Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,562,015 A→C 7.8% intergenic (‑661/‑137) EO946_RS18300 ← / → EO946_RS21105 response regulator/HAMP domain‑containing histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,562,0150AC 7.8% 1056.6 / 6.5 419intergenic (‑661/‑137)EO946_RS18300/EO946_RS21105response regulator/HAMP domain‑containing histidine kinase
Reads supporting (aligned to +/- strand):  ref base A (245/111);  new base C (4/53);  total (249/170)
Fisher's exact test for biased strand distribution p-value = 1.28e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 421 total aligned reads displayed.

ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATC  >  NZ_CP034943/3561879‑3562151
                                                                                                                                        |                                                                                                                                         
aTCAGCACGTCTTTTATTTACAGTGCAAATCGCATCTATTTTAATGGACAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAggg                                                                                                                                       <  2:2488288/140‑1 (MQ=255)
           gtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAAc                                                                                                                            <  2:4138490/140‑1 (MQ=255)
           gtTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAAc                                                                                                                            <  2:2627872/140‑1 (MQ=255)
              tatttaCAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTtg                                                                                                                         <  1:4216396/140‑1 (MQ=255)
               atttaCAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTtgt                                                                                                                        >  1:1383841/1‑140 (MQ=255)
                    cAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAc                                                                                                                   <  1:349542/140‑1 (MQ=255)
                    cAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAc                                                                                                                   <  2:7743610/140‑1 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:7705951/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  2:3722149/1‑140 (MQ=255)
                      gTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACaa                                                                                                                 >  1:5262744/1‑140 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATATCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                                                                >  2:4005849/1‑140 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                                                                >  2:7125342/1‑140 (MQ=255)
                       tGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTCTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAt                                                                                                                >  1:5474723/1‑140 (MQ=255)
                         cAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTtg                                                                                                                         >  2:4578634/1‑129 (MQ=255)
                         cAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAAttt                                                                                                              >  2:1389974/1‑140 (MQ=255)
                            aTCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCa                                                                                                           >  2:7090547/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCCATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACATGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAAATTGTGGTAGACTTTTCAAACtt                                                                                                      >  1:1817029/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAAGCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAATCtt                                                                                                      >  1:4519277/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                      >  1:3024640/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGGGGTACAATTTTCAAACtt                                                                                                      >  1:5981621/1‑140 (MQ=255)
                                 aTCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGAGTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACtt                                                                                                      >  1:616272/1‑140 (MQ=255)
                                      ttttAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGaa                                                                                                 <  2:10328/140‑1 (MQ=255)
                                       tttAATGGCCAATTAATAGCACAAAGCGGAAGCCCTTCAAATAAACATGATTTTATAGCGAAAAACACACCATATACGACCTTTTTTACGCTTCACAAGGTTTACAATGAACTTGTGGTACAATTTTCAAACTTCGGaaa                                                                                                <  2:2381655/140‑1 (MQ=255)
                                           aTGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGAGCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACTATTTTCAAACTTCGGAAAAACg                                                                                            >  2:7426589/1‑140 (MQ=255)
                                            tGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGc                                                                                           <  2:1290168/140‑1 (MQ=255)
                                            tGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACACACCATATACGCGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGc                                                                                           <  2:3305144/140‑1 (MQ=255)
                                              gCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCg                                                                                         >  1:2787371/1‑140 (MQ=255)
                                                      aTAGGACAAAGCGGAACCCCTTCAAATAAACCTGATTTTATTGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTgggg                                                                                 <  2:5323560/140‑1 (MQ=255)
                                                        aGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTAGGAAAAACGCCGGGGTGGGGaa                                                                               >  2:6108536/1‑140 (MQ=255)
                                                         ggACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTGTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAATAACGCCGGGGTGGGGAAt                                                                              >  1:6969975/1‑140 (MQ=255)
                                                          gACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATc                                                                             >  2:6411877/1‑140 (MQ=255)
                                                             aaaGCGCAAGCCCTTAAAAAAAACGTGTTTTTATAGCGAAAAACACACCATATACGAGGTTTTTTACGCTTCACACGGGTGACAATGAAGTTGTGGTAAAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCa                                                                          <  1:6402823/140‑1 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACTCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGTACTCGaaaa                                                                        >  1:3196252/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACACTTTTCACACTTCGGAAAAACGCCGGGGGGGGGAATCGCaaa                                                                        >  2:4375195/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                        >  1:5048754/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                        >  1:3384273/1‑140 (MQ=255)
                                                               aGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCaaa                                                                        >  1:7268342/1‑140 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                   >  2:119885/1‑140 (MQ=255)
                                                                    aaGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGa                                                                   >  1:4613343/1‑140 (MQ=255)
                                                                     cccccTTAAAATAAACCTGTTTTTATAGCCCAAACCACACCATAAACGAGCTTTTTCCCGCTACAACTGGGTCACATTCACCTTGTGTTACATTTTAAAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGAt                                                                  <  1:2607377/138‑1 (MQ=255)
                                                                          ttCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACtt                                                             >  2:3812181/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGCATCGCAAAGCCGATTACttt                                                            >  1:6603562/1‑140 (MQ=255)
                                                                           tCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACttt                                                            >  1:397223/1‑140 (MQ=255)
                                                                             aaaTAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAAGGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTg                                                          <  2:511479/140‑1 (MQ=255)
                                                                             aaaTAAACCTGATTTTATAGCGCAAAACACACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTg                                                          <  2:736679/140‑1 (MQ=255)
                                                                                tAAACCTGTTTTTATCGCGAAAAACACACCATCGAGGAGCTTTTTTACTCTTACAACCTGTGACAATGTAGTTGTGTTACAATTTTCACAATTCGTAACAACGCCGGGGTGGGGAATCGCAAAGACGATTACTTTTGTaa                                                       <  2:6216578/140‑1 (MQ=255)
                                                                                  accctttttttATAGCGAAAAACACACAATATACGCGCTTTTTTACGCTTCACACGGGTGAAAATTAACTTGTGTTAAAATTTAAAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3267667/133‑1 (MQ=255)
                                                                                  acccTTATTTTATAGCCAAAAACCCACCATATACGCGCTTTTTTACGCTACACACGGGTGACATTGAAGTTGTGGTACAATTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:1075534/138‑1 (MQ=255)
                                                                                  acccTGATTTTATCGCGCAACACACACCATAGACGAGCTTTTTTACGCTTCACACGGGTCACAATCAACTTGTGGTACAATTTACAAACTACGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3814966/138‑1 (MQ=255)
                                                                                  aaactttttttATCGCGAAAAACAGAACATAAAAGTGATTTTTGACGATTCACACGGGTGACAATGAATTTTTGGTACATTTTAAAAACTTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3527793/133‑1 (MQ=255)
                                                                                  aaCCTTTTTTTATAGCCCAAACCACACCATATACGAGCTTTTTTACGCTTCACAAGGTTGACAATGAATTGTTGGTACACTTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3098455/140‑1 (MQ=255)
                                                                                  aaCCTTTTTTTATAGCCAAAAACCCACCTTATACGCGATTTTTTACGCTACACACGGGTGACAATGAACTTGTGGTACAATTTTCAACATTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:3658935/140‑1 (MQ=255)
                                                                                  aaCCTTATTTTATAGCGCAAAACACCCCATATCCGCGCTTTTTTACGCTTCACACGGGTCACAATGAAGTTGTGGTAAAATTTCCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:6126416/140‑1 (MQ=255)
                                                                                  aaCCTTATTTTATAGCGCAAAACACACCATATACGCGATTTTTTACGCTTCACACGGGTGACATTGAACTTGTGTTACAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  2:5476967/140‑1 (MQ=255)
                                                                                  aaCCTGTTTTTATAGCGCAAAACCCACAATATACGAGCTTTTTTACGCTTCAACAGGGTGACAATGACCTTGTGTTACAATTTTCAACCTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:5124824/140‑1 (MQ=255)
                                                                                  aaCCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGACTCGCAAAGCCGATTACTTTTGGAACg                                                     >  1:3618622/1‑140 (MQ=255)
                                                                                  aaCCTGATTTTATAGCCCAAAACACACCATATAGGACTTTTTTTACGCTTCACACGGGTGACAATGAAGTTGGGGTAAAATTTTCAAACTCCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACg                                                     <  1:6282513/140‑1 (MQ=255)
                                                                                   aCCTGATTTTATTGCGCAAAACACACAATATACGAGCTTTTTTCCGCTTCACAAGGGTGACAATGAACTTGTGGTAAATTTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                                                    <  1:1566459/140‑1 (MQ=255)
                                                                                   aCCTGATTTTATACCCCAAACCCCACCTTATCCGCGTTTTTTCCCGCTTCACCCGGGTGACAAGCACGTTGGGGTACAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGc                                                    <  1:673478/140‑1 (MQ=255)
                                                                                    ccTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCa                                                   >  2:1561419/1‑140 (MQ=255)
                                                                                      tttttttATAGCGAAAAACAGACAATATACGAGATTTTTTACGCTTCACACGGGTGAAATTTAACTTGTGGTACAATTTTAAAACTGCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                 <  1:5834667/137‑1 (MQ=255)
                                                                                      ttaTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACACGGGTGACAATGAAATTGTGGTACAATTTTCAAAATTCGTAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAgg                                                 <  2:4921533/138‑1 (MQ=255)
                                                                                       taTTTTATAGCGAAAAACAGACCATTGACGCGCTTTTTTCCGTTTCACATGGGTGACAATTAACTTTTGTTACAATTTTCAAACTTCGTAAACACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGa                                                <  2:88814/139‑1 (MQ=255)
