BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,893	T→G	7.4%	N59H (AAC→CAC)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,893	0	T	G	7.4%	1153.5 / 18.9	451	N59H (AAC→CAC)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base T (100/299); new base G (50/2); total (150/301)
Fisher's exact test for biased strand distribution p-value = 1.52e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 476 total aligned reads displayed.

TTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAA  >  NZ_CP034943/3746755‑3747031

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                                                          >  2:7326058/1‑140 (MQ=255)

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtttc                                                                                                                                          >  2:5783636/1‑140 (MQ=255)

 ttGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAAACCCGTCGtttg                                                                                                                                          >  2:6561889/1‑139 (MQ=255)

      gAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGtgtattttt                                                                                                                                     >  1:301750/1‑138 (MQ=255)

         aTTCATAAATGACGCCTTTCGTATATTTCGAAAAGTCGTCTTCTTTTTTATAGAGTATGTCGTATAGGCTGCATTCATTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTgga                                                                                                                                  >  2:1506812/1‑138 (MQ=255)

          ttCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCaa                                                                                                                                 <  1:1011499/140‑1 (MQ=255)

          ttCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCaa                                                                                                                                 <  2:1792955/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  2:3228384/140‑1 (MQ=255)

              tAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  2:366712/140‑1 (MQ=255)

               aaaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagca                                                                                                                            <  1:433173/140‑1 (MQ=255)

                aaggACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGACATTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:7216919/137‑1 (MQ=255)

                aaTGACGCCTTTCTGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:3901141/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:5067334/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:5042733/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:4810294/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:4915451/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:6805197/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:5659818/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:576498/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:65528/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:6267799/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:1040765/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:169040/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:1877300/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:2323199/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:7630705/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACCTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  1:1351790/140‑1 (MQ=255)

                aaTGACGCCTTTCGGATAGGTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATCCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagcag                                                                                                                           <  2:6895435/140‑1 (MQ=255)

                    aCGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAAAAGCAGCcta                                                                                                                       >  2:320360/1‑140 (MQ=255)

                      gCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                                                                     <  1:2868496/140‑1 (MQ=255)

                      gCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                                                                     <  1:3636841/140‑1 (MQ=255)

                      gCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                                                                     <  2:5634186/140‑1 (MQ=255)

                       ccTTTCGGATAGTTCGCCCAGTCGTCGTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCATCaaattt                                                                                                                    >  2:5347582/1‑138 (MQ=255)

                        cattCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                                                   <  1:4711054/138‑1 (MQ=255)

                        cTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTa                                                                                                                   <  1:832814/140‑1 (MQ=255)

                           tCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAcagc                                                                                                                             <  1:148851/127‑1 (MQ=255)

                                   ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAAAGCAGCATATTTAACCAATGTCAg                                                                                                        >  1:5478948/1‑140 (MQ=255)

                                   ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAACAGCAGCATATTTAACCAttttaag                                                                                                        >  2:4665721/1‑135 (MQ=255)

                                     cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcg                                                                                                      <  2:7482390/140‑1 (MQ=255)

                                       cccTGTAGTCTTCTTTTTGATAGCGGATGTCGTCTATGCTGCATTACTTTATCCCTACGTCTTTACAGTTGTCTGAATACATCCGGACAACCCCGTAGTTTCTTTCTGCAACAGCAGCATATGTAACGATTGTCAgcgcg                                                                                                    <  2:4405054/140‑1 (MQ=255)

                                       cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAgcgcg                                                                                                    <  2:1357306/140‑1 (MQ=255)

                                            tcgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCCACAGCCGCATAGTTACCCAATGTCAGCGCGCGCgg                                                                                               >  2:7192117/1‑140 (MQ=255)

                                            tcgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCAGCATATGTACCCCTTGTCAGCGCGCACgg                                                                                               >  2:7081532/1‑140 (MQ=255)

                                            tcgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGGTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCAGTGTCAGCGCGCACgg                                                                                               <  1:2123311/140‑1 (MQ=255)

                                               tcttctTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGaaa                                                                                            <  1:155086/140‑1 (MQ=255)

                                                 ttcttTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTTCAACACCAGCATATGTAACCAATGTCAGCGCGCACGGAAaca                                                                                          >  2:7607134/1‑140 (MQ=255)

                                                      tttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCACCAAATTTACCCATTGTCAGCGCGCACGGAAACACATGc                                                                                     >  2:4875399/1‑140 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTCCCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:316827/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:7685555/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:2656374/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:3292652/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:3485327/140‑1 (MQ=255)

                                                       ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:6252527/140‑1 (MQ=255)

                                                       ttGAGAGCGGCTGTCGTCTAGGCGGCAGTCCTGGATCCCACCGTCTTTAAAGGTGTCTGAAGACATCCGGACAACCCCGGCGTTTCTTTCAGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                    <  1:5920090/140‑1 (MQ=255)

                                                         gATATCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAACAGCAGCATGTGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  2:416384/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTCGGCTGCATTCCTTGATCCCTACGTCTTTACAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:5303318/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACCGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  2:2965867/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:7049111/140‑1 (MQ=255)

                                                         gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                  <  1:4121145/140‑1 (MQ=255)

                                                         gATAGCGAATGTCGTCAAGGCTGCATTCCTTGATGCCTGCGTTTCTAAAGTTATCTGAATACATCCGGACAAGCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACATGCccg                                                                                  <  1:6052865/140‑1 (MQ=255)

                                                               ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGCATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                                            <  2:1979844/140‑1 (MQ=255)

                                                               ggATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTta                                                                            <  2:6724047/140‑1 (MQ=255)

                                                                gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                           <  2:350838/140‑1 (MQ=255)

                                                                    tcgtcTAGGCTGGATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                       <  1:7189838/140‑1 (MQ=255)

                                                                     cgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCt                                                                      <  1:3723868/140‑1 (MQ=255)

                                                                           ggCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTCTTCCACAGCAGCAAGAGTACACATTGTCAGCGCGCCAGGCAACAAATGACCGCCCTTATAAAGTGTAttt                                                                >  1:4684419/1‑138 (MQ=255)

                                                                              tGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTGTCTGCCAAAGCCCCAAATGTACCCATTGTCACCGCGCACGCCAACACATTCCCGCCGGTATAATCTGGTTGTtct                                                             >  2:779595/1‑140 (MQ=255)

                                                                              tGCATTCCTTGATCCCTACGTCTTTAAAGTTCTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCACCAAAATTCACCATTGTCAACCCGCACGGTAACACAACCCCCCCGTTATAATGGGTAAGtttg                                                             >  2:6816558/1‑138 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTATGCAACAGCAGCATATGTACCCATTGTCAGCGCGCAAGGAAAAACATGCCCGCCGGAATAATCAGTATTTTATcc                                                           >  2:3428504/1‑140 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCAAATTTAACCAATGTAAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGGAttgtatcc                                                           >  2:3194292/1‑133 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATTCAACACCAGCAAATGTAACCAAGTTAAGCGCGCACGGAAACACAATCCCGCCGGTATAAAAAGTATGTTCGcc                                                           >  2:6077092/1‑140 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTATGCAAAAGCACCAAATGTACCCCATGTCAGCGCCCACCGAAACAAAAGGCCCCCCGTAAAATAtgtttgttgtac                                                           >  1:1983620/1‑136 (MQ=255)

                                                                                cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGGAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAAACATGCCCGCCGTTATAATCTGTATGTTATcc                                                           >  2:2175544/1‑140 (MQ=255)

                                                                                   tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                        <  1:4783790/140‑1 (MQ=255)

                                                                                          tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                 <  1:1237919/140‑1 (MQ=255)

                                                                                          tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                 <  2:1189231/140‑1 (MQ=255)

                                                                                           cccTACCTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCa                                                <  2:903727/140‑1 (MQ=255)

                                                                                             cTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCAGTCGTGTATTTCTGGAACAGCAGAAAATGTACACATTGTAAACGCGCAAGGAAACAAAAGCCACCCGTTATAAAATGTATGTTATCCAATAAGAtataag                                              >  2:6366938/1‑135 (MQ=255)

                                                                                               aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTGTGCAACACCACCATATTTAGCCAGTGTCAGCCCCCACCGGAAAACAAGACCGCCGGTATAAAATTTATGTTGTACCAACAGATTAActat                                            >  1:4611198/1‑138 (MQ=255)

                                                                                                    tttAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATTTACCCATTGTCAGCGCGCACGGAAACACATGCCCGGCGTTATAATCTGTATGTTCTCCAATAAGATTCACTCTTCCAg                                       >  2:7586559/1‑140 (MQ=255)

                                                                                                    tttAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTCTGCAAAACCAGCATATGGACCCATTGTAAGCGCGCACGGAAACAAATGCCCCCCGTTATAATCTGTAAGTTCTCCAATCAGATTCACTCTTCCAg                                       >  2:3361179/1‑140 (MQ=255)

                                                                                                      tAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATTCAACAGCAGCATATTTAACCATTGTAAGCCCGCACGGAAACAAATTCCCCCCGTTATAAACGGTATGTTATACAATAACAATCAAACTTCCAGGt                                     >  2:1559821/1‑140 (MQ=255)

                                                                                                         aGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCg                                  <  2:5481204/140‑1 (MQ=255)

                                                                                                         aGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTCCAACAGCAGCATATGTACCCCTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCg                                  <  1:4069204/140‑1 (MQ=255)

                                                                                                             gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTGTGCAAAAGCAGCATATGTACCCATTGTCAGCGCGCCCCGAAACACAAGCCCGCCGGTATAATCTGTATGTTCTCCCAACATATTCACGCTTCCAGGTGCGaaaa                              >  2:2780405/1‑140 (MQ=255)

                                                                                                             gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCAACAAATGTACCCATTGTCACCGCGCAACGAAAAAAAAGACCGCCCTTATAATATGTAAGTTATCCAATCAGATTGACTATTCAAGGTCCGaaaa                              >  2:4290223/1‑140 (MQ=255)

                                                                                                              tCTGAATACATCCGGACAACCCCGTCGTTTGTTTATGCAACAGCACCAAAAGTAACCAATGTCACCGCGCCCCGAAAAAAAAGCCCCCCCATATAAAATGTATGTTCTACAAGCACATTCAAAATTCCAGGTGCGaaaaa                             >  2:632002/1‑140 (MQ=255)

                                                                                                              tCTGAATACATCCGGACAACCCCGTCGTTTCGTTATTCCACAGCAGCCTATGTACCCAATGTCAGCGCGCACGGAAACACAAGCCCGCCCTTATAAACTGGATGTTGGCCAATCAGATTCACAGATCCAGGTGCGcaaaa                             >  2:5155717/1‑140 (MQ=255)

                                                                                                               cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaa                            <  2:5973602/140‑1 (MQ=255)

                                                                                                               cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaa                            <  2:6969970/140‑1 (MQ=255)

                                                                                                                     aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGt                      <  2:2643555/140‑1 (MQ=255)

                                                                                                                     aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGt                      <  2:6383528/140‑1 (MQ=255)

                                                                                                                     aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGt                      <  2:4498058/140‑1 (MQ=255)

                                                                                                                        tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCCTTTTCAGCGCGCACGGAAACACATGCCCGCCGTTATAAACTGTATGTTCTCCAATCAGATTCACACTTCCAGGTGCGAAAAAAAAACGGAAc                   >  2:3705003/1‑140 (MQ=255)

                                                                                                                        tCCGGACAACCCCGTCGTGTGTTTCTGCAACAGCAGCATATGTACCCATTTTCCGCGCGCACCGAAAAACATGCCCGCCCTTATAAACTGTATGTGATCCAATAACATTCACTCTTACACGTTCGAAAAAAAAACCTAAc                   >  2:6872337/1‑140 (MQ=255)

                                                                                                                         ccGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCAAATGTACCCATTGTCAGCGCCCACGGAAACACATGCCCGCCGTTATAAAATGTATGTTATACAATCCGATTAACTAGTCCAGGTGCGAAAAAAAACCCGAAcc                  >  2:2496230/1‑140 (MQ=255)

                                                                                                                            gACAACCCCGTCGTGTGTTTATGCAACAGCCGCCTATGTACCCAGTGTCAGCGCGCCCGGAAACACATGCCCGCCGGTATAATCTGGATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGGAAccccg               >  2:3120355/1‑139 (MQ=255)

                                                                                                                                    cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct       <  2:1864040/140‑1 (MQ=255)

                                                                                                                                    cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct       <  1:6369505/140‑1 (MQ=255)

                                                                                                                                    cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct       <  1:1102006/140‑1 (MQ=255)

TTTGCTGAAATTCATAAATGACGCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAA  >  NZ_CP034943/3746755‑3747031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`