BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,193	G→T	5.0%	N113K (AAC→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,193	0	G	T	5.0%	1514.6 / 5.2	689	N113K (AAC→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (305/304); new base T (56/3); total (382/307)
Fisher's exact test for biased strand distribution p-value = 7.28e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 694 total aligned reads displayed.

CTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTA  >  NZ_CP034943/240054‑240332

   tggtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTGAAGttt                                                                                                                                          >  2:2588059/1‑140 (MQ=255)

    ggtggAGAAGGTGTACGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCTACTATACGGGCGTTTTCTGATTTTTGTTTTAACtttt                                                                                                                                         >  1:5754888/1‑140 (MQ=255)

       ggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTggg                                                                                                                                      >  1:2004081/1‑140 (MQ=255)

             ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGAttt                                                                                                                                >  1:4907237/1‑140 (MQ=255)

              gtgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTg                                                                                                                               <  1:3059358/140‑1 (MQ=255)

                gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTTaa                                                                                                                             >  2:252807/1‑140 (MQ=255)

                gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                             <  2:2901672/140‑1 (MQ=255)

                  aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGga                                                                                                                                     <  2:3886478/130‑1 (MQ=255)

                  aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATg                                                                                                                           >  2:460719/1‑140 (MQ=255)

                       agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACTGGCGTTTTCGGAATTTTTTTTTAAGTTTTGTGAGATTTGAATGGCttt                                                                                                                      >  2:2025480/1‑140 (MQ=255)

                       agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCttt                                                                                                                      >  2:32264/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATATGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                    >  1:1217368/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGGtttta                                                                                                                    >  1:2931281/1‑139 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGCtttta                                                                                                                    >  1:2134327/1‑139 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATTGCTTTTc                                                                                                                    >  1:6444587/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAAAGGCTTTTc                                                                                                                    >  2:1875468/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAACTTTTTGGAGATTTGAATTGGTTTTc                                                                                                                    >  1:1009165/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCtttta                                                                                                                    >  1:5438746/1‑139 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                    >  1:1413411/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGTGACATTTGAATTGGttttg                                                                                                                    >  1:3953367/1‑139 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGGAGTGTGGGGATGTTTGAATGGCTTTTc                                                                                                                    >  2:4172386/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATTGCTTTTc                                                                                                                    >  1:7653951/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                    >  1:7747328/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATGGCTTTTc                                                                                                                    >  1:3553159/1‑140 (MQ=255)

                         agagaAGGAGCATCTCTGATGGTGGAATTATTCAGAGATGCTGTTTGTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGCCTATACGGGCGGTTTCGGATTTTTGTTTTCAGTTTTGGGTGATTTGAATGGGTTTTc                                                                                                                    >  1:6680266/1‑140 (MQ=255)

                                     tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGTATGGCTTTTCCGTTTTTAAAAc                                                                                                        >  2:3100160/1‑140 (MQ=255)

                                      ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                       <  1:2388509/140‑1 (MQ=255)

                                           aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTTGGAGATTTGAATGGCTTTTACGTTTTTAaaaaaaaatg                                                                                                  >  1:85440/1‑136 (MQ=255)

                                               tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCGAATACGAAGTCGATCATTCCGACTATCCGGGCGTTTTCGGATTTTTGTTTCCAGTTTTGGGAGATATGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                              <  2:5945252/140‑1 (MQ=255)

                                                ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGTTCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTGCGTTTTTAAAAAAAGAGGCAAGc                                                                                             >  1:3650768/1‑140 (MQ=255)

                                                ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGGTTTTACGTTTTTTAAAAAAAAGGCAAGc                                                                                             >  1:6012247/1‑140 (MQ=255)

                                                ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                                             >  2:135544/1‑140 (MQ=255)

                                                ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGTATTTTTGGGAGATTTTAATTGCTTTTTCGTTTTTTAAAAAATAGGCAAGc                                                                                             >  1:3442052/1‑140 (MQ=255)

                                                   attattCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTGGAGATTTTTATTGCTTTTCCGTTTTTTAAACAAGAGGCAAGCCac                                                                                          >  1:584047/1‑139 (MQ=255)

                                                      cttCAGAGATGCTGTTTTTGTATATTTGTTCCAATACGAAGTCGATCCTTCCGACGATACGGGCGTTTTCGGATTTTGGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                       <  2:5842887/139‑1 (MQ=255)

                                                      attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                       <  1:5202905/140‑1 (MQ=255)

                                                      attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAAGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                       >  2:5092572/1‑140 (MQ=255)

                                                       ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGTTTTGAATTGCTTTTACGTTTTTAAAAAAAAAGGCAAGCCAGATaa                                                                                      >  1:3771282/1‑140 (MQ=255)

                                                       ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATaa                                                                                      >  2:3319994/1‑140 (MQ=255)

                                                            agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGGTTTTACGTTTTTAAAAAAAGAGGCAAGCCACAGAAAaccc                                                                                 >  1:6249097/1‑139 (MQ=255)

                                                            agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTGGGGGATTTGAATGTGTTTTGAGTTTTTTAAAAAAGAGGCAAAGCAGAGAAAACCg                                                                                 >  1:2806092/1‑140 (MQ=255)

                                                            agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGTAGTTTTGGGAGATTTTAATGGCTTTTACGTTTTTAAAACAAAAGGCAAGCCCGAAAAAcccc                                                                                 >  1:7161571/1‑139 (MQ=255)

                                                                gCTGTTTTTGTATTTTTGTTCTAATCCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTGTGAAGTTTTGGGAGATTGGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaa                                                                             <  2:4912446/140‑1 (MQ=255)

                                                                gCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaa                                                                             <  1:6118789/140‑1 (MQ=255)

                                                                 cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGATATTTGAATGTCTTTTACGTTTTTAAAAAAAAAGGCAAGCCAGATAAACCCGacaaa                                                                            >  2:4752169/1‑139 (MQ=255)

                                                                 cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATTTCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAACCCGacaac                                                                            >  2:7758956/1‑140 (MQ=255)

                                                                 cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAACTTTTGGGATATTTTAATTGCTTTTACCTTTTTAAAAAAAGAGGCAAGCCACAAAAAACCGacaac                                                                            >  1:4132105/1‑140 (MQ=255)

                                                                 cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACAATACGGGCGTTTGCGGATTTTTGTTTGAAATTTTGTTAGAGTTTAAATGATATTCCGTATTTAATACAATATGCAACCCAGAGAAACACGaaaac                                                                            >  2:3012426/1‑140 (MQ=255)

                                                                     ttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTGAAGTTGGGGGCGATTTGAATGGCTGTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaaca                                                                        <  1:889426/140‑1 (MQ=255)

                                                                      tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGTAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAACCACATAAACCCCAcaacaacaa                                                                       >  1:971951/1‑140 (MQ=255)

                                                                      tttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAAAGGCAAGCCAGATAAACCCGAcaccaaaaa                                                                       >  1:5331441/1‑140 (MQ=255)

                                                                      tttGTATTTTTGTTCTAATAATAATTATATAAAACCGACTATACGGTCGTTTTATTATTTTTTTTTTAAATTTTTTGAGATTTTAAAGGCTTTTCAGTTTTTAAATAAATATGCAAAACACATAAACACGaaaaaaaaaa                                                                       >  2:6489861/1‑140 (MQ=255)

                                                                            tttttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGTGATTTGAATTGCGTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACaaaaaaaa                                                                 >  1:1738117/1‑138 (MQ=255)

                                                                                  ttCTAATACGAAGTCGCTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                           <  2:5641273/140‑1 (MQ=255)

                                                                                  ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                           <  2:7259443/140‑1 (MQ=255)

                                                                                  ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                           <  1:113777/140‑1 (MQ=255)

                                                                                       aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAACGGCTTTTCCCTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                      <  1:5625191/140‑1 (MQ=255)

                                                                                        tACGAAGTCTATCATTCCGACTTTACGGGCGTTTTCGGTTTTTTGTTTGATGTTTTGGGAGATTTGAATGGCATTTCCTTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                     <  2:7593952/140‑1 (MQ=255)

                                                                                        tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                     <  2:5454766/140‑1 (MQ=255)

                                                                                        acagAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                     <  1:6944651/137‑1 (MQ=255)

                                                                                          cGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaaa                                                   <  1:2443457/140‑1 (MQ=255)

                                                                                            aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACACGACAACAACAAAAAATAATCTATCAAGTACACt                                                 >  2:4263526/1‑140 (MQ=255)

                                                                                             aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                <  1:3304581/140‑1 (MQ=255)

                                                                                             aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                <  1:1495536/140‑1 (MQ=255)

                                                                                             aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                <  2:6155023/140‑1 (MQ=255)

                                                                                             aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                <  2:6049636/140‑1 (MQ=255)

                                                                                                  aTCATTCCGACTATACGGGCGTTTTCGGATTGTTTTTTGATGTTTTGGGAGGTTTGTATGGGTTTTGCGTTTGTTAAACAAGCGGCAAGCCAGCTAAACCCGACCACAAACGAAAATAATAGAGCACGTCAACTcccggc                                           >  1:7489656/1‑136 (MQ=255)