                                                                                       gATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGTAACGCAGGa                                                >  1:6857828/1‑140 (MQ=255)
                                                                                       gATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGa                                                >  1:4985972/1‑140 (MQ=255)
                                                                                        aTTTTATAGCGCAAACCAGCCCATATACGAGCTTTTTTACGCTTCACCAGGGTGACAATGAACTTGTGGTACCATTTTCAACCTTGGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGAt                                               <  2:5533968/140‑1 (MQ=255)
                                                                                        aTTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACCCTGAACTTGTGGTACAATTTTCAAACTTCGGACAAACGCCGGGGTGGGGAGTCGCAAAGCGGATTACTGTTGGACCGCAggct                                               >  2:4492947/1‑138 (MQ=255)
                                                                                                   cAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAAGATATTGCg                                    >  2:4386139/1‑140 (MQ=255)
                                                                                                    aacaccgACCATATCCGAGATTTTTTACGCTTCACAAGGGTTAAAATCAACTTTTGGTACATTTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCga                                   <  2:6936245/134‑1 (MQ=255)
                                                                                                        cacaCCATATACTCGCTTTTTTACGCTTCACACGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGCAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCgagaga                               <  1:7354974/137‑1 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  2:3578676/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  1:2143322/1‑140 (MQ=255)
                                                                                                           aCCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACa                            >  1:7398671/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATTAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:2295376/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGaa                                                       <  2:6778821/108‑1 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCTAGAGAACAAAAtt                       >  1:1199592/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:1311814/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:5569516/1‑140 (MQ=255)
                                                                                                                ataCGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCCATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAAtt                       >  1:1492790/1‑140 (MQ=255)
                                                                                                                     aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                  >  1:2554144/1‑140 (MQ=255)
                                                                                                                     aGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCtt                  >  1:4123974/1‑140 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTAGAATTTTCAAACTTCGGAAAAACGGCGGGGTGGGGAATCGCAAAGCCGATTAGTTTTGGAACGCAGGATAACATATTGCGAGAGACGAAAATTTCCTTTc                >  2:7518367/1‑140 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAAc                                                      >  1:7043764/1‑102 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTc                >  1:7185908/1‑140 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTc                >  2:566968/1‑140 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAAAATATTGCGAGAGAACAAAATTTCCTTTc                >  1:4823141/1‑140 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAAAGCCGGGGGGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTc                >  1:5698450/1‑140 (MQ=255)
                                                                                                                       cTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGCTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCCCAAAGCCGATTACTTATGGAAGGCATGATAACATAATGCGAGAGAAAAAAATTTCCTTTc                >  2:3116379/1‑140 (MQ=255)
                                                                                                                         tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt              >  2:2769945/1‑140 (MQ=255)
                                                                                                                         tttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCtt              >  2:7672531/1‑140 (MQ=255)
                                                                                                                          ttttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTa             >  1:2049045/1‑140 (MQ=255)
                                                                                                                           tttACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAACCTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTaa            <  2:180571/140‑1 (MQ=255)
                                                                                                                               cGCTTCACCAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGAt        <  2:5269474/140‑1 (MQ=255)
                                                                                                                                gCTTCACACGGGTGACAATGAAGTTGTGGTACAATTTACAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGAtt       <  1:1731492/140‑1 (MQ=255)
                                                                                                                                    cacaCGGGTTACAATGAACTTGTGTTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   <  2:2143808/140‑1 (MQ=255)
                                                                                                                                    cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   >  1:4374324/1‑140 (MQ=255)
                                                                                                                                    cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   >  1:819261/1‑140 (MQ=255)
                                                                                                                                    cacaAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   >  1:2355693/1‑140 (MQ=255)
                                                                                                                                    cacaAGGGTGAAATTGAAGTTTTGGTACATTTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAAt   <  2:6342573/140‑1 (MQ=255)
                                                                                                                                     acaAGGGTTACAATTAACTTGTGGTAAAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATc  <  2:5048754/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                         
ATCAGCACGTCGTTTATTTACAGTGCAAATCGCATCTATTTTAATGGCCAATTAATAGGACAAAGCGGAAGCCCTTCAAATAAACCTGATTTTATAGCGCAAAACAGACCATATACGAGCTTTTTTACGCTTCACAAGGGTGACAATGAACTTGTGGTACAATTTTCAAACTTCGGAAAAACGCCGGGGTGGGGAATCGCAAAGCCGATTACTTTTGGAACGCAGGATAACATATTGCGAGAGAACAAAATTTCCTTTCTTAATGATTCAATC  >  NZ_CP034943/3561879‑3562151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: