BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,719,864	T→G	5.5%	S79R (AGC→CGC)	EO946_RS19145 ←	YwdI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,719,864	0	T	G	5.5%	1448.1 / 18.5	639	S79R (AGC→CGC)	EO946_RS19145	YwdI family protein
Reads supporting (aligned to +/- strand): ref base T (283/296); new base G (6/54); total (289/350)
Fisher's exact test for biased strand distribution p-value = 1.03e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 644 total aligned reads displayed.

AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001

     tGTCCATTGCAGCGCCCCAAGAACCAATTTCACATACCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCGTCTCTTTCTCCTGCTTGAGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  2:5625837/1‑140 (MQ=255)

     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  2:2570601/1‑140 (MQ=255)

     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTGTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  2:6629257/1‑140 (MQ=255)

     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTGCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  2:3120329/1‑140 (MQ=255)

     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCCTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  1:6857370/1‑140 (MQ=255)

     tGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCGCTTGCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCca                                                                                                                                      >  1:986425/1‑140 (MQ=255)

       tCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCcatc                                                                                                                                    >  1:5289364/1‑140 (MQ=255)

            tGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttt                                                                                                                               >  1:3026500/1‑140 (MQ=255)

                gcgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTcc                                                                                                                           >  1:2127247/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:4924504/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:6740187/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:727104/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  2:1438615/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  2:3389930/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:2331854/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCca                                                                                                                          >  1:1523426/1‑140 (MQ=255)

                 cgcACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCATGATTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTcaa                                                                                                                          >  2:6281700/1‑138 (MQ=255)

                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCGTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGAGTCCCATCATTTTTTCcata                                                                                                                        >  2:4509043/1‑139 (MQ=255)

                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        >  2:3733151/1‑140 (MQ=255)

                   cACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTGTCGATATTTGTTTAATACCGCGCTTCCCATCATTTTTTCcatc                                                                                                                        >  1:3169133/1‑140 (MQ=255)

                   cACCAAGAACCAATTAAACATAGCTCAAACTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCACTTGCTGATTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCTCGTCCCATCAATTTTTCaatc                                                                                                                        <  2:4819600/140‑1 (MQ=255)

                        aGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAg                                                                                                                   <  1:3314936/140‑1 (MQ=255)

                        aGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAg                                                                                                                   <  1:375842/140‑1 (MQ=255)

                           aCCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCGTGCTTGTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTg                                                                                                                >  1:5357966/1‑140 (MQ=255)

                             cAATTTCACATCGCTCCACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  1:1026271/140‑1 (MQ=255)

                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGt                                                                                                              <  2:6454090/140‑1 (MQ=255)

                             cAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTAGTTTAATCCCGCGCTTCCCAACATTTTTTCCATCATCAGCTGGt                                                                                                              <  1:1877694/140‑1 (MQ=255)

                                tttCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTGCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTg                                                                                                           >  2:2237761/1‑140 (MQ=255)

                                       tcgCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTGCAGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCCGCCGCTGGTCTGTCGATgg                                                                                                    <  2:1980951/138‑1 (MQ=255)

                                       tAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  2:4078174/140‑1 (MQ=255)

                                       tAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATgg                                                                                                    <  2:7731940/140‑1 (MQ=255)

                                        aGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGc                                                                                                                  >  1:5050801/1‑125 (MQ=255)

                                        aGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGc                                                                                                   >  1:3476612/1‑140 (MQ=255)

                                        aGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTACATCATCAGCTGGTCTGTCGATGGc                                                                                                   >  1:4649/1‑140 (MQ=255)

                                          cTCAACCTTTCTCATTAAAAATCAAATAAATACTCTCAGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCATCTGGTCTGTCGATGGCgg                                                                                                 >  1:3667496/1‑140 (MQ=255)

                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 <  1:7145257/140‑1 (MQ=255)

                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 >  2:1538320/1‑140 (MQ=255)

                                          cTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCgg                                                                                                 <  1:5775000/140‑1 (MQ=255)

                                             aaCCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATCTTTCTTTAATCCCGCGCTTCCCATCATATTTTCCAACATCAGCTGGTCTGTCGATGGCGGTGc                                                                                              >  2:4947835/1‑140 (MQ=255)

                                                  ttCTCATTAAAAATCAAATACAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAg                                                                                         <  2:7226814/140‑1 (MQ=255)

                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  2:5248076/1‑140 (MQ=255)

                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  1:6391066/1‑140 (MQ=255)

                                                    ctcATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCGCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGa                                                                                       >  1:5453548/1‑140 (MQ=255)

                                                      cATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACt                                                                                     <  1:2223521/140‑1 (MQ=255)

                                                       aTTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCAtttttt                                                                                                                             >  2:1748224/1‑99 (MQ=255)

                                                         tAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCgg                                                                                  >  2:388383/1‑140 (MQ=255)

                                                           aaaaTCAAATACAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCg                                                                                <  2:7573866/140‑1 (MQ=255)

                                                             aaTCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              <  1:2662895/140‑1 (MQ=255)

                                                             aaTCAAAGACAGACTCGCCGTGTCCGTCCTCGTCCTGCTTCTCTTTCTCCTGCTTTCGATAGTTGGTTACTCCCGCGCTTCCCATCATTTTTTCCATCATCCGCTGGTCGGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              <  2:6933290/140‑1 (MQ=255)

                                                                  aagcaaGACGCTCCGTGTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:4259268/136‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:3554947/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:4740261/140‑1 (MQ=255)

                                                                  aaTAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:2144986/140‑1 (MQ=255)

                                                                      aaGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  1:1153812/140‑1 (MQ=255)

                                                                             tcCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAAGCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTgctgct                                                                         <  2:5873561/129‑1 (MQ=255)

                                                                             tcCGTTTCCGGCCTCTTCCTGCGTGTCTTTCTCCTGCTTTCGATATTGGTTTAATCCCGCGGTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGAGGCAGGAGTCGGCGTGCTGCTCGGCTGTATGc                                                              >  1:2015676/1‑140 (MQ=255)

                                                                             tcCATTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGc                                                              >  1:3388551/1‑140 (MQ=255)

                                                                               cGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCgg                                                            >  1:2865698/1‑140 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  1:2966982/1‑140 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCt                                                    >  1:663655/1‑140 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTGCCATCCTTTTTTCCTTCATCAGGGGGTGTGTCGTGGGCGGTGCGGCAGGACTCGGGGTGAGGCGGGGCTGTATGCGGGGCGGGCt                                                    >  2:303721/1‑140 (MQ=255)

                                                                                       tCCTCTTCCTGCTTCTCTTTATCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATAATCAGCTCGTATGTCGATGGCGGTGAGGCAGGACTCGTCCTGCTGCTCGGCAGTATGCGGGGCGCGCt                                                    >  1:4291241/1‑140 (MQ=255)

                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  1:3538613/140‑1 (MQ=255)

                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  2:2865698/140‑1 (MQ=255)

                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  1:137212/140‑1 (MQ=255)

                                                                                         ctctTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTcg                                                  <  1:2520469/140‑1 (MQ=255)

                                                                                            ttCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCgg                                               >  2:1807854/1‑140 (MQ=255)

                                                                                               cTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCCTCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGtct                                            <  2:1111397/140‑1 (MQ=255)

                                                                                               cTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGtct                                            <  1:4487952/140‑1 (MQ=255)

                                                                                               cTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGtct                                            <  1:4799921/140‑1 (MQ=255)

                                                                                                  cTTCTCTTTCTCCTGCGTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCg                                         >  1:3869770/1‑140 (MQ=255)

                                                                                                   ttCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCgg                                        <  1:6545672/140‑1 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCCTCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  1:4069225/140‑1 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  1:5853408/140‑1 (MQ=255)

                                                                                                    tctcTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGc                                       <  1:6139956/140‑1 (MQ=255)

                                                                                                      tctTTCTCCTGCTTTCGATATTTGTTTAATCTCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              >  2:2768094/1‑99 (MQ=255)

                                                                                                      tctTTCTCCTGCTTTCGATATTTGTTTAATCTCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGtg                                                                              <  1:2768094/99‑1 (MQ=255)

                                                                                                       ctTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGa                                    <  2:3489782/140‑1 (MQ=255)

                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCCTCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCACGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  1:1764622/140‑1 (MQ=255)

                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             >  2:6727842/1‑140 (MQ=255)

                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  1:762040/140‑1 (MQ=255)

                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  2:5357966/140‑1 (MQ=255)

                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  2:5104495/140‑1 (MQ=255)

                                                                                                              cTGCTTTCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  2:5398101/140‑1 (MQ=255)

                                                                                                              cTGCTTTCGATATCTGTTTAATCCCGCGATTCCCATCATTTTTTCCATCATCAGCGGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCa                             <  2:5764373/140‑1 (MQ=255)

                                                                                                                    tCGATATTTGTTTAATCCCGCGCGTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGt                       <  2:7371242/140‑1 (MQ=255)

                                                                                                                          tttGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTcacaca                 <  2:2314665/140‑1 (MQ=255)

                                                                                                                               ttAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGaa            <  2:7476341/140‑1 (MQ=255)

                                                                                                                               tgaaTCCCGCGCGTCCCATCATTTTTTCCCTCAACAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGaa            <  1:6069877/138‑1 (MQ=255)

                                                                                                                                  aTCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     >  2:870349/1‑80 (MQ=255)

                                                                                                                                  aTCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGc                                                                     <  1:870349/80‑1 (MQ=255)

                                                                                                                                  aTCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACgg         >  2:324004/1‑140 (MQ=255)

                                                                                                                                  aTCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACgg         >  1:7473650/1‑140 (MQ=255)

                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  2:6121031/140‑1 (MQ=255)

                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:792445/140‑1 (MQ=255)

                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:6457451/140‑1 (MQ=255)

                                                                                                                                    cccGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  1:1589367/140‑1 (MQ=255)

                                                                                                                                    cccGCGCGTCCCATCATTTTTTCCATCAGCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGCCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGAc       <  2:7055790/140‑1 (MQ=255)

                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  2:1301649/1‑140 (MQ=255)

                                                                                                                                        cgcTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAAcc   >  1:5604498/1‑140 (MQ=255)

AACGCTGTCCATTGCAGCGCACCAAGAACCAATTTCACATAGCTCAACCTTTCTCATTAAAAATCAAATAAAGACTCTCCGTTTCCGTCCTCTTCCTGCTTCTCTTTCTCCTGCTTTCGATATTTGTTTAATCCCGCGCTTCCCATCATTTTTTCCATCATCAGCTGGTCTGTCGATGGCGGTGCGGCAGGACTCGGCGTGCTGCTCGGCTGTATGCGGGGCGCGCTCGCGGTCTCCGGCTGATCCAGCACGACGTCACACAAAGAACGGACAACCG  >  NZ_CP034943/3719725‑3720001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`