                                                                                                   tgataccGACTATACGGGCGTGTTCCGATTTTTGTTTGAAGCTTTGCGAGCTGTGAATGGCTTTGCCGTTTGTAAAACAACAGGCAAGCCAGATAAACCCGACAACAACAAAATATACTCTAGCATGTAAACTCcatgca                                          <  2:766351/135‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGATGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  1:1325885/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  1:6364540/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  1:4439139/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  2:3689020/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  2:3489812/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  1:1591911/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  2:7103253/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  2:106510/140‑1 (MQ=255)

                                                                                                   tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  2:1483346/140‑1 (MQ=255)

                                                                                                   tCATTCCGAATATACGGGCGTTTTCGGAATTTAGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACATCAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  2:1695896/140‑1 (MQ=255)

                                                                                                   ccATTCCGACTAGACGGGCGTTTTCGGATTTTTGTTTGACGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                          <  1:262338/139‑1 (MQ=255)

                                                                                                       tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                                      <  2:2749674/140‑1 (MQ=255)

                                                                                                       tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAAAAAACTAGCAACTAAACTCcaagcactgt                                      >  2:6944651/1‑136 (MQ=255)

                                                                                                         cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACAAAAAATAATATAGCAAGTAAACTACATGCATGGGGt                                    >  2:670175/1‑140 (MQ=255)

                                                                                                           aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAACCCACATAAACCCGACCACAACAAAAAAAAATCTATCAAGTAAAATCCATGAATGGGGTaa                                  >  2:7030397/1‑140 (MQ=255)

                                                                                                               taCGGGCGTGTTGGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATGGCGTTTCCGTTTTTAAAACAAGAGGCAACCCAGATAAAACCCCCAACAACAAAAAAAAATAAAGCAAGTAAACACCAAGCAAGGGGGaaataa                              >  1:4191858/1‑136 (MQ=255)

                                                                                                                    gCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCACAAAAACAAAAAATAATATAGCAAGTAAACTCCATGCATTGGGTAAATAATTAGt                         >  2:900285/1‑140 (MQ=255)

                                                                                                                         ttCTGATTTTTGTTTGTAGTTTTGGGATATTTGTATGGATTTTCCGTTTTTAAAACAAGAGGCAATCGATATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATTCATGGGGTAATTCATTCGTTGCaa                    <  1:4606376/140‑1 (MQ=255)

                                                                                                                         ttCTGATTTTTGTTTGAAGTTTTGTGAGATTTGAATTGATTTTCCGTATTTAAAACAAGAGGCAAGCCAGATAAACCCGACAAAAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                    <  2:4451348/140‑1 (MQ=255)

                                                                                                                         ttCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGGTTTTACCTTTTTAAAAAAATAGGCAAGGCATATAAAACCGACAACAACAAAAAATAATATAGCAAGTAAAATACATTCAAGGGGTAATTCAATCGTTGCaa                    >  2:7500663/1‑140 (MQ=255)

                                                                                                                         ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                    <  1:1849230/140‑1 (MQ=255)

                                                                                                                         ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                    <  2:2375521/140‑1 (MQ=255)

                                                                                                                         ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                    <  2:1213377/140‑1 (MQ=255)

                                                                                                                         ttCGGATTTTTGTTGGCAGTTTTGGGAGAATTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                    <  1:4726251/140‑1 (MQ=255)

                                                                                                                             gATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                <  1:7028827/140‑1 (MQ=255)

                                                                                                                                  ttgtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaa           >  2:223714/1‑140 (MQ=255)

                                                                                                                                   tgtttgAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATaaaaa          <  2:6329427/140‑1 (MQ=255)

                                                                                                                                    gtttgAAGTTTTGGTAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCTACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt         <  1:4995570/140‑1 (MQ=255)

                                                                                                                                     tttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATa        <  2:6811264/140‑1 (MQ=255)

                                                                                                                                      ttgAAGTTTTGGGAGATTTGAATGTCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag       <  2:5221361/140‑1 (MQ=255)

                                                                                                                                      ttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag       <  1:2220312/140‑1 (MQ=255)

                                                                                                                                          aGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGAAAGCCAGATAAACCCGACAACAACAAAAAATAATCTATCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGAAAAATATAAAAATAGAGtt   >  1:7578368/1‑140 (MQ=255)

CTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTA  >  NZ_CP034943/240054‑240332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`