BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,945,908	T→G	7.4%	L388R (CTT→CGT)	EO946_RS20305 →	Na+/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,945,908	0	T	G	7.4%	946.6 / 3.2	473	L388R (CTT→CGT)	EO946_RS20305	Na+/H+ antiporter
Reads supporting (aligned to +/- strand): ref base T (229/170); new base G (59/2); total (301/172)
Fisher's exact test for biased strand distribution p-value = 4.27e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 494 total aligned reads displayed.

CCGGTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTC  >  NZ_CP034943/3945769‑3946047

  ggTGACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCATGCCTAGAGACTTCCCGTGACGTGAAGAGATTCTGGTTTTCGCCTTCAGTGTCTTTTTGTTTTCCCttg                                                                                                                                           <  1:5348620/140‑1 (MQ=255)

   gTTACAGGGAAGCATACCATCAAGTGGGGCGGGCTGAAGGGTTCTCTGTCCAGTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttgt                                                                                                                                          <  2:6945480/140‑1 (MQ=255)

       cATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGGCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTgttgtt                                                                                                                                      >  2:1238844/1‑140 (MQ=255)

        aTGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTc                                                                                                                                     <  1:2861304/140‑1 (MQ=255)

               cATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  1:753304/140‑1 (MQ=255)

                aTACCATCACTTGGGGCGGGCTGAAGGGTTCTCTGTCCATTTCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTc                                                                                                                             <  1:6982921/140‑1 (MQ=255)

                 tACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCACTTGTTGTTAAGGGGCTCa                                                                                                                            >  2:6493833/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGATACTTCCCGGGACGTGAATACATTCTGGTTTTCGCCTTCATTGTCGTTTTGTTTTACCTTGTTGTTAATGGGCTAAc                                                                                                                           >  2:3865686/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTAACTTGTTGTTCAGGGGCTCAc                                                                                                                           >  2:2316295/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttg                                                                                                                                           <  1:4675937/124‑1 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCAc                                                                                                                           >  2:4779921/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACGTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGGGGTGGTCAGGGGCTCAc                                                                                                                           >  1:1135219/1‑140 (MQ=255)

                  aCCATCAATTGGGGCGCGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGACTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTACAGTGTCTTTTTGTTTACCCTTGTGGTTCAGGGGCacac                                                                                                                           >  1:1653418/1‑140 (MQ=255)

                  aCCATCAATTGGGGAGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGAATTCCCGGGACGTGAAGACATTCTGTTTTTCGCCTTCAGTGTCGTTTTTTTTTAACTTGTTTTTCAGGGGcgaaa                                                                                                                           >  1:7229905/1‑136 (MQ=255)

                          ttGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGc                                                                                                                   <  2:2499021/140‑1 (MQ=255)

                          ttGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGc                                                                                                                   <  2:6247848/140‑1 (MQ=255)

                          ttGGGGCGGGCGGAAGGGTTCTCGGTCCAGTGCACTCGTGCTGAGCCTGCCTAGAGACGTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGc                                                                                                                   <  2:4481261/140‑1 (MQ=255)

                             gggcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGAGATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCga                                                                                                                <  1:1731150/140‑2 (MQ=255)

                             gggcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGc                                                                                                                <  1:6812412/140‑1 (MQ=255)

                               gcgggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTACCTTGTTGTTGAGGGGCTCACCATCAACCCGctt                                                                                                              >  1:2361372/1‑140 (MQ=255)

                                  ggcTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGcttctt                                                                                                           >  2:5928817/1‑140 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:7343424/140‑1 (MQ=255)

                                    cTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  1:1479368/140‑1 (MQ=255)

                                     ttaaGGGTTCTCTGTCCATTTCACTCTTGCTTAGCCTGACTAGAGAATTCCCGGGACTTGAATACATTCGTGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGaa                                                                                                        <  2:301071/138‑1 (MQ=255)

                                     tGAAGGGTTCTCTGTCCATTGCACTCGTTCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTTTCGTTTTGTTTTCCCTTGTTGTTCACGGGCTCACCATCAAGCCGCTTATTGaa                                                                                                        <  2:1108271/140‑1 (MQ=255)

                                     tGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGaa                                                                                                        <  2:6334197/140‑1 (MQ=255)

                                     tGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGaa                                                                                                        <  2:2295359/140‑1 (MQ=255)

                                         ggtttCTCTGTCCATTGCACTCGTGATGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGTTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGt                                                                                                    <  1:7649697/137‑1 (MQ=255)

                                         gggTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGt                                                                                                    <  1:4052841/140‑1 (MQ=255)

                                         gggTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGt                                                                                                    <  1:2241285/140‑1 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTGTTTTTCAGGGGCTCACCAACAAACCGCGTCTTGAACGGTTAgg                                                                                                >  1:203837/1‑140 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAgg                                                                                                >  2:5471680/1‑140 (MQ=255)

                                             tctcTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTGAACGGTTggg                                                                                                >  2:5199804/1‑140 (MQ=255)

                                              ctctGTCCATTGCACTCGTGCTGATCCTGCCTAGAGACTTCCCGGGACTTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTAACTTGTTTTTCAACGGCTCAACATAAAGCCGCATATTGAAAGCTTAGgt                                                                                               >  2:5538704/1‑140 (MQ=255)

                                              ctctGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTACCTTGTTGTTCATGGGCGCACCATAAACCCGCTTCTTTTACGGGTAGgt                                                                                               >  1:2074213/1‑140 (MQ=255)

                                                   tCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTGTTGTGGGGCTCACCCTCAAGCCGGGTGGTGCACGGTTAGGtgtgga                                                                                          >  1:4955756/1‑138 (MQ=255)

                                                   tCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCGTGAACGGTTAGGTGTGaa                                                                                          >  1:1691968/1‑140 (MQ=255)

                                                    ccATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAATCCGCTTCTTGAACGGTGATGTGTGAAt                                                                                         >  2:1554830/1‑140 (MQ=255)

                                                    ccATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCATGTTGTTCAGGGGCTCACCATCAAGCCGCGTCTTGAAAGGTTAGGTGTGAAt                                                                                         >  2:2122233/1‑140 (MQ=255)

                                                    ccATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAAt                                                                                         >  2:4271489/1‑140 (MQ=255)

                                                     cATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATc                                                                                        >  2:5305410/1‑140 (MQ=255)

                                                      aTTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTAAGGGGCTCACCAAAAAACCGCTTCTTGAACGGTTAtgtggttatca                                                                                       >  2:3625175/1‑133 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGTAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTTTTGTTCAGGGGCTCACCATCAAGACGCTTCGTGAACGGGTAGGTGTGAATCaaa                                                                                     >  2:7727942/1‑140 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTTTTTAGGGGCTCCCCAACAAACCGCGGGTTGGACGGTTGGGtgtgtctaaaa                                                                                     >  1:5917369/1‑133 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCaaa                                                                                     >  2:7278207/1‑140 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCaaa                                                                                     <  2:4655956/140‑1 (MQ=255)

                                                        tGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTGCCGTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCaaa                                                                                     >  1:6191915/1‑140 (MQ=255)

                                                         gCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAATCCGCTTCTTGAACGGTTAGGTGTGAATCaaaa                                                                                    >  2:6666529/1‑140 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              <  1:7442637/94‑1 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaa                                                                                >  2:4132533/1‑140 (MQ=255)

                                                             tCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCGTGAACGGTTAGGTGTGAATCAAAaagaa                                                                                >  2:5116058/1‑140 (MQ=255)

                                                                 gCTGAGCCTGCCTAGAGACGTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAaagaagaag                                                                            <  2:2395606/140‑1 (MQ=255)

                                                                  cTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCTTGTTGTTAAGGGGCTCACCATAAAGCCGCTTATTGAACGGTTAGGTGTGAATCAAAAATAAAAAgg                                                                           >  1:2592825/1‑140 (MQ=255)

                                                                   tGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGc                                                                          <  2:6094985/140‑1 (MQ=255)

                                                                     aGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTCCCTTGTTGTTGAGGGGGTCACCATCAAGCCGCGTCTTGTAAGGTGGGGTGTGAATCAAAAAGAAGAAGGGaa                                                                        >  1:5458714/1‑140 (MQ=255)

                                                                       cctgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttgt                                                                                                                                          <  1:4528831/72‑1 (MQ=255)

                                                                        ctgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCATTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCa                                                                     <  2:700304/140‑1 (MQ=255)

                                                                         tgcctAGAGACTTCCCGGGACGTGACGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCCCCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                    <  1:5726611/140‑1 (MQ=255)

                                                                         tgcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAg                                                                    <  1:1002749/140‑1 (MQ=255)

