Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,769,576 T→G 6.5% I281S (ATT→AGT)  sacX → SacY negative regulator SacX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,769,5760TG 6.5% 1491.0 / 19.5 603I281S (ATT→AGT) sacXSacY negative regulator SacX
Reads supporting (aligned to +/- strand):  ref base T (175/361);  new base G (63/3);  total (239/364)
Fisher's exact test for biased strand distribution p-value = 2.01e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 624 total aligned reads displayed.

AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGC  >  NZ_CP034943/3769437‑3769712
                                                                                                                                           |                                                                                                                                        
 aGCTATTAAAATCAATTGTGCCGAAGTAGCTTGATGTCGTCATCATTCCTTTTATTCCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATAGGCCAAATTATGACACAGTCGAtt                                                                                                                                         <  2:3238658/140‑1 (MQ=255)
 aGCTATGAAAATCAAGTGTGCCGAAGTCGCTTGCTGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAtt                                                                                                                                         <  2:6907092/140‑1 (MQ=255)
   cTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGt                                                                                                                                       <  2:4984447/140‑1 (MQ=255)
     aTTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCt                                                                                                                                     <  2:5071443/140‑1 (MQ=255)
      ttAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGTATCACGGCGAATATATCATCGGTAAAATTATGACACAGTCGATTGTCta                                                                                                                                    <  2:1808876/140‑1 (MQ=255)
      ttAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCta                                                                                                                                    <  1:1480684/140‑1 (MQ=255)
      ttAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCta                                                                                                                                    <  2:4505717/140‑1 (MQ=255)
      ttAAAATCAAGTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCta                                                                                                                                    <  1:7227464/140‑1 (MQ=255)
          aaTCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATAtt                                                                                                                                >  2:7370041/1‑140 (MQ=255)
          aaTCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTGTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGCTTGTCTAAAtt                                                                                                                                >  2:5416550/1‑140 (MQ=255)
           aTCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATAttt                                                                                                                               <  2:6833672/140‑1 (MQ=255)
           aTCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATAttt                                                                                                                               <  2:2190293/140‑1 (MQ=255)
              aaTTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATg                                                                                                                            >  2:356604/1‑140 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTGTAAATTtattat                                                                                                                          >  2:3591723/1‑140 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                          <  1:6879042/140‑1 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                          <  1:4266830/140‑1 (MQ=255)
                ttGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGAt                                                                                                                          <  2:6114726/140‑1 (MQ=255)
                  gtgCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCa                                                                                                                        <  1:5291263/140‑1 (MQ=255)
                   tgCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCAc                                                                                                                       >  1:7701712/1‑140 (MQ=255)
                         aGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGc                                                                                                                 <  2:6464124/140‑1 (MQ=255)
                              cTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgt                                                                                                            <  2:301586/140‑1 (MQ=255)
                              cTTGATGTCGTCATCAGTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCAttgt                                                                                                            <  2:3386052/140‑1 (MQ=255)
                                   tGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCg                                                                                                       <  2:1438485/140‑1 (MQ=255)
                                      cgtcATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAg                                                                                                    <  2:7263592/140‑1 (MQ=255)
                                      cgtcATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAg                                                                                                    <  1:579838/140‑1 (MQ=255)
                                       gtcATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCACGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATTATCACGCCGGCATTGTTTCCGGAGc                                                                                                   >  2:4874408/1‑140 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  1:6184500/140‑1 (MQ=255)
                                        tcatcaTTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  1:3569312/140‑1 (MQ=255)
                                        tcatcaGTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  1:6714366/140‑1 (MQ=255)
                                             atTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATGGTGTATATTTATGATCACGCCGGCATTGTTGGCGGAGCCCGGtt                                                                                             >  1:3792329/1‑140 (MQ=255)
                                               tCCTTTTAGACCGGCCATGGTGACAGGGTGCCTCGCGCTGATGGTGATGACTCCCGCCGCATCTATCATCGGCCAAATTAGGACACAGTCGATTGTCTATAATTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcg                                                                                           <  2:5558491/140‑1 (MQ=255)
                                                 cTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                         <  2:6057593/140‑1 (MQ=255)
                                                 cTTTTATTACGGTCAGGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggc                                                                                         <  2:5940976/140‑1 (MQ=255)
                                                  ttttATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTcggcg                                                                                        <  1:6054745/140‑1 (MQ=255)
                                                   tttATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTATATATTTATGATCAACCCGGCATTGTTGCCGGAGCCATGTTaggcgg                                                                                       >  1:697083/1‑140 (MQ=255)
                                                   tttATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATCTTTATGATCACGCCGGCATTGTTTCCGGATCCCTGTTcggcgg                                                                                       >  1:4016709/1‑140 (MQ=255)
                                                    ttattaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCTCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                      <  1:308929/140‑1 (MQ=255)
                                                    ttattaCGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTATATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCggcggc                                                                                      <  2:2320114/140‑1 (MQ=255)
                                                       ttaCGGTCAGGGTGACAGGGTGCCTCGCGCTGAGTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATc                                                                                   <  2:6080914/140‑1 (MQ=255)
                                                          cGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTGTATATTTATGATCACGCCGGCATTGTTTCCGGAGCCCGGTTCGGCGGCATCTAc                                                                                >  2:786902/1‑140 (MQ=255)
                                                           ggTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACt                                                                               >  2:2891333/1‑140 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGACTCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                              <  1:21230/140‑1 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                              >  1:4149526/1‑140 (MQ=255)
                                                            gTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTc                                                                              <  1:1297252/140‑1 (MQ=255)
                                                                tGGTGACAGGGTGCCTCGCGCTGAGTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACACTCGGTGGGGGATATGTATGATCACGCCCGCAGTGTTGGCGGGGCCCAGTGGCGCGGCATATACTccacc                                                                          >  1:7516466/1‑140 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTGTATATTTATGATCACGCCGGCAGTGTTGCCGGAGCCCTGTTCGGCGGCATCTTCTccccca                                                                         >  1:5180582/1‑140 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGCGCCCTGTTCGGCGGCATCTACTccaccc                                                                         >  2:2544364/1‑139 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCCTCTACTccacca                                                                         >  1:2032167/1‑140 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  1:4755522/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  2:4664935/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  2:4066280/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  1:4610260/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         >  2:2084069/1‑140 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  2:6644980/140‑1 (MQ=255)
                                                                 ggTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTccacca                                                                         <  1:7053972/140‑1 (MQ=255)
                                                                      cAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGtt                                                                    <  1:2940046/140‑1 (MQ=255)
                                                                      cAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGtt                                                                    <  2:1319011/140‑1 (MQ=255)
                                                                       aGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGGCTATATTTATGATCACGCCGGCATTGTTGCCCGAGCCCGGTTCGGCGGCCTCTACGCCACCATCGGTc                                                                   >  2:2772527/1‑140 (MQ=255)
                                                                          gTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTAt                                                                <  1:657963/140‑1 (MQ=255)
                                                                          gTGCCTCGCGCTGAGTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTAt                                                                <  1:6410844/140‑1 (MQ=255)
                                                                           tGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATc                                                               <  2:2732163/140‑1 (MQ=255)
                                                                             ccTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTGTATATTTATGATCACGCCGGCATTGTTGCCCGAGCCCGCTTCGGCGGCATCTACTCCACCATCGGTATATACg                                                             >  1:2612289/1‑140 (MQ=255)
                                                                             ccTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCGATATTTAAGAACACGCCCGCATTGTTTCCCGAGCCCAGTTCGGCGGCATCAACTCCACCAACGTTCAAACCg                                                             >  1:965974/1‑140 (MQ=255)
                                                                               tCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATTATCACGCCGGGATTGTTTCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTGTATGCGGc                                                           >  2:5223545/1‑140 (MQ=255)
                                                                               tCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGc                                                           <  1:2708820/140‑1 (MQ=255)
                                                                                cgcgCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                          <  2:3794647/140‑1 (MQ=255)
                                                                                cgcgCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                          <  1:1989808/140‑1 (MQ=255)
                                                                                cgcgCTGATTGGTCTGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCt                                                          <  2:4385381/140‑1 (MQ=255)
                                                                                 gcgcTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATGGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCAACGTTCTATCCGGCtt                                                         >  2:6208951/1‑140 (MQ=255)
                                                                                 gcgcTGATTGTGATGAATCCCGCCGCAGCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTTTGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATGCGGCtt                                                         >  2:7083741/1‑140 (MQ=255)
                                                                                       cttGTGATGCATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCGACTCCACCATCGTTCTATCCGGCTTGcatca                                                   <  1:5693302/139‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                   <  2:7701712/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatca                                                   <  1:1047073/140‑1 (MQ=255)
                                                                                       aTTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTGTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTATATCCGGCTTGcatca                                                   >  1:2299503/1‑140 (MQ=255)
                                                                                        ttGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatcat                                                  <  1:3912225/140‑1 (MQ=255)
                                                                                        gtgtgATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGGATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGcatcat                                                  <  1:5523714/139‑1 (MQ=255)
                                                                                         tgtgATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGGCTATATTTATGATCACGCCGGCCTTGTTGGCGGAGCCCGGTTAGGCGGCATGTACTCCACCATCGTTCTATGCGGCTTGcctcaaa                                                 >  2:5750976/1‑138 (MQ=255)
                                                                                             atgaATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCtt                                             <  1:2837494/140‑1 (MQ=255)
                                                                                             atgaATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGTCTATATTTATTATCACGCCGGCCTTGTTGACGGACCCCTTTTCGGCGGCAACTACTCCACCATCGTTCAATACCGCTTGCATCATAGCtt                                             >  2:1921353/1‑140 (MQ=255)
                                                                                             atgaATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGAGTGGGGATATTTATGATCACGCCGGCCTTGTTTCCGGAGCCCTGTTCGGCGGGATCTACTCCACCATCGGTGTATGCGGCTTTCATCATAGCtt                                             >  2:4747177/1‑140 (MQ=255)
                                                                                                  tCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGcc                                                                                                  <  2:391282/82‑1 (MQ=255)
                                                                                                     cgccgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGa                                     <  1:321987/140‑1 (MQ=255)
                                                                                                      gccgcATCTATCATCGGCCAAATTCTGTCACCGTCGCTTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTGCGGCGGCCTCTACTCCACCCTCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGAt                                    <  2:7552096/140‑1 (MQ=255)
                                                                                                      gccgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGAt                                    <  2:4231465/140‑1 (MQ=255)
                                                                                                      gccgcATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGAt                                    <  2:1164871/140‑1 (MQ=255)
                                                                                                             cTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCa                             <  2:4918392/140‑1 (MQ=255)
                                                                                                              tATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCaa                            <  2:7515838/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        <  1:502664/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        <  1:7415378/140‑1 (MQ=255)
                                                                                                                  atcGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCCGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgct                        <  2:1537260/140‑1 (MQ=255)
                                                                                                                    cGGCCAATCTATGACACAGTCGATTGTCTATATTTATGATCCCCCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCAGCTACGCCACCCTCGTTCTAGCCGGCTTGGATCATAGCTTTTAGGCGATTGAAGCAACgctgc                      <  1:1331854/140‑1 (MQ=255)
                                                                                                                    cGGCCAAATTATGACACAGTCGATTGGCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACgctgc                      >  1:1767747/1‑140 (MQ=255)
                                                                                                                    cGGCCAAATTATGACACAGTCGAGTTTGTATATTTATTATAAAGCCCGCAATGTTGCCGGAGCCCTGTTCCGCGGCCTCTACTCCCCCATAGTTCTATACGGCTTGCATCATAGCTTTTATGCGATTGAATCAAcccggc                      >  1:1833516/1‑135 (MQ=255)
                                                                                                                                   acaGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCgtg       <  1:3449392/140‑1 (MQ=255)
                                                                                                                                     aGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCCTCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAAAGCTGCTTTCTGATACGCGTGTc     >  2:6329331/1‑140 (MQ=255)
                                                                                                                                      gTCGATTGTCTATATTTATTATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTAATCCACCATCGTTATATCCGGCTTTCATCATAGCTTTTATGCGATTTAAGCAAAGCTGGTTTCTTATCCGCGTGTCg    >  1:4678428/1‑140 (MQ=255)
                                                                                                                                       tCGATTGTGTATATTTATGATCACCCCCGCCTTGTTTCCGGAGCCCTGTTCGGCGGCATCTAATCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATTCGATTGAAGCAACGCTGCTTTCTGATACGCGTGTCgg   >  1:6778803/1‑140 (MQ=255)
                                                                                                                                       tCGAGTGTCTATATTTATGATCACCCCGGCATTGTTTCCGGAGCCCTGTTCGGCGGCATATACTCCACCATCGTTATATACGGCTTGCATCATAGCTTTTATTCGATTGAAGCAAACCTGCTTGCTGATCCCCGTGTCgg   >  1:4422378/1‑140 (MQ=255)
                                                                                                                                        cGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGc  <  2:2662199/140‑1 (MQ=255)
                                                                                                                                        cGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGc  <  1:2433543/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                        
AAGCTATTAAAATCAATTGTGCCGAAGTCGCTTGATGTCGTCATCATTCCTTTTATTACGGTCATGGTGACAGGGTGCCTCGCGCTGATTGTGATGAATCCCGCCGCATCTATCATCGGCCAAATTATGACACAGTCGATTGTCTATATTTATGATCACGCCGGCATTGTTGCCGGAGCCCTGTTCGGCGGCATCTACTCCACCATCGTTCTATCCGGCTTGCATCATAGCTTTTATGCGATTGAAGCAACGCTGCTTGCTGATCCGCGTGTCGGC  >  NZ_CP034943/3769437‑3769712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: