Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,635,983 G→C 16.4% D171E (GAC→GAG EO946_RS18730 ← fructose‑bisphosphate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,635,9830GC16.4% 1649.1 / 282.6 577D171E (GAC→GAGEO946_RS18730fructose‑bisphosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (231/249);  new base C (51/44);  total (284/293)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 587 total aligned reads displayed.

AGCAGTCGGAATTCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGAAATGAGCAAGCT  >  NZ_CP034943/3635845‑3636122
                                                                                                                                          |                                                                                                                                           
   agTCGGAATTCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGa                                                                                                                                         <  2:2724553/140‑1 (MQ=255)
   agTCGGAATTCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCACGTGCAAGGCAGTCGa                                                                                                                                         <  2:7072104/140‑1 (MQ=255)
            tCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTgc                                                                                                                                <  1:3387461/140‑1 (MQ=255)
            tCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCACTCGATGCCCGTgc                                                                                                                                >  1:6481568/1‑140 (MQ=255)
             ccAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTgcg                                                                                                                               <  2:39997/140‑1 (MQ=255)
                   ccgccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTcaac                                                                                                                         >  2:3159279/1‑140 (MQ=255)
                   ccgccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTcaac                                                                                                                         >  2:5724610/1‑140 (MQ=255)
                     gccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTcaacaa                                                                                                                       <  2:6113567/140‑1 (MQ=255)
                      ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                                      >  1:1115413/1‑140 (MQ=255)
                      ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                                      >  2:3375369/1‑140 (MQ=255)
                      ccgTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAg                                                                                                                      >  2:5594415/1‑140 (MQ=255)
                          gaagaaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGctct                                                                                                                  <  1:6810550/140‑1 (MQ=255)
                              aaCTCGCGGAAGACCAGTTGTGGTACCGATCTCTTCCATCTCTTTGAAGACACGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAAGGCAGGTGCTAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                              <  2:5461518/140‑1 (MQ=255)
                              aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                              <  2:3003121/140‑1 (MQ=255)
                              aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                              <  1:2894114/140‑1 (MQ=255)
                              aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                              <  2:7487385/140‑1 (MQ=255)
                              aaCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                              <  2:3949789/140‑1 (MQ=255)
                               aCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCa                                                                                                             <  1:8224/140‑1 (MQ=255)
                               aCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCa                                                                                                             <  2:3591786/140‑1 (MQ=255)
                               aCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCa                                                                                                             <  2:6837981/140‑1 (MQ=255)
                               aCTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCa                                                                                                             <  1:3138776/140‑1 (MQ=255)
                                cgaGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCATTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                            <  2:4571520/138‑1 (MQ=255)
                                cTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                            <  2:1115413/140‑1 (MQ=255)
                                cTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                            <  2:7549034/140‑1 (MQ=255)
                                cTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCGTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCACTCTATGCCCGTGCGCTCAACAAGCTCTTGGcgc                                                                                                            >  2:2538732/1‑138 (MQ=255)
                                   gCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGTCCGTGCGCTCAACAAGCTCTTGGCActct                                                                                                         >  1:2071079/1‑140 (MQ=255)
                                   gCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCActct                                                                                                         >  1:3811760/1‑140 (MQ=255)
                                   gCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCActct                                                                                                         >  1:4670871/1‑140 (MQ=255)
                                     ggAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCa                                                                                                             >  2:2236766/1‑134 (MQ=255)
                                        aGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                            <  1:6095951/132‑1 (MQ=255)
                                        aGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAgg                                                                                                    >  2:1728037/1‑140 (MQ=255)
                                        aGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAgg                                                                                                    >  1:4967135/1‑140 (MQ=255)
                                         gACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAggg                                                                                                   <  1:1427909/140‑1 (MQ=255)
                                          aCCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                                  <  2:580715/140‑1 (MQ=255)
                                          aCCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                                  <  2:2416303/140‑1 (MQ=255)
                                          aCCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                                  <  1:430385/140‑1 (MQ=255)
                                            cAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGACAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCa                                                                                                <  2:3091704/140‑1 (MQ=255)
                                               ttGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCg                                                                                             <  1:2332354/140‑1 (MQ=255)
                                               ttGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCg                                                                                             <  1:4762407/140‑1 (MQ=255)
                                                    tttCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCTGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAATCTCTTGGCACTCTTTAGGGTCAGCGTAGAt                                                                                        >  1:2276764/1‑140 (MQ=255)
                                                         gATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGTCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGt                                                                                                  <  1:5836556/125‑1 (MQ=255)
                                                         gATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGc                                                                                   >  2:5971920/1‑140 (MQ=255)
                                                            ctctTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                              >  2:4849569/1‑110 (MQ=255)
                                                               ttCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAg                                                                             <  1:7649201/140‑1 (MQ=255)
                                                                         ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                            >  1:5208989/1‑99 (MQ=255)
                                                                         ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCAc                                                                                                            >  1:5340627/1‑99 (MQ=255)
                                                                         ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTc                                                                   <  1:6326993/140‑1 (MQ=255)
                                                                         ttGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTc                                                                   >  1:6149221/1‑140 (MQ=255)
                                                                         ttGAAGCCAAGGTTTGGCTCACCTGTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCACGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGGAAGCTTTAGGGTCAGCGTAGATAACGCCTTCAGCAATAACGTc                                                                   >  2:4691860/1‑140 (MQ=255)
