Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,446,970 G→T 20.8% Q206K (CAA→AAA)  degU ← two‑component system response regulator DegU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,446,9700GT20.8% 847.5 / 90.6 467Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
Reads supporting (aligned to +/- strand):  ref base G (132/224);  new base T (106/3);  total (240/227)
Fisher's exact test for biased strand distribution p-value = 2.86e-33
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 477 total aligned reads displayed.

CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108
                                                                                                                                           |                                                                                                                                          
 tATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGc                                                                                                                                           >  2:3015694/1‑140 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:3933109/140‑1 (MQ=255)
  aTTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  2:1720462/140‑1 (MQ=255)
   ttGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCaa                                                                                                                                         >  2:5002596/1‑140 (MQ=255)
    tGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTAGTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaaa                                                                                                                                        <  2:623613/140‑1 (MQ=255)
    tGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCACTAAAAGGCAATTCTATTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCaaa                                                                                                                                        <  2:4129622/140‑1 (MQ=255)
        aGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAatat                                                                                                                                    <  1:7242858/140‑1 (MQ=255)
         gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATAtt                                                                                                                                   <  2:5393862/140‑1 (MQ=255)
         gggAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATAtt                                                                                                                                   >  2:269545/1‑140 (MQ=255)
           gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCACGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTGTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTGCAAAAAATTGc                                                                                                                                 >  2:73647/1‑140 (MQ=255)
           gAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGc                                                                                                                                 >  2:2213655/1‑140 (MQ=255)
              aCGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTca                                                                                                                              >  1:4830709/1‑140 (MQ=255)
                ggCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                            <  1:7310709/140‑1 (MQ=255)
                    gCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGt                                                                                                                        >  2:2813218/1‑140 (MQ=255)
                     ccTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGtt                                                                                                                       >  1:6710183/1‑140 (MQ=255)
                           aaaaGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAc                                                                                                                 <  1:2474025/140‑1 (MQ=255)
                               gACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGGTCACATGGTTTTTCACGGtt                                                                                                             >  2:6091873/1‑140 (MQ=255)
                                   tGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTtct                                                                                                         <  1:3261431/140‑1 (MQ=255)
                                     cccAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCa                                                                                                       <  2:4320906/140‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCGAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGTTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  2:4427726/140‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  1:4221637/140‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGa                                                                                         <  1:6540003/140‑1 (MQ=255)
                                                   aGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAAATTTTTTCAAAAAATTTCTCACATGGTTTTTCAACGTTTTATCACTGATAAACAATGa                                                                                         >  1:7138564/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTGCTGACATGGTTTTTCACGGTTTTATCACTGATAAACAATGAt                                                                                        >  2:4741930/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGAt                                                                                        >  2:1440144/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACAAGGTTTTTCAAGCTTTTCTCACTGATAAACAATTAt                                                                                        >  2:5340947/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTGCTCACATGGATTTTAACGGTTTTATAACTTATAAACAATGAt                                                                                        >  2:5487660/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTTCAAAATATTTCTCACAAGGTTTTTAACGGTTTTCTCACTGATAAACAAAGAt                                                                                        >  1:7664870/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCATTTTTTTCAAAATATTTCTCACAAGGTTTTTCACGGTTTTATCACTGATAAACAATTAt                                                                                        >  1:1490531/1‑140 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTAAATTAATTTTTTTCAAAATATTTCTCAAAAGGTTTTTAACGGTTTTATAAATTATaaaaaaatat                                                                                        >  1:2070161/1‑136 (MQ=255)
                                                    gTCTCTTATTATACTATCTCATATCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACAATCATTTTTTGCAAAATATTGCGCACATGGTTTTTCACGGTTTTATCACTGATAAACAATTAt                                                                                        >  2:3280549/1‑140 (MQ=255)
                                                              ataCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATAc                                                                              <  1:589698/140‑1 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGCAAAATATTGCTCACATGGTTTTTCACCGTTTTCTCACTGATAAAAAATGATTCAACAAAAcc                                                                             >  1:4676572/1‑140 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAAATTTTTTCAAAATATTTCTCACATTGTTTTTAAAAGTTTTATAACTTATAAAAAAATATTAACCAAAAcc                                                                             >  1:7013590/1‑140 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAAATTTTTGAAAAAAATTTCTAAAATTGTTTTTAAAGGTTTTATAACTTATAAAAAAATAATCACCaaaaac                                                                             >  1:103021/1‑136 (MQ=255)
                                                               taCTATCTCATTTCTACCCAGCCATTTTTAATGGCGAACACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTAAAAATAATTCTCACAGGGTTTTTCACAGTTTTATCACTGATACACAAAGATTCACCAATAcc                                                                             >  2:2346276/1‑140 (MQ=255)
                                                                aCTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCAAATTTTGCAAAAAAATGCTCACCAGGTTTTTAAAGGTTTTATCACAGAAAAAAAAAGATTCACAAAAAcac                                                                            >  2:1951517/1‑138 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATATTTATCACAATGTTTTTCACGGTTTTATCACTGATAAAAAAATATTCACCAAAAAcgc                                                                           >  2:6856818/1‑140 (MQ=255)
                                                                 cTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCAAGGTTTTCTCACATAAAAACAATGAATCAACAATACcgc                                                                           >  2:5236908/1‑140 (MQ=255)
                                                                  tATCTCATGTCTACCAAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACcgcg                                                                          <  2:1814449/140‑1 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCATTCATTTTTTTCAAAATATTGCTACCATGGTTTTTCAAGGTTTTCTCACTGATAAACCCTGATTCACCAATAACGCgg                                                                         >  2:205664/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCAATAATTTTTTTCAAAAAATTTATCAAATTGTTTTTAACGGTTTTATCACTTATAAAAAAATATTAACAAATAACGCgg                                                                         >  1:1706861/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTTCAATAAATTTTTTCAAAATATTTGTCAAAAGGTTTTTAACGGTGTTATCACTGATAAACAATTATTCACCAATAACCCgg                                                                         >  1:3646278/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTAAAAATATTGCTCACAAGGTTTTTAAAGGTTTTCTCACTGATAAACAATTATTCACCAATAACGCgg                                                                         >  2:4943049/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCAAATGGTTTTTCACCGTTTTCTCACTGATAAACAATGATTAACCCAAAcccccg                                                                         >  2:912209/1‑136 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTTCAAAAAATTTCTAAAATGGTTTTTCACGGTTTTATCACAGATAAACAAAGATTCAACAAAACCCCgg                                                                         >  1:1204153/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTCAAAATATTTCTCACATGGTTTTTAACGGTTTTGTGACATATAAACAATGATTAAACAAAAACGCgg                                                                         >  1:5564833/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGGAAAATATTTCACACATGGTTTTTCACGGTTTTGTGACAGATAAACAATGATTCACCACAAACGCgg                                                                         >  1:2253149/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTGCAAAATAATGAACACATGGTTTTTCAAGGTTTTCTCACTGATAAACAAAGATTAAACAATAACCCgg                                                                         >  2:7743888/1‑140 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTTGAAAAAATTGCTCACATGGTTTTTAAAGGTTTTTTCACTTATAAAAAATAATTCAACAATAcaccgg                                                                         >  1:462195/1‑135 (MQ=255)
                                                                   aTCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACCTTCAATTTTTGGAAAAAATTGCTCACAAGGTTTTTAACGGGTTTATCACAGATAAACAAAGATTCACCCAAAACGCgg                                                                         >  1:960003/1‑140 (MQ=255)
                                                                   aTCACATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACCTGGGTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCgg                                                                         >  2:7026670/1‑140 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCa                                                                                                                                          <  1:3174932/74‑1 (MQ=255)
                                                                    tctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTAAAAATATTTCTAAAATGTTTTTTAAAGGTTTTATCACTGATAAAAAAAGATTAAAAAAAACCGCGGt                                                                        >  2:6569758/1‑140 (MQ=255)
                                                                     ctcATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTACATTAATTTTTTTAAAAATATTTCTCACATGGTTTTTCACCGTTTTATGCCTGATAAAAAAATATTCAACAATACCCCGGtt                                                                       >  1:5069523/1‑140 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTcaca                                                                                                                            <  1:7355994/83‑1 (MQ=255)
                                                                         tttCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAATTAATTTTTTTAAAAATATTTCTCACATTGTTTTTAAAGGTTTTATCACTTATAAACAATTATTCACCAATACCGCGGTTGCtt                                                                   >  2:6749905/1‑140 (MQ=255)
                                                                            cTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCAATTTTTGACAAAAATTGCTCACATGGTTTTTCACGCTTATATCACTGATAAACAATTAATAAACAATAACGCGCTTGCtttat                                                                >  2:5917371/1‑138 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTAAAATCATTTTTTGCAAAATATTTCTCACATTGTTTTTGACGGTTTTATAAAAGATAAACAAATATTACACAATAACCCGGTTGCTTTTTca                                                              >  2:4516636/1‑139 (MQ=255)
                                                                              aCCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTGTTCATTAATTTTTTTCAAAAAATTTCTCACAAGGTTTTTCACGGTTTTGTCACTGATAAACAAATATTAACCAAAACCCCGGTTGGttttttc                                                              >  1:3558299/1‑138 (MQ=255)
                                                                                   gCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTAATTTTTTTGAAAAAATTGCTCACATTGTTTTTCACGGTTTTTTCACTGAGAAAAACATATTCACCAAAACCGCGGGTGCTTTTTGCGGCTg                                                         >  1:6346627/1‑140 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  1:7422338/140‑1 (MQ=255)
                                                                                     cATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCa                                                       <  1:1899743/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGc                                                    <  2:7257826/140‑1 (MQ=255)
                                                                                        ttttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGc                                                    <  1:3015694/140‑1 (MQ=255)
                                                                                          ttAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCAt                                                  <  1:2545426/140‑1 (MQ=255)
                                                                                              tGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgc                                              >  2:5624356/1‑140 (MQ=255)
                                                                                                 cGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGGTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCCAAACCGCGGTTGCGTTTTCCGGATGCAACCATCAtcagc                                           >  2:3938907/1‑140 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTGAAAATATTGCTCACATGGTTTTTCACGGTTTTGTCACTGATAAAAAATGATTGAACAAAACCGCGGTTGCTTTTTGCGTCTGCAAGCATCGgcagca                                          >  1:5365061/1‑140 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTTCAAAATAATGCTCACATTGTTTTTCAAGGTTTTGTCACTGATAAACAATGATTAACCAAAACCGCGGTTTCTTTTTGCGTGTGCAAGCATATggagca                                          >  1:5691148/1‑140 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                          <  1:3346684/140‑1 (MQ=255)
                                                                                                  gACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTgcagca                                          <  1:6101005/140‑1 (MQ=255)
                                                                                                   aCCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATTATTCTCCAATACCGCGGTTTGTTTTTCCGTCTGCAAGCATCTGCAGCAc                                         >  2:4651527/1‑140 (MQ=255)
                                                                                                     cacaACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACtt                                       <  2:7451912/140‑1 (MQ=255)
                                                                                                       ccaCGGCGTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTca                                     <  2:4829608/138‑1 (MQ=255)
                                                                                                             cTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTTCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTACGGCTGCAAGCATCTGCAGCACTTCACATTcc                               >  2:4638775/1‑140 (MQ=255)
                                                                                                                gCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgc                            <  1:6003639/140‑1 (MQ=255)
                                                                                                                 cGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGGTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCccgcc                           <  2:3608948/140‑1 (MQ=255)
                                                                                                                     cGGTCGTTTACATTCATTTTTTGCAAAATAATGCTCACATTGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCCGTTGCTTTTTCCGTCTGCAAGCATCAGCAGCACTTCAAATTACCGCCGAGt                       >  2:1257418/1‑140 (MQ=255)
                                                                                                                      ggTCGTTTACATTCAATTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGtt                      <  2:6019440/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGGTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCTCCAATACCGCGGGTGCTTTTTCCGTATGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  2:5133648/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:3752368/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:1778024/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:2739550/140‑1 (MQ=255)
                                                                                                                         cGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTaaa                   <  1:1348285/140‑1 (MQ=255)
                                                                                                                         agTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaa                   <  1:7756366/139‑1 (MQ=255)
                                                                                                                          gTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTaaaa                  <  1:4357565/140‑1 (MQ=255)
                                                                                                                           tttACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAat                 <  2:2452666/140‑1 (MQ=255)
                                                                                                                                attcattTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTa            <  2:7419477/140‑1 (MQ=255)
                                                                                                                                        tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTg    <  2:7138564/140‑1 (MQ=255)
                                                                                                                                        tttGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTg    <  1:549258/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACTTGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:5715890/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:5240717/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:6432107/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:684549/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:2865579/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:1138612/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  >  2:757903/1‑140 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  1:4113095/140‑1 (MQ=255)
                                                                                                                                          tGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTGTCCGTCTGCAAGCATCTGCAGCACTTCACAGTCCCGCCGAGTTAAAATATGTAATGGTCTGCg  <  2:5809197/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTATTGTAAGGGAAACGGCAGCCTAAAAAAAGACCTGCCCAGTAAAAGGCAAGTCTCTTATTATACTATCTCATTTCTACCCAGCCATTTTTAATGGCGACCACAACGGCTTGCGTCCGGTCGTTTACATTCATTTTTTGCAAAATATTGCTCACATGGTTTTTCACGGTTTTCTCACTGATAAACAATGATTCACCAATACCGCGGTTGCTTTTTCCGTCTGCAAGCATCTGCAGCACTTCACATTCCCGCCGAGTTAAAATATGTAATGGTCTGCG  >  NZ_CP034943/3446831‑3447108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: