAACCGACCCAAATGGGATCCCCCGGCTCGCTTCCTAGCTg                                                                                                                                   >  2:6027533/1‑140 (MQ=255)

     ggCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTCCGGCGTTTTTGCCTGCCACCCCCTCGTGGTAATTATATGCGACCATAAATGCTTCCGCCCGCGCGCTTGCTAGCTg                                                                                                                                   >  1:4001828/1‑140 (MQ=255)

     ggCTCATGCGTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCCTTTTTCCCTGCCAGCACAGCGGGGTAAATATCACCGACCATAATGGCATCCGCCGGCACCCTTccacgcgg                                                                                                                                   >  2:3778720/1‑134 (MQ=255)

      gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGGGTTTTTTCCTGCCAGCACAACGTGGTAATTATGTCCCACCAAAATGGGTTCCGCCGGCGCGCGTGCGAGCTGt                                                                                                                                  >  1:1907790/1‑140 (MQ=255)

      gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTAGCATCCAGCACATCGTGGTAATTATGTAGGACCAAAAATGCTTACGCCCGCTCGCTTCGAAGCTGt                                                                                                                                  >  1:3831255/1‑140 (MQ=255)

      gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGGTTTTTTTGCGGCGTTTTTACCTGCCAGCACAGCGGGGGAATTATCTCCGACCATAATGGCTTCCGCCGGCACGCTTCCTAGCTGt                                                                                                                                  >  2:1710772/1‑140 (MQ=255)

      gCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGGTTTTTTTGCGGCGGTTTTGCCTGCCCGCACAGAGGGGGACTTATCTCCGGCCAGACTGGCATCCGCCGCCTCGCGTCCTCGCGGt                                                                                                                                  >  1:5570611/1‑140 (MQ=255)

          aTGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGGTTTTGCCGGCCAGGACAACGTGGTAATTATCTCCGACCCAAAATGCTTCCGCCGGCGCGCGTCCGAGCTGgttaa                                                                                                                              >  1:1782235/1‑138 (MQ=255)

           tGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTCCCTGCCAGCACATCGTGGTAATTATCTACGACCATAAAGGCTTACGCCGGCTCGCTTACTAGCTGTTTCAg                                                                                                                             >  2:5506640/1‑140 (MQ=255)

               ttGCGAGTGTTTCCGGCGCTTTAATCGTCCTTGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTGCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCt                                                                                                                         <  2:3923353/140‑1 (MQ=255)

                tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTTTTCCGGCGGTTTTCCCTGCCAGCACAACGTGGTATGTTTATCCGACCATAATGTCTTGCGCCGGGTCGCTGCCTAGCTGTTTCAGCGCtt                                                                                                                        >  1:5752588/1‑139 (MQ=255)

                tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTATTTACGGCGTGTTTCCCAGCCACCAGAACGGGGTAAATATATACGACCAAAAATGCTTCCGCCGCCGCGCATACTAACAGTTTCAACGCTa                                                                                                                        >  2:1843521/1‑140 (MQ=255)

                tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTTCGGCGGTTTTCCCCGCCCGCCCCACGGGGTGATTATATACGACCAAAAATTCGTGCGCCGGCGCGCTTCCTAGCTGTTTGAgcgcga                                                                                                                        >  1:3478705/1‑138 (MQ=255)

                tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTGCCGGCCAGCACAACGTGGTAAATATATCACACCAAAAATGCTTACGCCCCCTCGCGTCCGATCGGTTTTAgcgcga                                                                                                                        >  1:1013652/1‑138 (MQ=255)

                tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTACCAGCCCGCACAACGTGGTAAATATATCCCACCAAAAATGCTTACGCCCGCTCGGTTGCCATCTGTTTAAGCCCTa                                                                                                                        >  1:4933883/1‑140 (MQ=255)

                tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGGTTTTGCCTGCCAGCACAACCTGGTAAATAAATCCCCCCCAAAATTCTTCCCCCGGCGCGCGTCCAAGCGGGTTAAgcgcaa                                                                                                                        >  1:6562183/1‑138 (MQ=255)

                tGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTGTTGCGGCGTTTTTACCTGCCCGCACCTCGTGGTAATTATATCCGACCATAAAGGCATCCGCCCGCGCGGTTCCAAGCTGTTTCACCGCTa                                                                                                                        >  1:5303194/1‑140 (MQ=255)

                 gCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                                       <  1:3261377/140‑1 (MQ=255)

                 gCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTaa                                                                                                                       <  2:5844305/140‑1 (MQ=255)

                      tgtTTCCGGCCCTTTACTCGTCCATGCGACTCAGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATTGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                                  <  1:5972278/140‑1 (MQ=255)

                      tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTACGGCGTTTTTCCCTGCCAGCACCTCCTGGTAAATATATGCCACCATAAAGGCTTCCCCCGGCTCGCGTGCGAGCTGTGTCAGCGCGAAAAGcc                                                                                                                  >  2:5224469/1‑139 (MQ=255)

                      tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTGTGGTCCGGCGTTTTTCCCTGCCAGCACATCGGGGGAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGca                                                                                                                  <  1:1551925/140‑1 (MQ=255)

                      tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTGCGGCGTTTTTCCCTGCCCGCCCATCGTGGTAATTATATCCGACCATAATGGGTTACCCCGGCTCGGGGCCGATGGGTTTAAGCGGTAAAAGca                                                                                                                  >  1:5631938/1‑140 (MQ=255)

                      tgtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTGTTTTTTCAGGCGTTTTTAACAGCCAGCACATCCTGGTAATTATATCACACCAAAATTGGTTCCCCCCGCCCCCTTCAGAGATTTTTAATAGCTaaaatac                                                                                                                  >  2:5126068/1‑137 (MQ=255)

                      tgtTTCCGGCCCTTTAATCGGCCAGGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTCTCTCCGACCATAAGGGCTTCCGCCGGCTCGCTTCCTATCTGTTTCAGCGCTAAAAGca                                                                                                                  <  2:676732/140‑1 (MQ=255)

                       gtTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGcac                                                                                                                 <  1:721409/140‑1 (MQ=255)

                            cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                                            <  1:3670259/140‑1 (MQ=255)

                            cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                                            <  1:6892567/140‑1 (MQ=255)

                            cGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCt                                                                                                            <  1:747189/140‑1 (MQ=255)

                                  tttAATCGTCCATGCGACTCCGGCCGTTTTTTTTGCGGCGTTTTTTCCTGCCACCACAAGGTGGTTATTTTGTCGGCGCAAAATGGGTTGCGCCGGCTCGCGTGCGAGCTGTTTGAGAGCGAAAATCACACGcgggggct                                                                                                      >  1:4424696/1‑133 (MQ=255)

                                  tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                                                                                                      <  1:2197623/140‑1 (MQ=255)

                                  tttAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGAt                                                                                                      <  1:2259375/140‑1 (MQ=255)

                                  tttAATCGTCCATGCGACTCCGGCCGTGTTTTTTCCGGCGGTTTTACCAGCCCGCACATCGTGGGAATTATATCCGACCATAAAGGGTTCCGCCGGCTCGGTTCCTAGCTGTTTCAGCGCGAAAAACACCGGCTCGGGAt                                                                                                      >  2:2747234/1‑140 (MQ=255)

                                      aTCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAgg                                                                                                  <  2:5631938/140‑1 (MQ=255)

                                          tCCATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTACCAGCCCGCACATCGTGGTAATTATATACGACCATAAATGCTTGCGCCGGCTCGCTTCCTAGCGGTTTCAGCGCTAAAAACACAGGCGCGGGATCAGGCgtt                                                                                              >  2:2682269/1‑140 (MQ=255)

                                            cATGCGACTCCGGCCGTTTTTGTTGCGGCGTTTTTCCCGGCCCGCACCTCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                                                                                            >  2:739348/1‑140 (MQ=255)

                                            cATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                                                                                            <  2:6163227/140‑1 (MQ=255)

                                            cATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCGTCCCCCGGGTCGCGTACTAGCGGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGc                                                                                            >  2:4763248/1‑140 (MQ=255)

                                            cATGCGACTCCGGCCGTGTTTGTTGCGGCGGTTTTACCGGCCAGCACCTCGTGGTAATTATCGCCGACCATAATGGCTTCCGCCGGCTCGCTTCCAAGCTGGTTGAGCGCTAAAAGCACCCGCTCGGGGTCAGGCGTTGc                                                                                            >  2:2979245/1‑140 (MQ=255)

                                              tGCGACTCCGGCCGTTTTTGTTCCGGCGGTTTTCCCTGCCAGCACCGCGTGGTAATTATCTCCGACCATAATGGCGTCCCCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACACGCTCGGGCTCCGGCGTTGCCa                                                                                          >  2:6519214/1‑140 (MQ=255)

                                                    tCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTca                                                                                    <  1:4596013/140‑1 (MQ=255)

                                                     ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                                   <  1:6991983/140‑1 (MQ=255)

                                                     ccGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcac                                                                                   <  1:4248813/140‑1 (MQ=255)

                                                      cGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcaca                                                                                  <  2:4963967/140‑1 (MQ=255)

                                                      cGGCCGTTTTTGTTCCGGCGTTTTTCACTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTcaca                                                                                  <  1:1627592/140‑1 (MQ=255)

                                                           gTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACatcatc                                                                             <  2:4642237/140‑1 (MQ=255)

                                                            tttttGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                            <  1:73619/140‑1 (MQ=255)

                                                            tttttGTTCCGGCGTTTGTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACAtcatca                                                                            <  1:6746757/140‑1 (MQ=255)

                                                                              tCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCa                                                          <  1:2901852/140‑1 (MQ=255)

                                                                                  tGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCt                                                                                                                         >  2:1543651/1‑73 (MQ=255)

                                                                                        cacaTCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGc                                                <  1:4867636/140‑1 (MQ=255)

                                                                                             cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                                           >  2:2214756/1‑140 (MQ=255)

                                                                                             cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGAtt                                           >  2:3042266/1‑140 (MQ=255)

                                                                                             cGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCACCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCCCATCATCAAGGGTAACGACCGTTTCAATGAATTCGCCGAtt                                           >  2:3985208/1‑140 (MQ=255)

                                                                                              gTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCTTTTCAAAGAATTCGCCGATTc                                          >  2:4271425/1‑140 (MQ=255)

                                                                                              gTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAATAATTCGCCGATTc                                          >  2:6895059/1‑140 (MQ=255)

                                                                                                  tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                      <  2:4483697/140‑1 (MQ=255)

                                                                                                  tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                      <  2:7207376/140‑1 (MQ=255)

                                                                                                  tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                      <  1:115051/140‑1 (MQ=255)

                                                                                                  tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                      <  2:1974694/140‑1 (MQ=255)

                                                                                                  tAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCGAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                      <  2:2483547/140‑1 (MQ=255)

                                                                                                   aaTTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTc                                     <  1:2837430/140‑1 (MQ=255)

                                                                                                   aaTTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTc                                     <  2:4424084/140‑1 (MQ=255)

                                                                                                     ttATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAg                                   >  2:7547641/1‑140 (MQ=255)

                                                                                                     ttATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAg                                   >  2:4901767/1‑140 (MQ=255)

                                                                                                       aTCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCt                                 >  2:1908069/1‑140 (MQ=255)

                                                                                                        tctcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCtt                                <  1:2682269/140‑1 (MQ=255)

                                                                                                        tctcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCtt                                <  2:713653/140‑1 (MQ=255)

                                                                                                        tctcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCtt                                >  1:5416724/1‑140 (MQ=255)

                                                                                                         ctcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCttt                               >  2:2331428/1‑140 (MQ=255)

                                                                                                         ctcCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCttt                               >  1:512104/1‑140 (MQ=255)

                                                                                                           ccGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAg                             >  2:2559019/1‑140 (MQ=255)

                                                                                                           ccGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAg                             >  2:5748162/1‑140 (MQ=255)

                                                                                                            cGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                            <  2:7727889/140‑1 (MQ=255)

                                                                                                            cGACCATAATGGCTTCCGCAGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCAGCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                            >  1:501333/1‑140 (MQ=255)

                                                                                                              aCCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGcc                          >  2:1209587/1‑140 (MQ=255)

                                                                                                              aCCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGcc                          >  1:6438973/1‑140 (MQ=255)

                                                                                                                    aTGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATAtt                    <  2:3372695/140‑1 (MQ=255)

                                                                                                                      ggCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGa                  <  1:785791/140‑1 (MQ=255)

                                                                                                                      ggCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGa                  <  1:4495749/140‑1 (MQ=255)

                                                                                                                      ggCGTCCGCCGGCTCGCGTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATACGCCGATTCCCGTCAGCTTTAGGCCCATATTGa                  <  2:3870917/140‑1 (MQ=255)

                                                                                                                        cTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGt                                      >  2:4152050/1‑118 (MQ=255)

                                                                                                                         ttCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAg               <  1:4376502/140‑1 (MQ=255)

                                                                                                                         ttCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAg               >  2:6577785/1‑140 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAACAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt              <  2:5294594/140‑1 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTca                                                                                    <  2:3220592/70‑1 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAgg              >  2:4343002/1‑139 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt              >  2:1351305/1‑140 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt              >  1:982861/1‑140 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt              >  2:5398834/1‑140 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt              >  2:731618/1‑140 (MQ=255)

                                                                                                                          tCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGt              >  2:2844231/1‑140 (MQ=255)

                                                                                                                            cgccgGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTAt            <  1:3057406/140‑1 (MQ=255)

                                                                                                                             gccgGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAgg                            <  1:6030804/123‑1 (MQ=255)

                                                                                                                                 gCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCtt       >  2:6285437/1‑140 (MQ=255)

                                                                                                                                 gCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCtt       >  2:3598706/1‑140 (MQ=255)

AATCAGGCTCATGCTTTGCCAGTGTTTCCGGCCCTTTAATCGTCCATGCGACTCCGGCCGTTTTTGTTCCGGCGTTTTTCCCTGCCAGCACATCGTGGTAATTATCTCCGACCATAATGGCTTCCGCCGGCTCGCTTCCTAGCTGTTTCAGCGCTAAAAGCACAGGCTCGGGATCAGGCTTTGCCATCGTCACATCATCAAGGGTAACGACCGTTTCAAAGAATTCGCCGATTCCCGTCAGCTTTAGGCCCATATTGACAGTATCTCTTAATTT  >  NZ_CP034943/3394299‑3394572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`