                                                                                cAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCAtcttct                                                            <  1:4217375/140‑1 (MQ=255)
                                                                                cAAGGTTGGGCTCACCTGTGTAAGGGCCGTGAACAGACCCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCAtcttct                                                            <  2:5524990/140‑1 (MQ=255)
                                                                                 aaGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATcttctt                                                           >  2:2031922/1‑140 (MQ=255)
                                                                                         gCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTccgccg                                                   >  1:1828277/1‑140 (MQ=255)
                                                                                              ccTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGt                                              >  2:4399215/1‑140 (MQ=255)
                                                                                               cTTTGTAAGGGCCGTTAACAGAACCTAATGCTGGTGCAAGGCATTCGATGCCCGTGAGCTCAACAAGCTCTTGGCACGCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGtt                                             >  1:1611850/1‑140 (MQ=255)
                                                                                               cTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGtt                                             >  1:357243/1‑140 (MQ=255)
                                                                                               cTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGtt                                             >  1:569440/1‑140 (MQ=255)
                                                                                                 ttGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTcc                                           <  2:4937609/140‑1 (MQ=255)
                                                                                                     aaGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGc                                       <  2:1281114/140‑1 (MQ=255)
                                                                                                       gggCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTc                                     <  2:2218389/140‑1 (MQ=255)
                                                                                                        ggCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCa                                    >  1:4124577/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  2:790373/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  2:5059232/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  2:5330409/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  2:6316130/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  1:3699163/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  1:2461702/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  1:2723135/1‑140 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCg                                                                                             <  1:4903613/82‑1 (MQ=255)
                                                                                                         gCCGTGAACAGAACCTAATGCAGGTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAg                                   >  2:7250783/1‑140 (MQ=255)
                                                                                                          ccGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGc                                  <  2:6496133/140‑1 (MQ=255)
                                                                                                           cGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACCAGCTCTTGGCACTCTTTAGGGTCAGCGTAGAGTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCt                                 <  2:3864962/140‑1 (MQ=255)
                                                                                                           cGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCt                                 <  2:6195238/140‑1 (MQ=255)
                                                                                                             tGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                               <  1:7238856/140‑1 (MQ=255)
                                                                                                             tGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                               <  1:3319736/140‑1 (MQ=255)
                                                                                                             tGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                               <  1:6633762/140‑1 (MQ=255)
                                                                                                             tGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                               >  1:6228836/1‑140 (MQ=255)
                                                                                                             tGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                               >  1:5550285/1‑140 (MQ=255)
                                                                                                             tGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCGTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTc                               <  1:5054837/140‑1 (MQ=255)
                                                                                                                  aGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAg                          >  2:519877/1‑140 (MQ=255)
                                                                                                                   gAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTGTAGGGTCAGCGTAGATTACGCCTTCAGAAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGa                         >  1:7409700/1‑140 (MQ=255)
                                                                                                                    aaCCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGc                                                                                                              >  1:3985870/1‑54 (MQ=255)
                                                                                                                    aaCCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGAt                        >  2:73369/1‑140 (MQ=255)
                                                                                                                    aaCCTAATGCAGGTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGAt                        >  2:6690052/1‑140 (MQ=255)
                                                                                                                     aCCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATa                       <  2:3500405/140‑1 (MQ=255)
                                                                                                                     aCCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATa                       <  1:4370397/140‑1 (MQ=255)
                                                                                                                     aCCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATa                       <  2:3811760/140‑1 (MQ=255)
                                                                                                                         aaTGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTcc                   >  1:4601417/1‑140 (MQ=255)
                                                                                                                         aaTGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTcc                   >  2:425409/1‑140 (MQ=255)
                                                                                                                           tGCAGGTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGt                 >  1:1952665/1‑140 (MQ=255)
                                                                                                                               ggTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAg                          <  2:1549932/127‑1 (MQ=255)
                                                                                                                                gTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGTCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGATTCTACAGATACTCCGTGGaaa            >  1:1003665/1‑140 (MQ=255)
                                                                                                                                gTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGaaa            >  2:3699582/1‑140 (MQ=255)
                                                                                                                                gTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCAATTGGCACTCTTTAGGGTCACCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATAATCCGTGGaaa            >  1:6942081/1‑140 (MQ=255)
                                                                                                                                gTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGaaa            >  1:4405906/1‑140 (MQ=255)
                                                                                                                                gTGCAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGaaa            >  1:2667694/1‑140 (MQ=255)
                                                                                                                                   cAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGa                                                  <  2:1007445/99‑1 (MQ=255)
                                                                                                                                   cAAGGCACTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGAAATGa         <  2:1472341/140‑1 (MQ=255)
                                                                                                                                          gTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCt                                 <  1:2224962/109‑1 (MQ=255)
                                                                                                                                          gTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGAAATGAGCAAGCt  >  1:754663/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
AGCAGTCGGAATTCCAGTACCGCCGTGAAGAACTAGCGGAAGACCAGTTGTGTTTCCGATCTCTTCCATCTCTTTGAAGCCAAGGTTTGGCTCACCTTTGTAAGGGCCGTGAACAGAACCTAATGCAGGTGCAAGGCAGTCGATGCCCGTGCGCTCAACAAGCTCTTGGCACTCTTTAGGGTCAGCGTAGATTACGCCTTCAGCAATAACGTCATCTTCTTGTCCGCCGACTGTTCCAAGCTCAGCTTCTACAGATACTCCGTGGAAATGAGCAAGCT  >  NZ_CP034943/3635845‑3636122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: