BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,198	G→T	16.4%	Q112K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,198	0	G	T	16.4%	1325.4 / 88.0	680	Q112K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (229/316); new base T (133/2); total (362/318)
Fisher's exact test for biased strand distribution p-value = 1.07e-39
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 693 total aligned reads displayed.

GTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAT  >  NZ_CP034943/240059‑240337

     gaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTGAAGTTTTTGgaga                                                                                                                                        >  1:2706859/1‑140 (MQ=255)

         gtgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTg                                                                                                                                    <  1:3059358/140‑1 (MQ=255)

          tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGa                                                                                                                                   >  2:2005845/1‑140 (MQ=255)

           gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTTaa                                                                                                                                  >  2:787737/1‑140 (MQ=255)

           gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                                                  <  2:2901672/140‑1 (MQ=255)

           gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTTGGAGAttttta                                                                                                                                  >  1:845063/1‑137 (MQ=255)

             aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGga                                                                                                                                          >  1:3886478/1‑130 (MQ=255)

             aaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATg                                                                                                                                >  2:460719/1‑140 (MQ=255)

                  agagagaAGGAGCATCTCTGAGGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATCCGGGCGTGTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCttt                                                                                                                           >  2:3995270/1‑140 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAATTTTTGGAGATTTTAATTGCtttt                                                                                                                          >  1:280526/1‑140 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGATATTTGAATTGCtttt                                                                                                                          >  2:5168880/1‑140 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGGtttt                                                                                                                          >  1:96996/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATATGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                         >  1:1217368/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTTGGTGTTTTGAATTGCTTTTc                                                                                                                         >  1:4564611/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATTGCTTTTc                                                                                                                         >  1:6444587/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAACTTTTTGGAGATTTGAATTGGTTTTc                                                                                                                         >  1:1009165/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAATTTTTTGGAGATTTTAATTGCtttta                                                                                                                         >  1:1421744/1‑139 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGGAGTGTGGGGATGTTTGAATGGCTTTTc                                                                                                                         >  2:4172386/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGAGATTTGAATTGCTTTTc                                                                                                                         >  2:1854831/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGGGATTTGAATGGCTTTTc                                                                                                                         >  1:1599556/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTc                                                                                                                         >  2:2312554/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAATTTTTGGAGATTTGAATTGCTTTTc                                                                                                                         >  1:3609267/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAATTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                                         >  2:5322955/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAATTTTTTTGAGATTTTAATTGCtttta                                                                                                                         >  1:6329427/1‑139 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTTAGTTTTTGGAGATTTTAATGTCttttg                                                                                                                         >  1:528041/1‑139 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTTAATTTTTGGGAGATTTGAATGGCtttta                                                                                                                         >  1:6895545/1‑139 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCtttta                                                                                                                         >  2:2493337/1‑139 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTTAATGGGTTTTc                                                                                                                         >  1:1743435/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGGTGGAATTATTCAGAGATGCTGTTTGTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGCCTATACGGGCGGTTTCGGATTTTTGTTTTCAGTTTTGGGTGATTTGAATGGGTTTTc                                                                                                                         >  1:6680266/1‑140 (MQ=255)

                    agagaAGGAGCAACTCTGATGTTGGAATTATTCAGAGAGGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGGTTGAAGTTTTGGGCGATTTTAATTGGttttg                                                                                                                         >  1:3396252/1‑139 (MQ=255)

                      agaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCg                                                                                                                       >  1:4885418/1‑140 (MQ=255)

                              cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTaaa                                                                                                               >  2:6127656/1‑140 (MQ=255)

                                 ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                            <  2:626791/140‑1 (MQ=255)

                                 ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                            <  1:1515958/140‑1 (MQ=255)

                                     gATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAagag                                                                                                        <  1:6226218/140‑1 (MQ=255)

                                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGTTTTGAATTGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                       >  2:1055703/1‑140 (MQ=255)

                                      aTGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAgg                                                                                                       <  1:4177820/140‑1 (MQ=255)

                                        gTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTGAATTGCTTTTACGTTTTTAAAACAAGAGGCa                                                                                                     >  1:4097825/1‑140 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  2:7476579/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  1:5399441/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  1:2005845/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  1:2914009/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  1:7027387/140‑1 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATTGGTTTTGCGTTTTTAAAAAAAAAGGCaaca                                                                                                  >  1:223180/1‑138 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGTCCAGc                                                                                                  >  2:7097233/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTACGTTTTTAAAACAATAGGCAAGc                                                                                                  >  1:1343283/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGc                                                                                                  >  1:7449755/1‑140 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                            <  1:4373381/140‑1 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                            <  2:2747906/140‑1 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATa                                                                                            >  2:3263121/1‑140 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGATGCAAGCCAGATAAAACCGacaaa                                                                                 >  2:5831470/1‑139 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCACATAAACCCGacaac                                                                                 >  2:3511529/1‑140 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAATCCAGATAAACCCGacaaa                                                                                 >  2:7014208/1‑139 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGTCTTTTCCGTTTTTAAAAAAACAGGCAAGCCAGATAAACCCGacaac                                                                                 >  2:6903309/1‑140 (MQ=255)

                                                            cTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACAATACGGGCGTTTGCGGATTTTTGTTTGAAATTTTGTTAGAGTTTAAATGATATTCCGTATTTAATACAATATGCAACCCAGAGAAACACGaaaac                                                                                 >  2:3012426/1‑140 (MQ=255)

                                                               tttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaac                                                                              >  2:5002809/1‑140 (MQ=255)

                                                                ttttGTATTTTTCTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGacaacaaca                                                                             <  2:954884/140‑1 (MQ=255)

                                                                 tttGTATTTTTGTTCTAATAAGAATTAGATAAATCAGACTATACGTGCGTTTTATTATTTTTGTTTGAATTTTTGTGAGATTTGAATTGATTTTCCGTTTTTAAAACAATATGCAAGACAGATAAACCCGAcaacaaaaa                                                                            >  2:4725684/1‑140 (MQ=255)

                                                                       tttttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   >  2:3727369/1‑111 (MQ=255)

                                                                       tttttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAg                                                                                                   <  1:3727369/111‑1 (MQ=255)

                                                                         tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                    <  2:5569947/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGCGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:3511529/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:113777/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:5964741/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  2:3337870/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  2:6794321/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:2144288/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  2:1305830/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGAATATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:7574732/140‑1 (MQ=255)

                                                                             gtCTAACACGCAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTGCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  1:5056337/139‑1 (MQ=255)

                                                                                  aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGCAGGGCTCTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                           <  1:7714780/140‑1 (MQ=255)

                                                                                  aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAATAGGCAAGCCATATAAACCCGACAACAACAAAAAATAAACTAGCAAGt                                                           >  2:7574732/1‑140 (MQ=255)

                                                                                  aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                                           <  1:7648575/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  1:2464572/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTGTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  2:1480494/140‑1 (MQ=255)

                                                                                     cGAAGTCGATCATTCCGAATATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTGCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCTACAACAACAAAAAATAATCTAGCAAGTaaa                                                        <  1:4692766/140‑1 (MQ=255)

                                                                                       aaGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAAAAGAGGCAAGCCAGATAAACACGACAACAACAAAAAATAATCTATCAAGTACACt                                                      >  2:4263526/1‑140 (MQ=255)

                                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                     <  2:4885418/140‑1 (MQ=255)

                                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                     <  1:241593/140‑1 (MQ=255)

                                                                                         gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                                                    <  2:1692349/140‑1 (MQ=255)

                                                                                              tgataccGACTATACGGGCGTGTTCCGATTTTTGTTTGAAGCTTTGCGAGCTGTGAATGGCTTTGCCGTTTGTAAAACAACAGGCAAGCCAGATAAACCCGACAACAACAAAATATACTCTAGCATGTAAACTCcatgca                                               <  2:766351/135‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:1875468/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:5696921/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:882316/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:3284304/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:4967829/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTGTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:5797956/140‑1 (MQ=255)

                                                                                                 ttCCGACTATCCGGGCTTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAGCTCCATGCATgg                                            <  1:7156187/140‑1 (MQ=255)

                                                                                                      aCTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCCGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTaa                                       >  2:6484276/1‑140 (MQ=255)

                                                                                                      aCTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTaa                                       >  2:3120799/1‑140 (MQ=255)

                                                                                                            cGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCACAACAACAAAAAATAATATAGCAAGTAAACTCCATGCATGGGGTAattcatt                                 >  2:2143499/1‑140 (MQ=255)

                                                                                                               gCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTACATGCATGGGGTAATTCATTCGt                              >  2:7179781/1‑140 (MQ=255)

                                                                                                                cGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGtt                             >  2:5940255/1‑140 (MQ=255)

                                                                                                                    ttCTGATTTTTGTTTGAAGTTTTGTGAGATTTGAATTGATTTTCCGTATTTAAAACAAGAGGCAAGCCAGATAAACCCGACAAAAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                         <  2:4451348/140‑1 (MQ=255)

                                                                                                                    ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                         <  2:6012247/140‑1 (MQ=255)

                                                                                                                    ttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                         <  2:2575295/140‑1 (MQ=255)

                                                                                                                               gtttgAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt              <  1:5483603/140‑1 (MQ=255)

                                                                                                                                 ttgAATTTTTTGGAGATTTGAATGGCTTTTAATATTTTAAAAAAAGAGTCAAGCCATATAAACCATAAAAAAACAAAAAATAATATATAAATTAAACTACATGCTTGGGTTAATTAATTAGTTGCAAAATATAAAAATag            <  2:6694593/140‑1 (MQ=255)

                                                                                                                                    aaGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt         <  2:1206934/140‑1 (MQ=255)

                                                                                                                                    aaGTTTTGGGAGATTTGAATGGATTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGt         <  1:6084640/140‑1 (MQ=255)

GTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAT  >  NZ_CP034943/240059‑240337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`