                                                                         tgcctAGAGACATCCCGGGACCTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCCAGCCGCTTCTTGAACGGTTAGGTGTGAATAAAAAAGAAGAAGGCAACCAg                                                                    <  2:3529688/140‑1 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAgg                                                                   >  2:3417159/1‑140 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTAAAATGTTGTTGAAGGGGTCAACAACAAAACGCGAAATTAACCGGTAGGTGTGAATCAAAAATAAAAAAGAAACCact                                                                   >  1:3222307/1‑138 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGa                                                                                                         <  2:1670923/102‑1 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAgg                                                                   >  2:5744423/1‑140 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCGTGTTGTTCAGTGGCTCACAATAAAGCCGCTTCTTTAACGGAAAGGTGTGAAACAAAAAGAAGAAGGCAACCAgg                                                                   >  2:878602/1‑140 (MQ=255)

                                                                          gcctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGGTGTTTTCCCGTGGTGTTCAGGGGCGCACCAACAAGCCGCGTGTTGAACGGGTAGGGGGGTATCAAAAAGAACAAGGCCACCAgg                                                                   >  1:3127955/1‑140 (MQ=255)

                                                                           cctAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTACCGTGTTTTTGCGGGGCGCACCAACAACCCGCGTCTTGGACGGGTAGGGGGGAATAAAAAAAAATAAAGCAACCaaca                                                                  >  1:893624/1‑137 (MQ=255)

                                                                               gagaCTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTTCCTTTTTGTTTAGGGGGTCACCATAAAGCCGCTTCTTGTACGGTGTGGTGTGTATAAAAAATAAGAAGGCAAACAGGGGTAt                                                              >  1:709411/1‑140 (MQ=255)

                                                                               gagaCTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCttgt                                                                                                                                          <  2:4396867/64‑1 (MQ=255)

                                                                                 gaCTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCAACAAGCCGCGTCTTGAACGGTTAGGGGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGa                                                            >  1:3396776/1‑140 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTGAGGGGCTCACCATGAAGCCGCTTCTTGAACGGGTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGGATGAGGa                                                         >  2:4058753/1‑140 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGGTAGGGGTGAATCAAAAAGAATAAGGCAACCAGGATTATGAGGa                                                         >  2:1911904/1‑140 (MQ=255)

                                                                                    ttCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCACCCTCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGa                                                         >  2:2781849/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTAACTTTTTGTTCATGGGCTCACCATCAAGCCGATTGTTTAACAGTTAGGTGTGAATCAAAAAATAAAAAGCAACCAAGAGTATGATGaa                                                        >  1:6699583/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCGTGTTGTTCAGGGGGTCACCCACAAGCCGCGTCTTGAACGGTTAGGTGTGAATAAAAAAGAAGAATGCAAACACGAGTAAGAGGaa                                                        >  1:352246/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTTAACGGTTAGGTGTGAATAAAAAAAAAGAAGGCAACAAAGAATATTAGGaa                                                        >  2:4985543/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTGAGGGGCGCACCAACAAGCCGCTTCGTGTACGGGTAGGTGTGAATAAAAAATAAGAAGGCCACCACGCGGATGAGGaa                                                        >  1:2928958/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCTTGTTGTTCAGGGGCTCACCATAAGACCGCTTCTTGTACGGGTAGGGGTGAATAAAAAAATAGAACGCAACCAGGAGTAGGAGGaa                                                        >  1:4247430/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTACCGTGTTGTTGAGGGGCTCACCAACAAGCCGCGTCTTGTACGGGTAGGTGTGTATAAAAAAGAAGAACGCCACCAGGCGGATGGGGaa                                                        >  1:2115559/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTGTTGTTTTGACGTGTTTTTCAGGGGCGCACCATCAAGCCGCTTCTTTCAAGGGGAGGTTTGAATCAAAAAGAAGAAGGCAAACACGAGTTTGAGGaa                                                        >  1:4126188/1‑140 (MQ=255)

                                                                                     tCCCGGGACGTGAAGACAGTCTGGTTTTCGCCTTCAGTGTCGTTGTGTTTTGCCTTTTTTTTCCGGGGCTAAACAGCAAGCCGCGTCTTGGACGGTTATGGGGGAATCAAAAAGAAGGAGGCCACCACGTGTATGAGGaa                                                        >  1:1243309/1‑140 (MQ=255)

                                                                                      cccGGGACGTGAATACATTCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTCACTTTTTGTTCATGGGCTCACCATCAAGCCGCTTATTGAACGGTTATGTGTGAATCAAAAAGAATAAGGAAAACAGGAGTATGATGAAt                                                       >  2:5398835/1‑140 (MQ=255)

                                                                                          ggACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgct                                                   <  2:2601579/140‑1 (MQ=255)

                                                                                          ggACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgct                                                   <  2:1496658/140‑1 (MQ=255)

                                                                                            aCGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAAttgcttg                                                 <  2:7421221/140‑1 (MQ=255)

                                                                                                gAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTGCCGTGTTGTTGAGGGGCTCACCATCAAGCCCCTTCTTGTACGGGTAGGTGTGAATAAAAAATAATAAGGCCACCACGAGTATGAGGAAATTGTTGCaaa                                             >  1:2470292/1‑140 (MQ=255)

                                                                                                     cATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGAc                                        <  2:2073721/140‑1 (MQ=255)

                                                                                                       ttCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACAt                                      <  1:5116058/140‑1 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTTTTTTGCCTTGTTTTTGAGGGGGTCACCATCAAGCCGCTTGGTGAACGGTTTGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATTAGGAAATGCTTGCAAAAGGACATc                                     >  1:5217492/1‑140 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCt                                                                                                                              >  2:2898692/1‑51 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTGTTGAACGGTTAGGTGTGAATCAAAAATAAGAATGCAAACAGGAGTATGAGGAATTGCTTGCAAAAGGaaaac                                     >  2:1031236/1‑136 (MQ=255)

                                                                                                        tCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGa                                                                  <  1:4432879/111‑1 (MQ=255)

                                                                                                        tCTGGTTATCGCCTTCAGTGTCGTTTTGTTTTCCCTGTTTTTTAAGCGGCTCACCAACAAACCGCTTGTTGAAAGGGTACGGGTGAATCAAAAAATATAAGGCAAACAGGAGTATGAGGAATATCGTGCAAAAAGACaac                                     >  2:2028423/1‑138 (MQ=255)

                                                                                                                tCGCCTTCAGTGTCGTTTTTTTTTTCCGTGTTGTTGAGGGGCTCACCCAAAAGCCGCGTCGTGTACGGGTAGGTGTGAATCAAAAAAAAGAAGGCAAACACGAGGATGAGGAATTTCTTTGCAAATGACAACGCGTAGaa                             >  1:6375620/1‑140 (MQ=255)

                                                                                                                  gCCTTCAGTGTCGTTTTGTTTTACCAAGTTGTTCAGGGGCAAACCAAAAAGCCGCATAGTTAACCGGTATGTGTGTATCAAAAATAAGAATGCAAACAGGAGTATGAGTAATAGATATAAAAAAGACATAGATTACAAAc                           >  2:3187449/1‑140 (MQ=255)

                                                                                                                             tgttttgttttCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAaag                <  2:1693890/139‑1 (MQ=255)

                                                                                                                                      ttCCGTTGTTGTTCAGGGTCTCACCATGCAGCCGCTTCTTGAATGTTTATGTGTGAATCAAAAAGTAGATGGCATACAGGAGTATTATGAATTGCTTGCAAAATTACATCGCTTAGAAACCGCCATCAAAGAAGTCCatc       <  2:4563694/140‑3 (MQ=255)

                                                                                                                                      gtCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGACCGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGc       <  1:3800368/139‑1 (MQ=255)

                                                                                                                                         ccTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGc                                                                          <  2:7530617/70‑1 (MQ=255)

                                                                                                                                         ccTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGc                                                                          >  1:7712129/1‑70 (MQ=255)

CCGGTTACATGGAAGCATACCATCAATTGGGGCGGGCTGAAGGGTTCTCTGTCCATTGCACTCGTGCTGAGCCTGCCTAGAGACTTCCCGGGACGTGAAGACATTCTGGTTTTCGCCTTCAGTGTCGTTTTGTTTTCCCTTGTTGTTCAGGGGCTCACCATCAAGCCGCTTCTTGAACGGTTAGGTGTGAATCAAAAAGAAGAAGGCAACCAGGAGTATGAGGAATTGCTTGCAAAAGGACATCGCTTAGAAACCGCCATCAAAGAAGTCCAGCAAGTC  >  NZ_CP034943/3945769‑3946047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`