Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,584,283 G→T 7.0% D322Y (GAT→TAT)  EO946_RS18420 → N‑acetylneuraminate synthase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,584,2830GT 7.0% 1703.7 / 30.1 647D322Y (GAT→TAT) EO946_RS18420N‑acetylneuraminate synthase family protein
Reads supporting (aligned to +/- strand):  ref base G (300/274);  new base T (2/70);  total (303/344)
Fisher's exact test for biased strand distribution p-value = 2.29e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 690 total aligned reads displayed.

TACGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACG  >  NZ_CP034943/3584146‑3584422
                                                                                                                                         |                                                                                                                                           
tACGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTgatgat                                                                                                                                           >  1:4846156/1‑140 (MQ=255)
 aCGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGAtt                                                                                                                                          <  2:1748481/140‑1 (MQ=255)
  cGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGAttt                                                                                                                                         <  2:313795/140‑1 (MQ=255)
  cGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGAttt                                                                                                                                         >  2:1613369/1‑140 (MQ=255)
  cGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGAttt                                                                                                                                         >  1:200378/1‑140 (MQ=255)
  cGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGAttt                                                                                                                                         <  1:4068586/140‑1 (MQ=255)
          aGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTaa                                                                                                                                 >  1:1367687/1‑140 (MQ=255)
            ccAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAAc                                                                                                                               >  1:3792736/1‑140 (MQ=255)
            ccAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAAc                                                                                                                               >  1:4949164/1‑140 (MQ=255)
              aaCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                             >  2:786953/1‑140 (MQ=255)
              aaCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCa                                                                                                                             >  2:1907710/1‑140 (MQ=255)
               aCCAGAATATAAAAAAAGGAGACATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                            <  1:4306317/140‑1 (MQ=255)
               aCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATTCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                            <  2:1511266/140‑1 (MQ=255)
               aCCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                            <  1:6628307/140‑1 (MQ=255)
               aCCAGAAAATAAAAAAAGGAGAAATCATTACACTGAATAACACACGCTCCAAAAGAGGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAg                                                                                                                            <  1:3084676/140‑1 (MQ=255)
                ccAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGc                                                                                                                           <  2:458533/140‑1 (MQ=255)
                ccAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGc                                                                                                                           <  2:5220310/140‑1 (MQ=255)
                                ggAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGa                                                                                                           >  2:533598/1‑140 (MQ=255)
                                      aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                     <  1:5593765/140‑1 (MQ=255)
                                      aTCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                     <  1:3170635/140‑1 (MQ=255)
                                      aTCATTACACTGAATAAAACAAGCTCCAAAAGATGAGGAAATTAGAATGCATTAATGCCTTTACAAGCCGAGGACTCATTTCCTCAAGTAGCAACTTCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATaaa                                                                                                     <  2:1194664/140‑1 (MQ=255)
                                      aTCATTACAATGAATAAAAAAAGCTCCAAAAGATGCGGAAATTATAATGCATTAATTCCTTTACAAGCCGAGGACTCATTTCCTCAATTATAAACTTCTTATTTATCTTTAAAAAATACCATTACACCCAACGAAATaaa                                                                                                     <  2:6034889/140‑1 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCTGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTTCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         <  2:2918868/140‑1 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGACATAAAAAGTCTAAGAAt                                                                                         >  2:5680567/1‑140 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         >  1:4771739/1‑140 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         >  2:2483127/1‑140 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         >  1:5093454/1‑140 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         >  2:2392125/1‑140 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         >  1:5506151/1‑140 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         <  1:7676453/140‑1 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         >  2:6952954/1‑140 (MQ=255)
                                                  aaTAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAt                                                                                         >  2:6511261/1‑140 (MQ=255)
                                                    tAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCATTACAAGCCGAGGACGCATTTACTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCCACGAAATAAAAAGTCTAAGAATTg                                                                                       <  2:4345646/140‑1 (MQ=255)
                                                       aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTgaga                                                                                    >  2:4106394/1‑140 (MQ=255)
                                                       aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGa                                                                                    >  2:2620149/1‑140 (MQ=255)
                                                       aaCAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTATAAGAATTGCGa                                                                                    >  2:7369383/1‑140 (MQ=255)
                                                          aaGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                 <  1:7728541/140‑1 (MQ=255)
                                                          aaGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTg                                                                                 <  2:7537859/140‑1 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAATAATTGCGATTGCa                                                                               >  2:7255864/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               <  1:1772739/140‑1 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  2:2212565/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  1:4482529/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               <  2:3707544/140‑1 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  2:4873138/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  1:3744404/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  1:5926787/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  1:2669039/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  1:6788193/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  1:6900356/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  1:7126439/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCAATACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  2:716821/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCACAAGTAGCAACTGCTGATTTATAGTTAAACCATCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGAGATTACa                                                                               >  2:4805441/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAATGAGAATGCAGGAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTATCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAGAGTCTAAGAATTGCGATTGCa                                                                               >  1:296568/1‑140 (MQ=255)
                                                            gCTCCAAAAGATGCGGAAAAGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCa                                                                               >  2:3516523/1‑140 (MQ=255)
                                                                cAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGa                                                                           >  2:1081673/1‑140 (MQ=255)
                                                                        gCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAgg                                                                   >  2:4717865/1‑140 (MQ=255)
                                                                                 agaATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCg                                                          >  2:3271411/1‑140 (MQ=255)
                                                                                       cATTAATGCCATTACAACCCGAGGCCGCTTTTCCTCAATTACCAACTGCTTATTTATCTTTAACCCCGCCCATTCCCCCCAAGGAAACACAAAGTCAAAGATGTGCTATTGCAGTGATATGTAGGCTAAAATCGACTCGt                                                    <  2:1285949/140‑1 (MQ=255)
                                                                                          tAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATACAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTg                                                 >  2:2309427/1‑140 (MQ=255)
                                                                                          tAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTg                                                 <  2:4482529/140‑1 (MQ=255)
                                                                                           aaTGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGc                                                >  2:912519/1‑140 (MQ=255)
                                                                                                 tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaa                                          >  1:7080625/1‑140 (MQ=255)
                                                                                                 tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATATACTCGTCTTCCGaaaa                                          >  1:2315187/1‑140 (MQ=255)
                                                                                                 tttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACACAACGAAATAAAAAGTCTAAGAATTGCGATAGCAGTGATATGTAGGCTAAAATCGAATCTTCTGCCGaaca                                          >  2:6247070/1‑138 (MQ=255)
                                                                                                  ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                         >  2:1016925/1‑140 (MQ=255)
                                                                                                  ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGaaaaa                                         >  1:5757266/1‑140 (MQ=255)
                                                                                                  ttACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCAATACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTTCAGTGATATGTAGGCTAAAATCGACACGTCTGCCGaaaaa                                         >  2:3828867/1‑140 (MQ=255)
                                                                                                      aaGCCGCGGACGCATTTCCTCAAGTAGAAACTGCTGATTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                     <  2:6788193/140‑1 (MQ=255)
                                                                                                      aaGCCGAGGACGTTTTTCCTCATGTAGCAAATGCTGATTTATGGTTAAACCAGCACATTACACCCAACGAAATAAAAAGTCTTATAATTGGGATTGCTGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                     <  2:5798892/140‑1 (MQ=255)
                                                                                                      aaGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAgcg                                     <  2:5529091/140‑1 (MQ=255)
                                                                                                         ccGAGGACGCTTTTCCTCAAGTAGCACCTGCTGATTTTTCGTTAAACCCCCCCATCCCACCAACCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTTATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctg                                  <  1:1054263/140‑1 (MQ=255)
                                                                                                          cGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCgctgc                                 >  1:797318/1‑140 (MQ=255)
                                                                                                            aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                               <  2:7080625/140‑1 (MQ=255)
                                                                                                            aGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                               >  2:7658019/1‑140 (MQ=255)
                                                                                                             gtaCGCTTTTCCTCAAGTCCCAACTGCTTATTTATCGTTAAACCAGCCAATTACACCCAACGAAATAAAAAGTCTAAGATTTGCGATTGCAGTGATTTGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                              <  1:3551887/138‑1 (MQ=255)
                                                                                                             ggACGCTTTTCCTAAAGTAGCAACTGCTTATTTATCGTTAAACCCGCCCATTACACCCACCCAAATAAAAATTCTAACATTTGCGTTTGCAGTTATATTTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTcc                              <  1:7448853/140‑1 (MQ=255)
                                                                                                                 tcATTTCCTAAAGTAGAAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                          <  2:2009167/139‑1 (MQ=255)
                                                                                                                 gCATTTCCTCAATTAGCAACTGCTTATTTATCTTTAAACCACCCCATTACACCCAACGAATTAAAAAGTCTAACAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                          <  2:2807299/140‑1 (MQ=255)
                                                                                                                 gCATTTCCTCAAGTAGCAACTGCTTATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCGGCTCCCTAt                          <  1:6141746/140‑1 (MQ=255)
                                                                                                                 gCATTTCCACAAGTAGCACCTGCTGTTTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCAAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAt                          <  1:215608/140‑1 (MQ=255)
                                                                                                                  cATTTCCTAAAGTAGCACGTGCTGATTTATCTTTAAACCCGCCCATTACACCCAACCAAATAAAAAGTCTAAGAATGGCGATTGCAGTGATAGGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTAtt                         <  1:3926352/140‑1 (MQ=255)
                                                                                                                    tttCCTCAAGTAGCAACTGCTTATTTATCTTTAACCCACCCCCTTACCCCCAACGAAATAAAAAGTCTAAGAATGCCGTTTGCAGTCATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                       <  1:1130006/140‑1 (MQ=255)
                                                                                                                    tttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                       <  2:4949164/140‑1 (MQ=255)
                                                                                                                    tttCCTAAAGTAGCAACTGCTGATTTATCGTTAAACCCGCCCATTACACCCAACGAAATAAAAAGTCTAAGAAGGGCGATTGCAGTGATAGGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                       <  1:6344511/140‑1 (MQ=255)
                                                                                                                    ttgagtCAAGTATCAACTGCTTTTTTATCTTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAATATTTGGTATTGAAGTGATTTGTTGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTa                       <  1:3443204/135‑1 (MQ=255)
                                                                                                                     ttCCTCATGTAGCAAGTGCTTTTTTGTCTTTAAACCAGCCAATTACACCCAACTAAAAAAAAAGTTTAAGAATTGCGATTGCAGTTATATGTCGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                      <  1:416842/140‑1 (MQ=255)
                                                                                                                     ttCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCAAACTAAATAAAACGTCAACTAATTGCGATTGCCTTGATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                      <  2:5421533/140‑1 (MQ=255)
                                                                                                                     taccTCAATTAGCAACTGCTGATTTATCGTTAAACCCGCCCAGTACACCCAACCAAATAAAAAGTCTAAGAATGGCTATTGCAGTGAAATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTAc                      <  1:3313898/138‑1 (MQ=255)
                                                                                                                         tCATGTAGCAACTGCTGTTTTTTCGTTAAACCAGCCCATTACACCCACCGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATTTGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGta                  <  1:1537940/140‑1 (MQ=255)
                                                                                                                             ttAGCAACTGCTTATTTATCGTTAAACAAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCTATTGAAGTTATATGTAGGATAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATAcc              <  2:4148635/139‑1 (MQ=255)
                                                                                                                               agaaagtgctgATTTATGGTTAAACCAGCCCAGTACACCCAACTAATTAAAAAGTCTAAGAATTGGGTTTGCAGTGATATGTAGGCTAAAATCGACTAGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt            <  2:6693307/134‑1 (MQ=255)
                                                                                                                               agaaaCTGCTGATTTATCTTTAAACCCGCCAATTACACCCAACGAAATAAAAAGTCTAAGACTTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt            <  1:7211189/137‑1 (MQ=255)
                                                                                                                               aGCAACTGCTGATTTATCGTTAAACCCGCCAATTACACCCAACGAAATAAAAAGTCTAAGACTTGCGATTGCAGTGATATGTAGGCTAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCAt            <  2:3067403/140‑1 (MQ=255)
                                                                                                                                 cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAGATCGACTCGTCTGCCGAAAAAAGCGCTGCTc                               >  1:1029250/1‑119 (MQ=255)
                                                                                                                                 cAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCt          >  2:7460417/1‑140 (MQ=255)
                                                                                                                                    ctgcttATTTAACGATAAACCACCCCATTACCCCCAACGAAATAAAAAGTCTACGAAGTGCGATGGCAGTTATATGTAGGATAAACTCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGtt       <  1:2434417/140‑1 (MQ=255)
                                                                                                                                       ctgATTTATCTTTAAACCACCCCATTACCCCCAACCAAATAAAACGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa    <  1:533598/140‑1 (MQ=255)
                                                                                                                                       ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa    <  2:6900356/140‑1 (MQ=255)
                                                                                                                                       ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa    >  1:1267830/1‑140 (MQ=255)
                                                                                                                                       ctgATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGaa    <  2:3405835/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TACGTGTGACAGCCAACCAGAATATAAAAAAAGGAGAAATCATTACACTGAATAAAACAAGCTCCAAAAGATGCGGAAATGAGAATGCATTAATGCCTTTACAAGCCGAGGACGCATTTCCTCAAGTAGCAACTGCTGATTTATCGTTAAACCAGCCCATTACACCCAACGAAATAAAAAGTCTAAGAATTGCGATTGCAGTGATATGTAGGCTAAAATCGACTCGTCTGCCGAAAAAAGCGCTGCTCCCTATTTACGGTATACCATCTGTTGAACG  >  NZ_CP034943/3584146‑3584422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: