BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,962	C→A	6.4%	D36Y (GAT→TAT)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,962	0	C	A	6.4%	1235.2 / 9.3	477	D36Y (GAT→TAT)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base C (229/185); new base A (52/4); total (288/189)
Fisher's exact test for biased strand distribution p-value = 8.38e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 489 total aligned reads displayed.

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTATT  >  NZ_CP034943/3746823‑3747101

          tGCATTCGTTGATCCCTACGGCTTTAAAGTTGTCTGAATACATCCGGACATCCCCGTCGTTTCTTTCTGCAAAAGCAGCATATGT‑ACCCAATGGCAGGGCGCACGGAAACACCTGCCCGCCGTGATAATAGGTATGTtct                                                                                                                                   >  2:1568573/1‑140 (MQ=255)

          tGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTGTGCAAAAGCAGCATATTT‑ACACAATTTCAGCGCGCACGGAAAAAAATGCCCCCCCGTATAATCTGTATGTtct                                                                                                                                   >  2:7159591/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTGTTCAACAGCAGCATATTT‑ACCCATTGTCAGCCCGCACGGAAACACAAGCCCCCCGGTATAATATGTATGttatac                                                                                                                                 >  1:3225923/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTTCAACAGCAGCAAATGT‑AACCATTTTCAGCGCGCACGGAAACACATTCCCGCCGATATAATATGTATTTTCAcc                                                                                                                                 >  2:1891559/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCACCAAATTT‑ACCCATTGTCAGCGCGCCCCGAAACACATGCCCGCCGTTATAAAAGGTATGttgtgc                                                                                                                                 >  2:223793/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTATGCAACAGCAGCAAATGT‑ACCCCTTTTCAGCGCGCACGGAAACACAAGCCCCCCGTTATAATCTGTATGTTGTcc                                                                                                                                 >  1:5814646/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTTCAACAGCACCCAAAGTGACCCATT‑TCAGCCCCCACCGAAACACAAGCCCCCCCGTATAAAATGTATGTTATcc                                                                                                                                 >  1:7117575/1‑140 (MQ=255)

               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                                                              <  1:4783790/140‑1 (MQ=255)

                 cTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAAAAGCAGCCTATGT‑ACCCAATGTCAGCGCGCCCCGAAAAAAAAGCCCGCCCGTATAATATGGATGTTATGCAATCa                                                                                                                            >  1:6225170/1‑140 (MQ=255)

                   tGATACCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGa                                                                                                                          <  2:1834510/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                                                                                       <  2:7079196/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTGTGCAAAACCAGCATATGT‑ACCCAATGTCAGCGCGCACGGAAACACAAGCCCGCCCTTATAATATGTATTTTGTACAAACAAAtta                                                                                                                       >  1:1282544/1‑139 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCGTTATGCAACACCAGCATATGT‑TCCCAGTGGGAGCGCGCACGGAAACAAATGCCCGCCGGTATAATCAGTATTTTGGACAATCAGATTc                                                                                                                       >  1:3900013/1‑140 (MQ=255)

                                  tAATGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGAATATGG‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCACTCAGATTCACTCTTACAGGt                                                                                                           >  2:7595277/1‑140 (MQ=255)

                                  tAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGT‑ACCCAATGTCAGCGCGCACGGAAACACAAGCCCGCCGTTATAATCTGTATTTTGTCCAATCAGAATCACACTTACACGt                                                                                                           >  2:6333349/1‑140 (MQ=255)

                                     aGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTCCAACAGCAGCATATGT‑ACCCCTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCg                                                                                                        <  1:4069204/140‑1 (MQ=255)

                                         gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTGTGCAAAAGCAGCATATGT‑ACCCATTGTCAGCGCGCCCCGAAACACAAGCCCGCCGGTATAATCTGTATGTTCTCCCAACATATTCACGCTTCCAGGTGCGaaaa                                                                                                    >  2:2780405/1‑140 (MQ=255)

                                         gTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACAGCAGCATATGT‑AACCATTGTCAGCGCGCACGGCAACACATGCCCGCCGTTATAAACTGTATGTTGTGCAATCATATTAACTATTACAGGTGCGcaaa                                                                                                    >  2:6269057/1‑140 (MQ=255)

                                           cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaa                                                                                                  <  2:6969970/140‑1 (MQ=255)

                                           cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaa                                                                                                  <  2:5973602/140‑1 (MQ=255)

                                           cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGG‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaa                                                                                                  <  2:6894003/140‑1 (MQ=255)

                                               aTACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCAATTTCAGCGCGCACGGAAACACATGCCCGCCGTTATAAACTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGaaaaaaaaac                                                                                              >  2:7566378/1‑138 (MQ=255)

                                                 aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGGTTTTGT‑ACCCATTGTCATCGCGCACGGAAACACATGCCCGCCGTTATATTCTGTCTGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGt                                                                                            <  2:3205064/140‑1 (MQ=255)

                                                 aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGt                                                                                            <  2:2643555/140‑1 (MQ=255)

                                                    tCCGGACAACCCCGTCGTTTGTTTCGTCAAAAGCCGCATATTG‑ACCCATTGTCAGCGCGCACGGCAAAACATTCCCGCCGTTATAAAATGTATTTTCTCCCAACACAATCACTCTTAAACGTGCGAAAAAAAAACGTAAc                                                                                         >  2:509371/1‑140 (MQ=255)

                                                    tCCGGACAACCCCGTCGTGTGTTTCTGCAACAGCAGCATATGT‑ACCCATTTTCCGCGCGCACCGAAAAACATGCCCGCCCTTATAAACTGTATGTGATCCAATAACATTCACTCTTACACGTTCGAAAAAAAAACCTAAc                                                                                         >  2:6872337/1‑140 (MQ=255)

                                                     ccGGACAACCCCGTCGTTTCTTTATTCAACAACAGCAAAATT‑AACCAGTGTCAAGCCCCAACGAAACACAACCCCCCCGTTATAATAAGTATTTTATAGCACCAGATTGAATAATGAACGTGCGAAAAAAAACCCTaaac                                                                                        >  2:4557098/1‑138 (MQ=255)

                                                      cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAccc                                                                                       <  2:1315878/140‑1 (MQ=255)

                                                        gACAACCCCGTCGTGTAGTTATGCAACAGCAGCCAAAGT‑ACCCAAAGGCAGCGCGCACGGAAACACCTGCCCGCCGGTATAATGTGTATGTTCTCCCAGCAGCTTCACTCTTCCAGGTGGGCAAAAAAACCGTAAccccg                                                                                     >  2:5234422/1‑139 (MQ=255)

                                                             ccccGTCGTTTCTTTCTGCAACACCCCCCTATGT‑AACCATTGTCAGCGCGCACGGAAACACAGGCCCGCCGGTATAAACTGGTTGTTCTCCAATCAGATTAACTCTTCCCGGGGCGAAAAAAAACCCTAACCCCTCtttt                                                                                >  2:3676516/1‑140 (MQ=255)

                                                               ccGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtc                                                                              <  1:7682525/140‑1 (MQ=255)

                                                                cgtcgtTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct                                                                             <  2:6097170/140‑1 (MQ=255)

                                                                     tttctttcTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGaa                                                                        <  1:6287809/140‑1 (MQ=255)

                                                                           tctGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACt                                                                  <  1:6824596/140‑1 (MQ=255)

                                                                               cAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCa                                                              >  2:3496450/1‑140 (MQ=255)

                                                                                aaCAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCaa                                                             <  1:2179148/140‑1 (MQ=255)

                                                                                   agcagcATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                          <  2:2289845/140‑1 (MQ=255)

                                                                                    gcagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAatat                                                         <  1:5387049/140‑1 (MQ=255)

                                                                                    gcagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAatat                                                         <  1:1221384/140‑1 (MQ=255)

                                                                                    gcagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAatat                                                         <  2:4103972/140‑1 (MQ=255)

                                                                                     gacagtatGT‑ACCATTTTTAAGGGCGCACGGAAACACATGCCCGCCTTTATAAAATGTATGTTCTCCAAGCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:5715321/135‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:6561889/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:6541593/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:4588753/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTGGCAAATATc                                                        >  2:5367382/1‑140 (MQ=255)

                                                                                     cagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCACGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:2446697/140‑1 (MQ=255)

                                                                                           atGT‑ACCCATTGTCAGCGAGCACGGAAACAGATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAATCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCttt                                                  <  1:4380766/140‑1 (MQ=255)

                                                                                             gT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTACCCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                >  1:6327348/1‑140 (MQ=255)

                                                                                             gT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                >  1:7236966/1‑140 (MQ=255)

                                                                                              gttccaaTTTTCAGCGCGCTCGGAAACACATGCACGCCGTTATAATCTGTATGTTCTCAAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAACCCTCTTGTTCTCCGTATACACTTGCAAATATCCCCTTTAg                                                <  2:4392018/134‑1 (MQ=255)

                                                                                              ggaCCCATTGTCAGGTCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCATATTCACTCTTCCAGGTGAGAACAAAAACCGTAACCCCTATTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  2:37386/138‑1 (MQ=255)

                                                                                              ggaCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  2:3965181/138‑1 (MQ=255)

                                                                                              ggaCCCAGTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAGGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  1:6052533/138‑1 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCTCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                              <  2:2025255/140‑1 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                              >  1:6421954/1‑140 (MQ=255)

                                                                                                aCCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                              <  2:6407447/140‑1 (MQ=255)

                                                                                                  ccATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGctct                                            <  1:1972564/140‑1 (MQ=255)

                                                                                                   cATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTc                                          >  2:744809/1‑140 (MQ=255)

                                                                                                    aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtta                                          >  2:6221414/1‑139 (MQ=255)

                                                                                                    aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTc                                          >  2:5774101/1‑140 (MQ=255)

                                                                                                    aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTc                                          >  1:349416/1‑140 (MQ=255)

                                                                                                    aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                         >  1:1682476/1‑140 (MQ=255)

                                                                                                     ttGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                                <  1:5936000/133‑1 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGACACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATAAACTTGCAAATATCCCCTTTATCTCTTccc                                        >  2:7222272/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                                                                        <  2:2972320/44‑1 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCa                                                                                                                      >  2:541022/1‑62 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTCACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  2:141683/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTCACCCCTCTTGTTCTCCGAATACACTTGCACATATCCCCTTTAGCTCTTccc                                        >  1:5207855/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  2:7037608/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  2:878117/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:3418078/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:5041409/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:5588499/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  2:1011869/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGGAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  1:2566808/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGCACGGAAACACATGAAATCCTTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  2:668864/1‑140 (MQ=255)

                                                                                                      tGTCAGCGCGAACGGAAACACATGCACTCCGTTATAATCTGTATGTTCTCCAATCAGAATCAATCTACAAGGTCCGAAAAAAAACCTTAACACATCTTAATATACGAAAAAAATTCAAAATATCCCCATTAGCTCTTccc                                        >  2:4824759/1‑140 (MQ=255)

                                                                                                          aGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        >  2:1227124/1‑120 (MQ=255)

                                                                                                          aGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAt                                    >  2:5601895/1‑140 (MQ=255)

                                                                                                          aGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAt                                    <  1:1002222/140‑1 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                  >  2:5690758/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                  >  2:7769521/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                  >  2:4752042/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                  >  2:4993681/1‑140 (MQ=255)

                                                                                                            cgcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCACTCTTTTTCTCCGAATAAACTTGCAAATATCCCCTTTAGCTCTTCACGCTtgt                                  >  2:1187412/1‑140 (MQ=255)

                                                                                                             gcgcACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgtg                                 >  1:2969569/1‑140 (MQ=255)

                                                                                                                cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTACCCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                              >  2:1695111/1‑140 (MQ=255)

                                                                                                                cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAGCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                              >  2:3369295/1‑140 (MQ=255)

                                                                                                                        cacaTGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTGCTCCGAATACACTTGCAAAGATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCttt                      <  2:2388790/140‑1 (MQ=255)

                                                                                                                          caTGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCa                    <  1:1108413/140‑1 (MQ=255)

                                                                                                                           aTGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCAt                   >  1:4705084/1‑140 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTCACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCGTACATTg                 >  1:2648414/1‑140 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCaa                                                             >  2:304641/1‑96 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTg                 >  1:7556861/1‑140 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTg                 >  2:4304272/1‑140 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTg                 >  1:3661684/1‑140 (MQ=255)

                                                                                                                                 gccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGtttt             >  1:4889015/1‑140 (MQ=255)

                                                                                                                                           tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  1:801912/140‑1 (MQ=255)

                                                                                                                                           tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  1:1500238/140‑1 (MQ=255)

                                                                                                                                            cTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttatt  <  1:2406256/140‑1 (MQ=255)

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTATT  >  NZ_CP034943/3746823‑3747101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`